{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T20:51:30Z","timestamp":1793047890072,"version":"4.5.0"},"reference-count":37,"publisher":"Springer Science and Business Media LLC","issue":"4","license":[{"start":{"date-parts":[[2025,7,28]],"date-time":"2025-07-28T00:00:00Z","timestamp":1753660800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2025,7,28]],"date-time":"2025-07-28T00:00:00Z","timestamp":1753660800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100001824","name":"Grantov\u00e1 Agentura \u010cesk\u00e9 Republiky","doi-asserted-by":"publisher","award":["23-05154S"],"award-info":[{"award-number":["23-05154S"]}],"id":[{"id":"10.13039\/501100001824","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100016367","name":"Vysok\u00e1 \u0160kola Chemicko-technologick\u00e1 v Praze","doi-asserted-by":"publisher","award":["A2_FPBT_2024_023"],"award-info":[{"award-number":["A2_FPBT_2024_023"]}],"id":[{"id":"10.13039\/501100016367","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100016367","name":"University of Chemistry and Technology, Prague","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100016367","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Folia Microbiol"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Nanofibrous materials (NMs), widely used in medical and food industry applications, are highly susceptible to colonisation by microorganisms, including yeasts. Although yeasts can form dense biofilms, methods for studying their metabolic activity remain limited. This study is the first to apply the MTT assay, a standardised method for assessing cell metabolic activity, to evaluate the metabolic activity of\u00a0yeast biofilm-forming cells on electrospun NMs. First, the biofilm formation of\n                    <jats:italic>Candida albicans<\/jats:italic>\n                    and\n                    <jats:italic>Saccharomyces cerevisiae<\/jats:italic>\n                    on NMs electrospun from polycaprolactone (PCL), polylactic acid (PLA), and polyamide (PA) was examined. Then, key parameters of the MTT assay were systematically evaluated: (i) the addition of glucose to the MTT solution, (ii) the presence of menadione in the MTT solution, and (iii) the incubation time with the MTT solution. The addition of glucose was not proven necessary; however, in some cases, it may help distinguish the number of metabolically active cells. Based on this study, we recommend incubation with an MTT solution containing menadione for 2\u00a0h. To verify the protocol, colony-forming unit (CFU) enumeration was employed as a reference method. As differences between the results of these two methods were observed, the MTT assay should be complemented by other standardised methods. Nevertheless, the refined protocol offers a solid basis for investigating interactions between NMs and yeasts.\n                  <\/jats:p>","DOI":"10.1007\/s12223-025-01304-1","type":"journal-article","created":{"date-parts":[[2025,7,28]],"date-time":"2025-07-28T22:58:54Z","timestamp":1753743534000},"page":"899-908","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Assessing metabolic activity of yeast biofilm-forming cells on nanofibrous materials using MTT assay"],"prefix":"10.1007","volume":"71","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5085-8983","authenticated-orcid":false,"given":"Marta","family":"Stindlova","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0597-1063","authenticated-orcid":false,"given":"Vaclav","family":"Peroutka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8170-3263","authenticated-orcid":false,"given":"Kamila","family":"Zdenkova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6874-5944","authenticated-orcid":false,"given":"Simona","family":"Lencova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,7,28]]},"reference":[{"key":"1304_CR1","doi-asserted-by":"publisher","unstructured":"Alonso