{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,10]],"date-time":"2026-04-10T19:59:26Z","timestamp":1775851166397,"version":"3.50.1"},"reference-count":38,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2012,4,6]],"date-time":"2012-04-06T00:00:00Z","timestamp":1333670400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Breast Cancer"],"published-print":{"date-parts":[[2014,1]]},"DOI":"10.1007\/s12282-012-0354-1","type":"journal-article","created":{"date-parts":[[2012,4,5]],"date-time":"2012-04-05T08:49:16Z","timestamp":1333615756000},"page":"66-74","source":"Crossref","is-referenced-by-count":57,"title":["Protein expression, gene amplification, and mutational analysis of EGFR in triple-negative breast cancer"],"prefix":"10.1007","volume":"21","author":[{"given":"Hiroki","family":"Nakajima","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuko","family":"Ishikawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mio","family":"Furuya","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takaaki","family":"Sano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshihiro","family":"Ohno","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Horiguchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tetsunari","family":"Oyama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2012,4,6]]},"reference":[{"key":"354_CR1","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1038\/nrclinonc.2010.154","volume":"7","author":"L Carey","year":"2010","unstructured":"Carey L, Winer E, Viale G, Cameron D, Gianni L. Triple-negative breast cancer: disease entity or title of convenience? Nat Rev Clin Oncol. 2010;7:683\u201392.","journal-title":"Nat Rev Clin Oncol"},{"key":"354_CR2","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1007\/s10549-008-0206-z","volume":"116","author":"G Viale","year":"2009","unstructured":"Viale G, Rotmensz N, Maisonneuve P, Bottiglieri L, Montagna E, Luini A, et al. Invasive ductal carcinoma of the breast with the \u201ctriple-negative\u201d phenotype: prognostic implications of EGFR immunoreactivity. Breast Cancer Res Treat. 2009;116:317\u201328.","journal-title":"Breast Cancer Res Treat"},{"key":"354_CR3","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/S0014-5793(97)00412-2","volume":"410","author":"I Alroy","year":"1997","unstructured":"Alroy I, Yarden Y. The ErbB signaling network in embryogenesis and oncogenesis: signal diversification through combinatorial ligand\u2013receptor interactions. FEBS Lett. 1997;410:83\u20136.","journal-title":"FEBS Lett"},{"key":"354_CR4","doi-asserted-by":"crossref","first-page":"897","DOI":"10.1006\/bbrc.1999.1144","volume":"261","author":"W Liu","year":"1999","unstructured":"Liu W, Li J, Roth RA. Heregulin regulation of Akt\/protein kinase B I breast cancer cells. Biochem Biophys Res Commun. 1999;261:897\u2013903.","journal-title":"Biochem Biophys Res Commun"},{"key":"354_CR5","doi-asserted-by":"crossref","first-page":"3291","DOI":"10.1038\/sj.onc.1210422","volume":"26","author":"PJ Roberts","year":"2007","unstructured":"Roberts PJ, Der CJ. Targeting the Raf-MEK-ERK mitogen-activated protein kinase cascade for the treatment of cancer. Oncogene. 2007;26:3291\u2013310.","journal-title":"Oncogene"},{"key":"354_CR6","doi-asserted-by":"crossref","first-page":"1263","DOI":"10.1016\/j.bbamcr.2006.10.001","volume":"1773","author":"JA McCubrey","year":"2007","unstructured":"McCubrey JA, Steelman LS, Chappel WH, Abrams SL, Wong EWT, Chang F, et al. Roles of the Raf\/MEK\/ERK pathway in cell growth, malignant transformation and drug resistance. Biochim Biophys Acta. 2007;1773:1263\u201384.","journal-title":"Biochim Biophys Acta"},{"key":"354_CR7","doi-asserted-by":"crossref","first-page":"7455","DOI":"10.1038\/sj.onc.1209085","volume":"24","author":"DA Altomare","year":"2005","unstructured":"Altomare DA, Testa JR. Perturbations of the AKT signaling pathway in human cancer. Oncogene. 