{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,12,30]],"date-time":"2024-12-30T18:51:31Z","timestamp":1735584691449},"reference-count":41,"publisher":"Springer Science and Business Media LLC","issue":"4","license":[{"start":{"date-parts":[[2020,3,19]],"date-time":"2020-03-19T00:00:00Z","timestamp":1584576000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2020,3,19]],"date-time":"2020-03-19T00:00:00Z","timestamp":1584576000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"}],"funder":[{"name":"National Key Research and Development Program of China","award":["2016YFC0905100"],"award-info":[{"award-number":["2016YFC0905100"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["World J Pediatr"],"published-print":{"date-parts":[[2020,8]]},"DOI":"10.1007\/s12519-020-00349-1","type":"journal-article","created":{"date-parts":[[2020,3,19]],"date-time":"2020-03-19T14:04:04Z","timestamp":1584626644000},"page":"401-410","update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Identification of eight novel mutations in 11 Chinese\u00a0patients with maple syrup urine disease"],"prefix":"10.1007","volume":"16","author":[{"given":"Wei-Hua","family":"Sun","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bing-Bing","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ya-Qiong","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meng-Yuan","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xin-Ran","family":"Dong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yue-Ping","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Lu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ping","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bin","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong-Jiang","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wen-Hao","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2020,3,19]]},"reference":[{"key":"349_CR1","doi-asserted-by":"publisher","first-page":"121","DOI":"10.1007\/BF01800354","volume":"15","author":"E Treacy","year":"1992","unstructured":"Treacy E, Clow CL, Reade TR, Chitayat D, Mamer OA, Scriver CR. Maple syrup urine disease: interrelations between branched-chain amino-, oxo- and hydroxyacids; implications for treatment; associations with CNS dysmyelination. J Inherit Metab Dis. 1992;15:121\u201335.","journal-title":"J Inherit Metab Dis"},{"key":"349_CR2","doi-asserted-by":"publisher","first-page":"5","DOI":"10.1016\/j.neuint.2017.05.018","volume":"117","author":"A Ambrus","year":"2018","unstructured":"Ambrus A, Adam-Vizi V. Human dihydrolipoamide dehydrogenase (E3) deficiency: novel insights into the structural basis and molecular pathomechanism. Neurochem Int. 2018;117:5\u201314.","journal-title":"Neurochem Int"},{"key":"349_CR3","doi-asserted-by":"publisher","first-page":"462","DOI":"10.1542\/peds.14.5.462","volume":"14","author":"JH Menkes","year":"1954","unstructured":"Menkes JH, Hurst PL, Craig JM. A new syndrome: progressive familial infantile cerebral dysfunction associated with an unusual urinary substance. Pediatrics. 1954;14:462\u20137.","journal-title":"Pediatrics"},{"key":"349_CR4","doi-asserted-by":"publisher","first-page":"57","DOI":"10.2147\/TACG.S125962","volume":"10","author":"P Blackburn","year":"2017","unstructured":"Blackburn P, Gass J, Vairo FPE, Farnham KM, Atwal HK, Macklin S, et al. Maple syrup urine disease: mechanisms and management. Appl Clin Genet. 2017;10:57\u201366.","journal-title":"Appl Clin Genet"},{"key":"349_CR5","doi-asserted-by":"publisher","first-page":"232","DOI":"10.1086\/316950","volume":"68","author":"MM Nellis","year":"2001","unstructured":"Nellis MM, Danner DJ. Gene preference in maple syrup urine disease. Am J Hum Genet. 