{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T22:00:49Z","timestamp":1782943249276,"version":"3.54.5"},"reference-count":32,"publisher":"Springer Science and Business Media LLC","issue":"2","license":[{"start":{"date-parts":[[2019,7,30]],"date-time":"2019-07-30T00:00:00Z","timestamp":1564444800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2019,7,30]],"date-time":"2019-07-30T00:00:00Z","timestamp":1564444800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Probiotics &amp; Antimicro. Prot."],"published-print":{"date-parts":[[2020,6]]},"DOI":"10.1007\/s12602-019-09573-2","type":"journal-article","created":{"date-parts":[[2019,7,30]],"date-time":"2019-07-30T17:02:31Z","timestamp":1564506151000},"page":"697-704","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Influence of Heterologously Expressed azurin from Pseudomonas aeruginosa on the Adhesion and Invasion of Pathogenic Bacteria to the Caco-2 Cell Line"],"prefix":"10.1007","volume":"12","author":[{"given":"Hajar Mohammadi","family":"Barzelighi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bahram Nasr","family":"Esfahani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bita","family":"Bakhshi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bahram","family":"Daraei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sharareh","family":"Moghim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hossein","family":"Fazeli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2019,7,30]]},"reference":[{"key":"9573_CR1","doi-asserted-by":"publisher","first-page":"1009","DOI":"10.1053\/j.gastro.2007.01.049","volume":"132","author":"V Necchi","year":"2007","unstructured":"Necchi V, Candusso ME, Tava F, Luinetti O, Ventura U, Fiocca R, Ricci V, Solcia E (2007) Intracellular, intercellular, and stromal invasion of gastric mucosa, preneoplastic lesions, and cancer by Helicobacter pylori. Gastroenterology 132:1009\u20131023. \nhttps:\/\/doi.org\/10.1053\/j.gastro.2007.01.049","journal-title":"Gastroenterology"},{"key":"9573_CR2","doi-asserted-by":"publisher","first-page":"2572","DOI":"10.1128\/JB.00017-16","volume":"198","author":"D Pletzer","year":"2016","unstructured":"Pletzer D, Hancock REW (2016) Antibiofilm peptides: potential as broad-spectrum agents. J Bacteriol 198:2572\u20132578. \nhttps:\/\/doi.org\/10.1128\/JB.00017-16","journal-title":"J Bacteriol"},{"key":"9573_CR3","doi-asserted-by":"publisher","first-page":"181","DOI":"10.1016\/S0928-8244(03)00228-1","volume":"38","author":"I Ofek","year":"2003","unstructured":"Ofek I, Hasty DL, Sharon N (2003) Anti-adhesion therapy of bacterial diseases: prospects and problems. FEMS Immunol Med Microbiol 38:181\u2013191. \nhttps:\/\/doi.org\/10.1016\/S0928-8244(03)00228-1","journal-title":"FEMS Immunol Med Microbiol"},{"key":"9573_CR4","doi-asserted-by":"publisher","first-page":"1457","DOI":"10.1586\/eri.12.145","volume":"10","author":"D Cozens","year":"2012","unstructured":"Cozens D, Read RC (2012) Anti-adhesion methods as novel therapeutics for bacterial infections. Expert Rev Anti-Infect Ther 10:1457\u20131468. \nhttps:\/\/doi.org\/10.1586\/eri.12.145","journal-title":"Expert Rev Anti-Infect Ther"},{"key":"9573_CR5","doi-asserted-by":"publisher","first-page":"2367","DOI":"10.1038\/sj.onc.1207376","volume":"23","author":"V Punj","year":"2004","unstructured":"Punj V, Bhattacharyya S, Saint-Dic D, Vasu C, Cunningham EA, Graves