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Claude Gillot revived the approach for the study of the venous system by combining green\u2011latex infusion with post\u2011dissection vein painting (\u201ccolored segmentation\u201d) to enhance anatomical education. To detail Gillot\u2019s injection technique, evaluate its technical reliability, 400 fresh lower limbs (200 donors, mean age 75\u00a0years; Centre du Don des Corps, Paris) were irrigated with warm soapy water and injected via an ankle 19\u2011G butterfly into the great saphenous vein with filtered green latex (120\u2013150\u00a0ml; 20\u00a0ml syringe; 20\u201330\u00a0kPa). Proximal femoral venous drainage prevented reflux. After 24\u00a0h polymerization the limbs were dissected; venous segments were painted according to a seven\u2011color palette. Patency, leakage and dissection time were recorded. Three exemplary specimens were photogrammetrically documented. Overall venous patency reached 93% with minimal segmental leakage (mean\u2009&lt;\u20092\u00a0cm per limb). Dissection time per lower limb averaged ten\u202fhours. Gillot\u2019s colored\u2011segmentation protocol provides a vivid, dependable and inexpensive platform for teaching and research in venous anatomy. Its flexibility and compatibility with digital capture surpass many contemporary embalming or silicone\u2011based perfusion techniques. Future work should integrate three\u2011dimensional models into virtual\u2011reality curricula and quantify learning outcomes.<\/jats:p>","DOI":"10.1007\/s12565-025-00907-5","type":"journal-article","created":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T10:39:09Z","timestamp":1762425549000},"page":"102-107","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["The anatomical technique of injection, dissection and colored segmentation of the venous system: Claude Gillot\u2019s coloring technique"],"prefix":"10.1007","volume":"101","author":[{"given":"Jean Francois","family":"Uhl","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claude","family":"Gillot","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 Ramon Mogorron","family":"Huerta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manuel","family":"De Jesus Encarnacion Ramirez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gervith Reyes","family":"Soto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos Castillo","family":"Rangel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vladimir","family":"Nikolenko","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3686-8907","authenticated-orcid":false,"given":"Nicola","family":"Montemurro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,11,6]]},"reference":[{"key":"907_CR1","doi-asserted-by":"publisher","DOI":"10.1049\/htl2.12116","volume":"12","author":"M Autelitano","year":"2024","unstructured":"Autelitano M et al (2024) Augmented reality for rhinoplasty: 3D scanning and projected AR for intraoperative planning validation. 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