2001;68:232\u20137.","journal-title":"Am J Hum Genet"},{"key":"349_CR6","doi-asserted-by":"publisher","first-page":"855","DOI":"10.1007\/s10545-015-9814-z","volume":"38","author":"XL Wang","year":"2015","unstructured":"Wang XL, Li CJ, Xing Y, Yang YH, Jia JP. Hypervalinemia and hyperleucine\u2013isoleucinemia caused by mutations in the branched-chain-amino-acid aminotransferase gene. J Inherit Metab Dis. 2015;38:855\u201361.","journal-title":"J Inherit Metab Dis"},{"key":"349_CR7","doi-asserted-by":"publisher","first-page":"809","DOI":"10.1002\/jimd.12135","volume":"42","author":"I Knerr","year":"2019","unstructured":"Knerr I, Colombo R, Urquhart J, Morais A, Merinero B, Oyarzabal A, et al. Expanding the genetic and phenotypic spectrum of branched-chain amino acid transferase 2 deficiency. J Inherit Metab Dis. 2019;42:809\u201317.","journal-title":"J Inherit Metab Dis"},{"key":"349_CR8","doi-asserted-by":"publisher","first-page":"18","DOI":"10.1002\/ajmg.c.20003","volume":"121C","author":"EG Puffenberger","year":"2003","unstructured":"Puffenberger EG. Genetic heritage of the old order mennonites of Southeastern Pennsylvania. Am J Med Genet C Semin Med Genet. 2003;121C:18\u2013311.","journal-title":"Am J Med Genet C Semin Med Genet"},{"key":"349_CR9","doi-asserted-by":"publisher","first-page":"105","DOI":"10.1007\/s11011-016-9867-1","volume":"32","author":"M Abiri","year":"2017","unstructured":"Abiri M, Karamzadeh R, Mojbafan M, Alaei MR, Jodaki A, Safi M, et al. In silico analysis of novel mutations in maple syrup urine disease patients from Iran. Metab Brain Dis. 2017;32:105\u201313.","journal-title":"Metab Brain Dis"},{"key":"349_CR10","doi-asserted-by":"publisher","first-page":"1270","DOI":"10.1016\/j.cca.2011.03.028","volume":"412","author":"W Sun","year":"2011","unstructured":"Sun W, Wang Y, Yang Y, Wang J, Cao Y, Luo F, et al. The screening of inborn errors of metabolism in sick Chinese infants by tandem mass spectrometry and gas chromatography\/mass spectrometry. Clin Chim Acta. 2011;412:1270\u20134.","journal-title":"Clin Chim Acta"},{"key":"349_CR11","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1016\/j.gene.2019.04.086","volume":"710","author":"C Yang","year":"2019","unstructured":"Yang C, Linpeng S, Cao Y, Wu L. Identification of six novel mutations in five infants with suspected maple syrup urine disease based on blood and urine metabolism screening. Gene. 2019;710:9\u201316.","journal-title":"Gene"},{"key":"349_CR12","doi-asserted-by":"publisher","first-page":"617","DOI":"10.1016\/j.ejmg.2015.10.002","volume":"58","author":"XY Li","year":"2015","unstructured":"Li XY, Ding Y, Liu Y, Ma Y, Song J, Wang Q, et al. Eleven novel mutations of the BCKDHA, BCKDHB and DBT genes associated with maple syrup urine disease in the Chinese population: report on eight cases. Eur J Med Genet. 2015;58:617\u201323.","journal-title":"Eur J Med Genet"},{"key":"349_CR13","doi-asserted-by":"publisher","first-page":"741","DOI":"10.1007\/s11011-017-0168-0","volume":"33","author":"X Li","year":"2018","unstructured":"Li X, Yang Y, Gao Q, Gao M, Lv Y, Dong R, et al. Clinical characteristics and mutation analysis of five Chinese patients with maple syrup urine disease. Metab Brain Dis. 2018;33:741\u201351.","journal-title":"Metab Brain Dis"},{"key":"349_CR14","doi-asserted-by":"publisher","first-page":"765","DOI":"10.1007\/s11011-017-9959-6","volume":"32","author":"L Su","year":"2017","unstructured":"Su L, Lu Z, Li F, Shao Y, Sheng H, Cai Y, et al. Two homozygous mutations in the exon 5 of BCKDHB gene that may cause the classic form of maple syrup urine disease. Metab Brain Dis. 2017;32:765\u201372.","journal-title":"Metab Brain Dis"},{"key":"349_CR15","doi-asserted-by":"publisher","first-page":"515","DOI":"10.1016\/j.pedneo.2018.01.006","volume":"59","author":"B Han","year":"2018","unstructured":"Han B, Han B, Guo B, Liu Y, Cao Z. Two novel mutations in the BCKDHB gene that cause maple syrup urine disease. Pediatr Neonatol. 2018;59:515\u20139.","journal-title":"Pediatr Neonatol"},{"key":"349_CR16","doi-asserted-by":"publisher","first-page":"145","DOI":"10.3389\/fgene.2018.00145","volume":"9","author":"G Liu","year":"2018","unstructured":"Liu G, Ma D, Hu P, Wang W, Luo C, Wang Y, et al. A novel whole gene deletion of BCKDHB by alu-mediated non-allelic recombination in a Chinese patient with maple syrup urine disease. Front Genet. 