J, Yamada T, Constantinou AI, Christov K, White B, Li G, Majumdar D, Chakrabarty AM, Das Gupta TK (2004) Bacterial cupredoxin azurin as an inducer of apoptosis and regression in human breast cancer. Oncogene 23:2367\u20132378. \nhttps:\/\/doi.org\/10.1038\/sj.onc.1207376","journal-title":"Oncogene"},{"key":"9573_CR6","doi-asserted-by":"publisher","first-page":"1","DOI":"10.9734\/JAMPS\/2016\/20557","volume":"5","author":"MC Sereena","year":"2016","unstructured":"Sereena MC, Sebastian D (2016) Molecular detection of azurin: a powerful anticancer protein from native Pseudomonas isolates. J Adv Med Pharm Sci 5:1\u20137. \nhttps:\/\/doi.org\/10.9734\/JAMPS\/2016\/20557","journal-title":"J Adv Med Pharm Sci"},{"key":"9573_CR7","doi-asserted-by":"publisher","first-page":"1642","DOI":"10.4161\/cc.5.15.2992","volume":"5","author":"A Chaudhari","year":"2006","unstructured":"Chaudhari A, Fialho AM, Ratner D, Gupta P, Hong CS, Kahali S, Yamada T, Haldar K, Murphy S, Cho W, Chauhan VS, Das Gupta TK, Chakrabarty AM (2006) Azurin, Plasmodium falciparum malaria and HIV\/AIDS: inhibition of parasitic and viral growth by azurin. Cell Cycle 5:1642\u20131648. \nhttps:\/\/doi.org\/10.4161\/cc.5.15.2992","journal-title":"Cell Cycle"},{"key":"9573_CR8","doi-asserted-by":"publisher","unstructured":"Bernardes N, Ribeiro AS, Seruca R, et al (2011) Bacterial protein azurin as a new candidate drug to treat untreatable breast cancers. In: 1st Portuguese Biomedical Engineering Meeting. IEEE, pp 1\u20134. \nhttps:\/\/doi.org\/10.1109\/ENBENG.2011.6026047","DOI":"10.1109\/ENBENG.2011.6026047"},{"key":"9573_CR9","doi-asserted-by":"publisher","first-page":"178","DOI":"10.4161\/bbug.1.3.10903","volume":"1","author":"N Bernardes","year":"2010","unstructured":"Bernardes N, Seruca R, Chakrabarty AM, Fialho AM (2010) Microbial-based therapy of cancer: current progress and future prospects. Bioeng Bugs 1:178\u2013190. \nhttps:\/\/doi.org\/10.4161\/bbug.1.3.10903","journal-title":"Bioeng Bugs"},{"key":"9573_CR10","doi-asserted-by":"publisher","first-page":"1418","DOI":"10.1111\/j.1462-5822.2005.00567.x","volume":"7","author":"T Yamada","year":"2005","unstructured":"Yamada T, Fialho AM, Punj V, Bratescu L, Gupta TKD, Chakrabarty AM (2005) Internalization of bacterial redox protein azurin in mammalian cells: entry domain and specificity. Cell Microbiol 7:1418\u20131431. \nhttps:\/\/doi.org\/10.1111\/j.1462-5822.2005.00567.x","journal-title":"Cell Microbiol"},{"key":"9573_CR11","doi-asserted-by":"publisher","first-page":"14098","DOI":"10.1073\/pnas.222539699","volume":"99","author":"T Yamada","year":"2002","unstructured":"Yamada T, Goto M, Punj V, Zaborina O, Chen ML, Kimbara K, Majumdar D, Cunningham E, Das Gupta TK, Chakrabarty AM (2002) Bacterial redox protein azurin, tumor suppressor protein p53, and regression of cancer. Proc Natl Acad Sci U S A 99:14098\u201314103. \nhttps:\/\/doi.org\/10.1073\/pnas.222539699","journal-title":"Proc Natl Acad Sci U S A"},{"key":"9573_CR12","doi-asserted-by":"publisher","unstructured":"Fialho AM, Chakrabarty AM (2010) Promiscuous anticancer drugs from pathogenic bacteria: rational versus intelligent drug design. In: emerging cancer therapy. John Wiley & Sons, Inc., Hoboken, NJ, USA, pp 179\u2013198. \nhttps:\/\/doi.org\/10.1002\/9780470626528.ch8","DOI":"10.1002\/9780470626528.ch8"},{"key":"9573_CR13","doi-asserted-by":"publisher","first-page":"3011","DOI":"10.2147\/IJN.S26155","volume":"6","author":"AR