{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T06:28:00Z","timestamp":1775024880548,"version":"3.50.1"},"reference-count":25,"publisher":"Elsevier BV","issue":"4","license":[{"start":{"date-parts":[[2021,1,5]],"date-time":"2021-01-05T00:00:00Z","timestamp":1609804800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2021,1,5]],"date-time":"2021-01-05T00:00:00Z","timestamp":1609804800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Virol. Sin."],"published-print":{"date-parts":[[2021,8]]},"DOI":"10.1007\/s12250-020-00335-x","type":"journal-article","created":{"date-parts":[[2021,1,5]],"date-time":"2021-01-05T11:14:51Z","timestamp":1609845291000},"page":"636-643","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Phylogenetic Analysis of the Dengue Virus Strains Causing the 2019 Dengue Fever Outbreak in Hainan, China"],"prefix":"10.1016","volume":"36","author":[{"given":"Jiang","family":"Du","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liyuan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoyuan","family":"Hu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruoyan","family":"Peng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gaoyu","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi","family":"Huang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenqi","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kunliang","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiang","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haoxiang","family":"Su","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fan","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yun","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chuanning","family":"Tang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiuji","family":"Cui","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lina","family":"Niu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gang","family":"Lu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0910-5787","authenticated-orcid":false,"given":"Meifang","family":"Xiao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0185-4476","authenticated-orcid":false,"given":"Yongguo","family":"Du","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6195-0614","authenticated-orcid":false,"given":"Feifei","family":"Yin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","published-online":{"date-parts":[[2021,1,5]]},"reference":[{"key":"335_CR1","doi-asserted-by":"publisher","first-page":"1977","DOI":"10.1111\/trf.14130","volume":"57","author":"G Anez","year":"2017","unstructured":"Anez G, Volkova E, Jiang Z, Heisey DAR, Chancey C, Fares RCG, Rios M, Collaborative Study G (2017) Collaborative study to establish World Health Organization international reference reagents for dengue virus Types 1 to 4 RNA for use in nucleic acid testing. Transfusion 57:1977\u20131987","journal-title":"Transfusion"},{"key":"335_CR2","doi-asserted-by":"publisher","first-page":"201","DOI":"10.1016\/j.virusres.2018.07.005","volume":"256","author":"Z Bai","year":"2018","unstructured":"Bai Z, Liu LC, Jiang L, Luo L, Feng H, Lin P, Jing Q, Xiao X, Zhou H, Su W, Cao Y, Li Y, Cao Q, Chen W, Di B, Yang Z (2018) Evolutionary and phylodynamic analyses of Dengue virus serotype I in Guangdong Province, China, between 1985 and 2015. Virus Res 256:201\u2013208","journal-title":"Virus Res"},{"key":"335_CR3","doi-asserted-by":"publisher","first-page":"504","DOI":"10.1038\/nature12060","volume":"496","author":"S Bhatt","year":"2013","unstructured":"Bhatt S, Gething PW, Brady OJ, Messina JP, Farlow AW, Moyes CL, Drake JM, Brownstein JS, Hoen AG, Sankoh O, Myers MF, George DB, Jaenisch T, Wint GR, Simmons CP, Scott TW, Farrar JJ, Hay SI (2013) The global distribution and burden of dengue. Nature 496:504\u2013507","journal-title":"Nature"},{"key":"335_CR4","doi-asserted-by":"publisher","first-page":"e1760","DOI":"10.1371\/journal.pntd.0001760","volume":"6","author":"OJ Brady","year":"2012","unstructured":"Brady OJ, Gething PW, Bhatt S, Messina JP, Brownstein JS, Hoen AG, Moyes CL, Farlow AW, Scott TW, Hay SI (2012) Refining the global spatial limits of dengue virus transmission by evidence-based consensus. PLoS Negl Trop Dis 6:e1760","journal-title":"PLoS Negl Trop Dis"},{"key":"335_CR5","doi-asserted-by":"publisher","first-page":"3011","DOI":"10.1128\/JVI.74.7.3011-3019.2000","volume":"74","author":"S Butrapet","year":"2000","unstructured":"Butrapet S, Huang CY, Pierro DJ, Bhamarapravati N, Gubler DJ, Kinney RM (2000) Attenuation markers of a candidate dengue type 2 vaccine virus, strain 16681 (PDK-53), are defined by mutations in the 5\u2032 noncoding region and nonstructural proteins 1 and 3. J Virol 74:3011\u20133019","journal-title":"J