2018;9:145\u201354.","journal-title":"Front Genet"},{"key":"349_CR17","first-page":"4872","volume":"19","author":"Y Liu","year":"2019","unstructured":"Liu Y, Chu X, Liu R, Sun Y, Kong Q, Li Q. Paroxysmal spasticity of lower extremities as the initial symptom in two siblings with maple syrup urine disease. Mol Med Rep. 2019;19:4872\u201380.","journal-title":"Mol Med Rep"},{"key":"349_CR18","doi-asserted-by":"publisher","first-page":"679","DOI":"10.1203\/00006450-197406000-00009","volume":"8","author":"DM Cox","year":"1974","unstructured":"Cox DM, Niewczas-Late V, Riffell MI, Hamerton JL. Chromosomal mosaicism in diagnostic amniotic fluid cell cultures. Pediatr Res. 1974;8:679\u201383.","journal-title":"Pediatr Res"},{"key":"349_CR19","doi-asserted-by":"publisher","first-page":"D313","DOI":"10.1093\/nar\/gkw1132","volume":"45","author":"S Bienert","year":"2017","unstructured":"Bienert S, Waterhouse A, de Beer TA, Tauriello G, Studer G, Bordoli L, et al. The SWISS-MODEL repository-new features and functionality. Nucleic Acids Res. 2017;45:D313\u20139.","journal-title":"Nucleic Acids Res"},{"key":"349_CR20","doi-asserted-by":"publisher","first-page":"W296","DOI":"10.1093\/nar\/gky427","volume":"46","author":"A Waterhouse","year":"2018","unstructured":"Waterhouse A, Bertoni M, Bienert S, Studer G, Tauriello G, Gumienny R, et al. SWISS-MODEL: homology modelling of protein structures and complexes. Nucleic Acids Res. 2018;46:W296\u2013303.","journal-title":"Nucleic Acids Res"},{"key":"349_CR21","doi-asserted-by":"publisher","first-page":"5983","DOI":"10.1038\/sj.emboj.7601444","volume":"25","author":"M Kato","year":"2006","unstructured":"Kato M, Wynn RM, Chuang JL, Brautigam CA, Custorio M, Chuang DT. A synchronized substrate-gating mechanism revealed by cubic-core structure of the bovine branched-chain \u03b1-ketoacid dehydrogenase complex. EMBO J. 2006;25:5983\u201394.","journal-title":"EMBO J"},{"key":"349_CR22","doi-asserted-by":"publisher","first-page":"287","DOI":"10.1016\/j.str.2005.10.009","volume":"14","author":"M Machius","year":"2006","unstructured":"Machius M, Wynn RM, Chuang JL, Li J, Kluger R, Yu D, et al. A versatile conformational switch regulates reactivity in human branched-chain \u03b1-ketoacid dehydrogenase. Structure. 2006;14:287\u201398.","journal-title":"Structure"},{"key":"349_CR23","doi-asserted-by":"publisher","first-page":"412","DOI":"10.1016\/j.ymgme.2012.05.023","volume":"106","author":"N Yang","year":"2012","unstructured":"Yang N, Han L, Gu X, Ye J, Qiu W, Zhang H, et al. Analysis of gene mutations in Chinese patients with maple syrup urine disease. Mol Genet Metab. 2012;106:412\u20138.","journal-title":"Mol Genet Metab"},{"key":"349_CR24","first-page":"297","volume":"55","author":"JL Chuang","year":"1994","unstructured":"Chuang JL, Fisher CR, Cox RP, Chuang DT. Molecular basis of maple syrup urine disease: novel mutations at the E1 alpha locus that impair E1(alpha 2 beta 2) assembly or decrease steady-state E1 alpha mRNA levels of branched-chain alpha-keto acid dehydrogenase complex. Am J Hum Genet. 1994;55:297\u2013304.","journal-title":"Am J Hum Genet"},{"key":"349_CR25","doi-asserted-by":"publisher","first-page":"89","DOI":"10.1023\/A:1015668425004","volume":"25","author":"A Dursun","year":"2002","unstructured":"Dursun A, Henneke M, Ozgul K, Gartner J, Co\u015fkun T, Tokatli A, et al. Maple syrup urine disease: mutation analysis in Turkish patients. J Inherit Metab Dis. 2002;25:89\u201397.","journal-title":"J Inherit Metab Dis"},{"key":"349_CR26","first-page":"167","volume":"41","author":"HD Park","year":"2011","unstructured":"Park HD, Lee DH, Hong YH, Kang DH, Lee YK, Song J, et al. Three Korean patients with maple syrup urine disease: four novel mutations in the BCKDHA gene. Ann Clin Lab Sci. 2011;41:167\u201373.","journal-title":"Ann Clin Lab Sci"},{"key":"349_CR27","doi-asserted-by":"publisher","first-page":"417","DOI":"10.1002\/humu.9187","volume":"22","author":"M Henneke","year":"2003","unstructured":"Henneke M, Flaschker N, Helbling C, M\u00fcller M, Schadewaldt P, G\u00e4rtner J, et al. Identification of twelve novel mutations in patients with classic and variant forms of maple syrup urine disease. Hum Mutat. 