{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,20]],"date-time":"2026-03-20T04:56:10Z","timestamp":1773982570851,"version":"3.50.1"},"reference-count":24,"publisher":"Elsevier BV","issue":"5","license":[{"start":{"date-parts":[[2017,10,1]],"date-time":"2017-10-01T00:00:00Z","timestamp":1506816000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2017,10,30]],"date-time":"2017-10-30T00:00:00Z","timestamp":1509321600000},"content-version":"vor","delay-in-days":29,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Virol. Sin."],"published-print":{"date-parts":[[2017,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Varicella-zoster virus (VZV) is a neurotropic alphaherpesvirus that causes\n                chickenpox and shingles. ORF7 is an important virulence determinant of VZV in both\n                human skin and nerve tissues, however, its specific function and involved molecular\n                mechanism in VZV pathogenesis remain largely elusive. Previous yeast two-hybrid\n                studies on intraviral protein-protein interaction network in herpesviruses have\n                revealed that VZV ORF7 may interact with ORF53, which is a virtually unstudied but\n                essential viral protein. The aim of this study is to identify and characterize VZV\n                ORF53, and to investigate its relationship with ORF7. For this purpose, we prepared\n                monoclonal antibodies against ORF53 and, for the first time, characterized it as a\n                ~40 kDa viral protein predominantly localizing to the trans-Golgi network of the\n                infected host cell. Next, we further confirmed the interaction between ORF7 and\n                ORF53 by co-immunoprecipitation and co-localization studies in both\n                plasmid-transfected and VZV-infected cells. Moreover, interestingly, we found that\n                ORF53 lost its trans-Golgi network localization and became dispersed in the\n                cytoplasm of host cells infected with an ORF7-deleted recombinant VZV, and thus ORF7\n                seems to play a role in normal subcellular localization of ORF53. Collectively,\n                these results suggested that ORF7 and ORF53 may function as a complex during\n                infection, which may be implicated in VZV pathogenesis. <\/jats:p>","DOI":"10.1007\/s12250-017-4048-x","type":"journal-article","created":{"date-parts":[[2017,11,7]],"date-time":"2017-11-07T08:41:14Z","timestamp":1510044074000},"page":"387-395","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Varicella-zoster virus ORF7 interacts with ORF53 and plays a\n            role in its trans-Golgi network localization"],"prefix":"10.1016","volume":"32","author":[{"given":"Wei","family":"Wang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenkun","family":"Fu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dequan","family":"Pan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Linli","family":"Cai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianghui","family":"Ye","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jian","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Che","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuqiong","family":"Que","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ningshao","family":"Xia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6087-3175","authenticated-orcid":false,"given":"Hua","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1638-6214","authenticated-orcid":false,"given":"Tong","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","published-online":{"date-parts":[[2017,10,30]]},"reference":[{"issue":"pii","key":"4048_CR1","first-page":"e02196","volume":"91","author":"A Albecka","year":"2017","unstructured":"Albecka A, Owen DJ, Ivanova L, Brun J, Liman R, Davies L, Ahmed MF,\n                    Colaco S, Hollinshead M, Graham SC, Crump CM. 2017. Dual function of the\n                    pul7-pul51 tegument protein complex in herpes simplex virus 1 infection. J\n                    Virol, 91. pii: e02196\u201316.","journal-title":"J Virol"},{"key":"4048_CR2","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1083\/jcb.200912163","volume":"189","author":"AR Barr","year":"2010","unstructured":"Barr AR, Kilmartin JV, Gergely F. 2010. Cdk5rap2 functions in\n                    centrosome to spindle pole attachment and DNA damage response. J Cell Biol, 189:\n                    23\u201339.","journal-title":"J Cell Biol"},{"key":"4048_CR3","doi-asserted-by":"publisher","first-page":"7618","DOI":"10.1128\/JVI.00657-14","volume":"88","author":"AS Charles","year":"2014","unstructured":"Charles AS, Chouljenko VN, Jambunathan N, Subramanian R, Mottram P,\n                    Kousoulas KG. 2014. Phenylalanine residues at the carboxyl terminus of the\n                    herpes simplex virus 1 ul20 membrane protein regulate cytoplasmic virion\n                    envelopment and infectious virus production. J Virol, 88:\n                    7618\u20137627.","journal-title":"J Virol"},{"key":"4048_CR4","first-page":"2773","volume-title":"Fields virology","author":"JI Cohen","year":"2007","unstructured":"Cohen JI, Straus SE, Arvin AM. 2007. Fields virology, 5th edn.\n                    Philadelphia: Lippincott Williams & Wilkins, pp.\n                    2773\u20132818."},{"key":"4048_CR5","doi-asserted-by":"publisher","first-page":"2114","DOI":"10.1038\/mt.2011.143","volume":"19","author":"A Donsante","year":"2011","unstructured":"Donsante A, Yi L, Zerfas PM, Brinster LR, Sullivan P, Goldstein DS,\n                    Prohaska J, Centeno JA, Rushing E, Kaler SG. 2011. Atp7a gene addition to the\n                    choroid plexus results in long-term rescue of the lethal copper transport defect\n                    in a menkes disease mouse model. Mol Ther, 19: 2114\u20132123.","journal-title":"Mol Ther"},{"key":"4048_CR6","doi-asserted-by":"publisher","first-page":"1731","DOI":"10.1503\/cmaj.091711","volume":"182","author":"M Drolet","year":"2010","unstructured":"Drolet M, Brisson M, Schmader KE, Levin MJ, Johnson R, Oxman MN,\n                    Patrick D, Blanchette C, Mansi JA. 2010. The impact of herpes zoster and\n                    postherpetic neuralgia on health-related quality of life: A prospective study.\n                    CMAJ, 182: 1731\u20131736.","journal-title":"CMAJ"},{"key":"4048_CR7","doi-asserted-by":"publisher","first-page":"635","DOI":"10.1056\/NEJM200003023420906","volume":"342","author":"DH Gilden","year":"2000","unstructured":"Gilden DH, Kleinschmidt-DeMasters BK, LaGuardia JJ, Mahalingam R,\n                    Cohrs RJ. 2000. Neurologic complications of the reactivation of varicella-zoster\n                    virus. N Engl J Med, 342: 635\u2013645.","journal-title":"N Engl J Med"},{"key":"4048_CR8","doi-asserted-by":"publisher","first-page":"462","DOI":"10.1007\/s13365-012-0124-z","volume":"18","author":"S Grigoryan","year":"2012","unstructured":"Grigoryan S, Kinchington PR, Yang IH, Selariu A, Zhu H, Yee M,\n                    Goldstein RS. 2012. 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