{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T12:29:08Z","timestamp":1787315348265,"version":"build-2736575974"},"reference-count":58,"publisher":"Springer Science and Business Media LLC","issue":"2","license":[{"start":{"date-parts":[[2022,7,12]],"date-time":"2022-07-12T00:00:00Z","timestamp":1657584000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2022,7,12]],"date-time":"2022-07-12T00:00:00Z","timestamp":1657584000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Neurosci. Bull."],"published-print":{"date-parts":[[2023,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Post-amputation pain causes great suffering to amputees, but still no effective drugs are available due to its elusive mechanisms. Our previous clinical studies found that surgical removal or radiofrequency treatment of the neuroma at the axotomized nerve stump effectively relieves the phantom pain afflicting patients after amputation. This indicated an essential role of the residual nerve stump in the formation of chronic post-amputation pain (CPAP). However, the molecular mechanism by which the residual nerve stump or neuroma is involved and regulates CPAP is still a mystery. In this study, we found that nociceptors expressed the mechanosensitive ion channel TMEM63A and macrophages infiltrated into the dorsal root ganglion (DRG) neurons worked synergistically to promote CPAP. Histology and qRT-PCR showed that TMEM63A was mainly expressed in mechanical pain-producing non-peptidergic nociceptors in the DRG, and the expression of TMEM63A increased significantly both in the neuroma from amputated patients and the DRG in a mouse model of tibial nerve transfer (TNT). Behavioral tests showed that the mechanical, heat, and cold sensitivity were not affected in the\n                    <jats:italic>Tmem63a<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>-\/-<\/jats:italic>\n                    <\/jats:sup>\n                    mice in the na\u00efve state, suggesting the basal pain was not affected. In the inflammatory and post-amputation state, the mechanical allodynia but not the heat hyperalgesia or cold allodynia was significantly decreased in\n                    <jats:italic>Tmem63a<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>-\/-<\/jats:italic>\n                    <\/jats:sup>\n                    mice. Further study showed that there was severe neuronal injury and macrophage infiltration in the DRG, tibial nerve, residual stump, and the neuroma-like structure of the TNT mouse model, Consistent with this, expression of the pro-inflammatory cytokines TNF-\u03b1, IL-6, and IL-1\u03b2 all increased dramatically in the DRG. Interestingly, the deletion of\n                    <jats:italic>Tmem63a<\/jats:italic>\n                    significantly reduced the macrophage infiltration in the DRG but not in the tibial nerve stump. Furthermore, the ablation of macrophages significantly reduced both the expression of\n                    <jats:italic>Tmem63a<\/jats:italic>\n                    and the mechanical allodynia in the TNT mouse model, indicating an interaction between nociceptors and macrophages, and that these two factors gang up together to regulate the formation of CPAP. This provides a new insight into the mechanisms underlying CPAP and potential drug targets its treatment.