{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T21:53:36Z","timestamp":1777413216941,"version":"3.51.4"},"reference-count":14,"publisher":"Springer Science and Business Media LLC","issue":"4","license":[{"start":{"date-parts":[[2024,2,26]],"date-time":"2024-02-26T00:00:00Z","timestamp":1708905600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2024,2,26]],"date-time":"2024-02-26T00:00:00Z","timestamp":1708905600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81570464"],"award-info":[{"award-number":["81570464"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81770271"],"award-info":[{"award-number":["81770271"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Municipal Planning Projects of Scientific Technology of Guangzhou","award":["201804020083"],"award-info":[{"award-number":["201804020083"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["J. of Cardiovasc. Trans. Res."],"published-print":{"date-parts":[[2024,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The mouse aortic transplantation model is a valuable tool for investigating the mechanisms of atherosclerosis regression, but few laboratories can generate it due to the operation difficulty, especially for the style of end-to-side anastomosis, which facilitates syngeneic heterotopic transplanting a plaque-rich aortic arch into the abdominal aorta. Here we provide a modified protocol for generating this allograft model, which is capable of overcoming several critical surgical challenges such as separating a longer abdominal aorta segment, reducing bleeding and thrombosis, optimizing aortotomy, and improving end-to-side anastomosis to guarantee a potent graft. By transplanting plaque-rich aortic arches into the abdominal aorta of wildtype mice, a high operation success rate (over 90%) was noted with aortic clamping time under 60\u00a0min, the graft potency was satisfactory evidenced by examinations of micro-CT, ultrasound, and lower limb blood flow measurement, while a significant atherosclerosis regression was observed in the grafts at 1\u00a0week after transplantation.<\/jats:p>\n                <jats:p><jats:bold>Graphical Abstract<\/jats:bold><\/jats:p>","DOI":"10.1007\/s12265-024-10495-w","type":"journal-article","created":{"date-parts":[[2024,2,26]],"date-time":"2024-02-26T19:02:22Z","timestamp":1708974142000},"page":"901-909","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Allograft Model of Aortic Arch Segment Grafting to Abdominal Aorta Through End-to-Side Anastomosis in Mice"],"prefix":"10.1007","volume":"17","author":[{"given":"Chiyu","family":"Liu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qi","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mingyuan","family":"He","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5961-390X","authenticated-orcid":false,"given":"Yulin","family":"Liao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2024,2,26]]},"reference":[{"issue":"2","key":"10495_CR1","doi-asserted-by":"publisher","first-page":"171","DOI":"10.1016\/S0022-4804(03)00039-8","volume":"111","author":"I Chereshnev","year":"2003","unstructured":"Chereshnev I, et al. Mouse model of heterotopic aortic arch transplantation. J Surg Res. 2003;111(2):171\u20136.","journal-title":"J Surg Res"},{"issue":"2","key":"10495_CR2","doi-asserted-by":"publisher","first-page":"707","DOI":"10.1016\/j.jss.2015.08.039","volume":"200","author":"P Zhu","year":"2016","unstructured":"Zhu P, et al. A simplified cuff technique for abdominal aortic transplantation in mice. J Surg Res. 2016;200(2):707\u201313.","journal-title":"J Surg Res"},{"key":"10495_CR3","first-page":"e50290","volume":"75","author":"L Qin","year":"2013","unstructured":"Qin L, Yu L, Min W. Mouse models for graft arteriosclerosis. J Vis Exp. 2013;75:e50290.","journal-title":"J Vis Exp"},{"issue":"1","key":"10495_CR4","doi-asserted-by":"publisher","first-page":"133","DOI":"10.1161\/01.ATV.14.1.133","volume":"14","author":"Y Nakashima","year":"1994","unstructured":"Nakashima Y, et al. ApoE-deficient mice develop lesions of all phases of atherosclerosis throughout the arterial tree. Arterioscler Thromb. 1994;14(1):133\u201340.","journal-title":"Arterioscler Thromb"},{"key":"10495_CR5","doi-asserted-by":"publisher","first-page":"860541","DOI":"10.3389\/fneur.2022.860541","volume":"13","author":"FX Liu","year":"2022","unstructured":"Liu FX, et al. Modified protocol for establishment of intracranial arterial dolichoectasia model by injection of elastase into cerebellomedullary cistern in mice. Front Neurol. 