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When donor chimerism is in the micro range (&lt;\u20091%), deviations in the accuracy of assessment are the most significant disadvantage of these common methods. We developed a highly sensitive method that applies SNPs based on NGS in order to explore the value of donor cell microchimerism in microtransplantation (MST). This improved SNP-NGS approach has higher sensitivity (0.01\u20130.05%) and only requires a small amount of DNA (8\u2013200\u00a0ng). We retrospectively analyzed the clinical data of 48 patients with AML who received HLA-mismatched stem cell MST at our center to assess the impact of microchimerism on clinical prognosis. Patients whose duration of microchimerism was\u2009&gt;\u200910.5\u00a0months (median) had a relapse rate of 26.1%, and had better 5-year LFS and OS (73.4% and 82.6%). In contrast, patients whose duration of microchimerism was\u2009&lt;\u200910.5\u00a0months had a higher relapse rate (69.6%), and their 5-year LFS and OS were 30.4% and 43.5%. In conclusion, duration of donor chimerism is highly valuable for assessment of survival and prognosis in patients with AML who have received HLA-mismatched stem cell MST, especially the intermediate-risk group.<\/jats:p>","DOI":"10.1007\/s12185-022-03415-8","type":"journal-article","created":{"date-parts":[[2022,7,8]],"date-time":"2022-07-08T08:13:19Z","timestamp":1657267999000},"page":"723-730","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["The clinical application of SNP-based next-generation sequencing (SNP-NGS) for evaluation of chimerism and microchimerism after HLA-mismatched stem cell microtransplantation"],"prefix":"10.1007","volume":"116","author":[{"given":"Weiyang","family":"Li","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi","family":"Xu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yufeng","family":"Feng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haixia","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao","family":"Ma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Depei","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suning","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aining","family":"Sun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2022,7,8]]},"reference":[{"issue":"12","key":"3415_CR1","doi-asserted-by":"publisher","first-page":"1038","DOI":"10.1016\/j.jcma.2018.06.005","volume":"81","author":"CT Chen","year":"2018","unstructured":"Chen CT, Gau JP, Liu JH, Chiou TJ, Hsiao LT, Liu YC. Early achievement of full donor chimerism after allogeneic hematopoietic stem cell transplantation predicts lower relapse risk in patients with acute lymphoblastic leukemia. J Chin Med Assoc. 2018;81(12):1038\u201343. https:\/\/doi.org\/10.1016\/j.jcma.2018.06.005.","journal-title":"J Chin Med Assoc"},{"issue":"5","key":"3415_CR2","doi-asserted-by":"publisher","first-page":"267","DOI":"10.1111\/tan.13005","volume":"89","author":"HJ Kolb","year":"2017","unstructured":"Kolb HJ. Hematopoietic stem cell transplantation and cellular therapy. HLA. 2017;89(5):267\u201377. https:\/\/doi.org\/10.1111\/tan.13005.","journal-title":"HLA"},{"issue":"11","key":"3415_CR3","doi-asserted-by":"publisher","first-page":"1285","DOI":"10.1016\/j.leukres.2014.08.018","volume":"38","author":"X Sun","year":"2014","unstructured":"Sun X, Guo M, Sun Q, Li B, Sun Y, Yao B, et al. The existence and role of microchimerism after microtransplantion. 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All authors have read and approved the content, and agree to submit for consideration for publication in the journal. There are no any ethical\/legal conflicts involved in the article.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}}]}}