\n                  <\/jats:p>","DOI":"10.1007\/s12264-022-00910-0","type":"journal-article","created":{"date-parts":[[2022,7,12]],"date-time":"2022-07-12T14:02:54Z","timestamp":1657634574000},"page":"177-193","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Mechanosensitive Ion Channel TMEM63A Gangs Up with Local Macrophages to Modulate Chronic Post-amputation Pain"],"prefix":"10.1007","volume":"39","author":[{"given":"Shaofeng","family":"Pu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiyang","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fang","family":"Tong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wan-Jie","family":"Du","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuai","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huan","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziyue","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaomeng","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingjian","family":"Han","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongping","family":"Du","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,7,12]]},"reference":[{"key":"910_CR1","doi-asserted-by":"publisher","first-page":"551","DOI":"10.1097\/AJP.0b013e318261c9f9","volume":"29","author":"C Clarke","year":"2013","unstructured":"Clarke C, Lindsay DR, Pyati S, Buchheit T. Residual limb pain is not a diagnosis: A proposed algorithm to classify postamputation pain. Clin J Pain 2013, 29: 551\u2013562.","journal-title":"Clin J Pain"},{"issue":"103","key":"910_CR2","first-page":"19","volume":"2020","author":"JM Modest","year":"2013","unstructured":"Modest JM, Raducha JE, Testa EJ, Eberson CP. Management of post-amputation pain. R I Med J 2013, 2020(103): 19\u201322.","journal-title":"R I Med J"},{"key":"910_CR3","doi-asserted-by":"publisher","first-page":"182","DOI":"10.1016\/S1474-4422(02)00074-1","volume":"1","author":"H Flor","year":"2002","unstructured":"Flor H. Phantom-limb pain: Characteristics, causes, and treatment. Lancet Neurol 2002, 1: 182\u2013189.","journal-title":"Lancet Neurol"},{"key":"910_CR4","doi-asserted-by":"publisher","first-page":"748","DOI":"10.3389\/fneur.2018.00748","volume":"9","author":"M Ortiz-Catalan","year":"2018","unstructured":"Ortiz-Catalan M. The stochastic entanglement and phantom motor execution hypotheses: A theoretical framework for the origin and treatment of phantom limb pain. Front Neurol 2018, 9: 748.","journal-title":"Front Neurol"},{"key":"910_CR5","first-page":"211","volume":"69","author":"T Bloomquist","year":"2001","unstructured":"Bloomquist T. Amputation and phantom limb pain: A pain-prevention model. AANA J 2001, 69: 211\u2013217.","journal-title":"AANA J"},{"key":"910_CR6","doi-asserted-by":"publisher","first-page":"1944","DOI":"10.1111\/pme.12254","volume":"14","author":"K Ma","year":"2013","unstructured":"Ma K, Zhou QH, Xu YM, Xu T, Du DP, Huang XH. Peripheral nerve adjustment for postherpetic neuralgia: A randomized, controlled clinical study. Pain Med 2013, 14: 1944\u20131953.","journal-title":"Pain Med"},{"key":"910_CR7","doi-asserted-by":"publisher","first-page":"3437","DOI":"10.2147\/JPR.S283986","volume":"13","author":"SF Pu","year":"2020","unstructured":"Pu SF, Wu JZ, Han QJ, Zhang X, Lv YY, Xu YM, et al. Ultrasonography-guided radiofrequency ablation for painful stump neuromas to relieve postamputation pain: A pilot study. J Pain Res 2020, 13: 3437\u20133445.","journal-title":"J Pain Res"},{"key":"910_CR8","doi-asserted-by":"publisher","first-page":"1940","DOI":"10.1093\/pm\/pnab008","volume":"22","author":"JZ Wu","year":"2021","unstructured":"Wu JZ, Xu YM, Pu SF, Zhou J, Lv YY, Li C, et al. US-guided transforaminal cervical nerve root block: A novel lateral in-plane approach. Pain Med 2021, 22: 1940\u20131945.","journal-title":"Pain Med"},{"key":"910_CR9","doi-asserted-by":"publisher","first-page":"171","DOI":"10.2147\/JPR.S181915","volume":"12","author":"X Zhang","year":"2018","unstructured":"Zhang X, Shi HF, Zhou J, Xu YM, Pu SF, Lv YY, et al. The effectiveness of ultrasound-guided cervical transforaminal epidural steroid injections in cervical radiculopathy: A prospective pilot study. J Pain Res 2018, 12: 171\u2013177.","journal-title":"J Pain Res"},{"key":"910_CR10","doi-asserted-by":"publisher","first-page":"295","DOI":"10.2147\/JPR.S127157","volume":"10","author":"X