VPP, Lemos JG, da Silva do Nascimento M (2023) Yeast biofilms on abiotic surfaces: Adhesion factors and control methods. Int J Food Microbiol 400:110265. https:\/\/doi.org\/10.1016\/j.ijfoodmicro.2023.110265","DOI":"10.1016\/j.ijfoodmicro.2023.110265"},{"issue":"9","key":"1304_CR2","doi-asserted-by":"publisher","first-page":"2601","DOI":"10.1080\/10408398.2022.2124506","volume":"64","author":"M Aman Mohammadi","year":"2024","unstructured":"Aman Mohammadi M, Dakhili S, Mirza Alizadeh A, Kooki S, Hassanzadazar H, Alizadeh-Sani M, McClements DJ (2024) New perspectives on electrospun nanofiber applications in smart and active food packaging materials. Crit Rev Food Sci Nutr 64(9):2601\u20132617. https:\/\/doi.org\/10.1080\/10408398.2022.2124506","journal-title":"Crit Rev Food Sci Nutr"},{"issue":"9","key":"1304_CR3","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0162465","volume":"11","author":"MA Ansari","year":"2016","unstructured":"Ansari MA, Fatima Z, Hameed S (2016) Anticandidal Effect and Mechanisms of Monoterpenoid, Perillyl Alcohol against Candida albicans. PLoS ONE 11(9):e0162465. https:\/\/doi.org\/10.1371\/journal.pone.0162465","journal-title":"PLoS ONE"},{"issue":"4","key":"1304_CR4","doi-asserted-by":"publisher","first-page":"e106","DOI":"10.14440\/jbm.2018.272","volume":"5","author":"ZR Belak","year":"2018","unstructured":"Belak ZR, Harkness T, Eskiw CH (2018) A rapid, high-throughput method for determining chronological lifespan in budding yeast. J Biol Methods 5(4):e106. https:\/\/doi.org\/10.14440\/jbm.2018.272","journal-title":"J Biol Methods"},{"issue":"2","key":"1304_CR5","doi-asserted-by":"publisher","first-page":"474","DOI":"10.1006\/abbi.1993.1311","volume":"303","author":"MV Berridge","year":"1993","unstructured":"Berridge MV, Tan AS (1993) Characterization of the Cellular Reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT): Subcellular Localization, Substrate Dependence, and Involvement of Mitochondrial Electron Transport in MTT Reduction. Arch Biochem Biophys 303(2):474\u2013482. https:\/\/doi.org\/10.1006\/abbi.1993.1311","journal-title":"Arch Biochem Biophys"},{"key":"1304_CR6","doi-asserted-by":"publisher","first-page":"127","DOI":"10.1016\/s1387-2656(05)11004-7","volume":"11","author":"MV Berridge","year":"2005","unstructured":"Berridge MV, Herst PM, Tan AS (2005) Tetrazolium dyes as tools in cell biology: new insights into their cellular reduction. Biotechnol Annu Rev 11:127\u2013152. https:\/\/doi.org\/10.1016\/s1387-2656(05)11004-7","journal-title":"Biotechnol Annu Rev"},{"issue":"1","key":"1304_CR7","doi-asserted-by":"publisher","DOI":"10.1088\/1468-6996\/11\/1\/014108","volume":"11","author":"W Cui","year":"2010","unstructured":"Cui W, Zhou Y, Chang J (2010) Electrospun nanofibrous materials for tissue engineering and drug delivery. Sci Technol Adv Mater 11(1):014108. https:\/\/doi.org\/10.1088\/1468-6996\/11\/1\/014108","journal-title":"Sci Technol Adv Mater"},{"key":"1304_CR8","doi-asserted-by":"publisher","unstructured":"Dogan O, Atac N, Babuccu G, Can F (2021) Comparison of crystal violet staining assay and XTT methods in the evaluation of biofilm formation in Candida parapsilosis Candidemia isolates. Infect Dis Clin Micr 