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In the current situation, phage-based biocontrol strategies offer a promising alternative, leveraging their high specificity and efficacy against multidrug-resistant bacteria. The present study reports the phenotypic and genotypic characterizations of three broad-host-range MRSA phages: \u03c6CIFT_MFB_MRSA12, \u03c6CIFT_MFB_MRSA28, and \u03c6CIFT_MFB_MRSA32 for their application in seafood safety. The phages exhibited burst sizes ranging from 75 to 107 PFU\/cell and burst periods of 80\u201390\u00a0min. The thermal and pH stability studies indicated that \u03c6CIFT_MFB_MRSA12 exhibited the highest thermal stability (\u2212\u200920 to 60\u00a0\u00b0C), while \u03c6CIFT_MFB_MRSA28 demonstrated the widest pH tolerance (pH 3\u201312). The genomic analysis indicated that the phages possessed linear double-stranded DNA ranging from 141,193 to 141,505\u00a0bp, with large direct terminal repeats (DTRs) of 10,893\u00a0bp and various coding and non-coding genes (group-I introns, HEARO, and RAGATH). The comparative genome analysis revealed that three phages were found to be closely related to\n                    <jats:italic>Silviavirus<\/jats:italic>\n                    phages of the Herelleviridae family and differed with respect to tail fiber proteins, Ig domain-like carbohydrate-binding domains, and certain hypothetical proteins. Interestingly, the intergenomic and phylogenetic analyses revealed that the phages belonged to a novel species. Importantly, the genomes lacked virulence factors, antimicrobial resistance genes, or lysogenic determinants, supporting their safety in biocontrol strategies. The three\n                    <jats:italic>Silviavirus<\/jats:italic>\n                    phages could be potential candidates for the biocontrol of MRSA in seafood supply chains, thereby contributing to food safety and security.\n                  <\/jats:p>","DOI":"10.1007\/s12223-025-01290-4","type":"journal-article","created":{"date-parts":[[2025,7,10]],"date-time":"2025-07-10T09:14:37Z","timestamp":1752138877000},"page":"701-718","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Novel Silviavirus phages with broad-host-range activity against methicillin resistant Staphylococcus aureus from seafood: comprehending the phenotypic and genotypic variability"],"prefix":"10.1007","volume":"71","author":[{"given":"Karthika","family":"Raveendran","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sifana","family":"Sharaf","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ammu Lakshmi","family":"D.","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reshmi","family":"K.","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toms Cheriyath","family":"Joseph","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raja Swaminathan","family":"Thangaraj","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Visnuvinayagam","family":"Sivam","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shashikanta","family":"Parida","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nagendra R.","family":"Hegde","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ravindranath","family":"Shashidhar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Madhusudana Rao","family":"Badireddy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vandan","family":"Nagar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5807-9290","authenticated-orcid":false,"given":"Murugadas","family":"Vaiyapuri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,7,8]]},"reference":[{"key":"1290_CR1","doi-asserted-by":"publisher","first-page":"70","DOI":"10.3390\/v9040070","volume":"9","author":"E Adriaenssens","year":"2017","unstructured":"Adriaenssens E, Brister J (2017) How to name and classify your phage: an informal guide. 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