Zhang","year":"2017","unstructured":"Zhang X, Xu YM, Zhou J, Pu SF, Lv YY, Chen YP, et al. Ultrasound-guided alcohol neurolysis and radiofrequency ablation of painful stump neuroma: Effective treatments for post-amputation pain. J Pain Res 2017, 10: 295\u2013302.","journal-title":"J Pain Res"},{"key":"910_CR11","doi-asserted-by":"publisher","first-page":"275","DOI":"10.3233\/BMR-130444","volume":"27","author":"S Kesikburun","year":"2014","unstructured":"Kesikburun S, Ya\u015far E, Dede \u0130, G\u00f6ktepe S, Tan AK. Ultrasound-guided steroid injection in the treatment of stump neuroma: Pilot study. J Back Musculoskelet Rehabilitation 2014, 27: 275\u2013279.","journal-title":"J Back Musculoskelet Rehabilitation"},{"key":"910_CR12","doi-asserted-by":"publisher","first-page":"214","DOI":"10.1097\/j.pain.0000000000001101","volume":"159","author":"LH Poppler","year":"2018","unstructured":"Poppler LH, Parikh RP, Bichanich MJ, Rebehn K, Bettlach CR, MacKinnon SE, et al. Surgical interventions for the treatment of painful neuroma: A comparative meta-analysis. Pain 2018, 159: 214\u2013223.","journal-title":"Pain"},{"key":"910_CR13","doi-asserted-by":"publisher","DOI":"10.1097\/MD.0000000000015142","volume":"98","author":"WY Chen","year":"2019","unstructured":"Chen WY, Zhang H, Huang JY, Li YX, Zhang Z, Peng YL. Traumatic neuroma in mastectomy scar: Two case reports and review of the literature. Medicine 2019, 98: e15142.","journal-title":"Medicine"},{"key":"910_CR14","doi-asserted-by":"publisher","first-page":"242","DOI":"10.1186\/s12957-016-0990-6","volume":"14","author":"J Kang","year":"2016","unstructured":"Kang J, Yang PL, Zang QJ, He XJ. Traumatic neuroma of the superficial peroneal nerve in a patient: A case report and review of the literature. World J Surg Oncol 2016, 14: 242.","journal-title":"World J Surg Oncol"},{"key":"910_CR15","doi-asserted-by":"publisher","first-page":"1162","DOI":"10.1016\/j.neuron.2015.08.032","volume":"87","author":"SS Ranade","year":"2015","unstructured":"Ranade SS, Syeda R, Patapoutian A. Mechanically activated ion channels. Neuron 2015, 87: 1162\u20131179.","journal-title":"Neuron"},{"key":"910_CR16","doi-asserted-by":"publisher","first-page":"567","DOI":"10.1038\/s41586-020-2933-1","volume":"587","author":"JM Kefauver","year":"2020","unstructured":"Kefauver JM, Ward AB, Patapoutian A. Discoveries in structure and physiology of mechanically activated ion channels. Nature 2020, 587: 567\u2013576.","journal-title":"Nature"},{"key":"910_CR17","doi-asserted-by":"publisher","first-page":"1419","DOI":"10.1016\/j.celrep.2019.01.056","volume":"26","author":"MM Zhang","year":"2019","unstructured":"Zhang MM, Wang YF, Geng J, Zhou SQ, Xiao BL. Mechanically activated piezo channels mediate touch and suppress acute mechanical pain response in mice. Cell Rep 2019, 26: 1419-1431.e4.","journal-title":"Cell Rep"},{"key":"910_CR18","doi-asserted-by":"publisher","first-page":"207","DOI":"10.1146\/annurev-neuro-070918-050509","volume":"43","author":"P Jin","year":"2020","unstructured":"Jin P, Jan LY, Jan YN. Mechanosensitive ion channels: Structural features relevant to mechanotransduction mechanisms. Annu Rev Neurosci 2020, 43: 207\u2013229.","journal-title":"Annu Rev Neurosci"},{"key":"910_CR19","doi-asserted-by":"publisher","first-page":"eaat9892","DOI":"10.1126\/scitranslmed.aat9892","volume":"10","author":"M Szczot","year":"2018","unstructured":"Szczot M, Liljencrantz J, Ghitani N, Barik A, Lam R, Thompson JH, et al. PIEZO2 mediates injury-induced tactile pain in mice and humans. Sci Transl Med 2018, 10: eaat9892.","journal-title":"Sci Transl Med"},{"key":"910_CR20","doi-asserted-by":"publisher","first-page":"956","DOI":"10.1016\/j.cell.2020.01.033","volume":"180","author":"L Beaulieu-Laroche","year":"2020","unstructured":"Beaulieu-Laroche