2003;22:417\u201324.","journal-title":"Hum Mutat"},{"key":"349_CR28","doi-asserted-by":"publisher","first-page":"903","DOI":"10.1007\/s10545-007-0579-x","volume":"30","author":"N Flaschker","year":"2007","unstructured":"Flaschker N, Feyen O, Fend S, Simon E, Schadewaldt P, Wendel U. Description of the mutations in 15 subjects with variant forms of maple syrup urine disease. J Inherit Metab Dis. 2007;30:903\u20139.","journal-title":"J Inherit Metab Dis"},{"key":"349_CR29","doi-asserted-by":"publisher","first-page":"17","DOI":"10.1016\/j.ymgmr.2017.03.006","volume":"11","author":"F Imtiaz","year":"2017","unstructured":"Imtiaz F, Al-Mostafa A, Allam R, Ramzan K, Al-Tassan N, Tahir AI, et al. Twenty novel mutations in BCKDHA, BCKDHB and DBT genes in a cohort of 52 Saudi Arabian patients with maple syrup urine disease. Mol Genet Metab Rep. 2017;11:17\u201323.","journal-title":"Mol Genet Metab Rep"},{"key":"349_CR30","first-page":"414","volume":"52","author":"CW Fisher","year":"1993","unstructured":"Fisher CW, Fisher CR, Chuang JL, Lau KS, Chuang DT, Cox RP. Occurrence of a 2-bp (AT) deletion allele and a nonsense (G-to-T) mutant allele at the E2 (DBT) locus of six patients with maple syrup urine disease: multiple-exon skipping as a secondary effect of the mutations. Am J Hum Genet. 1993;52:414\u201324.","journal-title":"Am J Hum Genet"},{"key":"349_CR31","doi-asserted-by":"publisher","first-page":"631","DOI":"10.1007\/s10545-018-0188-x","volume":"41","author":"G Scaini","year":"2018","unstructured":"Scaini G, Tonon T, Moura de Souza CF, Schuck PF, Ferreira GC, Quevedo J, et al. Evaluation of plasma biomarkers of inflammation in patients with maple syrup urine disease. J Inherit Metab Dis. 2018;41:631\u201340.","journal-title":"J Inherit Metab Dis"},{"key":"349_CR32","doi-asserted-by":"publisher","first-page":"548","DOI":"10.1186\/1471-2105-11-548","volume":"11","author":"H Venselaar","year":"2010","unstructured":"Venselaar H, Te Beek TA, Kuipers RK, Hekkelman ML, Vriend G. Protein structure analysis of mutations causing inheritable diseases. An e-Science approach with life scientist friendly interfaces. BMC Bioinform. 2010;11:548\u201357.","journal-title":"BMC Bioinform"},{"key":"349_CR33","doi-asserted-by":"publisher","first-page":"783","DOI":"10.1007\/s10545-017-0083-x","volume":"40","author":"MT Abi-Ward\u00e9","year":"2017","unstructured":"Abi-Ward\u00e9 MT, Roda C, Arnoux JB, Servais A, Habarou F, Brassier A, et al. Long-term metabolic follow-up and clinical outcome of 35 patients with maple syrup urine disease. J Inherit Metab Dis. 2017;40:783\u201392.","journal-title":"J Inherit Metab Dis"},{"key":"349_CR34","doi-asserted-by":"publisher","first-page":"377","DOI":"10.1007\/s10545-017-0033-7","volume":"40","author":"J Bouchereau","year":"2017","unstructured":"Bouchereau J, Leduc-Leballeur J, Pichard S, Imbard A, Benoist JF, Abi Warde MT, et al. Neurocognitive profiles in MSUD school-age patients. J Inherit Metab Dis. 2017;40:377\u201383.","journal-title":"J Inherit Metab Dis"},{"key":"349_CR35","doi-asserted-by":"publisher","first-page":"939","DOI":"10.1007\/s10545-012-9570-2","volume":"36","author":"A Servais","year":"2013","unstructured":"Servais A, Arnoux JB, Lamy C, Hummel A, Vittoz N, Katerinis I, et al. Treatment of acute decompensation of maple syrup urine disease in adult patients with a new parenteral amino-acid mixture. J Inherit Metab Dis. 2013;36:939\u201344.","journal-title":"J Inherit Metab