{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T18:10:51Z","timestamp":1792519851169,"version":"4.5.0"},"reference-count":30,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2020,6,24]],"date-time":"2020-06-24T00:00:00Z","timestamp":1592956800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2020,6,24]],"date-time":"2020-06-24T00:00:00Z","timestamp":1592956800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Probiotics & Antimicro. Prot."],"published-print":{"date-parts":[[2021,2]]},"DOI":"10.1007\/s12602-020-09675-2","type":"journal-article","created":{"date-parts":[[2020,6,24]],"date-time":"2020-06-24T11:02:48Z","timestamp":1592996568000},"page":"163-172","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Novel Potential Probiotic Lactobacilli for Prevention and Treatment of Vulvovaginal Infections"],"prefix":"10.1007","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4822-5597","authenticated-orcid":false,"given":"Monika","family":"Kumherov\u00e1","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristina","family":"Vesel\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michaela","family":"Kosov\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jarom\u00edr","family":"Ma\u0161ata","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u0160\u00e1rka","family":"Hor\u00e1\u010dkov\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"\u0160midrkal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,6,24]]},"reference":[{"key":"9675_CR1","first-page":"3","volume-title":"Biological safety: principles and practices","author":"P Granato","year":"2006","unstructured":"Granato P (2006) Microbial flora of humans and microbial virulence factors. In: Wooley D, Byers K (eds) Biological safety: principles and practices. ASM Press, Washington, pp 3\u201317"},{"key":"9675_CR2","doi-asserted-by":"publisher","first-page":"479","DOI":"10.1007\/s00404-013-3064-9","volume":"289","author":"S Borges","year":"2014","unstructured":"Borges S, Silva J, Teixeira P (2014) The role of lactobacilli and probiotics in maintaining vaginal health. Arch Gynecol Obstet 289:479\u2013489. https:\/\/doi.org\/10.1007\/s00404-013-3064-9","journal-title":"Arch Gynecol Obstet"},{"key":"9675_CR3","doi-asserted-by":"publisher","first-page":"543","DOI":"10.1016\/S1286-4579(00)00313-0","volume":"2","author":"S Boris","year":"2000","unstructured":"Boris S, Barb\u00e9s C (2000) Role played by lactobacilli in controlling the population of vaginal pathogens. Microbes Infect 2:543\u2013546. https:\/\/doi.org\/10.1016\/S1286-4579(00)00313-0","journal-title":"Microbes Infect"},{"key":"9675_CR4","doi-asserted-by":"publisher","first-page":"11060","DOI":"10.1073\/pnas.1502875112","volume":"112","author":"DB DiGiulio","year":"2015","unstructured":"DiGiulio DB, Callahan BJ, McMurdie PJ, Costello EK, Lyell DJ, Robaczewska A, Sun CL, Goltsman DSA, Wong RJ, Shaw G, Stevenson DK, Holmes SP, Relman DA (2015) Temporal and spatial variation of the human microbiota during pregnancy. Proc Natl Acad Sci U S A 112:11060\u201311065. https:\/\/doi.org\/10.1073\/pnas.1502875112","journal-title":"Proc Natl Acad Sci U S A"},{"key":"9675_CR5","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1371\/journal.pone.0098514","volume":"9","author":"MRS Walther-Ant\u00f3nio","year":"2014","unstructured":"Walther-Ant\u00f3nio MRS, Jeraldo P, Berg Miller ME, Yeoman CJ, Nelson KE, Wilson BA, White BA, Chia N, Creedon DJ (2014) Pregnancy\u2019s stronghold on the vaginal microbiome. PLoS One 9:1\u201310. https:\/\/doi.org\/10.1371\/journal.pone.0098514","journal-title":"PLoS One"},{"key":"9675_CR6","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/srep05136","volume":"4","author":"L Petricevic","year":"2014","unstructured":"Petricevic L, Domig KJ, Nierscher FJ, Sandhofer MJ, Fidesser M, Krondorfer I, Husslein P, Kneifel W, Kiss H (2014) Characterisation of the vaginal Lactobacillus microbiota associated with preterm delivery. Sci Rep 4:1\u20136. https:\/\/doi.org\/10.1038\/srep05136","journal-title":"Sci Rep"},{"key":"9675_CR7","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1371\/journal.pone.0144181","volume":"10","author":"A