{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T11:50:41Z","timestamp":1780573841791,"version":"3.54.1"},"reference-count":38,"publisher":"Springer Science and Business Media LLC","issue":"4","license":[{"start":{"date-parts":[[2021,2,22]],"date-time":"2021-02-22T00:00:00Z","timestamp":1613952000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2021,2,22]],"date-time":"2021-02-22T00:00:00Z","timestamp":1613952000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"}],"funder":[{"name":"National Major Research and Development Program of China","award":["2017YFC1200902"],"award-info":[{"award-number":["2017YFC1200902"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Neurotox Res"],"published-print":{"date-parts":[[2021,8]]},"DOI":"10.1007\/s12640-021-00337-x","type":"journal-article","created":{"date-parts":[[2021,2,22]],"date-time":"2021-02-22T07:02:51Z","timestamp":1613977371000},"page":"1044-1053","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Recombinant L-HN Fusion Antigen Derived from the L and HN Domains of Botulinum Neurotoxin B Stimulates a Protective Antibody Response Against Active Neurotoxin"],"prefix":"10.1007","volume":"39","author":[{"given":"Zhen","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian-Sheng","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shan","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rong","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5124-9688","authenticated-orcid":false,"given":"Yun-Zhou","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhi-Xin","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,2,22]]},"reference":[{"key":"337_CR1","doi-asserted-by":"publisher","first-page":"15776","DOI":"10.1038\/srep15776","volume":"5","author":"BV Ayyar","year":"2015","unstructured":"Ayyar BV, Tajhya RB, Beeton C, Atassi MZ (2015) Antigenic sites on the HN domain of botulinum neurotoxin A stimulate protective antibody responses against active toxin. Sci Rep 5:15776. https:\/\/doi.org\/10.1038\/srep15776","journal-title":"Sci Rep"},{"key":"337_CR2","doi-asserted-by":"publisher","first-page":"1314","DOI":"10.1128\/IAI.01025-07","volume":"76","author":"MR Baldwin","year":"2008","unstructured":"Baldwin MR, Tepp WH, Przedpelski A, Pier CL, Bradshaw M, Johnson EA, Barbieri JT (2008) Subunit vaccine against the seven serotypes of botulism. Infect Immun 76:1314\u20131318. https:\/\/doi.org\/10.1128\/IAI.01025-07","journal-title":"Infect Immun"},{"issue":"2","key":"337_CR3","doi-asserted-by":"publisher","first-page":"183","DOI":"10.1093\/infdis\/jit449","volume":"209","author":"JR Barash","year":"2014","unstructured":"Barash JR, Arnon SS (2014) A novel strain of Clostridium botulinum that produces type B and type H botulinum toxins. J Infect Dis 209(2):183\u2013191. https:\/\/doi.org\/10.1093\/infdis\/jit449","journal-title":"J Infect Dis"},{"key":"337_CR4","doi-asserted-by":"publisher","first-page":"7","DOI":"10.1186\/s40696-016-0017-4","volume":"2","author":"T Berger","year":"2016","unstructured":"Berger T, Eisenkraft A, Bar-Haim E, Kassirer M, Aran AA, Fogel I (2016) Toxins as biological weapons for terror-characteristics, challenges and medical countermeasures: a mini-review. Disaster Mil Med 2:7. https:\/\/doi.org\/10.1186\/s40696-016-0017-4","journal-title":"Disaster Mil Med"},{"key":"337_CR5","doi-asserted-by":"publisher","first-page":"e43845","DOI":"10.1371\/journal.pone.0043845","volume":"7","author":"C Chen","year":"2012","unstructured":"Chen C, Wang S, Wang H, Mao X, Zhang T, Ji G, Shi X, Xia T, Lu W, Zhang D, Dai J, Guo Y (2012) Potent neutralization of botulinum neurotoxin\/B by synergistic action of antibodies recognizing protein and ganglioside receptor binding domain. PLoS ONE 7:e43845. https:\/\/doi.org\/10.1371\/journal.pone.0043845","journal-title":"PLoS ONE"},{"key":"337_CR6","doi-asserted-by":"publisher","first-page":"5068","DOI":"10.3390\/toxins7124863","volume":"7","author":"LW Cheng","year":"2015","unstructured":"Cheng LW, Henderson TD 2nd, Lam TI, Stanker LH (2015) Use of monoclonal antibodies in the sensitive detection and neutralization of botulinum neurotoxin serotype