Dis"},{"key":"349_CR36","doi-asserted-by":"publisher","first-page":"281","DOI":"10.1007\/s10545-008-0859-0","volume":"31","author":"JY Lee","year":"2008","unstructured":"Lee JY, Chiong MA, Estrada SC, Cutiongco-De la Paz EM, Silao CLT, Padilla CD. Maple syrup urine disease (MSUD)-clinical profile of 47 Filipino patients. J Inherit Metab Dis. 2008;31:281\u20135.","journal-title":"J Inherit Metab Dis"},{"key":"349_CR37","doi-asserted-by":"publisher","first-page":"614","DOI":"10.1038\/s41586-019-1503-x","volume":"572","author":"T Yoneshiro","year":"2019","unstructured":"Yoneshiro T, Wang Q, Tajima K, Matsushita M, Maki H, Igarashi K, et al. BCAA catabolism in brown fat controls energy homeostasis through SLC25A44. Nature. 2019;572:614\u20139.","journal-title":"Nature"},{"key":"349_CR38","doi-asserted-by":"publisher","first-page":"723","DOI":"10.1038\/nrendo.2014.171","volume":"10","author":"CJ Lynch","year":"2014","unstructured":"Lynch CJ, Adams SH. Branched-chain amino acids in metabolic signalling and insulin resistance. Nat Rev Endocrinol. 2014;10:723\u201336.","journal-title":"Nat Rev Endocrinol"},{"key":"349_CR39","doi-asserted-by":"publisher","first-page":"210","DOI":"10.1016\/j.ymgme.2014.05.006","volume":"112","author":"DM Frazier","year":"2014","unstructured":"Frazier DM, Allgeier C, Homer C, Marriage BJ, Ogata B, Rohr F, et al. Nutrition management guideline for maple syrup urine disease: an evidence- and consensus-based approach. Mol Genet Metab. 2014;112:210\u20137.","journal-title":"Mol Genet Metab"},{"key":"349_CR40","doi-asserted-by":"publisher","first-page":"707","DOI":"10.1111\/petr.12738","volume":"20","author":"T Yasui","year":"2016","unstructured":"Yasui T, Suzuki T, Hara F, Watanabe S, Uga N, Naoe A, et al. Successful living donor liver transplantation for classical maple syrup urine disease. Pediatr Transpl. 2016;20:707\u201310.","journal-title":"Pediatr Transpl"},{"key":"349_CR41","doi-asserted-by":"publisher","first-page":"22","DOI":"10.1016\/j.ymgmr.2018.08.006","volume":"17","author":"EZ Ali","year":"2018","unstructured":"Ali EZ, Ngu LH. Fourteen new mutations of BCKDHA, BCKDHB and DBT genes associated with maple syrup urine disease (MSUD) in Malaysian population. Mol Genet Metab Rep. 2018;17:22\u201330.","journal-title":"Mol Genet Metab Rep"}],"container-title":["World Journal of Pediatrics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s12519-020-00349-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s12519-020-00349-1\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s12519-020-00349-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,16]],"date-time":"2022-04-16T15:44:27Z","timestamp":1650123867000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s12519-020-00349-1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,3,19]]},"references-count":41,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2020,8]]}},"alternative-id":["349"],"URL":"https:\/\/doi.org\/10.1007\/s12519-020-00349-1","relation":{},"ISSN":["1708-8569","1867-0687"],"issn-type":[{"value":"1708-8569","type":"print"},{"value":"1867-0687","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,3,19]]},"assertion":[{"value":"24 July 2019","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"18 February 2020","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"19 March 2020","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Compliance with ethical standards"}},{"value":"This study was approved by the ethics committee of Children\u2019s Hospital, Fudan University. Informed consent for publication\u00a0was obtained from all of the families of individual participants included in the study.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval"}},{"value":"The authors have no conflicts of interest to declare.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"All guardians of the subjects included in this study provided appropriate informed consent.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Informed consent"}}]}}