Farr","year":"2015","unstructured":"Farr A, Kiss H, Hagmann M, Machal S, Holzer I, Kueronya V, Husslein PW, Petricevic L (2015) Role of Lactobacillus species in the intermediate vaginal flora in early pregnancy: a retrospective cohort study. PLoS One 10:1\u201312. https:\/\/doi.org\/10.1371\/journal.pone.0144181","journal-title":"PLoS One"},{"key":"9675_CR8","first-page":"349","volume":"57","author":"JS Park","year":"2005","unstructured":"Park JS, Park CW, Lockwood CJ, Norwitz ER (2005) Role of cytokines in preterm labor and birth. Minerva Ginecol 57:349\u2013366","journal-title":"Minerva Ginecol"},{"key":"9675_CR9","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3389\/fmicb.2016.01031","volume":"7","author":"M Nuriel-Ohayon","year":"2016","unstructured":"Nuriel-Ohayon M, Neuman H, Koren O (2016) Microbial changes during pregnancy, birth, and infancy. Front Microbiol 7:1\u201313. https:\/\/doi.org\/10.3389\/fmicb.2016.01031","journal-title":"Front Microbiol"},{"key":"9675_CR10","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1126\/sciimmunol.aah4576","volume":"1","author":"E Boldenow","year":"2016","unstructured":"Boldenow E, Gendrin C, Ngo L, Bierle C, Vornhagen J, Coleman M, Merillat S, Armistead B, Whidbey C, Alishetti V, Santana-Ufret V, Ogle J, Gough M, Srinouanprachanh S, MacDonald JW, Bammler TK, Bansal A, Liggitt HD, Rajagopal L, Adams Waldorf KM (2016) Group B Streptococcus circumvents neutrophils and neutrophil extracellular traps during amniotic cavity invasion and preterm labor. Sci Immunol 1:1\u201313. https:\/\/doi.org\/10.1126\/sciimmunol.aah4576","journal-title":"Sci Immunol"},{"key":"9675_CR11","doi-asserted-by":"publisher","first-page":"677","DOI":"10.1007\/s10482-011-9666-y","volume":"101","author":"S Borges","year":"2012","unstructured":"Borges S, Silva J, Teixeira P (2012) Survival and biofilm formation by group B streptococci in simulated vaginal fluid at different pHs. Antonie Leeuwenhoek 101:677\u2013682. https:\/\/doi.org\/10.1007\/s10482-011-9666-y","journal-title":"Antonie Leeuwenhoek"},{"key":"9675_CR12","doi-asserted-by":"publisher","first-page":"1737","DOI":"10.1056\/NEJM199512283332604","volume":"333","author":"SL Hillier","year":"1995","unstructured":"Hillier SL, Nugent RP, Eschenbach DA, Krohn MA, Gibbs RS, Martin DH, Frances Coach M, Edelman R, Pastorek JG, Vijaya Rao A, McNellis D, Regan JA, Carey C, Klebanoff MA (1995) Association between bacterial vaginosis and preterm delivery of a low-birth-weight infant. N Engl J Med 333:1737\u20131742. https:\/\/doi.org\/10.1056\/NEJM199512283332604","journal-title":"N Engl J Med"},{"key":"9675_CR13","doi-asserted-by":"publisher","first-page":"741","DOI":"10.1016\/B978-0-12-802189-7.00056-3","volume-title":"Probiotics, prebiotics, and synbiotics - bioactive foods in health promotion","author":"S Borges","year":"2016","unstructured":"Borges S, Barbosa J, Teixeira P (2016) Gynecological health and probiotics. In: Watson RR, Preedy VR (eds) Probiotics, prebiotics, and synbiotics - bioactive foods in health promotion. Elsevier, London, pp 741\u2013752"},{"key":"9675_CR14","doi-asserted-by":"publisher","first-page":"1985","DOI":"10.1128\/IAI.66.5.1985-1989.1998","volume":"66","author":"S Boris","year":"1998","unstructured":"Boris S, Su\u00e1rez JE, V\u00e1zquez F, Barb\u00e9s C (1998) Adherence of human vaginal lactobacilli to vaginal epithelial cells and interaction with uropathogens. Infect Immun 66:1985\u20131989","journal-title":"Infect Immun"},{"key":"9675_CR15","doi-asserted-by":"publisher","first-page":"1297","DOI":"10.1111\/jam.12459","volume":"116","author":"MC Verdenelli","year":"2014","unstructured":"Verdenelli MC, Coman MM, Cecchini C, Silvi S, Orpianesi C, Cresci A (2014) Evaluation of antipathogenic activity and adherence properties of human Lactobacillus strains for vaginal formulations. J Appl Microbiol 116:1297\u20131307. https:\/\/doi.org\/10.1111\/jam.12459","journal-title":"J Appl Microbiol"},{"key":"9675_CR16","doi-asserted-by":"publisher","first-page":"437","DOI":"10.1093\/ajcn\/73.2.437s","volume":"78","author":"G