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Norovirus-infected patients can produce aerosolized droplets which play a role in gastroenteritis transmission. The study aimed to assess bioaerosol sampling in combination with a virus concentrating procedure to facilitate molecular detection of norovirus genogroup (G) II from experimentally contaminated aerosols.\u00a0Using a nebulizer within an experimental chamber, aerosols of norovirus GII were generated at known concentrations. Air samples were then collected in both 5\u00a0mL and 20\u00a0mL water using the SKC BioSampler at a flow rate of 12.5\u00a0L\/min, 15\u00a0min. Subsequently, the virus in collected water was concentrated using speedVac centrifugation and quantified by RT-qPCR. The optimal distances between the nebulizer and the SKC BioSampler yielded high recoveries of the virus for both 5 and 20\u00a0mL collections. Following nebulization, norovirus GII RNA was detectable up to 120\u00a0min in 5\u00a0mL and up to 240\u00a0min in 20\u00a0mL collection. The concentrations of norovirus GII RNA recovered from air samples in the aerosol chamber ranged from 10<jats:sup>2<\/jats:sup> to 10<jats:sup>5<\/jats:sup> genome copies\/mL, with average recoveries of 25\u2009\u00b1\u200912% for 5\u00a0mL and 22\u2009\u00b1\u200919% for 20\u00a0mL collections. These findings provide quantitative data on norovirus GII in aerosols and introduce a novel virus concentrating method for aerosol collection in water, thus enhancing surveillance of this virus.<\/jats:p>","DOI":"10.1007\/s12560-024-09590-7","type":"journal-article","created":{"date-parts":[[2024,3,21]],"date-time":"2024-03-21T14:02:54Z","timestamp":1711029774000},"page":"216-224","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Recovery and Quantification of Norovirus in Air Samples from Experimentally Produced Aerosols"],"prefix":"10.1007","volume":"16","author":[{"given":"Kitwadee","family":"Rupprom","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuwanda","family":"Thongpanich","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Woravat","family":"Sukkham","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fuangfa","family":"Utrarachkij","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3519-7862","authenticated-orcid":false,"given":"Leera","family":"Kittigul","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2024,3,21]]},"reference":[{"issue":"10","key":"9590_CR1","doi-asserted-by":"publisher","first-page":"2023","DOI":"10.1093\/cid\/ciz584","volume":"70","author":"M Alsved","year":"2020","unstructured":"Alsved, M., Fraenkel, C. 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