Bizzarri","year":"2011","unstructured":"Bizzarri AR, Santini S, Coppari E et al (2011) Interaction of an anticancer peptide fragment of azurin with p53 and its isolated domains studied by atomic force spectroscopy. Int J Nanomedicine 6:3011\u20133019. \nhttps:\/\/doi.org\/10.2147\/IJN.S26155","journal-title":"Int J Nanomedicine"},{"key":"9573_CR14","doi-asserted-by":"publisher","first-page":"402","DOI":"10.1128\/AAC.01005-07","volume":"52","author":"A Naguleswaran","year":"2008","unstructured":"Naguleswaran A, Fialho AM, Chaudhari A, Hong CS, Chakrabarty AM, Sullivan WJ (2008) Azurin-like protein blocks invasion of Toxoplasma gondii through potential interactions with parasite surface antigen SAG1. Antimicrob Agents Chemother 52:402\u2013408. \nhttps:\/\/doi.org\/10.1128\/AAC.01005-07","journal-title":"Antimicrob Agents Chemother"},{"key":"9573_CR15","doi-asserted-by":"publisher","first-page":"195","DOI":"10.1111\/j.1432-1033.1989.tb14540.x","volume":"179","author":"RHA Arvidsson","year":"1989","unstructured":"Arvidsson RHA, Nordling M, Lundberg LG (1989) The azurin gene from Pseudomonas aeruginosa. Cloning and characterization. Eur J Biochem 179:195\u2013200. \nhttps:\/\/doi.org\/10.1111\/j.1432-1033.1989.tb14540.x","journal-title":"Eur J Biochem"},{"key":"9573_CR16","doi-asserted-by":"publisher","first-page":"211","DOI":"10.1016\/0014-5793(89)80285-6","volume":"246","author":"BG Karlsson","year":"1989","unstructured":"Karlsson BG, Pascher T, Nordling M, Arvidsson RHA, Lundberg LG (1989) Expression of the blue copper protein azurin from Pseudomonas aeruginosa in Escherichia coli. FEBS Lett 246:211\u2013217. \nhttps:\/\/doi.org\/10.1016\/0014-5793(89)80285-6","journal-title":"FEBS Lett"},{"key":"9573_CR17","doi-asserted-by":"publisher","first-page":"196","DOI":"10.1016\/0014-5793(90)80669-A","volume":"261","author":"H Arai","year":"1990","unstructured":"Arai H, Sanbongi Y, Igarashi Y, Kodama T (1990) Cloning and sequencing of the gene encoding cytochrome c-551 from Pseudomonas aeruginosa. FEBS Lett 261:196\u2013198. \nhttps:\/\/doi.org\/10.1016\/0014-5793(90)80669-A","journal-title":"FEBS Lett"},{"key":"9573_CR18","doi-asserted-by":"publisher","first-page":"233","DOI":"10.1016\/S0165-022X(03)00049-6","volume":"55","author":"D Busso","year":"2003","unstructured":"Busso D, Kim R, Kim SH (2003) Expression of soluble recombinant proteins in a cell-free system using a 96-well format. J Biochem Biophys Methods 55:233\u2013240. \nhttps:\/\/doi.org\/10.1016\/S0165-022X(03)00049-6","journal-title":"J Biochem Biophys Methods"},{"key":"9573_CR19","doi-asserted-by":"publisher","first-page":"283","DOI":"10.1016\/0005-2728(90)90206-J","volume":"1019","author":"M van de Kamp","year":"1990","unstructured":"van de Kamp M, Hali FC, Rosato N, Agro AF, Canters GW (1990) Purification and characterization of a non-reconstitutable azurin, obtained by heterologous expression of the Pseudomonas aeruginosa azu gene in Escherichia coli. Biochim Biophys Acta Bioenerg 1019:283\u2013292. \nhttps:\/\/doi.org\/10.1016\/0005-2728(90)90206-J","journal-title":"Biochim Biophys Acta Bioenerg"},{"key":"9573_CR20","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0069023","volume":"8","author":"N Bernardes","year":"2013","unstructured":"Bernardes N, Ribeiro AS, Abreu S, Mota B, Matos RG, Arraiano CM, Seruca R, Paredes