3:158\u2013163. https:\/\/doi.org\/10.36519\/idcm.2021.104","DOI":"10.36519\/idcm.2021.104"},{"issue":"3\u20134","key":"1304_CR9","doi-asserted-by":"publisher","first-page":"265","DOI":"10.1007\/s11046-013-9638-z","volume":"175","author":"AV Ferreira","year":"2013","unstructured":"Ferreira AV, Prado CG, Carvalho RR, Dias KS, Dias AL (2013) Candida albicans and non-C. albicans Candida species: comparison of biofilm production and metabolic activity in biofilms, and putative virulence properties of isolates from hospital environments and infections. Mycopathologia 175(3\u20134):265\u2013272. https:\/\/doi.org\/10.1007\/s11046-013-9638-z","journal-title":"Mycopathologia"},{"issue":"3","key":"1304_CR10","doi-asserted-by":"publisher","first-page":"524","DOI":"10.3390\/macromol3030031","volume":"3","author":"GG Flores-Rojas","year":"2023","unstructured":"Flores-Rojas GG, G\u00f3mez-Lazaro B, L\u00f3pez-Saucedo F, Vera-Graziano R, Bucio E, Mendiz\u00e1bal E (2023) Electrospun scaffolds for tissue engineering: a review. Macromol 3(3):524\u2013553.\u00a0https:\/\/doi.org\/10.3390\/macromol3030031","journal-title":"Macromol"},{"issue":"2","key":"1304_CR11","doi-asserted-by":"publisher","first-page":"253","DOI":"10.1016\/0022-1759(94)90062-0","volume":"168","author":"H Garn","year":"1994","unstructured":"Garn H, Krause H, Enzmann V, Dr\u00f6\u03b2ler K (1994) An improved MTT assay using the electron-coupling agent menadione. J Immunol Methods 168(2):253\u2013256. https:\/\/doi.org\/10.1016\/0022-1759(94)90062-0","journal-title":"J Immunol Methods"},{"key":"1304_CR12","doi-asserted-by":"crossref","unstructured":"Ghasemi M, Turnbull T, Sebastian S, Kempson I (2021) The MTT Assay: utility, limitations, pitfalls, and interpretation in bulk and single-cell analysis. Int J Mol Sci 22(23):12827. https:\/\/doi.org\/10.3390\/ijms222312827","DOI":"10.3390\/ijms222312827"},{"issue":"19","key":"1304_CR13","doi-asserted-by":"publisher","first-page":"3271","DOI":"10.1080\/10408398.2019.1682965","volume":"60","author":"B Hosnedlova","year":"2020","unstructured":"Hosnedlova B, Sochor J, Baron M, Bj\u00f8rklund G, Kizek R (2020) Application of nanotechnology based-biosensors in analysis of wine compounds and control of wine quality and safety: a critical review. Crit Rev Food Sci Nutr 60(19):3271\u20133289. https:\/\/doi.org\/10.1080\/10408398.2019.1682965","journal-title":"Crit Rev Food Sci Nutr"},{"issue":"11","key":"1304_CR14","doi-asserted-by":"publisher","first-page":"3198","DOI":"10.1021\/acs.jafc.8b05024","volume":"67","author":"M-X Hu","year":"2019","unstructured":"Hu M-X, Li J-N, Guo Q, Zhu Y-Q, Niu H-M (2019) Probiotics biofilm-integrated electrospun nanofiber membranes: a new starter culture for fermented milk production. J Agric Food Chem 67(11):3198\u20133208. https:\/\/doi.org\/10.1021\/acs.jafc.8b05024","journal-title":"J Agric Food Chem"},{"issue":"11","key":"1304_CR15","doi-asserted-by":"publisher","DOI":"10.1016\/j.fbio.2023.103394","volume":"56","author":"MX Hu","year":"2023","unstructured":"Hu MX, He F, Tu CK, Chen ZX, Teng H, Shao X, Ren GR, Guo YX (2023) Edible electrospun zein nanofibrous scaffolds close the gaps in biofilm formation ability between microorganisms. Food Biosci 56(11):103394. https:\/\/doi.org\/10.1016\/j.fbio.2023.103394","journal-title":"Food Biosci"},{"issue":"2","key":"1304_CR16","doi-asserted-by":"publisher","first-page":"123","DOI":"10.1080\/mmy.40.2.123.129","volume":"40","author":"C Imbert","year":"2002","unstructured":"Imbert C, Rodier M-H, Daniault G, Jacquemin J-L (2002) Influence of sub-inhibitory concentrations of conventional antifungals on metabolism of Candida albicans and on its adherence to polystyrene and extracellular matrix proteins. Med Mycol 