Virol"},{"key":"335_CR6","doi-asserted-by":"publisher","first-page":"649","DOI":"10.1146\/annurev.mi.44.100190.003245","volume":"44","author":"TJ Chambers","year":"1990","unstructured":"Chambers TJ, Hahn CS, Galler R, Rice CM (1990) Flavivirus genome organization, expression, and replication. Annu Rev Microbiol 44:649\u2013688","journal-title":"Annu Rev Microbiol"},{"key":"335_CR7","doi-asserted-by":"publisher","first-page":"1183","DOI":"10.1016\/j.meegid.2011.03.025","volume":"11","author":"S Chen","year":"2011","unstructured":"Chen S (2011) The origin of dengue viruses caused the DF outbreak in Guangdong province, China, in 2006. Infect Genet Evol 11:1183\u20131187","journal-title":"Infect Genet Evol"},{"key":"335_CR8","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1016\/S1567-1348(03)00004-2","volume":"3","author":"EC Holmes","year":"2003","unstructured":"Holmes EC, Twiddy SS (2003) The origin, emergence and evolutionary genetics of dengue virus. Infect Genet Evol 3:19\u201328","journal-title":"Infect Genet Evol"},{"key":"335_CR9","doi-asserted-by":"publisher","first-page":"939","DOI":"10.1101\/gad.297051.117","volume":"31","author":"M Jeske","year":"2017","unstructured":"Jeske M, Muller CW, Ephrussi A (2017) The LOTUS domain is a conserved DEAD-box RNA helicase regulator essential for the recruitment of Vasa to the germ plasm and nuage. Genes Dev 31:939\u2013952","journal-title":"Genes Dev"},{"key":"335_CR10","doi-asserted-by":"publisher","first-page":"542","DOI":"10.3389\/fcimb.2017.00542","volume":"7","author":"R Kumar","year":"2017","unstructured":"Kumar R, Singh N, Abdin MZ, Patel AH, Medigeshi GR (2017) Dengue virus capsid interacts with DDX3X-a potential mechanism for suppression of antiviral functions in dengue infection. Front Cell Infect Microbiol 7:542","journal-title":"Front Cell Infect Microbiol"},{"key":"335_CR11","doi-asserted-by":"publisher","first-page":"1051","DOI":"10.4269\/ajtmh.1986.35.1051","volume":"35","author":"FS Li","year":"1986","unstructured":"Li FS, Yang FR, Song JC, Gao H, Tang JQ, Zou CH, Hu BN, Wen SR, Qiu FX (1986) Etiologic and serologic investigations of the 1980 epidemic of dengue fever on Hainan Island, China. Am J Trop Med Hyg 35:1051\u20131054","journal-title":"Am J Trop Med Hyg"},{"key":"335_CR12","doi-asserted-by":"crossref","unstructured":"Lustig Y, Wolf D, Halutz O, Schwartz E (2017) An outbreak of dengue virus (DENV) type 2 Cosmopolitan genotype in Israeli travellers returning from the Seychelles, April 2017. Euro Surveill 22","DOI":"10.2807\/1560-7917.ES.2017.22.26.30563"},{"key":"335_CR13","doi-asserted-by":"publisher","first-page":"6746","DOI":"10.1128\/JVI.00423-15","volume":"89","author":"NG Naik","year":"2015","unstructured":"Naik NG, Wu HN (2015) Mutation of putative N-glycosylation sites on dengue virus NS4B decreases RNA replication. J Virol 89:6746\u20136760","journal-title":"J Virol"},{"key":"335_CR14","doi-asserted-by":"publisher","first-page":"415","DOI":"10.1126\/science.342.6157.415","volume":"342","author":"D Normile","year":"2013","unstructured":"Normile D (2013) Tropical medicine: surprising new dengue virus throws a spanner in disease control efforts. Science 342:415","journal-title":"Science"},{"key":"335_CR15","doi-asserted-by":"publisher","first-page":"364","DOI":"10.4269\/ajtmh.1991.44.364","volume":"44","author":"FX Qiu","year":"1991","unstructured":"Qiu FX, Chen QQ, Ho QY, Chen WZ, Zhao ZG, Zhao BW (1991) The first epidemic of dengue hemorrhagic fever in the People\u2019s Republic of China. Am J Trop Med Hyg 44:364\u2013370","journal-title":"Am J Trop Med Hyg"},{"key":"335_CR16","first-page":"349","volume":"71","author":"FX Qiu","year":"1993","unstructured":"Qiu FX, Gubler DJ, Liu JC, Chen QQ (1993) Dengue in China: a clinical review. Bull World Health Organ 71:349\u2013359","journal-title":"Bull World Health Organ"},{"key":"335_CR17","doi-asserted-by":"publisher","first-page":"1135","DOI":"10.1086\/651268","volume":"50","author":"A Srikiatkhachorn","year":"2010","unstructured":"Srikiatkhachorn A, Gibbons RV, Green S, Libraty DH, Thomas SJ, Endy TP, Vaughn DW, Nisalak A, Ennis FA, Rothman AL, Nimmannitaya S, Kalayanarooj S (2010) Dengue hemorrhagic fever: the sensitivity and specificity of the world health organization definition for identification of severe cases of dengue in Thailand, 1994-2005. Clin Infect Dis 50:1135\u20131143","journal-title":"Clin Infect Dis"},{"key":"335_CR18","unstructured":"WHO (2009) In: Dengue: Guidelines for Diagnosis, Treatment, Prevention and Control: New Edition. WHO Guidelines Approved by the Guidelines Review Committee. Geneva"},{"key":"335_CR19","doi-asserted-by":"publisher","first-page":"664","DOI":"10.4269\/ajtmh.2010.09-0755","volume":"83","author":"JY Wu","year":"2010","unstructured":"Wu JY, Lun ZR, James