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Prot."],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The urgent need for new antimicrobials is driving the optimization of proline-rich antimicrobial peptides (PrAMPs) as a basis for novel antibiotics to combat multidrug-resistant pathogens. The PrAMP B7-005 has emerged from this process, displaying a broader spectrum of activity compared to similar native PrAMPs and reduced reliance on the bacterial transporter SbmA for its action. While the compatibility and interactions of B7-005 with various mammalian cell types have been investigated, most information on its molecular mechanism of antibacterial action has so far been limited to\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    . In this study, we investigated the antimicrobial potency and mechanisms of action of B7-005 across the full panel of ESKAPE pathogens, with\n                    <jats:italic>E. coli<\/jats:italic>\n                    included for comparison (ESKAPE\u2009+\u2009E). The potential of B7-005 to eradicate these pathogens was evaluated in both planktonic and biofilm forms, revealing distinct bactericidal and anti-biofilm effects across the ESKAPE\u2009+\u2009E pathogens. B7-005\u2019s mechanism of action also varied depending on the target microorganism, ranging from intracellular inhibition of protein synthesis without membrane damage to varying levels of membrane permeabilization. Notably, B7-005 consistently inhibited protein synthesis across all ESKAPE\u2009+\u2009E pathogens, suggesting a possible combination of lytic and non-lytic mechanisms. Furthermore, biochemical analysis of its inhibitory effect on protein synthesis demonstrated that, despite acquiring membrane-destabilizing properties, B7-005 still blocks ribosome progression into the elongation phase, consistent with Class I PrAMPs. B7-005 thus retains the essential characteristics of native PrAMPs while offering a broadened spectrum of activity, highlighting its potential as a lead compound in the development of new antibiotics.\n                  <\/jats:p>","DOI":"10.1007\/s12602-025-10568-5","type":"journal-article","created":{"date-parts":[[2025,6,5]],"date-time":"2025-06-05T05:32:54Z","timestamp":1749101574000},"page":"1170-1186","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Mechanistic Divergence and Differential Antibacterial Potency of the Proline-Rich Antimicrobial Peptide B7-005 Across ESKAPE +\u2009E Pathogens"],"prefix":"10.1007","volume":"18","author":[{"given":"Adriana","family":"Di Stasi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Capolla","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martino","family":"Morici","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Bozzer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Max","family":"Berger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabrina","family":"Pacor","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thuy Duong","family":"Pham","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberto","family":"Spurio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Attilio","family":"Fabbretti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paolo","family":"Macor","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel N.","family":"Wilson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco","family":"Scocchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mario","family":"Mardirossian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,6,5]]},"reference":[{"key":"10568_CR1","doi-asserted-by":"publisher","first-page":"e00181","DOI":"10.1128\/CMR.00181-19","volume":"33","author":"DMP De Oliveira","year":"2020","unstructured":"De Oliveira DMP, Forde BM, Kidd TJ, Harris PNA, Schembri MA, Beatson SA, Paterson DL, Walker MJ (2020) Antimicrobial resistance in ESKAPE pathogens. 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