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Its diagnosis is challenging owing to its bland morphology, which makes differentiation from non-neoplastic lesions difficult on biopsy. Despite this, CC often requires distinction from conventional OSCC, even in surgical specimens. Histological overlap with conventional OSCC suggests a continuous spectrum of diseases. To clarify the genetic identity of pure CC, we analyzed 23 cases of OSCC with burrowing invasive patterns and classified them into three groups: CC, conventional OSCC (SCC), and uncertain CC (UCC).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We retrospectively reviewed 2002 OSCC cases from multicenter archives. From this cohort, 23 cases exhibiting a burrowing invasive pattern characteristic of CC were selected. The cases were classified into the three study groups based on the WHO diagnostic criteria, and clinicopathological features were reviewed. Targeted next-generation sequencing was performed to identify clinically significant genetic alterations, and immunohistochemical staining was also conducted.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The 23 cases were histologically classified into CC (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20098), UCC (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20097), and SCC (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20098) groups. Clinically, the CC group predominantly affected the gingiva with no recurrence or metastasis. Conversely, the SCC group primarily involved the tongue and showed recurrence and metastasis. Genetic alterations were detected in 87.5% (7\/8) of CC cases, with low frequencies of\n                      <jats:italic>TP53<\/jats:italic>\n                      (12.5%) and\n                      <jats:italic>CDKN2A<\/jats:italic>\n                      (25.0%) alterations and higher frequencies of\n                      <jats:italic>FAT1<\/jats:italic>\n                      (50.0%),\n                      <jats:italic>NOTCH1<\/jats:italic>\n                      (37.5%),\n                      <jats:italic>PIK3CA<\/jats:italic>\n                      (37.5%), and\n                      <jats:italic>CASP8<\/jats:italic>\n                      (50.0%) alterations. In contrast, the SCC group showed frequent\n                      <jats:italic>TP53<\/jats:italic>\n                      (75.0%) and\n                      <jats:italic>CDKN2A<\/jats:italic>\n                      (75.0%) alterations, whereas\n                      <jats:italic>FAT1<\/jats:italic>\n                      and\n                      <jats:italic>NOTCH1<\/jats:italic>\n                      alterations were absent. Consistent with these findings, p53 staining revealed wild-type patterns in the CC group along with a lower Ki-67 labeling index. The UCC group exhibited intermediate clinicopathological and genetic characteristics.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>This study provides the first genetic characterization of oral CC, distinct from that of conventional OSCC. This genetic signature may contribute to the biological behavior of CC and offer a potential tool for its differential diagnosis. Further studies with larger cohorts are required to confirm the relationship between CC and conventional OSCC, and to fully elucidate the genetic landscape of CC.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1007\/s12105-026-01921-3","type":"journal-article","created":{"date-parts":[[2026,5,25]],"date-time":"2026-05-25T05:13:51Z","timestamp":1779686031000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Genetic Landscape of Oral Carcinoma Cuniculatum and its Histological Mimics"],"prefix":"10.1007","volume":"20","author":[{"given":"Sawako","family":"Ono","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuki","family":"Fukawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4148-1106","authenticated-orcid":false,"given":"Katsutoshi","family":"Hirose","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yumiko","family":"Hori","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daisuke","family":"Motooka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroyuki","family":"Harada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eiichi","family":"Morii","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satoru","family":"Toyosawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naozumi","family":"Ishimaru","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hidetaka","family":"Yamamoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,5,25]]},"reference":[{"key":"1921_CR1","volume-title":"WHO Classification of Tumours, Head and Neck Tumours","author":"The WHO classification of tumours editorial board","year":"2024","unstructured":"The WHO classification of tumours editorial board (2024) WHO Classification of Tumours, Head and Neck Tumours, 5th edn. 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