{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T11:46:23Z","timestamp":1774352783194,"version":"3.50.1"},"reference-count":37,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2019,2,1]],"date-time":"2019-02-01T00:00:00Z","timestamp":1548979200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2019,2,1]],"date-time":"2019-02-01T00:00:00Z","timestamp":1548979200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Virol. Sin."],"published-print":{"date-parts":[[2019,2]]},"DOI":"10.1007\/s12250-019-00090-8","type":"journal-article","created":{"date-parts":[[2019,2,21]],"date-time":"2019-02-21T08:36:04Z","timestamp":1550738164000},"page":"88-96","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Electroporation-Mediated Immunization of a Candidate DNA Vaccine Expressing Dengue Virus Serotype 4 prM-E Antigen Confers Long-Term Protection in Mice"],"prefix":"10.1016","volume":"34","author":[{"given":"Ziyang","family":"Sheng","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kaihao","family":"Feng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Na","family":"Gao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ran","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peigang","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dongying","family":"Fan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2946-7371","authenticated-orcid":false,"given":"Jing","family":"An","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","published-online":{"date-parts":[[2019,2,21]]},"reference":[{"key":"90_CR1","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.jbiotec.2004.01.015","volume":"110","author":"S Babiuk","year":"2004","unstructured":"Babiuk S, Baca-Estrada ME, Foldvari M, Middleton DM, Rabussay D, Widera G, Babiuk LA (2004) Increased gene expression and inflammatory cell infiltration caused by electroporation are both important for improving the efficacy of DNA vaccines. J Biotechnol 110:1\u201310","journal-title":"J Biotechnol"},{"key":"90_CR2","doi-asserted-by":"publisher","first-page":"504","DOI":"10.1038\/nature12060","volume":"496","author":"S Bhatt","year":"2013","unstructured":"Bhatt S, Gething PW, Brady OJ, Messina JP, Farlow AW, Moyes CL, Drake JM, Brownstein JS, Hoen AG, Sankoh O, Myers MF, George DB, Jaenisch T, Wint GR, Simmons CP, Scott TW, Farrar JJ, Hay SI (2013) The global distribution and burden of dengue. Nature 496:504\u2013507","journal-title":"Nature"},{"key":"90_CR3","doi-asserted-by":"publisher","first-page":"e34602","DOI":"10.1371\/journal.pone.0034602","volume":"7","author":"H Chen","year":"2012","unstructured":"Chen H, Gao N, Fan D, Wu J, Zhu J, Li J, Wang J, Chen Y, An J (2012) Suppressive effects on the immune response and protective immunity to a JEV DNA vaccine by co-administration of a GM-CSF-expressing plasmid in mice. PLoS ONE 7:e34602","journal-title":"PLoS ONE"},{"key":"90_CR4","doi-asserted-by":"publisher","first-page":"41","DOI":"10.1016\/j.clim.2016.08.021","volume":"171","author":"H Chen","year":"2016","unstructured":"Chen H, Zheng X, Wang R, Gao N, Sheng Z, Fan D, Feng K, Liao X, An J (2016) Immunization with electroporation enhances the protective effect of a DNA vaccine candidate expressing prME antigen against dengue virus serotype 2 infection. Clin Immunol 171:41\u201349","journal-title":"Clin Immunol"},{"key":"90_CR5","doi-asserted-by":"publisher","first-page":"2705","DOI":"10.1016\/j.vaccine.2010.01.022","volume":"28","author":"DE Clements","year":"2010","unstructured":"Clements DE, Coller