40(2):123\u2013129. https:\/\/doi.org\/10.1080\/mmy.40.2.123.129","journal-title":"Med Mycol"},{"issue":"11","key":"1304_CR17","doi-asserted-by":"publisher","first-page":"2391","DOI":"10.3390\/microorganisms9112391","volume":"9","author":"Z Kode\u0161","year":"2021","unstructured":"Kode\u0161 Z, Vrublevskaya M, Kuli\u0161ov\u00e1 M, Jaro\u0161 P, Paldrychov\u00e1 M, P\u00e1drov\u00e1 K, Loko\u010dov\u00e1 K, Palyzov\u00e1 A, Ma\u0165\u00e1tkov\u00e1 O, Kolouchov\u00e1 I (2021) Composition and biological activity of Vitis vinifera winter cane extract on Candida Biofilm. Microorganisms 9(11):2391. https:\/\/doi.org\/10.3390\/microorganisms9112391","journal-title":"Microorganisms"},{"issue":"2","key":"1304_CR18","doi-asserted-by":"publisher","first-page":"2277","DOI":"10.1021\/acsami.0c19016","volume":"13","author":"S Lencova","year":"2021","unstructured":"Lencova S, Svarcova V, Stiborova H, Demnerova K, Jencova V, Hozdova K, Zdenkova K (2021) Bacterial biofilms on polyamide nanofibers: factors influencing biofilm formation and evaluation. ACS Appl Mater Interfaces 13(2):2277\u20132288. https:\/\/doi.org\/10.1021\/acsami.0c19016","journal-title":"ACS Appl Mater Interfaces"},{"issue":"20","key":"1304_CR19","doi-asserted-by":"publisher","first-page":"25813","DOI":"10.1021\/acsami.4c03642","volume":"16","author":"S Lencova","year":"2024","unstructured":"Lencova S, Stindlova M, Havlickova K, Jencova V, Peroutka V, Navratilova K, Zdenkova K, Stiborova H, Hauzerova S, Kostakova EK, Jankovsky O, Kejzlar P, Lukas D, Demnerova K (2024) Influence of fiber diameter of polycaprolactone nanofibrous materials on biofilm formation and retention of bacterial cells. ACS Appl Mater Interfaces 16(20):25813\u201325824. https:\/\/doi.org\/10.1021\/acsami.4c03642","journal-title":"ACS Appl Mater Interfaces"},{"issue":"5","key":"1304_CR20","doi-asserted-by":"publisher","first-page":"938","DOI":"10.1093\/infdis\/152.5.938","volume":"152","author":"SM Levitz","year":"1985","unstructured":"Levitz SM, Diamond RD (1985) A rapid colorimetric assay of fungal viability with the tetrazolium salt MTT. J Infect Dis 152(5):938\u2013945. https:\/\/doi.org\/10.1093\/infdis\/152.5.938","journal-title":"J Infect Dis"},{"issue":"1","key":"1304_CR21","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1038\/nrmicro.2017.107","volume":"16","author":"MB Lohse","year":"2018","unstructured":"Lohse MB, Gulati M, Johnson AD, Nobile CJ (2018) Development and regulation of single- and multi-species Candida albicans biofilms. Nat Rev Microbiol 16(1):19\u201331. https:\/\/doi.org\/10.1038\/nrmicro.2017.107","journal-title":"Nat Rev Microbiol"},{"issue":"1","key":"1304_CR22","doi-asserted-by":"publisher","first-page":"14966","DOI":"10.1038\/s41598-019-51241-6","volume":"9","author":"M Mazloum-Ardakani","year":"2019","unstructured":"Mazloum-Ardakani M, Barazesh B, Moshtaghioun SM, Sheikhha MH (2019) Designing and optimization of an electrochemical substitute for the MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) cell viability assay. Sci Rep 9(1):14966. https:\/\/doi.org\/10.1038\/s41598-019-51241-6","journal-title":"Sci Rep"},{"key":"1304_CR23","doi-asserted-by":"publisher","DOI":"10.1016\/j.msec.2020.111450","volume":"119","author":"P Mishra","year":"2021","unstructured":"Mishra P, Gupta P, Pruthi V (2021) Cinnamaldehyde incorporated gellan\/PVA electrospun nanofibers for eradicating Candida biofilm. Mater Sci Eng C 119:111450. https:\/\/doi.org\/10.1016\/j.msec.2020.111450","journal-title":"Mater Sci Eng C"},{"key":"1304_CR24","doi-asserted-by":"publisher","DOI":"10.1016\/j.nantod.2022.101704","volume":"48","author":"A