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In the present study, five <jats:italic>T<\/jats:italic>. <jats:italic>bernardiae<\/jats:italic> isolates, isolated from five Peking ducks of four different farms, were identified by phenotypic properties, by matrix-assisted laser desorption\/ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis, and genotypically by sequencing the 16S ribosomal RNA (rRNA) gene, the superoxide dismutase A encoding gene <jats:italic>sodA<\/jats:italic>, and the glyceraldehyde-3-phosphate dehydrogenase encoding gene <jats:italic>gap<\/jats:italic>. In addition, the <jats:italic>T<\/jats:italic>. <jats:italic>bernardiae<\/jats:italic> isolates could be identified with a newly developed loop-mediated isothermal amplification (LAMP) assay based on the gyrase encoding housekeeping gene <jats:italic>gyrA<\/jats:italic>. All these tests clearly identified the <jats:italic>T<\/jats:italic>. <jats:italic>bernardiae<\/jats:italic> isolates to the species level. However, the detection of the specific gene <jats:italic>gyrA<\/jats:italic> with the newly designed LAMP assay appeared with a high sensitivity and specificity, and could help to identify this bacterial species in human and animal infections in future. The importance of the <jats:italic>T<\/jats:italic>. <jats:italic>bernardiae<\/jats:italic> isolates for the clinical condition of the ducks and for the problems at farm level remains unclear.<\/jats:p>","DOI":"10.1007\/s12223-021-00927-4","type":"journal-article","created":{"date-parts":[[2021,11,15]],"date-time":"2021-11-15T15:02:57Z","timestamp":1636988577000},"page":"277-284","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Identification of Trueperella bernardiae isolated from peking ducks (Anas platyrhynchos domesticus) by phenotypical and genotypical investigations and by a newly developed loop-mediated isothermal amplification (LAMP) assay"],"prefix":"10.1007","volume":"67","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5513-0206","authenticated-orcid":false,"given":"Marwa F. 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