L, Christin M, Donoghue A, Agosti F, Yousefpour N, Petitjean H, et al. TACAN is an ion channel involved in sensing mechanical pain. Cell 2020, 180: 956-967.e17.","journal-title":"Cell"},{"key":"910_CR21","doi-asserted-by":"publisher","first-page":"41844","DOI":"10.7554\/eLife.41844","volume":"7","author":"SE Murthy","year":"2018","unstructured":"Murthy SE, Dubin AE, Whitwam T, Jojoa-Cruz S, Cahalan SM, Mousavi SAR, et al. OSCA\/TMEM63 are an evolutionarily conserved family of mechanically activated ion channels. eLife 2018, 7: 41844.","journal-title":"eLife"},{"key":"910_CR22","doi-asserted-by":"publisher","first-page":"996","DOI":"10.1016\/j.ajhg.2019.09.011","volume":"105","author":"HF Yan","year":"2019","unstructured":"Yan HF, Helman G, Murthy SE, Ji HR, Crawford J, Kubisiak T, et al. Heterozygous variants in the mechanosensitive ion channel TMEM63A result in transient hypomyelination during infancy. Am J Hum Genet 2019, 105: 996\u20131004.","journal-title":"Am J Hum Genet"},{"key":"910_CR23","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2020.107596","volume":"31","author":"H Du","year":"2020","unstructured":"Du H, Ye C, Wu D, Zang YY, Zhang LQ, Chen C, et al. The cation channel TMEM63B is an osmosensor required for hearing. Cell Rep 2020, 31: 107596.","journal-title":"Cell Rep"},{"key":"910_CR24","doi-asserted-by":"publisher","first-page":"e42068","DOI":"10.7554\/eLife.42068","volume":"8","author":"A Schulz","year":"2019","unstructured":"Schulz A, M\u00fcller NV, van de Lest NA, Eisenreich A, Schmidbauer M, Barysenka A, et al. Analysis of the genomic architecture of a complex trait locus in hypertensive rat models links Tmem63c to kidney damage. eLife 2019, 8: e42068.","journal-title":"eLife"},{"key":"910_CR25","doi-asserted-by":"publisher","first-page":"6848","DOI":"10.1523\/JNEUROSCI.3257-18.2019","volume":"39","author":"X Luo","year":"2019","unstructured":"Luo X, Huh Y, Bang SS, He QR, Zhang LL, Matsuda M, et al. Macrophage toll-like receptor 9 contributes to chemotherapy-induced neuropathic pain in male mice. J Neurosci 2019, 39: 6848\u20136864.","journal-title":"J Neurosci"},{"key":"910_CR26","doi-asserted-by":"publisher","first-page":"1527","DOI":"10.1016\/j.pain.2014.04.029","volume":"155","author":"MK McDonald","year":"2014","unstructured":"McDonald MK, Tian YZ, Qureshi RA, Gormley M, Ertel A, Gao R, et al. Functional significance of macrophage-derived exosomes in inflammation and pain. Pain 2014, 155: 1527\u20131539.","journal-title":"Pain"},{"key":"910_CR27","doi-asserted-by":"publisher","first-page":"53","DOI":"10.1186\/s12974-017-0828-3","volume":"14","author":"JJ Wu","year":"2017","unstructured":"Wu JJ, Xie HY, Yao SZ, Liang YC. Macrophage and nerve interaction in endometriosis. J Neuroinflammation 2017, 14: 53.","journal-title":"J Neuroinflammation"},{"key":"910_CR28","doi-asserted-by":"publisher","first-page":"12189","DOI":"10.7150\/thno.48028","volume":"10","author":"CY Yin","year":"2020","unstructured":"Yin CY, Liu BY, Li YY, Li XJ, Wang J, Chen RX, et al. IL-33\/ST2 induces neutrophil-dependent reactive oxygen species production and mediates gout pain. Theranostics 2020, 10: 12189\u201312203.","journal-title":"Theranostics"},{"key":"910_CR29","doi-asserted-by":"publisher","first-page":"264","DOI":"10.1038\/s41467-019-13839-2","volume":"11","author":"XB Yu","year":"2020","unstructured":"Yu XB, Liu HJ, Hamel KA, Morvan MG, Yu S, Leff J, et al. Dorsal root ganglion macrophages contribute to both the initiation and persistence of neuropathic pain. Nat Commun 2020, 11: 264.","journal-title":"Nat Commun"},{"key":"910_CR30","doi-asserted-by":"publisher","first-page":"3568","DOI":"10.1172\/JCI99888","volume":"128","author":"SS Bang","year":"2018","unstructured":"Bang SS, Xie YK, Zhang ZJ, Wang ZL, Xu ZZ, Ji RR. GPR37 regulates macrophage phagocytosis and