2005;24:7455\u201364.","journal-title":"Oncogene"},{"key":"354_CR8","doi-asserted-by":"crossref","first-page":"2129","DOI":"10.1056\/NEJMoa040938","volume":"350","author":"TJ Lynch","year":"2004","unstructured":"Lynch TJ, Bell DW, Sordella R, Gurubhagavatula S, Okimoto RA, Brannigan BW, et al. Activating mutations in the epidermal growth factor receptor underlying responsiveness of non-small-cell lung cancer to gefitinib. N Engl J Med. 2004;350:2129\u201339.","journal-title":"N Engl J Med"},{"key":"354_CR9","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1093\/jnci\/dji112","volume":"97","author":"F Cappuzzo","year":"2005","unstructured":"Cappuzzo F, Hirsch FR, Rossi E, Bartolini S, Ceresoli GL, Bemis L, et al. Epidermal growth factor receptor gene and protein and gefitinib sensitivity in non-small-cell lung cancer. J Natl Cancer Inst. 2005;97:643\u201355.","journal-title":"J Natl Cancer Inst"},{"key":"354_CR10","doi-asserted-by":"crossref","first-page":"S14","DOI":"10.1038\/onc.2009.197","volume":"28","author":"T John","year":"2009","unstructured":"John T, Liu G, Tsao MS. Overview of molecular testing in non-small-cell lung cancer: mutational analysis, gene copy number, protein expression and other biomarkers of EGFR for the prediction of response to tyrosine kinase inhibitors. Oncogene. 2009;28:S14\u201323.","journal-title":"Oncogene"},{"key":"354_CR11","doi-asserted-by":"crossref","first-page":"627","DOI":"10.1007\/s10549-010-1293-1","volume":"125","author":"SK Pal","year":"2011","unstructured":"Pal SK, Childs BH, Pegram M. Triple negative breast cancer: unmet medical needs. Breast Cancer Res Treat. 2011;125:627\u201336. doi: 10.1007\/s10549-010-1293-1 .","journal-title":"Breast Cancer Res Treat"},{"key":"354_CR12","doi-asserted-by":"crossref","first-page":"1027","DOI":"10.1038\/modpathol.3800438","volume":"18","author":"R Bhargava","year":"2005","unstructured":"Bhargava R, Gerald WL, Li AR, Pan Q, Lal P, Ladanyi M, et al. EGFR gene amplification in breast cancer: correlation with epidermal growth factor receptor mRNA and protein expression and HER-2 status and absence of EGFR-activating mutations. Mod Pathol. 2005;18:1027\u201333.","journal-title":"Mod Pathol"},{"key":"354_CR13","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1002\/path.2004","volume":"209","author":"JS Reis-Filho","year":"2006","unstructured":"Reis-Filho JS, Pinheiro C, Lambros MB, Milanezi F, Carvalho S, Savage K, et al. EGFR amplification and lack of activating mutations in metaplastic breast carcinomas. J Pathol. 2006;209:445\u201353.","journal-title":"J Pathol"},{"key":"354_CR14","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1093\/annonc\/mdl322","volume":"18","author":"D Generali","year":"2007","unstructured":"Generali D, Leek R, Fox SB, Moore JW, Taylor C, Chambers P, et al. EGFR mutations in exons 18\u201321 in sporadic breast cancer. Ann Oncol. 2007;18:203\u20135.","journal-title":"Ann Oncol"},{"key":"354_CR15","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1186\/1471-2407-8-309","volume":"8","author":"T Toyama","year":"2008","unstructured":"Toyama T, Yamashita H, Kondo N, Okuda K, Takahashi S, Sasaki H, et al. Frequently increased epidermal growth factor receptor (EGFR) copy numbers and decreased BRCA1 mRNA expression in Japanese triple-negative breast cancers. BMC Cancer. 2008;8:309.","journal-title":"BMC Cancer"},{"key":"354_CR16","doi-asserted-by":"crossref","first-page":"R35","DOI":"10.1186\/bcr2857","volume":"13","author":"YH Teng","year":"2011","unstructured":"Teng YH, Tan WJ, Thike AA, Cheok PY, Tse GM, Wong NS, et al. Mutations in the epidermal growth factor receptor (EGFR) gene in triple negative breast cancer: possible implications for targeted therapy. Breast Cancer Res. 2011;13:R35. doi: 10.1186\/bcr2857 .","journal-title":"Breast Cancer Res"},{"key":"354_CR17","doi-asserted-by":"crossref","first-page":"582","DOI":"10.1038\/labinvest.3700077","volume":"84","author":"C Kersting","year":"2004","unstructured":"Kersting C, Tidow N, Schmidt H, Liedtke C, Neumann J, Boecker W, et al. Gene dosage PCR and fluorescence in situ hybridization reveal low frequency of egfr amplifications despite protein overexpression in invasive breast carcinoma. Lab Invest. 2004;84:582\u20137.","journal-title":"Lab Invest"},{"key":"354_CR18","doi-asserted-by":"crossref","first-page":"956","DOI":"10.1016\/j.ejso.2007.01.033","volume":"33","author":"K Park","year":"2007","unstructured":"Park K, Han S, Shin E, Kim HJ, Kim JY. EGFR gene and protein expression in breast cancers. Eur J Surg Oncol. 2007;33:956\u201360.","journal-title":"Eur J Surg Oncol"},{"key":"354_CR19","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1016\/j.cancergencyto.2010.07.118","volume":"203","author":"B Gumuskaya","year":"2010","unstructured":"Gumuskaya B, Alper M, Hucumenoglu S, Altundag K, Uner A, Guler G. EGFR expression and gene copy number in triple-negative breast carcinoma. Cancer Genet Cytogenet. 