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We describe genotypic and phenotypic characterization of 62 non-duplicate MRSA isolates collected during 2010\u20132016 at University Hospital in Hradec Kralove, Czech Republic. The isolates were characterized by multilocus sequence typing (MLST), <jats:italic>spa<\/jats:italic> typing, and staphylococcal cassette chromosome <jats:italic>mec<\/jats:italic> typing (SCC<jats:italic>mec<\/jats:italic> typing). Eight different genotypes were described; ST225-t003-II (32\/62, 52%), ST5-t002-II (13\/62, 22%), and ST225-t014-II (12\/62, 21%) were constantly detected over the 7-year follow-up period. The genotypes ST225-t151-II, ST225-t1282-II, ST225-t1623-II, ST78-t2832-II, and ST225-t8799-II occurred only once in the period reported. The majority of the strains, represented by ST225, belonged to clonal complex 5 (CC5).<\/jats:p>","DOI":"10.1007\/s12223-020-00782-9","type":"journal-article","created":{"date-parts":[[2020,3,14]],"date-time":"2020-03-14T06:03:06Z","timestamp":1584165786000},"page":"615-622","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Epidemiological characteristics of methicillin-resistant Staphylococcus aureus isolates from bloodstream cultures at University Hospital in the Czech Republic"],"prefix":"10.1007","volume":"65","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5239-146X","authenticated-orcid":false,"given":"Katerina","family":"Neradova","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marta","family":"Fridrichova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vladislav","family":"Jakubu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katarina","family":"Pomorska","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3868-0545","authenticated-orcid":false,"given":"Helena","family":"Zemlickova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2020,3,14]]},"reference":[{"key":"782_CR1","unstructured":"European Centre for Disease Prevention and Control Antimicrobial resistance surveillance in Europe (2016) Annual Report of the European Antimicrobial Resistance Surveillance Network (EARS-Net) 2016. https:\/\/www.ecdc.europa.eu\/en\/publications-data\/antimicrobial-resistancesurveillance-europe-2016. 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