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A total of 92 wheat grain and 79 cattle feed samples from different feed and food Brazilian industries were analyzed by ultra-high-performance liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS\/MS) between January 2023 and March 2025. Approximately 78% of wheat samples and 72% of cattle feed samples contained at least one EA. Ergosine was the predominant alkaloid in wheat, with a mean concentration of 8.06\u00a0\u00b5g\/kg. In contrast, ergotaminine was the primary contaminant in cattle feed, with a mean concentration of 2.37\u00a0\u00b5g\/kg. DON also showed high contamination levels, with mean concentrations of 1,913\u00a0\u00b5g\/kg in wheat and 222.14\u00a0\u00b5g\/kg in cattle feed. All five EAs were simultaneously detected in 8\/92 wheat samples, whereas no simultaneous occurrence of all five EAs was observed in cattle feed samples. The co-occurrence of DON and different EAs ranged from 14.1 to 65.2% in wheat and from 6.3 to 40.5% in cattle feed. These findings highlight the importance of mycotoxin monitoring in Brazil and demonstrate the robustness of UHPLC-MS\/MS as a tool for detecting these mycotoxins in complex matrices such as animal feed.<\/jats:p>","DOI":"10.1007\/s12550-026-00675-9","type":"journal-article","created":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T13:54:58Z","timestamp":1787925298000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Occurrence of ergot alkaloids and deoxynivalenol in Brazilian wheat and cattle feed determined by UHPLC-MS\/MS"],"prefix":"10.1007","volume":"42","author":[{"given":"Cristiane","family":"Rosa da Silva","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina","family":"Tonial Sim\u00f5es","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luara Medianeira","family":"de Lima Schl\u00f6sser","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isadora","family":"Fabris Laber","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luriane Medianeira","family":"Carossi Leal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Bonai Casal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos Alberto","family":"Ara\u00fajo de Almeida","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2376-2705","authenticated-orcid":false,"given":"Carlos Augusto","family":"Mallmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,8,28]]},"reference":[{"key":"675_CR1","doi-asserted-by":"publisher","first-page":"133902","DOI":"10.1016\/j.foodchem.2022.133902","volume":"399","author":"DCP Abreu","year":"2023","unstructured":"Abreu DCP, Vargas EA, Oliveira FAS, Madureira FD, Gomes MB, Bazzana MJF, Saczk AA (2023a) Validation and estimation of uncertainty of an LC-MS\/MS method for the simultaneous determination of 34 mycotoxins in cocoa beans. 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