2010;203:222\u20139.","journal-title":"Cancer Genet Cytogenet"},{"key":"354_CR20","unstructured":"The Japanese Breast Cancer Society. Histological classification of breast tumor. In: General rules for clinical and pathological recording of breast cancer. 16th ed. Tokyo: Kanehara; 2008; p. 18\u201324."},{"key":"354_CR21","doi-asserted-by":"crossref","first-page":"656","DOI":"10.1111\/j.1349-7006.2011.01858.x","volume":"102","author":"Y Ishikawa","year":"2011","unstructured":"Ishikawa Y, Horiguchi J, Toya H, Nakajima H, Hayashi M, Tagaya N, et al. Triple-negative breast cancer: histological subtypes and immunohistochemical and clinicopathological features. Cancer Sci. 2011;102:656\u201362. doi: 10.1111\/j.1349-7006.2011.01858.x .","journal-title":"Cancer Sci"},{"key":"354_CR22","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1038\/modpathol.3800528","volume":"19","author":"CA Livasy","year":"2006","unstructured":"Livasy CA, Karaca G, Nanda R, Tretiakova MS, Olopade OI, Moore DT, et al. Phenotypic evaluation of the basal-like subtype of invasive breast carcinoma. Mod Pathol. 2006;19:264\u201371.","journal-title":"Mod Pathol"},{"key":"354_CR23","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1007\/s12282-009-0153-5","volume":"16","author":"Y Sasaki","year":"2009","unstructured":"Sasaki Y, Tsuda H. Clinicopathological characteristics of triple-negative breast cancers. Breast Cancer. 2009;16:254\u20139.","journal-title":"Breast Cancer"},{"key":"354_CR24","doi-asserted-by":"crossref","first-page":"1134","DOI":"10.1016\/S0046-8177(99)90028-X","volume":"30","author":"H Tsuda","year":"1999","unstructured":"Tsuda H, Takarabe T, Hasegawa T, Murata T, Hirohashi S. Myoepithelial differentiation in high-grade invasive ductal carcinomas with large central acellular zones. Hum Pathol. 1999;30:1134\u20139.","journal-title":"Hum Pathol"},{"key":"354_CR25","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1111\/j.1365-2559.2008.03075.x","volume":"53","author":"S Shousha","year":"2008","unstructured":"Shousha S. Oestrogen receptor status of breast carcinoma: Allred\/H score conversion table. Histopathology. 2008;53:346\u20137.","journal-title":"Histopathology"},{"key":"354_CR26","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1186\/1746-1596-3-41","volume":"3","author":"H Nitta","year":"2008","unstructured":"Nitta H, Hauss-Wegrzyniak B, Lehrkamp M, Murillo AE, Gaire F, Farrell M. Development of automated brightfield double in situ hybridization (BDISH) application for HER2 gene and chromosome 17 centromere (CEN 17) for breast carcinomas and an assay performance comparison to manual dual color HER2 fluorescence in situ hybridization (FISH). Diagn Pathol. 2008;3:41.","journal-title":"Diagn Pathol"},{"key":"354_CR27","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1038\/nmeth1007","volume":"4","author":"Y Mitani","year":"2007","unstructured":"Mitani Y, Lezhava A, Kawai Y, Kikuchi T, Oguchi-Katayama A, Kogo Y, et al. Rapid SNP diagnostics using asymmetric isothermal amplification and a new mismatch-suppression technology. Nat Methods. 2007;4:257\u201362.","journal-title":"Nat Methods"},{"key":"354_CR28","doi-asserted-by":"crossref","first-page":"4974","DOI":"10.1158\/1078-0432.CCR-07-0509","volume":"13","author":"K Hoshi","year":"2007","unstructured":"Hoshi K, Takakura H, Mitani Y, Tatsumi K, Momiyama N, Ichikawa Y, et al. Rapid detection of epidermal growth factor receptor mutations in lung cancer by the SMart-amplification process. Clin Cancer Res. 2007;13:4974\u201383.","journal-title":"Clin Cancer Res"},{"key":"354_CR29","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1016\/j.biologicals.2008.01.003","volume":"36","author":"Y Kawai","year":"2008","unstructured":"Kawai Y, Kikuchi T, Mitani Y, Kogo Y, Itoh M, Usui K, et al. Sensitive detection of EGFR mutations using a competitive probe to suppress background in the SMart amplification process. Biologicals. 2008;36:234\u20138.","journal-title":"Biologicals"},{"key":"354_CR30","doi-asserted-by":"crossref","first-page":"1234","DOI":"10.1002\/cncr.24816","volume":"116","author":"MF Rimawi","year":"2010","unstructured":"Rimawi MF, Shetty PB, Weiss HL, Schiff R, Osborne CK, Chamness GC, et al. Epidermal growth factor receptor expression in breast cancer association with biologic phenotype and clinical outcomes. Cancer. 