AA, Chen XG (2010) Dengue Fever in mainland China. Am J Trop Med Hyg 83:664\u2013671","journal-title":"Am J Trop Med Hyg"},{"key":"335_CR20","doi-asserted-by":"publisher","first-page":"44","DOI":"10.1186\/s40249-016-0131-z","volume":"5","author":"JP Xiao","year":"2016","unstructured":"Xiao JP, He JF, Deng AP, Lin HL, Song T, Peng ZQ, Wu XC, Liu T, Li ZH, Rutherford S, Zeng WL, Li X, Ma WJ, Zhang YH (2016) Characterizing a large outbreak of dengue fever in Guangdong Province, China. Infect Dis Poverty 5:44","journal-title":"Infect Dis Poverty"},{"key":"335_CR21","doi-asserted-by":"publisher","first-page":"1182","DOI":"10.4269\/ajtmh.2007.76.1182","volume":"76","author":"G Xu","year":"2007","unstructured":"Xu G, Dong H, Shi N, Liu S, Zhou A, Cheng Z, Chen G, Liu J, Fang T, Zhang H, Gu C, Tan X, Ye J, Xie S, Cao G (2007) An outbreak of dengue virus serotype 1 infection in Cixi, Ningbo, People\u2019s Republic of China, 2004, associated with a traveler from Thailand and high density of Aedes albopictus. Am J Trop Med Hyg 76:1182\u20131188","journal-title":"Am J Trop Med Hyg"},{"key":"335_CR22","doi-asserted-by":"publisher","first-page":"870","DOI":"10.4269\/ajtmh.18-0951","volume":"101","author":"J Yu","year":"2019","unstructured":"Yu J, Li X, He X, Liu X, Zhong Z, Xie Q, Zhu L, Jia F, Mao Y, Chen Z, Wen Y, Ma D, Yu L, Zhang B, Zhao W, Xiao W (2019) Epidemiological and evolutionary analysis of dengue-1 virus detected in guangdong during 2014: recycling of old and formation of new lineages. Am J Trop Med Hyg 101:870\u2013883","journal-title":"Am J Trop Med Hyg"},{"key":"335_CR23","doi-asserted-by":"publisher","first-page":"82","DOI":"10.1016\/j.ijid.2019.06.015","volume":"86","author":"Y Yue","year":"2019","unstructured":"Yue Y, Liu X, Xu M, Ren D, Liu Q (2019) Epidemiological dynamics of dengue fever in mainland China, 2014\u20132018. Int J Infect Dis 86:82\u201393","journal-title":"Int J Infect Dis"},{"key":"335_CR24","doi-asserted-by":"publisher","first-page":"73","DOI":"10.1017\/S0950268808000617","volume":"137","author":"K Zheng","year":"2009","unstructured":"Zheng K, Zhou HQ, Yan J, Ke CW, Maeda A, Maeda J, Takashima I, Kurane I, Ma H, Xie XM (2009) Molecular characterization of the E gene of dengue virus type 1 isolated in Guangdong province, China, in 2006. Epidemiol Infect 137:73\u201378","journal-title":"Epidemiol Infect"},{"key":"335_CR25","doi-asserted-by":"publisher","first-page":"e22","DOI":"10.1038\/emi.2016.48","volume":"5","author":"Z Zhu","year":"2016","unstructured":"Zhu Z, Chan JF, Tee KM, Choi GK, Lau SK, Woo PC, Tse H, Yuen KY (2016) Comparative genomic analysis of pre-epidemic and epidemic Zika virus strains for virological factors potentially associated with the rapidly expanding epidemic. Emerg Microbes Infect 5:e22","journal-title":"Emerg Microbes Infect"}],"container-title":["Virologica Sinica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s12250-020-00335-x.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s12250-020-00335-x\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s12250-020-00335-x.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,1,4]],"date-time":"2022-01-04T06:37:38Z","timestamp":1641278258000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s12250-020-00335-x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,1,5]]},"references-count":25,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2021,8]]}},"alternative-id":["335"],"URL":"https:\/\/doi.org\/10.1007\/s12250-020-00335-x","relation":{},"ISSN":["1674-0769","1995-820X"],"issn-type":[{"value":"1674-0769","type":"print"},{"value":"1995-820X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,1,5]]},"assertion":[{"value":"16 August 2020","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"3 December 2020","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"5 January 2021","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Compliance with Ethical Standards"}},{"value":"The authors declare that they have no conflict of interest.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"This study is approved by the Ethics Committee of the Hainan Medical University. The research content and significance of our study were explained and written informed consents were obtained by all the dengue patients and patient\u2019s guardians. All experiments were performed in accordance with the experimental guidelines for molecular biology, and abide by biosafety regulations.\u00a0All methods involving humans were performed in accordance with the guidelines and regulations of the Declaration of Helsinki.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Animal and Human Rights Statement"}},{"value":"This content has been made available to all.","name":"free","label":"Free to read"}]}}