BA, Lieberman MM, Ogata S, Wang G, Harada KE, Putnak JR, Ivy JM, McDonell M, Bignami GS, Peters ID, Leung J, Weeks-Levy C, Nakano ET, Humphreys T (2010) Development of a recombinant tetravalent dengue virus vaccine: immunogenicity and efficacy studies in mice and monkeys. Vaccine 28:2705\u20132715","journal-title":"Vaccine"},{"key":"90_CR6","doi-asserted-by":"publisher","first-page":"808","DOI":"10.1016\/j.vaccine.2016.12.034","volume":"35","author":"Z Dai","year":"2017","unstructured":"Dai Z, Huang J, Lei X, Yan Y, Lu P, Zhang H, Lin W, Chen W, Ma J, Xie Q (2017) Efficacy of an autophagy-targeted DNA vaccine against avian leukosis virus subgroup. J. Vaccine 35:808\u2013813","journal-title":"J. Vaccine"},{"key":"90_CR7","doi-asserted-by":"publisher","first-page":"010312","DOI":"10.7189\/jogh.08.020312","volume":"8","author":"K Fatima","year":"2018","unstructured":"Fatima K, Syed NI (2018) Dengvaxia controversy: impact on vaccine hesitancy. J Glob Health 8:010312","journal-title":"J Glob Health"},{"key":"90_CR8","doi-asserted-by":"publisher","first-page":"15743","DOI":"10.1038\/ncomms15743","volume":"8","author":"BD Griffin","year":"2017","unstructured":"Griffin BD, Muthumani K, Warner BM, Majer A, Hagan M, Audet J, Stein DR, Ranadheera C, Racine T, De La Vega MA, Piret J, Kucas S, Tran KN, Frost KL, De Graff C, Soule G, Scharikow L, Scott J, McTavish G, Smid V, Park YK, Maslow JN, Sardesai NY, Kim JJ, Yao XJ, Bello A, Lindsay R, Boivin G, Booth SA, Kobasa D, Embury-Hyatt C, Safronetz D, Weiner DB, Kobinger GP (2017) DNA vaccination protects mice against Zika virus-induced damage to the testes. Nat Commun 8:15743","journal-title":"Nat Commun"},{"key":"90_CR9","doi-asserted-by":"publisher","first-page":"S7","DOI":"10.1038\/nrmicro2460","volume":"8","author":"MG Guzman","year":"2010","unstructured":"Guzman MG, Halstead SB, Artsob H, Buchy P, Farrar J, Gubler DJ, Hunsperger E, Kroeger A, Margolis HS, Martinez E, Nathan MB, Pelegrino JL, Simmons C, Yoksan S, Peeling RW (2010) Dengue: a continuing global threat. Nat Rev Microbiol 8:S7\u201316","journal-title":"Nat Rev Microbiol"},{"key":"90_CR10","doi-asserted-by":"publisher","first-page":"1195","DOI":"10.1056\/NEJMoa1506223","volume":"373","author":"SR Hadinegoro","year":"2015","unstructured":"Hadinegoro SR, Arredondo-Garcia JL, Capeding MR, Deseda C, Chotpitayasunondh T, Dietze R, Muhammad Ismail HI, Reynales H, Limkittikul K, Rivera-Medina DM, Tran HN, Bouckenooghe A, Chansinghakul D, Cortes M, Fanouillere K, Forrat R, Frago C, Gailhardou S, Jackson N, Noriega F, Plennevaux E, Wartel TA, Zambrano B, Saville M, Group C-TDVW (2015) Efficacy and long-term safety of a dengue vaccine in regions of endemic disease. N Engl J Med 373:1195\u20131206","journal-title":"N Engl J Med"},{"key":"90_CR11","doi-asserted-by":"publisher","first-page":"2014","DOI":"10.1093\/infdis\/jiw458","volume":"214","author":"SB Halstead","year":"2016","unstructured":"Halstead SB (2016) Dengue vaccine efficacy: not a zero sum game. J Infect Dis 214:2014","journal-title":"J Infect Dis"},{"key":"90_CR12","doi-asserted-by":"publisher","first-page":"109","DOI":"10.1006\/viro.1994.1013","volume":"198","author":"FX Heinz","year":"1994","unstructured":"Heinz FX, Stiasny K, Puschner-Auer G, Holzmann H, Allison SL, Mandl CW, Kunz C (1994) Structural changes and functional control of the tick-borne encephalitis virus glycoprotein E by the heterodimeric association with protein prM. Virology 