B. Toxins 7:5068\u20135078. https:\/\/doi.org\/10.3390\/toxins7124863","journal-title":"Toxins"},{"key":"337_CR7","doi-asserted-by":"publisher","unstructured":"Davies JR, Liu SM, Acharya KR\u00a0(2018) Variations in the Botulinum Neurotoxin Binding Domain and the Potential for Novel Therapeutics Toxins 10 https:\/\/doi.org\/10.3390\/toxins10100421","DOI":"10.3390\/toxins10100421"},{"key":"337_CR8","doi-asserted-by":"publisher","first-page":"811","DOI":"10.1146\/annurev-biochem-013118-111654","volume":"88","author":"M Dong","year":"2019","unstructured":"Dong M, Masuyer G, Stenmark P (2019) Botulinum and tetanus neurotoxins. Annu Rev Biochem 88:811\u2013837. https:\/\/doi.org\/10.1146\/annurev-biochem-013118-111654","journal-title":"Annu Rev Biochem"},{"key":"337_CR9","doi-asserted-by":"publisher","first-page":"3405","DOI":"10.3390\/toxins7093405","volume":"7","author":"Y Fan","year":"2015","unstructured":"Fan Y, Dong J, Lou J, Wen W, Conrad F, Geren IN, Garcia-Rodriguez C, Smith TJ, Smith LA, Ho M, Pires-Alves M, Wilson BA, Marks JD (2015) Monoclonal antibodies that inhibit the proteolytic activity of botulinum neurotoxin serotype\/B. Toxins 7:3405\u20133423. https:\/\/doi.org\/10.3390\/toxins7093405","journal-title":"Toxins"},{"key":"337_CR10","doi-asserted-by":"publisher","first-page":"587","DOI":"10.1016\/j.toxicon.2009.01.037","volume":"54","author":"KA Foster","year":"2009","unstructured":"Foster KA (2009) Engineered toxins: new therapeutics. Toxicon 54:587\u2013592. https:\/\/doi.org\/10.1016\/j.toxicon.2009.01.037","journal-title":"Toxicon"},{"key":"337_CR11","doi-asserted-by":"publisher","first-page":"321","DOI":"10.1093\/protein\/gzq111","volume":"24","author":"C Garcia-Rodriguez","year":"2011","unstructured":"Garcia-Rodriguez C, Geren IN, Lou J, Conrad F, Forsyth C, Wen W, Chakraborti S, Zao H, Manzanarez G, Smith TJ, Brown J, Tepp WH, Liu N, Wijesuriya S, Tomic MT, Johnson EA, Smith LA, Marks JD (2011) Neutralizing human monoclonal antibodies binding multiple serotypes of botulinum neurotoxin. Protein Eng Des Sel 24:321\u2013331. https:\/\/doi.org\/10.1093\/protein\/gzq111","journal-title":"Protein Eng Des Sel"},{"key":"337_CR12","doi-asserted-by":"publisher","unstructured":"Janik E, Ceremuga M, Saluk-Bijak J, Bijak M (2019) Biological Toxins as the Potential Tools for Bioterrorism Int J Mol Sci 20 https:\/\/doi.org\/10.3390\/ijms20051181","DOI":"10.3390\/ijms20051181"},{"key":"337_CR13","doi-asserted-by":"publisher","first-page":"42","DOI":"10.1016\/j.jim.2008.05.009","volume":"337","author":"RG Jones","year":"2008","unstructured":"Jones RG, Liu Y, Rigsby P, Sesardic D (2008) An improved method for development of toxoid vaccines and antitoxins. J Immunol Methods 337:42\u201348. https:\/\/doi.org\/10.1016\/j.jim.2008.05.009","journal-title":"J Immunol Methods"},{"key":"337_CR14","doi-asserted-by":"publisher","first-page":"2041","DOI":"10.1016\/j.vaccine.2018.02.042","volume":"36","author":"JM Khouri","year":"2018","unstructured":"Khouri JM, Motter RN, Arnon SS (2018) Safety and immunogenicity of investigational recombinant botulinum vaccine, rBV A\/B, in volunteers with pre-existing botulinum toxoid immunity. Vaccine 36:2041\u20132048. https:\/\/doi.org\/10.1016\/j.vaccine.2018.02.042","journal-title":"Vaccine"},{"key":"337_CR15","doi-asserted-by":"publisher","first-page":"7678","DOI":"10.1038\/srep07678","volume":"5","author":"B Liu","year":"2015","unstructured":"Liu B, Shi D, Chang S, Gong X, Yu Y, Sun Z, Wu J (2015) Characterization and immunological activity of different forms of recombinant secreted Hc of botulinum neurotoxin serotype B products expressed in yeast. Sci Rep 5:7678. https:\/\/doi.org\/10.1038\/srep07678","journal-title":"Sci Rep"},{"key":"337_CR16","doi-asserted-by":"publisher","first-page":"2978","DOI":"10.1016\/j.vaccine.2020.02.060","volume":"38","author":"FJ Liu","year":"2020","unstructured":"Liu FJ, Shi DY, Mao YY, Xiong XH, Lu JS, Pang XB, Dong XJ, Yang ZX, Yu YZ (2020) Immunological characterisation and immunoprotective efficacy of functional domain antigens of botulinum neurotoxin serotype A. Vaccine 