2022;13:860541.","journal-title":"Front Neurol"},{"key":"10495_CR6","doi-asserted-by":"crossref","unstructured":"Sawada H, et al. Ultrasound imaging of the thoracic and abdominal aorta in mice to determine aneurysm dimensions. J Vis Exp. 2019;(145):10.3791\/59013.","DOI":"10.3791\/59013-v"},{"key":"10495_CR7","doi-asserted-by":"crossref","unstructured":"Liao R, et al. Generation and characterization of right ventricular myocardial infarction induced by permanent ligation of the right coronary artery in mice. J Vis Exp. 2022;(180):10.3791\/63508.","DOI":"10.3791\/63508-v"},{"key":"10495_CR8","doi-asserted-by":"crossref","unstructured":"Wang Q, et al. Induction of right ventricular failure by pulmonary artery constriction and evaluation of right ventricular function in mice. J Vis Exp. 2019;(147):10.3791\/59431.","DOI":"10.3791\/59431"},{"key":"10495_CR9","doi-asserted-by":"crossref","unstructured":"Tang GL and Kim KJ. Laser Doppler perfusion imaging in the mouse hindlimb. J Vis Exp. 2021;(170):10.3791\/62012.","DOI":"10.3791\/62012"},{"key":"10495_CR10","doi-asserted-by":"publisher","first-page":"104499","DOI":"10.1016\/j.ebiom.2023.104499","volume":"90","author":"CY Wang","year":"2023","unstructured":"Wang CY, et al. Neutrophil extracellular traps as a unique target in the treatment of chemotherapy-induced peripheral neuropathy. EBioMedicine. 2023;90:104499.","journal-title":"EBioMedicine"},{"issue":"18","key":"10495_CR11","doi-asserted-by":"publisher","first-page":"1771","DOI":"10.1001\/jama.2022.5218","volume":"327","author":"L R\u00e4ber","year":"2022","unstructured":"R\u00e4ber L, et al. Effect of alirocumab added to high-intensity statin therapy on coronary atherosclerosis in patients with acute myocardial infarction: the PACMAN-AMI randomized clinical trial. JAMA. 2022;327(18):1771\u201381.","journal-title":"JAMA"},{"issue":"1","key":"10495_CR12","doi-asserted-by":"publisher","first-page":"138","DOI":"10.1016\/j.yexmp.2017.01.013","volume":"102","author":"DA Chistiakov","year":"2017","unstructured":"Chistiakov DA, et al. The phenomenon of atherosclerosis reversal and regression: lessons from animal models. Exp Mol Pathol. 2017;102(1):138\u201345.","journal-title":"Exp Mol Pathol"},{"key":"10495_CR13","doi-asserted-by":"publisher","first-page":"191","DOI":"10.1146\/annurev-med-042418-011108","volume":"71","author":"IJ Goldberg","year":"2020","unstructured":"Goldberg IJ, Sharma G, Fisher EA. Atherosclerosis: making a U turn. Annu Rev Med. 2020;71:191\u2013201.","journal-title":"Annu Rev Med"},{"issue":"6","key":"10495_CR14","doi-asserted-by":"publisher","first-page":"e0230649","DOI":"10.1371\/journal.pone.0230649","volume":"15","author":"H Dun","year":"2020","unstructured":"Dun H, et al. Combined abdominal heterotopic heart and aorta transplant model in mice. PLoS ONE. 2020;15(6):e0230649.","journal-title":"PLoS ONE"}],"container-title":["Journal of Cardiovascular Translational Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s12265-024-10495-w.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s12265-024-10495-w\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s12265-024-10495-w.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,9,3]],"date-time":"2024-09-03T15:34:35Z","timestamp":1725377675000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s12265-024-10495-w"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,2,26]]},"references-count":14,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2024,8]]}},"alternative-id":["10495"],"URL":"https:\/\/doi.org\/10.1007\/s12265-024-10495-w","relation":{},"ISSN":["1937-5387","1937-5395"],"issn-type":[{"value":"1937-5387","type":"print"},{"value":"1937-5395","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,2,26]]},"assertion":[{"value":"18 November 2023","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"2 February 2024","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"26 February 2024","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"No human studies were carried out by the authors for this article. All animal procedures in this study were conducted in accordance with the Guide for the Care and Use of Laboratory Animals published by the US National Institutes of Health (NIH Publication No. 85\u201323, revised in 1996) and approved by the Animal Ethics Committee of Nanfang Hospital, Southern Medical University (approval number: IACUC-LAC-20220830\u2013001).","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics Approval"}},{"value":"The authors declare no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of Interest"}}]}}