Reid","year":"2001","unstructured":"Reid G (2001) Probiotic agents to protect the urogenital tract against infection. Am J Clin Nutr 78:437\u2013443. https:\/\/doi.org\/10.1093\/ajcn\/73.2.437s","journal-title":"Am J Clin Nutr"},{"key":"9675_CR17","first-page":"351","volume-title":"Applications of polymers in drug delivery","author":"MS Lalan","year":"2011","unstructured":"Lalan MS, Misra A (2011) Applications of polymers in vaginal drug delivery. In: Misra A, Shahiwala A (eds) Applications of polymers in drug delivery. Smithers Rapra, Shawbury, pp 351\u2013377"},{"key":"9675_CR18","doi-asserted-by":"publisher","first-page":"3300","DOI":"10.5897\/AJMR11.481","volume":"5","author":"M Amin","year":"2011","unstructured":"Amin M, Goodarzi H, Orang Z, Farsi S, Jorfi M (2011) Isolation and identification of Lactobacillus species from the vagina and their antimicrobial properties. Afr J Microbiol Res 5:3300\u20133304. https:\/\/doi.org\/10.5897\/AJMR11.481","journal-title":"Afr J Microbiol Res"},{"key":"9675_CR19","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/2049-2618-2-4","volume":"4","author":"R Romero","year":"2014","unstructured":"Romero R, Hassan SS, Gajer P, Tarca AL, Fadrosh DW, Nikita L, Galuppi M, Lamont RL, Chaemsaithong P, Miranda J, Chaiworapongsa T, Ravel J (2014) The composition and stability of the vaginal microbiota of normal pregnant women is different from that of non-pregnant women. Microbiome 4:1\u201319. https:\/\/doi.org\/10.1186\/2049-2618-2-4","journal-title":"Microbiome"},{"key":"9675_CR20","doi-asserted-by":"publisher","first-page":"1402","DOI":"10.1111\/j.1471-0528.2007.01412.x","volume":"114","author":"H Kiss","year":"2007","unstructured":"Kiss H, K\u00f6gler B, Petricevic L, Sauerzapf I, Klayraung S, Domig K, Viernstein H, Kneifel W (2007) Vaginal Lactobacillus microbiota of healthy women in the late first trimester of pregnancy. BJOG 114:1402\u20131407. https:\/\/doi.org\/10.1111\/j.1471-0528.2007.01412.x","journal-title":"BJOG"},{"key":"9675_CR21","doi-asserted-by":"publisher","first-page":"120","DOI":"10.1099\/00222615-45-2-120","volume":"45","author":"IJ Rosenstein","year":"1996","unstructured":"Rosenstein IJ, Morgan DJ, Sheehan M, Lamont RF, Taylor-Robinson D (1996) Bacterial vaginosis in pregnancy: distribution of bacterial species in different gram-stain categories of the vaginal flora. J Med Microbiol 45:120\u2013126. https:\/\/doi.org\/10.1099\/00222615-45-2-120","journal-title":"J Med Microbiol"},{"key":"9675_CR22","doi-asserted-by":"publisher","first-page":"845","DOI":"10.1016\/j.resmic.2017.04.004","volume":"168","author":"GGG Donders","year":"2017","unstructured":"Donders GGG, Bellen G, Grinceviciene S, Ruban K, Vieira-Baptista P (2017) Aerobic vaginitis: no longer a stranger. Res Microbiol 168:845\u2013858. https:\/\/doi.org\/10.1016\/j.resmic.2017.04.004","journal-title":"Res Microbiol"},{"key":"9675_CR23","doi-asserted-by":"publisher","first-page":"69","DOI":"10.1080\/10647440400028136","volume":"13","author":"M Strus","year":"2005","unstructured":"Strus M, Kucharska A, Kukla G, Brzychczy-Wloch M, Maresz K, Heczko PB (2005) The in vitro activity of vaginal Lactobacillus with probiotic properties against Candida. Infect Dis Obstet Gynecol 13:69\u201375. https:\/\/doi.org\/10.1080\/10647440400028136","journal-title":"Infect Dis Obstet Gynecol"},{"key":"9675_CR24","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3402\/mehd.v27.30484","volume":"27","author":"P H\u00fctt","year":"2016","unstructured":"H\u00fctt P, Lapp E, \u0160t\u0161epetova J, Smidt I, Taelma H, Borovkova N, Oopkaup H, Ahelik A, R\u00f6\u00f6p T, Hoidmets D, Samuel K, Salumets A, M\u00e4ndar R (2016) Characterisation of probiotic properties in human vaginal lactobacilli strains. Microb Ecol Health Dis 27:1\u20139. https:\/\/doi.org\/10.3402\/mehd.v27.30484","journal-title":"Microb Ecol Health Dis"},{"key":"9675_CR25","doi-asserted-by":"publisher","first-page":"192","DOI":"10.15171\/ijb.1432","volume":"14","author":"H Bouridane","year":"2016","unstructured":"Bouridane H, Sifour M, Idoui T, Annick L, Thonard P (2016) Technological and probiotic traits