J, Fialho AM (2013) The bacterial protein azurin impairs invasion and FAK\/Src signaling in p-cadherin-overexpressing breast cancer cell models. PLoS One 8:e69023. \nhttps:\/\/doi.org\/10.1371\/journal.pone.0069023","journal-title":"PLoS One"},{"key":"9573_CR21","doi-asserted-by":"publisher","DOI":"10.3791\/2693","volume":"49","author":"AM Edwards","year":"2011","unstructured":"Edwards AM, Massey RC (2011) Invasion of human cells by a bacterial pathogen. J Vis Exp 49:e2693. \nhttps:\/\/doi.org\/10.3791\/2693","journal-title":"J Vis Exp"},{"key":"9573_CR22","doi-asserted-by":"publisher","DOI":"10.3791\/51759","volume":"90","author":"J Wu","year":"2014","unstructured":"Wu J, Pugh R, Laughlin RC, Andrews-Polymenis H, McClelland M, B\u00e4umler AJ, Adams LG (2014) High-throughput assay to phenotype Salmonella enterica Typhimurium association, invasion, and replication in macrophages. J Vis Exp 90:e51759. \nhttps:\/\/doi.org\/10.3791\/51759","journal-title":"J Vis Exp"},{"key":"9573_CR23","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0093549","volume":"9","author":"A Dostal","year":"2014","unstructured":"Dostal A, Gagnon M, Chassard C, Zimmermann MB, O\u2019Mahony L, Lacroix C (2014) Salmonella adhesion, invasion and cellular immune responses are differentially affected by iron concentrations in a combined in vitro gut fermentation-cell model. PLoS One 9:e93549. \nhttps:\/\/doi.org\/10.1371\/journal.pone.0093549","journal-title":"PLoS One"},{"key":"9573_CR24","doi-asserted-by":"publisher","first-page":"154","DOI":"10.1046\/j.1365-2672.2001.01371.x","volume":"91","author":"K Todoriki","year":"2001","unstructured":"Todoriki K, Mukai T, Sato S, Toba T (2001) Inhibition of adhesion of food-borne pathogens to caco-2 cells by Lactobacillus strains. J Appl Microbiol 91:154\u2013159. \nhttps:\/\/doi.org\/10.1046\/j.1365-2672.2001.01371.x","journal-title":"J Appl Microbiol"},{"key":"9573_CR25","doi-asserted-by":"publisher","first-page":"2853","DOI":"10.1099\/00221287-143-9-2853","volume":"143","author":"E Vijgenboom","year":"1997","unstructured":"Vijgenboom E, Busch JE, Canters GW (1997) In vivo studies disprove an obligatory role of azurin in denitrification in Pseudomonas aeruginosa and show that azu expression is under control of RpoS and ANR. Microbiology 143:2853\u20132863. \nhttps:\/\/doi.org\/10.1099\/00221287-143-9-2853","journal-title":"Microbiology"},{"key":"9573_CR26","doi-asserted-by":"publisher","first-page":"592","DOI":"10.1007\/s00284-012-0115-6","volume":"64","author":"B S\u00e1nchez","year":"2012","unstructured":"S\u00e1nchez B, Urdaci MC (2012) Extracellular proteins from Lactobacillus plantarum BMCM12 prevent adhesion of enteropathogens to mucin. Curr Microbiol 64:592\u2013596. \nhttps:\/\/doi.org\/10.1007\/s00284-012-0115-6","journal-title":"Curr Microbiol"},{"key":"9573_CR27","doi-asserted-by":"publisher","first-page":"17162","DOI":"10.1073\/pnas.0406038101","volume":"101","author":"MD Winfield","year":"2004","unstructured":"Winfield MD, Groisman EA (2004) Phenotypic differences between Salmonella and Escherichia coli resulting from the disparate regulation of homologous genes. Proc Natl Acad Sci U S A 101:17162\u201317167. \nhttps:\/\/doi.org\/10.1073\/pnas.0406038101","journal-title":"Proc Natl Acad Sci U S A"},{"key":"9573_CR28","doi-asserted-by":"publisher","first-page":"62","DOI":"10.1186\/2191-0855-3-62","volume":"3","author":"A