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DSBs are repaired by two major pathways termed homologous recombination and non-homologous end-joining (NHEJ). NHEJ is responsible for repairing the vast majority of DSBs arising in human cells. Defects in NHEJ factors are also associated with microcephaly, primordial dwarfism and immune deficiencies. One of the key proteins important for mediating NHEJ is XRCC4. XRCC4 is a dimer, with the dimer interface mediated by an extended coiled-coil. The N-terminal head domain forms a mixed alpha\u2013beta globular structure. Numerous factors interact with the C-terminus of the coiled-coil domain, which is also associated with significant self-association between XRCC4 dimers. A range of construct lengths of human XRCC4 were expressed and purified, and the 1\u2013164 variant had the best NMR properties, as judged by consistent linewidths, and chemical shift dispersion. In this work we report the <jats:sup>1<\/jats:sup>H, <jats:sup>15<\/jats:sup>\u00a0N and <jats:sup>13<\/jats:sup>C backbone resonance assignments of human XRCC4 in the solution form of the 1\u2013164 construct. Assignments were obtained by heteronuclear multidimensional NMR spectroscopy. In total, 156 of 161 assignable residues of XRCC4 were assigned to resonances in the TROSY spectrum, with an additional 11 resonances assigned to His-Tag residues. Prediction of solution secondary structure from a chemical shift analysis using the TALOS\u2009+\u2009webserver is in good agreement with the published X-ray crystal structures of this protein.<\/jats:p>","DOI":"10.1007\/s12104-021-10035-6","type":"journal-article","created":{"date-parts":[[2021,6,25]],"date-time":"2021-06-25T19:03:31Z","timestamp":1624647811000},"page":"389-395","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["1H, 13C, 15N backbone resonance assignment for the 1\u2013164 construct of human XRCC4"],"prefix":"10.1007","volume":"15","author":[{"given":"Maria Jose","family":"Cabello-Lobato","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8363-7933","authenticated-orcid":false,"given":"Christine K.","family":"Schmidt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7482-0234","authenticated-orcid":false,"given":"Matthew J.","family":"Cliff","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,6,25]]},"reference":[{"key":"10035_CR1","doi-asserted-by":"publisher","first-page":"566","DOI":"10.1038\/nature18643","volume":"535","author":"I Brouwer","year":"2016","unstructured":"Brouwer I, Sitters G, Candelli A, Heerema SJ, Heller I, Zhang H, Normanno D, Modesti M, Peterman EJG, Wuite GJL (2016) Sliding sleeves of XRCC4-XLF bridge DNA and connect fragments of broken DNA. 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