Morita","year":"2023","unstructured":"Morita A, Nusantara AC, Myzk A, Perona Martinez FP, Hamoh T, Damle VG, van der Laan KJ, Sigaeva A, Vedelaar T, Chang M, Chipaux M, Schirhagl R (2023) Detecting the metabolism of individual yeast mutant strain cells when aged, stressed or treated with antioxidants with diamond magnetometry. Nano Today 48:101704. https:\/\/doi.org\/10.1016\/j.nantod.2022.101704","journal-title":"Nano Today"},{"key":"1304_CR25","doi-asserted-by":"publisher","unstructured":"Nayl AA, Abd-Elhamid AI, Awwad NS, Abdelgawad MA, Wu J, Mo X, Gomha SM, Aly AA, Br\u00e4se S (2022) Recent progress and potential biomedical applications of electrospun nanofibers in regeneration of tissues and organs. Polymers-basel 14(8):1508. https:\/\/doi.org\/10.3390\/polym14081508","DOI":"10.3390\/polym14081508"},{"issue":"14","key":"1304_CR26","doi-asserted-by":"publisher","first-page":"11467","DOI":"10.1021\/acsami.7b19440","volume":"10","author":"C Rumbo","year":"2018","unstructured":"Rumbo C, Tamayo-Ramos JA, Caso MF, Rinaldi A, Romero-Santacreu L, Quesada R, Cuesta-L\u00f3pez S (2018) Colonization of electrospun polycaprolactone fibers by relevant pathogenic bacterial strains. ACS Appl Mater Interfaces 10(14):11467\u201311473. https:\/\/doi.org\/10.1021\/acsami.7b19440","journal-title":"ACS Appl Mater Interfaces"},{"issue":"4","key":"1304_CR27","doi-asserted-by":"publisher","first-page":"511","DOI":"10.1007\/s10529-019-02660-y","volume":"41","author":"G Sahal","year":"2019","unstructured":"Sahal G, Nasseri B, Ebrahimi A, Bilkay IS (2019) Electrospun essential oil-polycaprolactone nanofibers as antibiofilm surfaces against clinical Candida tropicalis isolates. Biotechnol Lett 41(4):511\u2013522. https:\/\/doi.org\/10.1007\/s10529-019-02660-y","journal-title":"Biotechnol Lett"},{"issue":"10","key":"1304_CR28","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0077600","volume":"8","author":"M Salma","year":"2013","unstructured":"Salma M, Rousseaux S, Sequeira-Le Grand A, Divol B, Alexandre H (2013) Characterization of the viable but nonculturable (VBNC) State in Saccharomyces cerevisiae. PLoS ONE 8(10):e77600. https:\/\/doi.org\/10.1371\/journal.pone.0077600","journal-title":"PLoS ONE"},{"issue":"2","key":"1304_CR29","doi-asserted-by":"publisher","first-page":"193","DOI":"10.1016\/j.gene.2015.08.009","volume":"574","author":"AA Stepanenko","year":"2015","unstructured":"Stepanenko AA, Dmitrenko VV (2015) Pitfalls of the MTT assay: Direct and off-target effects of inhibitors can result in over\/underestimation of cell viability. Gene 574(2):193\u2013203. https:\/\/doi.org\/10.1016\/j.gene.2015.08.009","journal-title":"Gene"},{"key":"1304_CR30","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2024.107010","volume":"224","author":"M Stindlova","year":"2024","unstructured":"Stindlova M, Peroutka V, Jencova V, Havlickova K, Lencova S (2024) Application of MTT assay for probing metabolic activity in bacterial biofilm-forming cells on nanofibrous materials. J Microbiol Methods 224:107010. https:\/\/doi.org\/10.1016\/j.mimet.2024.107010","journal-title":"J Microbiol Methods"},{"issue":"10","key":"1304_CR31","doi-asserted-by":"publisher","first-page":"3145","DOI":"10.1099\/mic.0.27296-0","volume":"150","author":"R Tepari\u0107","year":"2004","unstructured":"Tepari\u0107 R, Stuparevi\u0107 I, Mr\u0161a V (2004) Increased mortality of Saccharomyces cerevisiae cell wall protein mutants. Microbiology 150(10):3145\u20133150.