Ganner","year":"2013","unstructured":"Ganner A, Stoiber C, Uhlik JT et al (2013) Quantitative evaluation of E. coli F4 and Salmonella Typhimurium binding capacity of yeast derivatives. AMB Express 3:62. \nhttps:\/\/doi.org\/10.1186\/2191-0855-3-62","journal-title":"AMB Express"},{"key":"9573_CR29","doi-asserted-by":"publisher","first-page":"319","DOI":"10.1016\/S0945-053X(00)00076-7","volume":"19","author":"J Heino","year":"2000","unstructured":"Heino J (2000) The collagen receptor integrins have distinct ligand recognition and signaling functions. Matrix Biol 19:319\u2013323. \nhttps:\/\/doi.org\/10.1016\/S0945-053X(00)00076-7","journal-title":"Matrix Biol"},{"key":"9573_CR30","doi-asserted-by":"publisher","first-page":"1122","DOI":"10.1111\/j.1574-6976.2012.00340.x","volume":"36","author":"B Singh","year":"2012","unstructured":"Singh B, Fleury C, Jalalvand F, Riesbeck K (2012) Human pathogens utilize host extracellular matrix proteins laminin and collagen for adhesion and invasion of the host. FEMS Microbiol Rev 36:1122\u20131180. \nhttps:\/\/doi.org\/10.1111\/j.1574-6976.2012.00340.x","journal-title":"FEMS Microbiol Rev"},{"key":"9573_CR31","doi-asserted-by":"publisher","first-page":"537","DOI":"10.1158\/0008-5472.CAN-08-2932","volume":"69","author":"BN Taylor","year":"2009","unstructured":"Taylor BN, Mehta RR, Yamada T, Lekmine F, Christov K, Chakrabarty AM, Green A, Bratescu L, Shilkaitis A, Beattie CW, Das Gupta TK (2009) Noncationic peptides obtained from azurin preferentially enter cancer cells. Cancer Res 69:537\u2013546. \nhttps:\/\/doi.org\/10.1158\/0008-5472.CAN-08-2932","journal-title":"Cancer Res"},{"key":"9573_CR32","doi-asserted-by":"publisher","first-page":"4223","DOI":"10.1074\/jbc.M110709200","volume":"277","author":"GA Di Lullo","year":"2002","unstructured":"Di Lullo GA, Sweeney SM, Korkko J et al (2002) Mapping the ligand-binding sites and disease-associated mutations on the most abundant protein in the human, type I collagen. J Biol Chem 277:4223\u20134231. \nhttps:\/\/doi.org\/10.1074\/jbc.M110709200","journal-title":"J Biol Chem"}],"container-title":["Probiotics and Antimicrobial Proteins"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s12602-019-09573-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s12602-019-09573-2\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s12602-019-09573-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,7,28]],"date-time":"2020-07-28T23:47:12Z","timestamp":1595980032000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s12602-019-09573-2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,7,30]]},"references-count":32,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2020,6]]}},"alternative-id":["9573"],"URL":"https:\/\/doi.org\/10.1007\/s12602-019-09573-2","relation":{},"ISSN":["1867-1306","1867-1314"],"issn-type":[{"value":"1867-1306","type":"print"},{"value":"1867-1314","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,7,30]]},"assertion":[{"value":"30 July 2019","order":1,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Compliance with Ethical Standards"}},{"value":"The authors declare that they have no conflict of interest.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of Interest"}},{"value":"This study does not contain any studies with human or animal intervention.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical Approval"}}]}}