{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T13:42:27Z","timestamp":1784641347896,"version":"3.55.0"},"reference-count":21,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2024,3,27]],"date-time":"2024-03-27T00:00:00Z","timestamp":1711497600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2024,3,27]],"date-time":"2024-03-27T00:00:00Z","timestamp":1711497600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"National Hospital"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Head and Neck Pathol"],"abstract":"<jats:title>Abstract<\/jats:title><jats:sec>\n                <jats:title>Background<\/jats:title>\n                <jats:p>The detection of human papillomavirus (HPV) has several implications in the diagnostic work-up and management of oropharyngeal squamous cell carcinoma (OPSCC). The choice of HPV detection assay and testing algorithms differ across institutions and vary in cost, detection targets, technical feasibility, and turnaround time. In this study, we aimed to validate the VisionArray\u00ae HPV Chip for formalin-fixed and paraffin-embedded (FFPE) samples of OPSCC using the previously applied standard pan-HPV DNA PCR assay as a reference.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>The validation cohort consisted of FFPE tissue samples from patients previously diagnosed with HPV DNA-positive OPSCC (n\u2009=\u200980), HPV DNA-negative OPSCC (n\u2009=\u200921), and a benign group of tumor samples consisting of Warthin\u2019s tumors (n\u2009=\u200920) and branchial cleft cysts of the lateral neck (n\u2009=\u200914). All samples were tested with p16 immunohistochemistry, pan-HPV DNA PCR, and the VisionArray\u00ae HPV Chip.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>The overall sensitivity and specificity of the VisionArray\u00ae HPV Chip assay were 100% [95% CI 95.5%; 100.0%] and 96.3% [95% CI 87.3%; 99.6%] and the positive predictive value and negative predictive value were 97.6% [95% CI 91.5%; 99.7%] and 100% [95% CI 93.2%; 100%], respectively.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>The VisionArray\u00ae HPV Chip assay can be recommended for high-risk HPV testing in FFPE tissue samples from OPSCC, providing both a fast and simultaneous genotyping for 41 clinically relevant HPV types.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1007\/s12105-024-01628-3","type":"journal-article","created":{"date-parts":[[2024,3,27]],"date-time":"2024-03-27T13:03:37Z","timestamp":1711544617000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Validation of the VisionArray\u00ae Chip Assay for HPV DNA Testing in Histology Specimens of Oropharyngeal Squamous Cell Carcinoma"],"prefix":"10.1007","volume":"18","author":[{"given":"Hani Ibrahim","family":"Channir","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simone Kloch","family":"Bendtsen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linea Cecilie","family":"Melchior","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pia Rovsing","family":"Sandholm","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"Mordhorst","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amanda-Louise Fenger","family":"Carlander","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"von Buchwald","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katalin","family":"Kiss","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,3,27]]},"reference":[{"key":"1628_CR1","doi-asserted-by":"publisher","first-page":"3115","DOI":"10.1007\/s00405-021-07133-5","volume":"279","author":"HI Channir","year":"2022","unstructured":"Channir HI, Lomholt AF, Gerds TA et al (2022) Human papillomavirus testing in metastatic squamous cell carcinoma of the neck with unknown primary using PCR on fine-needle aspiration smears: a prospective clinical study. Eur Arch Oto Rhino Laryngol 279:3115\u20133121","journal-title":"Eur Arch Oto Rhino Laryngol"},{"key":"1628_CR2","doi-asserted-by":"publisher","first-page":"1067321","DOI":"10.3389\/fonc.2022.1067321","volume":"12","author":"EA Mensour","year":"2022","unstructured":"Mensour EA, Alam S, Mawani S et al (2022) What is the future of treatment de-escalation for HPV-positive oropharyngeal cancer? A review of ongoing clinical trials. Front Oncol Front Oncol 12:1067321","journal-title":"Front Oncol Front Oncol"},{"key":"1628_CR3","doi-asserted-by":"publisher","first-page":"239","DOI":"10.1016\/S1470-2045(23)00013-X","volume":"24","author":"H Mehanna","year":"2023","unstructured":"Mehanna H, Taberna M, von Buchwald C et al (2023) Prognostic implications of p16 and HPV discordance in oropharyngeal cancer (HNCIG-EPIC-OPC): a multicentre, multinational, individual patient data analysis. Lancet Oncol 24:239\u2013251","journal-title":"Lancet Oncol"},{"key":"1628_CR4","doi-asserted-by":"publisher","first-page":"559","DOI":"10.5858\/arpa.2017-0286-CP","volume":"142","author":"JS Lewis","year":"2018","unstructured":"Lewis JS, Beadle B, Bishop JA et al (2018) Human papillomavirus testing in head and neck carcinomas guideline from the college of american pathologists. Arch Pathol Lab Med 142:559\u2013597","journal-title":"Arch Pathol Lab Med"},{"key":"1628_CR5","doi-asserted-by":"publisher","first-page":"820","DOI":"10.1002\/cncy.21753","volume":"124","author":"HI Channir","year":"2016","unstructured":"Channir HI, Gr\u00f8nh\u00f8jLarsen C, Ahlborn LB et al (2016) Validation study of HPV DNA detection from stained FNA smears by polymerase chain reaction: Improving the diagnostic workup of patients with a tumor on the neck. 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VisionArray\u00ae is a registered trademark by ZytoVision. The company had no role in the funding or in the conduction of the study.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}},{"value":"All procedures performed involving human participants were in accordance with the ethical standards of the institutional and\/or national research committee and with the 1964\u00a0Helsinki declaration\u00a0and its later amendments or comparable ethical standards. Use of patient material was approved by the local ethics committee at the Capital Region of Denmark (H-20072877) and the Data Protection Agency (P-2021-676).","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical Approval"}},{"value":"For this type of study, the informed consent is not required.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Informed Consent"}},{"value":"For this type of study, the informed consent is not required.","order":5,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent to Participate"}},{"value":"Not applicable.","order":6,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent to Publish"}}],"article-number":"27"}}