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For that, the <jats:italic>Brucellae<\/jats:italic> produce catalase and superoxide dismutase to defend against oxidative stress. Since the deletion of the <jats:italic>mglA<\/jats:italic> gene in the <jats:italic>B. abortus S19<\/jats:italic> vaccine strain resulted not only in an increased tolerance to H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> but also in the induction of cytokines in macrophages, we here investigated the effect of oxidative stress (Fe<jats:sup>2+<\/jats:sup> and H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>) on the survival of <jats:italic>B. abortus S19<\/jats:italic> and the isogenic <jats:italic>B. abortus S 19 \u2206mglA 3.14<\/jats:italic> deletion mutant in comparison with <jats:italic>B. neotomae 5K33<\/jats:italic>, <jats:italic>Brucella strain 83\/13<\/jats:italic>, and <jats:italic>B. microti CCM4915.<\/jats:italic> These <jats:italic>Brucellae<\/jats:italic> belong to different phylogenetic clades and show characteristic differences in the mgl-operon. From the various <jats:italic>Brucellae<\/jats:italic> tested, <jats:italic>B. abortus S19<\/jats:italic> showed the highest susceptibility to oxidative stress and the lowest ability to survive inside of murine macrophages. <jats:italic>B. abortus S19 \u2206mglA 3.14<\/jats:italic> as well as <jats:italic>B. neotomae<\/jats:italic>, which also belongs to the classical core clade of <jats:italic>Brucella<\/jats:italic> and lacks the regulators of the mgl-operon, presented the highest degree of tolerance to H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> but not in the survival in macrophages. The latter was most pronounced in case of an infection with <jats:italic>B. 83\/13<\/jats:italic> and <jats:italic>B. microti CCM4915.<\/jats:italic> The various <jats:italic>Brucellae<\/jats:italic> investigated here demonstrate significant differences in tolerance against oxidative stress and different survival in murine macrophages, which, however, do not correlate directly.<\/jats:p>","DOI":"10.1007\/s12223-020-00798-1","type":"journal-article","created":{"date-parts":[[2020,5,28]],"date-time":"2020-05-28T00:02:15Z","timestamp":1590624135000},"page":"879-894","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Survival of Brucella abortus S19 and other Brucella spp. in the presence of oxidative stress and within macrophages"],"prefix":"10.1007","volume":"65","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7039-0735","authenticated-orcid":false,"given":"Jens","family":"Jacob","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antje","family":"Finke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Mielke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,5,28]]},"reference":[{"key":"798_CR1","doi-asserted-by":"publisher","first-page":"352","DOI":"10.1186\/1471-2164-10-352","volume":"10","author":"S Audic","year":"2009","unstructured":"Audic S, Lescot M, Claverie JM, Scholz HC (2009) Brucella microti: the genome sequence of an emerging pathogen. 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