of the lactobacilli isolated from vaginal tract of the healthy women for probiotic use. Iran J Biotech 14:192\u2013201. https:\/\/doi.org\/10.15171\/ijb.1432","journal-title":"Iran J Biotech"},{"key":"9675_CR26","doi-asserted-by":"publisher","first-page":"3260","DOI":"10.1128\/JCM.41.7.3260-3264.2003","volume":"41","author":"LK Rabe","year":"2003","unstructured":"Rabe LK, Hillier SL (2003) Optimization of media for detection of hydrogen peroxide production by Lactobacillus species. J Clin Microbiol 41:3260\u20133264. https:\/\/doi.org\/10.1128\/JCM.41.7.3260-3264.2003","journal-title":"J Clin Microbiol"},{"key":"9675_CR27","doi-asserted-by":"publisher","first-page":"6","DOI":"10.1016\/j.anaerobe.2013.04.009","volume":"22","author":"S Kaewnopparat","year":"2013","unstructured":"Kaewnopparat S, Dangmanee N, Kaewnopparat N, Srichana T, Chulasiri M, Settharaksa S (2013) In vitro probiotic properties of Lactobacillus fermentum SK5 isolated from vagina of a healthy woman. Anaerobe 22:6\u201313. https:\/\/doi.org\/10.1016\/j.anaerobe.2013.04.009","journal-title":"Anaerobe"},{"key":"9675_CR28","doi-asserted-by":"publisher","first-page":"48","DOI":"10.1080\/08910600701837964","volume":"20","author":"KC Anukam","year":"2008","unstructured":"Anukam KC, Reid G (2008) Effects of metronidazole on growth of Gardnerella vaginalis ATCC 14018, probiotic Lactobacillus rhamnosus GR-1 and vaginal isolate Lactobacillus plantarum KCA. Microb Ecol Health Dis 20:48\u201352. https:\/\/doi.org\/10.1080\/08910600701837964","journal-title":"Microb Ecol Health Dis"},{"key":"9675_CR29","doi-asserted-by":"publisher","first-page":"4576","DOI":"10.1128\/AEM.00926-13","volume":"79","author":"S Malik","year":"2013","unstructured":"Malik S, Petrova MI, Claes IJJ, Verhoeven TLA, Busschaert P, Vaneechoutte M, Lievens B, Lambrichts I, Siezen RJ, Balzarini J, Vanderleyden J, Lebeer S (2013) The highly autoaggregative and adhesive phenotype of the vaginal Lactobacillus plantarum strain CMPG5300 is sortase dependent. J Appl Environ Microbiol 79:4576\u20134585. https:\/\/doi.org\/10.1128\/AEM.00926-13","journal-title":"J Appl Environ Microbiol"},{"key":"9675_CR30","doi-asserted-by":"publisher","first-page":"981","DOI":"10.1046\/j.1365-2672.2003.01915.x","volume":"94","author":"B Kos","year":"2003","unstructured":"Kos B, \u0160u\u0161kovic J, Vukovic S, \u0160impraga M, Frece J, Mato\u0161ic S (2003) Adhesion and aggregation ability of probiotic strain Lactobacillus acidophilus M92. J Appl Microbiol 94:981\u2013987. https:\/\/doi.org\/10.1046\/j.1365-2672.2003.01915.x","journal-title":"J Appl Microbiol"}],"container-title":["Probiotics and Antimicrobial Proteins"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s12602-020-09675-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s12602-020-09675-2\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s12602-020-09675-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,6,23]],"date-time":"2021-06-23T23:08:29Z","timestamp":1624489709000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s12602-020-09675-2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,6,24]]},"references-count":30,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2021,2]]}},"alternative-id":["9675"],"URL":"https:\/\/doi.org\/10.1007\/s12602-020-09675-2","relation":{},"ISSN":["1867-1306","1867-1314"],"issn-type":[{"value":"1867-1306","type":"print"},{"value":"1867-1314","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,6,24]]},"assertion":[{"value":"24 June 2020","order":1,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and\/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. Sample collecting was approved by the Ethics Committee of the General University Hospital, Prague.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Compliance with Ethical Standards"}},{"value":"The authors declare that they have no conflict of interest.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of Interest"}},{"value":"Informed consent was obtained from all individual participants included in the study.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Informed Consent"}}]}}