2010;116:1234\u201342.","journal-title":"Cancer"},{"key":"354_CR31","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1097\/PDM.0b013e31816f6374","volume":"18","author":"GD Francis","year":"2009","unstructured":"Francis GD, Jones MA, Beadle GF, Stein SR. Bright-field in situ hybridization for HER2 gene amplification in breast cancer using tissue microarrays: correlation between chromogenic (CISH) and automated silver-enhanced (SISH) methods with patient outcome. Diagn Mol Pathol. 2009;18:88\u201395.","journal-title":"Diagn Mol Pathol"},{"key":"354_CR32","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1111\/j.1365-2559.2011.03894.x","volume":"59","author":"E Garc\u00eda-Garc\u00eda","year":"2011","unstructured":"Garc\u00eda-Garc\u00eda E, G\u00f3mez-Mart\u00edn C, Angulo B, Conde E, Su\u00e1rez-Gauthier A, Adrados M, et al. Hybridization for human epidermal growth factor receptor 2 testing in gastric carcinoma: a comparison of fluorescence in-situ hybridization with a novel fully automated dual-colour silver in-situ hybridization method. Histopathology. 2011;59:8\u201317. doi: 10.1111\/j.1365-2559.2011.03894.x .","journal-title":"Histopathology"},{"key":"354_CR33","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1002\/cncr.11398","volume":"99","author":"HJ Shin","year":"2003","unstructured":"Shin HJ, Shin DM, Tarco E, Sneige N. Detection of numerical aberrations of chromosomes 7 and 9 in cytologic specimens of pleural malignant mesothelioma. Cancer. 2003;99:233\u20139.","journal-title":"Cancer"},{"issue":"17","key":"354_CR34","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1158\/1078-0432.CCR-10-0208","volume":"15","author":"YH Li","year":"2011","unstructured":"Li YH, Wang F, Shen L, Deng YM, Shao Q, Feng F, et al. EGFR fluorescence in situ hybridization pattern of chromosome 7 disomy predicts resistance to cetuximab in KRAS wild-type metastatic colorectal cancer patients. Clin Cancer Res. 2011;15(17):382\u201390. doi: 10.1158\/1078-0432.CCR-10-0208 .","journal-title":"Clin Cancer Res"},{"key":"354_CR35","doi-asserted-by":"crossref","first-page":"414","DOI":"10.1097\/01.JTO.0000268675.02744.b0","volume":"2","author":"LE Buckingham","year":"2007","unstructured":"Buckingham LE, Coon JS, Morrison LE, Jacobson KK, Jewell SS, Kaiser KA, et al. The prognostic value of chromosome 7 polysomy in non-small cell lung cancer patients treated with gefitinib. J Thorac Oncol. 2007;2:414\u201322.","journal-title":"J Thorac Oncol"},{"key":"354_CR36","doi-asserted-by":"crossref","first-page":"2013","DOI":"10.1016\/j.cellsig.2007.06.023","volume":"19","author":"R Zandi","year":"2007","unstructured":"Zandi R, Larsen AB, Andersen P, Stockhausen MT, Poulsen HS. Mechanisms for oncogenic activation of the epidermal growth factor receptor. Cell Signal. 2007;19:2013\u201323.","journal-title":"Cell Signal"},{"key":"354_CR37","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1038\/modpathol.3800525","volume":"19","author":"S Bose","year":"2006","unstructured":"Bose S, Chandran S, Mirocha JM, Bose N. The Akt pathway in human breast cancer: a tissue-array-based analysis. Mod Pathol. 2006;19:238\u201345.","journal-title":"Mod Pathol"},{"key":"354_CR38","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1007\/s10549-010-1012-y","volume":"127","author":"MA Aleskandarany","year":"2011","unstructured":"Aleskandarany MA, Rakha EA, Ahmed MA, Powe DG, Ellis IO, Green AR. Clinicopathologic and molecular significance of phospho-Akt expression in early invasive breast cancer. Breast Cancer Res Treat. 2011;127:407\u201316. doi: 10.1007\/s10549-010-1012-y .","journal-title":"Breast Cancer Res Treat"}],"container-title":["Breast Cancer"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s12282-012-0354-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s12282-012-0354-1\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s12282-012-0354-1","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,6,3]],"date-time":"2019-06-03T12:40:49Z","timestamp":1559565649000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s12282-012-0354-1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,4,6]]},"references-count":38,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2014,1]]}},"alternative-id":["354"],"URL":"https:\/\/doi.org\/10.1007\/s12282-012-0354-1","relation":{},"ISSN":["1340-6868","1880-4233"],"issn-type":[{"value":"1340-6868","type":"print"},{"value":"1880-4233","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,4,6]]}}}