{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T15:59:34Z","timestamp":1786982374572,"version":"build-2736575974"},"reference-count":24,"publisher":"Springer Science and Business Media LLC","issue":"2","license":[{"start":{"date-parts":[[2024,4,4]],"date-time":"2024-04-04T00:00:00Z","timestamp":1712188800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2024,4,4]],"date-time":"2024-04-04T00:00:00Z","timestamp":1712188800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["J Hematopathol"],"published-print":{"date-parts":[[2024,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    ALK-positive (\u2009+) large B cell lymphoma (ALK\u2009+\u2009LBCL) is a rare distinct subtype of diffuse large B cell lymphoma presenting with high stage and aggressive behavior. Although B cell markers such as CD20, CD19, and CD22 are generally negative, plasmacytic markers including CD138, CD38, and MUM1 are positive. T cell markers are negative with rare exceptions. We report an unusual case of ALK1\u2009+\u2009LBCL in a 58-year-old man with partial expression of CD3 without other T cell antigen expression. The tissue was evaluated with flow cytometry, immunohistochemistry, fluorescent in situ hybridization, and gene rearrangement studies. Gene rearrangement studies for\n                    <jats:italic>IGH<\/jats:italic>\n                    and\n                    <jats:italic>TCR<\/jats:italic>\n                    gamma were performed. Flow cytometry did not demonstrate any abnormal lymphoid populations. Tissue sectioning shows a malignant plasmacytic large cell neoplasm which expresses CD45 but is negative for CD20, CD79a, and PAX5. Plasmacytic markers CD138 and MUM1 are positive with kappa light chain restriction. Strong granular cytoplasmic expression of ALK is present. FISH showing disrupted ALK supports the diagnosis while\n                    <jats:italic>MYC<\/jats:italic>\n                    ,\n                    <jats:italic>BCL6<\/jats:italic>\n                    , and\n                    <jats:italic>BCL2<\/jats:italic>\n                    are intact\n                    <jats:italic>.<\/jats:italic>\n                    Gene rearrangement studies show coexisting\n                    <jats:italic>IGH<\/jats:italic>\n                    and\n                    <jats:italic>TCR<\/jats:italic>\n                    gamma clones; however, the\n                    <jats:italic>TCR<\/jats:italic>\n                    peak was present within a polyclonal background suggesting the disputed cells are likely only a subset of the T cell population. ALK\u2009+\u2009LBCL can present with an ambiguous immunophenotype, which warrants the use of multiple B cell, T cell, and plasmacytic antibodies. CD3 expression in this entity is rare and of uncertain clinical significance, but warrants further study.\n                  <\/jats:p>","DOI":"10.1007\/s12308-024-00582-x","type":"journal-article","created":{"date-parts":[[2024,4,4]],"date-time":"2024-04-04T04:02:26Z","timestamp":1712203346000},"page":"109-114","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["ALK-positive large B-cell lymphoma (ALK\u2009+\u2009LBCL) with aberrant CD3 expression"],"prefix":"10.1007","volume":"17","author":[{"given":"Jess","family":"Baker","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara L.","family":"Zadeh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0014-6400","authenticated-orcid":false,"given":"Nadine S.","family":"Aguilera","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,4,4]]},"reference":[{"issue":"18","key":"582_CR1","doi-asserted-by":"publisher","first-page":"2175","DOI":"10.1038\/sj.onc.1201062","volume":"14","author":"SW Morris","year":"1997","unstructured":"Morris SW, Naeve C, Mathew P, James PL, Kirstein MN, Cui X et al (1997) ALK, the chromosome 2 gene locus altered by the t(2;5) in non-Hodgkin\u2019s lymphoma, encodes a novel neural receptor tyrosine kinase that is highly related to leukocyte tyrosine kinase (LTK). 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This is a retrospective study. For this type of study, formal consent is not required.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval"}},{"value":"This is a retrospective study. For this type of study, informed consent is not required.","order":5,"name":"Ethics","group":{"name":"EthicsHeading","label":"Informed consent"}},{"value":"This submission does not include patient identifying information. For this type of study, consent for publication is not required.","order":6,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}}]}}