\u00a0https:\/\/doi.org\/10.1099\/mic.0.27296-0","journal-title":"Microbiology"},{"issue":"19","key":"1304_CR32","doi-asserted-by":"publisher","DOI":"10.3390\/ijms231911665","volume":"23","author":"L Vanderwaeren","year":"2022","unstructured":"Vanderwaeren L, Dok R, Voordeckers K, Nuyts S, Verstrepen KJ (2022) Saccharomyces cerevisiae as a model system for eukaryotic cell biology, from cell cycle control to DNA damage response. Int J Mol Sci 23(19):11665. https:\/\/doi.org\/10.3390\/ijms231911665","journal-title":"Int J Mol Sci"},{"key":"1304_CR33","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2024.110901","volume":"426","author":"Y Xiao","year":"2025","unstructured":"Xiao Y, Wang J, Sun P, Ding T, Li J, Deng Y (2025) Formation and resuscitation of viable but non-culturable (VBNC) yeast in the food industry: A review. Int J Food Microbiol 426:110901. https:\/\/doi.org\/10.1016\/j.ijfoodmicro.2024.110901","journal-title":"Int J Food Microbiol"},{"issue":"8","key":"1304_CR34","doi-asserted-by":"publisher","first-page":"479","DOI":"10.1002\/yea.1787","volume":"27","author":"X Xie","year":"2010","unstructured":"Xie X, Lipke PN (2010) On the evolution of fungal and yeast cell walls. Yeast 27(8):479\u2013488. https:\/\/doi.org\/10.1002\/yea.1787","journal-title":"Yeast"},{"issue":"18","key":"1304_CR35","doi-asserted-by":"publisher","first-page":"e01110","DOI":"10.1128\/AEM.01110-21","volume":"87","author":"M Xie","year":"2021","unstructured":"Xie M, Xu L, Zhang R, Zhou Y, Xiao Y, Su X, Shen C, Sun F, Hashmi MZ, Lin H, Chen J (2021) Viable but nonculturable state of yeast Candida sp. Strain LN1 induced by high phenol concentrations. Appl Environ Microbiol 87(18):e01110-01121. https:\/\/doi.org\/10.1128\/AEM.01110-21","journal-title":"Appl Environ Microbiol"},{"issue":"9","key":"1304_CR36","doi-asserted-by":"publisher","first-page":"134","DOI":"10.1007\/s11274-020-02911-5","volume":"36","author":"G Zara","year":"2020","unstructured":"Zara G, Budroni M, Mannazzu I, Fancello F, Zara S (2020) Yeast biofilm in food realms: occurrence and control. World J Microbiol Biotechnol 36(9):134. https:\/\/doi.org\/10.1007\/s11274-020-02911-5","journal-title":"World J Microbiol Biotechnol"},{"issue":"6","key":"1304_CR37","doi-asserted-by":"publisher","first-page":"2375","DOI":"10.1021\/pr300447k","volume":"12","author":"Z Zhu","year":"2013","unstructured":"Zhu Z, Wang H, Shang Q, Jiang Y, Cao Y, Chai Y (2013) Time course analysis of Candida albicans metabolites during biofilm development. J Proteome Res 12(6):2375\u20132385. https:\/\/doi.org\/10.1021\/pr300447k","journal-title":"J Proteome Res"}],"container-title":["Folia Microbiologica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s12223-025-01304-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s12223-025-01304-1","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s12223-025-01304-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T05:46:06Z","timestamp":1788414366000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s12223-025-01304-1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,28]]},"references-count":37,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2026,8]]}},"alternative-id":["1304"],"URL":"https:\/\/doi.org\/10.1007\/s12223-025-01304-1","relation":{},"ISSN":["0015-5632","1874-9356"],"issn-type":[{"value":"0015-5632","type":"print"},{"value":"1874-9356","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,28]]},"assertion":[{"value":"26 February 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"16 July 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"28 July 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing interests.","order":1,"name":"Ethics","label":"Competing interests","group":{"name":"EthicsHeading","label":"Declarations"}}]}}