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Sin."],"published-print":{"date-parts":[[2017,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Feasible and effective cell models for hepatitis B virus (HBV) infection are\n                required for investigating the complete lifecycle of this virus, including the early\n                steps of viral entry. Resistance to dimethyl sulfoxide\/polyethylene glycol\n                (DMSO\/PEG), hNTCP expression, and a differentiated state are the limiting factors\n                for successful HBV infection models. In the present study, we used a hepatoma cell\n                line (Huh7D<jats:sup>hNTCP<\/jats:sup>) to overcome these limiting factors so\n                that it exhibits excellent susceptibility to HBV infection. To achieve this goal,\n                different hepatoma cell lines were tested with 2.5% DMSO \/ 4% PEG8000, and one\n                resistant cell line (Huh7D) was used to construct a stable hNTCP-expressing cell\n                line (Huh7D<jats:sup>hNTCP<\/jats:sup>) using a recombinant lentivirus system.\n                Then, the morphological characteristics and differentiation molecular markers of\n                    Huh7D<jats:sup>hNTCP<\/jats:sup> cells with or without DMSO treatment were\n                characterized. Finally, the susceptibility of Huh7D<jats:sup>hNTCP<\/jats:sup>\n                cells to HBV infection was assessed. Our results showed that Huh7D cells were\n                resistant to 2.5% DMSO \/ 4% PEG8000, whereas the others were not.\n                    Huh7D<jats:sup>hNTCP<\/jats:sup> cells were established to express a high\n                level of hNTCP compared to liver extracts, and Huh7D<jats:sup>hNTCP<\/jats:sup>\n                cells rapidly transformed into a non-dividing, well-differentiated polarized\n                phenotype under DMSO treatment. Huh7D<jats:sup>hNTCP<\/jats:sup> cells fully\n                supported the entire lifecycle of HBV infection. This cell culture system will be\n                useful for the analysis of host-virus interactions, which should facilitate the\n                discovery of antiviral drugs and vaccines.<\/jats:p>","DOI":"10.1007\/s12250-017-3983-x","type":"journal-article","created":{"date-parts":[[2017,10,2]],"date-time":"2017-10-02T09:13:18Z","timestamp":1506935598000},"page":"465-475","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Productive HBV infection of well-differentiated,\n            hNTCP-expressing human hepatoma-derived (Huh7) cells"],"prefix":"10.1016","volume":"32","author":[{"given":"Ming","family":"Zhou","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaitao","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongxuan","family":"Yao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yifei","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rongjuan","family":"Pei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jizheng","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xue","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinwen","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6888-213X","authenticated-orcid":false,"given":"Chunchen","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","published-online":{"date-parts":[[2017,9,29]]},"reference":[{"key":"3983_CR1","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1007\/s12250-014-3408-z","volume":"29","author":"H Cao","year":"2014","unstructured":"Cao H, Zhu W, Han Q, Pei R, Chen X. 2014. 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