resolution of inflammatory pain. J Clin Invest 2018, 128: 3568\u20133582.","journal-title":"J Clin Invest"},{"key":"910_CR31","doi-asserted-by":"publisher","first-page":"433","DOI":"10.1038\/s41577-019-0147-2","volume":"19","author":"P Baral","year":"2019","unstructured":"Baral P, Udit S, Chiu IM. Pain and immunity: Implications for host defence. Nat Rev Immunol 2019, 19: 433\u2013447.","journal-title":"Nat Rev Immunol"},{"key":"910_CR32","doi-asserted-by":"publisher","first-page":"17","DOI":"10.1016\/j.conb.2019.11.006","volume":"62","author":"OY Chen","year":"2020","unstructured":"Chen OY, Donnelly CR, Ji RR. Regulation of pain by neuro-immune interactions between macrophages and nociceptor sensory neurons. Curr Opin Neurobiol 2020, 62: 17\u201325.","journal-title":"Curr Opin Neurobiol"},{"key":"910_CR33","doi-asserted-by":"publisher","first-page":"572","DOI":"10.1126\/science.aaf8924","volume":"354","author":"RR Ji","year":"2016","unstructured":"Ji RR, Chamessian A, Zhang YQ. Pain regulation by non-neuronal cells and inflammation. Science 2016, 354: 572\u2013577.","journal-title":"Science"},{"key":"910_CR34","doi-asserted-by":"publisher","first-page":"27","DOI":"10.1186\/1742-2094-7-27","volume":"7","author":"L Leung","year":"2010","unstructured":"Leung L, Cahill CM. TNF-alpha and neuropathic pain\u2014a review. J Neuroinflammation 2010, 7: 27.","journal-title":"J Neuroinflammation"},{"key":"910_CR35","doi-asserted-by":"crossref","unstructured":"Lv D, Song H, Shi G. Anti-TNF-alpha treatment for pelvic pain associated with endometriosis. Cochrane Database Syst Rev 2010: CD008088.","DOI":"10.1002\/14651858.CD008088"},{"key":"910_CR36","doi-asserted-by":"publisher","first-page":"15072","DOI":"10.1523\/JNEUROSCI.2443-11.2011","volume":"31","author":"CK Park","year":"2011","unstructured":"Park CK, L\u00fc N, Xu ZZ, Liu T, Serhan CN, Ji RR. Resolving TRPV1- and TNF-\u03b1-mediated spinal cord synaptic plasticity and inflammatory pain with neuroprotectin D1. J Neurosci 2011, 31: 15072\u201315085.","journal-title":"J Neurosci"},{"key":"910_CR37","doi-asserted-by":"publisher","first-page":"419","DOI":"10.1016\/j.pain.2010.11.014","volume":"152","author":"L Zhang","year":"2011","unstructured":"Zhang L, Berta T, Xu ZZ, Liu T, Park JY, Ji RR. TNF-alpha contributes to spinal cord synaptic plasticity and inflammatory pain: Distinct role of TNF receptor subtypes 1 and 2. Pain 2011, 152: 419\u2013427.","journal-title":"Pain"},{"key":"910_CR38","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2019.104402","volume":"149","author":"F Atzeni","year":"2019","unstructured":"Atzeni F, Nucera V, Masala IF, Sarzi-Puttini P, Bonitta G. Il-6 Involvement in pain, fatigue and mood disorders in rheumatoid arthritis and the effects of Il-6 inhibitor sarilumab. Pharmacol Res 2019, 149: 104402.","journal-title":"Pharmacol Res"},{"key":"910_CR39","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2020.107657","volume":"31","author":"ZS Hu","year":"2020","unstructured":"Hu ZS, Deng N, Liu KL, Zhou N, Sun Y, Zeng WW. CNTF-STAT3-IL-6 axis mediates neuroinflammatory cascade across schwann cell-neuron-microglia. Cell Rep 2020, 31: 107657.","journal-title":"Cell Rep"},{"key":"910_CR40","doi-asserted-by":"publisher","first-page":"141","DOI":"10.1186\/s12974-016-0607-6","volume":"13","author":"YQ Zhou","year":"2016","unstructured":"Zhou YQ, Liu Z, Liu ZH, Chen SP, Li M, Shahveranov A, et al. Interleukin-6: An emerging regulator of pathological pain. J Neuroinflammation 2016, 13: 141.","journal-title":"J Neuroinflammation"},{"key":"910_CR41","doi-asserted-by":"publisher","first-page":"733","DOI":"10.1007\/s11064-015-1521-5","volume":"40","author":"QD Shao","year":"2015","unstructured":"Shao QD, Li YF, Wang Q, Zhao JN. IL-10 and IL-1\u03b2 mediate neuropathic-pain like behavior in the ventrolateral orbital