198:109\u2013117","journal-title":"Virology"},{"key":"90_CR13","doi-asserted-by":"publisher","first-page":"440","DOI":"10.1016\/j.vaccine.2007.10.041","volume":"26","author":"LA Hirao","year":"2008","unstructured":"Hirao LA, Wu L, Khan AS, Satishchandran A, Draghia-Akli R, Weiner DB (2008) Intradermal\/subcutaneous immunization by electroporation improves plasmid vaccine delivery and potency in pigs and rhesus macaques. Vaccine 26:440\u2013448","journal-title":"Vaccine"},{"key":"90_CR14","doi-asserted-by":"publisher","first-page":"3469","DOI":"10.1016\/j.vaccine.2016.04.025","volume":"34","author":"T Huang","year":"2016","unstructured":"Huang T, Zhao K, Zhang Z, Tang C, Zhang X, Yue B (2016) DNA vaccination based on pyolysin co-immunized with IL-1beta enhances host antibacterial immunity against Trueperella pyogenes infection. Vaccine 34:3469\u20133477","journal-title":"Vaccine"},{"key":"90_CR15","doi-asserted-by":"publisher","first-page":"233","DOI":"10.1016\/j.coviro.2011.08.003","volume":"1","author":"NA Hutnick","year":"2011","unstructured":"Hutnick NA, Myles DJ, Bian CB, Muthumani K, Weiner DB (2011) Selected approaches for increasing HIV DNA vaccine immunogenicity in vivo. Curr Opin Virol 1:233\u2013240","journal-title":"Curr Opin Virol"},{"key":"90_CR16","doi-asserted-by":"publisher","first-page":"26","DOI":"10.11599\/germs.2013.1034","volume":"3","author":"KH Khan","year":"2013","unstructured":"Khan KH (2013) DNA vaccines: roles against diseases. Germs 3:26\u201335","journal-title":"Germs"},{"key":"90_CR17","doi-asserted-by":"publisher","first-page":"7297","DOI":"10.1016\/j.vaccine.2010.08.079","volume":"28","author":"D Kim","year":"2010","unstructured":"Kim D, Hung CF, Wu TC, Park YM (2010) DNA vaccine with alpha-galactosylceramide at prime phase enhances anti-tumor immunity after boosting with antigen-expressing dendritic cells. Vaccine 28:7297\u20137305","journal-title":"Vaccine"},{"key":"90_CR18","doi-asserted-by":"publisher","first-page":"295","DOI":"10.1517\/17425247.2016.1121990","volume":"13","author":"L Lambricht","year":"2016","unstructured":"Lambricht L, Lopes A, Kos S, Sersa G, Preat V, Vandermeulen G (2016) Clinical potential of electroporation for gene therapy and DNA vaccine delivery. Expert Opin Drug Deliv 13:295\u2013310","journal-title":"Expert Opin Drug Deliv"},{"key":"90_CR19","doi-asserted-by":"publisher","first-page":"62","DOI":"10.1111\/j.1600-065X.2010.00980.x","volume":"239","author":"MA Liu","year":"2011","unstructured":"Liu MA (2011) DNA vaccines: an historical perspective and view to the future. Immunol Rev 239:62\u201384","journal-title":"Immunol Rev"},{"key":"90_CR20","doi-asserted-by":"publisher","first-page":"1056","DOI":"10.1016\/j.vaccine.2009.10.111","volume":"28","author":"BD Livingston","year":"2010","unstructured":"Livingston BD, Little SF, Luxembourg A, Ellefsen B, Hannaman D (2010) Comparative performance of a licensed anthrax vaccine versus electroporation based delivery of a PA encoding DNA vaccine in rhesus macaques. Vaccine 28:1056\u20131061","journal-title":"Vaccine"},{"key":"90_CR21","doi-asserted-by":"publisher","first-page":"648","DOI":"10.1016\/S0264-410X(00)00247-4","volume":"19","author":"R Ocazionez Jimenez","year":"2000","unstructured":"Ocazionez Jimenez R, Lopes da Fonseca BA (2000) Recombinant plasmid expressing a truncated dengue-2 virus E protein without co-expression of prM protein induces partial protection in mice. Vaccine 19:648\u2013654","journal-title":"Vaccine"},{"key":"90_CR22","doi-asserted-by":"publisher","first-page":"e79","DOI":"10.1016\/S1473-3099(16)30471-6","volume":"17","author":"T