cortex. Neurochem Res 2015, 40: 733\u2013739.","journal-title":"Neurochem Res"},{"key":"910_CR42","doi-asserted-by":"publisher","first-page":"34","DOI":"10.1186\/s12974-019-1411-x","volume":"16","author":"HC Xiang","year":"2019","unstructured":"Xiang HC, Lin LX, Hu XF, Zhu H, Li HP, Zhang RY, et al. AMPK activation attenuates inflammatory pain through inhibiting NF-\u03baB activation and IL-1\u03b2 expression. J Neuroinflammation 2019, 16: 34.","journal-title":"J Neuroinflammation"},{"key":"910_CR43","doi-asserted-by":"publisher","first-page":"14062","DOI":"10.1523\/JNEUROSCI.3795-08.2008","volume":"28","author":"AM Binshtok","year":"2008","unstructured":"Binshtok AM, Wang HB, Zimmermann K, Amaya F, Vardeh D, Shi L, et al. Nociceptors are interleukin-1beta sensors. J Neurosci 2008, 28: 14062\u201314073.","journal-title":"J Neurosci"},{"key":"910_CR44","doi-asserted-by":"publisher","first-page":"71","DOI":"10.1016\/j.jneuroim.2018.03.017","volume":"319","author":"K Serizawa","year":"2018","unstructured":"Serizawa K, Tomizawa-Shinohara H, Magi M, Yogo K, Matsumoto Y. Anti-IL-6 receptor antibody improves pain symptoms in mice with experimental autoimmune encephalomyelitis. J Neuroimmunol 2018, 319: 71\u201379.","journal-title":"J Neuroimmunol"},{"key":"910_CR45","doi-asserted-by":"publisher","first-page":"435","DOI":"10.1007\/s12264-021-00805-6","volume":"38","author":"F Tong","year":"2022","unstructured":"Tong F, He QR, Du WJ, Yang H, Du DP, Pu SF, et al. Sympathetic nerve mediated spinal glia activation underlies itch in a cutaneous T-cell lymphoma model. Neurosci Bull 2022, 38: 435\u2013439.","journal-title":"Neurosci Bull"},{"key":"910_CR46","doi-asserted-by":"publisher","first-page":"1279","DOI":"10.1016\/j.neuron.2016.11.007","volume":"92","author":"QJ Han","year":"2016","unstructured":"Han QJ, Kim YH, Wang XM, Liu D, Zhang ZJ, Bey AL, et al. SHANK3 deficiency impairs heat hyperalgesia and TRPV1 signaling in primary sensory neurons. Neuron 2016, 92: 1279\u20131293.","journal-title":"Neuron"},{"key":"910_CR47","doi-asserted-by":"publisher","first-page":"47","DOI":"10.1016\/S0149-7634(05)80090-9","volume":"15","author":"WJ Dixon","year":"1991","unstructured":"Dixon WJ. Staircase bioassay: The up-and-down method. Neurosci Biobehav Rev 1991, 15: 47\u201350.","journal-title":"Neurosci Biobehav Rev"},{"key":"910_CR48","doi-asserted-by":"publisher","first-page":"320","DOI":"10.1016\/j.pain.2007.06.030","volume":"134","author":"MJ Dorsi","year":"2008","unstructured":"Dorsi MJ, Chen L, Murinson BB, Pogatzki-Zahn EM, Meyer RA, Belzberg AJ. The tibial neuroma transposition (TNT) model of neuroma pain and hyperalgesia. Pain 2008, 134: 320\u2013334.","journal-title":"Pain"},{"key":"910_CR49","doi-asserted-by":"publisher","first-page":"145","DOI":"10.1038\/nn.3881","volume":"18","author":"D Usoskin","year":"2015","unstructured":"Usoskin D, Furlan A, Islam S, Abdo H, L\u00f6nnerberg P, Lou DH, et al. Unbiased classification of sensory neuron types by large-scale single-cell RNA sequencing. Nat Neurosci 2015, 18: 145\u2013153.","journal-title":"Nat Neurosci"},{"key":"910_CR50","doi-asserted-by":"publisher","first-page":"967","DOI":"10.1038\/cr.2016.90","volume":"26","author":"CL Li","year":"2016","unstructured":"Li CL, Li KC, Wu D, Chen Y, Luo H, Zhao JR, et al. Somatosensory neuron types identified by high-coverage single-cell RNA-sequencing and functional heterogeneity. Cell Res 2016, 26: 967.","journal-title":"Cell Res"},{"key":"910_CR51","doi-asserted-by":"publisher","first-page":"367","DOI":"10.1111\/j.1460-9568.1995.tb00332.x","volume":"7","author":"X Zhang","year":"1995","unstructured":"Zhang X, Ji RR, Nilsson S, Villar M, Ubink R, Ju G, et al. Neuropeptide Y and galanin binding sites in rat and monkey lumbar dorsal root ganglia and spinal cord and effect of peripheral axotomy. Eur