Pang","year":"2017","unstructured":"Pang T, Mak TK, Gubler DJ (2017) Prevention and control of dengue-the light at the end of the tunnel. Lancet Infect Dis 17:e79\u2013e87","journal-title":"Lancet Infect Dis"},{"key":"90_CR23","doi-asserted-by":"publisher","first-page":"7135","DOI":"10.1016\/j.vaccine.2015.09.102","volume":"33","author":"KR Porter","year":"2015","unstructured":"Porter KR, Raviprakash K (2015) Nucleic acid (DNA) immunization as a platform for dengue vaccine development. Vaccine 33:7135\u20137140","journal-title":"Vaccine"},{"key":"90_CR24","doi-asserted-by":"publisher","first-page":"90","DOI":"10.1097\/PCC.0b013e3181e911a7","volume":"12","author":"S Ranjit","year":"2011","unstructured":"Ranjit S, Kissoon N (2011) Dengue hemorrhagic fever and shock syndromes. Pediatr Crit Care Med 12:90\u2013100","journal-title":"Pediatr Crit Care Med"},{"key":"90_CR25","doi-asserted-by":"publisher","first-page":"971","DOI":"10.1016\/S0140-6736(97)12483-7","volume":"352","author":"JG Rigau-Perez","year":"1998","unstructured":"Rigau-Perez JG, Clark GG, Gubler DJ, Reiter P, Sanders EJ, Vorndam AV (1998) Dengue and dengue haemorrhagic fever. Lancet 352:971\u2013977","journal-title":"Lancet"},{"key":"90_CR26","doi-asserted-by":"publisher","first-page":"421","DOI":"10.1016\/j.coi.2011.03.008","volume":"23","author":"NY Sardesai","year":"2011","unstructured":"Sardesai NY, Weiner DB (2011) Electroporation delivery of DNA vaccines: prospects for success. Curr Opin Immunol 23:421\u2013429","journal-title":"Curr Opin Immunol"},{"key":"90_CR27","doi-asserted-by":"publisher","first-page":"5751","DOI":"10.1016\/j.vaccine.2016.10.001","volume":"34","author":"Z Sheng","year":"2016","unstructured":"Sheng Z, Gao N, Cui X, Fan D, Chen H, Wu N, Wei J, An J (2016) Electroporation enhances protective immune response of a DNA vaccine against Japanese encephalitis in mice and pigs. Vaccine 34:5751\u20135757","journal-title":"Vaccine"},{"key":"90_CR28","first-page":"515","volume":"61","author":"A Stachyra","year":"2014","unstructured":"Stachyra A, Gora-Sochacka A, Sirko A (2014) DNA vaccines against influenza. Acta Biochim Pol 61:515\u2013522","journal-title":"Acta Biochim Pol"},{"key":"90_CR29","doi-asserted-by":"publisher","first-page":"630","DOI":"10.4049\/jimmunol.1402530","volume":"194","author":"JJ Suschak","year":"2015","unstructured":"Suschak JJ, Wang S, Fitzgerald KA, Lu S (2015) Identification of Aim2 as a sensor for DNA vaccines. J Immunol 194:630\u2013636","journal-title":"J Immunol"},{"key":"90_CR30","doi-asserted-by":"publisher","first-page":"353","DOI":"10.1007\/s12250-014-3542-7","volume":"29","author":"J Wei","year":"2014","unstructured":"Wei J, Chen H, An J (2014) Recent progress in dengue vaccine development. Virol Sin 29:353\u2013363","journal-title":"Virol Sin"},{"key":"90_CR31","doi-asserted-by":"publisher","first-page":"1796","DOI":"10.1038\/mt.2013.119","volume":"21","author":"O Weiland","year":"2013","unstructured":"Weiland O, Ahlen G, Diepolder H, Jung MC, Levander S, Fons M, Mathiesen I, Sardesai NY, Vahlne A, Frelin L, Sallberg M (2013) Therapeutic DNA vaccination using in vivo electroporation followed by standard of care therapy in patients with genotype 1 chronic hepatitis C. Mol Ther 21:1796\u20131805","journal-title":"Mol Ther"},{"key":"90_CR32","unstructured":"WHO (2016) Questions and answers on dengue vaccines. \n                    https:\/\/www.who.int\/immunization\/research\/development\/dengue_q_and_a\/en\/\n                    \n                  . Last updated 20 April 2018"},{"key":"90_CR33","doi-asserted-by":"publisher","first-page":"1200","DOI":"10.1016\/j.vaccine.2016.10.070","volume":"35","author":"WHO","year":"2017","unstructured":"WHO (2017) Dengue vaccine: WHO position paper, July 2016\u2014recommendations. Vaccine 35:1200\u20131201","journal-title":"Vaccine"},{"key":"90_CR34","doi-asserted-by":"publisher","first-page":"3153","DOI":"10.4161\/21645515.2014.980686","volume":"10","author":"B Yang","year":"2014","unstructured":"Yang B, Jeang J, Yang A, Wu TC, Hung CF (2014) DNA vaccine for cancer immunotherapy. Hum Vaccines Immunother 10:3153\u20133164","journal-title":"Hum Vaccines Immunother"},{"key":"90_CR35","doi-asserted-by":"publisher","first-page":"315","DOI":"10.1016\/B978-0-12-800098-4.00007-6","volume":"88","author":"LE Yauch","year":"2014","unstructured":"Yauch LE, Shresta S (2014) Dengue virus vaccine development. Adv Virus Res 88:315\u2013372","journal-title":"Adv Virus Res"},{"key":"90_CR36","doi-asserted-by":"publisher","first-page":"e1003723","DOI":"10.1371\/journal.ppat.1003723","volume":"9","author":"RM Zellweger","year":"2013","unstructured":"Zellweger RM, Miller R, Eddy WE, White LJ, Johnston RE, Shresta S (2013) Role of humoral versus cellular responses induced by a protective dengue vaccine candidate. PLoS Pathog 9:e1003723","journal-title":"PLoS Pathog"},{"key":"90_CR37","doi-asserted-by":"publisher","first-page":"175","DOI":"10.3389\/fcimb.2017.00175","volume":"7","author":"X Zheng","year":"2017","unstructured":"Zheng X, Chen H, Wang R, Fan D, Feng K, Gao N, An J (2017) Effective protection induced by a monovalent DNA vaccine against dengue virus (DV) serotype 1 and a bivalent DNA vaccine against DV1 and DV2 in mice. Front Cell Infect Microbiol 7:175","journal-title":"Front Cell Infect Microbiol"}],"container-title":["Virologica Sinica"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s12250-019-00090-8\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s12250-019-00090-8.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s12250-019-00090-8.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,1,4]],"date-time":"2022-01-04T06:40:17Z","timestamp":1641278417000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s12250-019-00090-8"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,2]]},"references-count":37,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2019,2]]}},"alternative-id":["90"],"URL":"https:\/\/doi.org\/10.1007\/s12250-019-00090-8","relation":{},"ISSN":["1674-0769","1995-820X"],"issn-type":[{"value":"1674-0769","type":"print"},{"value":"1995-820X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,2]]},"assertion":[{"value":"17 October 2018","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"17 January 2019","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"21 February 2019","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Compliance with Ethical Standards"}},{"value":"The authors declare that they have no conflict of interest.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"The whole study was approved by the Institutional Animal Care and Use Committee of Capital Medical University, China (Approval Number: AEEI-2015-066). All institutional and national guidelines for the care and use of laboratory animals were followed.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Animal and Human Rights Statement"}},{"value":"This content has been made available to all.","name":"free","label":"Free to read"}]}}