J Neurosci 1995, 7: 367\u2013380.","journal-title":"Eur J Neurosci"},{"key":"910_CR52","first-page":"4105","volume":"1960","author":"SB Weisser","year":"2012","unstructured":"Weisser SB, van Rooijen N, Sly LM. Depletion and reconstitution of macrophages in mice. J Vis Exp 2012, 1960: 4105.","journal-title":"J Vis Exp"},{"key":"910_CR53","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2019.113121","volume":"324","author":"NP Staff","year":"2020","unstructured":"Staff NP, Fehrenbacher JC, Caillaud M, Damaj MI, Segal RA, Rieger S. Pathogenesis of paclitaxel-induced peripheral neuropathy: A current review of in vitro and in vivo findings using rodent and human model systems. Exp Neurol 2020, 324: 113121.","journal-title":"Exp Neurol"},{"key":"910_CR54","doi-asserted-by":"publisher","first-page":"958","DOI":"10.1038\/nri2448","volume":"8","author":"DM Mosser","year":"2008","unstructured":"Mosser DM, Edwards JP. Exploring the full spectrum of macrophage activation. Nat Rev Immunol 2008, 8: 958\u2013969.","journal-title":"Nat Rev Immunol"},{"key":"910_CR55","doi-asserted-by":"publisher","first-page":"1704","DOI":"10.1038\/s41467-021-21940-8","volume":"12","author":"SS Bang","year":"2021","unstructured":"Bang SS, Donnelly CR, Luo X, Toro-Moreno M, Tao XS, Wang ZL, et al. Activation of GPR37 in macrophages confers protection against infection-induced sepsis and pain-like behaviour in mice. Nat Commun 2021, 12: 1704.","journal-title":"Nat Commun"},{"key":"910_CR56","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.787565","volume":"12","author":"J Ji","year":"2021","unstructured":"Ji J, He QR, Luo X, Bang SS, Matsuoka Y, McGinnis A, et al. IL-23 enhances C-fiber-mediated and blue light-induced spontaneous pain in female mice. Front Immunol 2021, 12: 787565.","journal-title":"Front Immunol"},{"key":"910_CR57","doi-asserted-by":"publisher","first-page":"2691","DOI":"10.1016\/j.neuron.2021.06.015","volume":"109","author":"X Luo","year":"2021","unstructured":"Luo X, Chen OY, Wang ZL, Bang SS, Ji J, Lee SH, et al. IL-23\/IL-17A\/TRPV1 axis produces mechanical pain via macrophage-sensory neuron crosstalk in female mice. Neuron 2021, 109: 2691-2706.e5.","journal-title":"Neuron"},{"key":"910_CR58","doi-asserted-by":"publisher","first-page":"213","DOI":"10.1016\/j.bbi.2014.10.009","volume":"44","author":"BC Sutton","year":"2015","unstructured":"Sutton BC, Opp MR. Acute increases in intramuscular inflammatory cytokines are necessary for the development of mechanical hypersensitivity in a mouse model of musculoskeletal sensitization. Brain Behav Immun 2015, 44: 213\u2013220.","journal-title":"Brain Behav Immun"}],"container-title":["Neuroscience Bulletin"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s12264-022-00910-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s12264-022-00910-0\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s12264-022-00910-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,5]],"date-time":"2024-08-05T12:44:09Z","timestamp":1722861849000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s12264-022-00910-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,7,12]]},"references-count":58,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2023,2]]}},"alternative-id":["910"],"URL":"https:\/\/doi.org\/10.1007\/s12264-022-00910-0","relation":{},"ISSN":["1673-7067","1995-8218"],"issn-type":[{"value":"1673-7067","type":"print"},{"value":"1995-8218","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,7,12]]},"assertion":[{"value":"31 December 2021","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"16 May 2022","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"12 July 2022","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors have no conflict of interest in this paper.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}}]}}