38:2978\u20132983. https:\/\/doi.org\/10.1016\/j.vaccine.2020.02.060","journal-title":"Vaccine"},{"key":"337_CR17","doi-asserted-by":"publisher","unstructured":"Lou J, Marks JD (2018) Botulinum Neurotoxins (BoNTs)-Antibody and Vaccine Toxins 10 https:\/\/doi.org\/10.3390\/toxins10120495","DOI":"10.3390\/toxins10120495"},{"key":"337_CR18","doi-asserted-by":"publisher","first-page":"52","DOI":"10.1016\/j.jsb.2010.11.010","volume":"174","author":"G Masuyer","year":"2011","unstructured":"Masuyer G, Beard M, Cadd VA, Chaddock JA, Acharya KR (2011) Structure and activity of a functional derivative of Clostridium botulinum neurotoxin B. J Struct Biol 174:52\u201357. https:\/\/doi.org\/10.1016\/j.jsb.2010.11.010","journal-title":"J Struct Biol"},{"key":"337_CR19","doi-asserted-by":"publisher","first-page":"1466","DOI":"10.1107\/S1744309111034671","volume":"67","author":"G Masuyer","year":"2011","unstructured":"Masuyer G, Stancombe P, Chaddock JA, Acharya KR (2011) Structures of engineered Clostridium botulinum neurotoxin derivatives. Acta Crystallogr Sect F Struct Biol Cryst Commun 67:1466\u20131472. https:\/\/doi.org\/10.1107\/S1744309111034671","journal-title":"Acta Crystallogr Sect F Struct Biol Cryst Commun"},{"key":"337_CR20","doi-asserted-by":"publisher","unstructured":"Matsumura T, Amatsu S, Misaki R, Yutani M, Du A, Kohda T, Fujiyama K, Ikuta K, Fujinaga Y (2020) Fully Human Monoclonal Antibodies Effectively Neutralizing Botulinum Neurotoxin Serotype B Toxins 12 https:\/\/doi.org\/10.3390\/toxins12050302","DOI":"10.3390\/toxins12050302"},{"key":"337_CR21","doi-asserted-by":"publisher","first-page":"141","DOI":"10.1016\/j.ab.2012.08.005","volume":"430","author":"Q Meng","year":"2012","unstructured":"Meng Q, Garcia-Rodriguez C, Manzanarez G, Silberg MA, Conrad F, Bettencourt J, Pan X, Breece T, To R, Li M, Lee D, Thorner L, Tomic MT, Marks JD (2012) Engineered domain-based assays to identify individual antibodies in oligoclonal combinations targeting the same protein. Anal Biochem 430:141\u2013150. https:\/\/doi.org\/10.1016\/j.ab.2012.08.005","journal-title":"Anal Biochem"},{"key":"337_CR22","doi-asserted-by":"publisher","first-page":"130","DOI":"10.1016\/j.anaerobe.2014.06.003","volume":"28","author":"GM Moreira","year":"2014","unstructured":"Moreira GM, Cunha CE, Salvarani FM, Goncalves LA, Pires PS, Conceicao FR, Lobato FC (2014) Production of recombinant botulism antigens: a review of expression systems. Anaerobe 28:130\u2013136. https:\/\/doi.org\/10.1016\/j.anaerobe.2014.06.003","journal-title":"Anaerobe"},{"key":"337_CR23","doi-asserted-by":"publisher","first-page":"200","DOI":"10.1124\/pr.116.012658","volume":"69","author":"M Pirazzini","year":"2017","unstructured":"Pirazzini M, Rossetto O, Eleopra R, Montecucco C (2017) Botulinum neurotoxins: biology, pharmacology, and toxicology. Pharmacol Rev 69:200\u2013235. https:\/\/doi.org\/10.1124\/pr.116.012658","journal-title":"Pharmacol Rev"},{"key":"337_CR24","doi-asserted-by":"publisher","first-page":"2107","DOI":"10.1016\/s0264-410x(02)00058-0","volume":"20","author":"PR Pittman","year":"2002","unstructured":"Pittman PR, Hack D, Mangiafico J, Gibbs P, McKee KT Jr, Friedlander AM, Sjogren MH (2002) Antibody response to a delayed booster dose of anthrax vaccine and botulinum toxoid. Vaccine 20:2107\u20132115. https:\/\/doi.org\/10.1016\/s0264-410x(02)00058-0","journal-title":"Vaccine"},{"key":"337_CR25","doi-asserted-by":"publisher","first-page":"1161","DOI":"10.1080\/19420862.2015.1082016","volume":"7","author":"C Rasetti-Escargueil","year":"2015","unstructured":"Rasetti-Escargueil C, Avril A, Chahboun S, Tierney R, Bak N, Miethe S, Mazuet C, Popoff MR, Thullier P, Hust M, Pelat T, Sesardic D (2015) Development of human-like scFv-Fc antibodies neutralizing Botulinum toxin serotype B. MAbs 7:1161\u20131177. https:\/\/doi.org\/10.1080\/19420862.2015.1082016","journal-title":"MAbs"},{"key":"337_CR26","doi-asserted-by":"publisher","unstructured":"Rasetti-Escargueil C, Popoff\u00a0 MR (2019) Antibodies and Vaccines against Botulinum Toxins: Available Measures and Novel Approaches Toxins 11 https:\/\/doi.org\/10.3390\/toxins11090528","DOI":"10.3390\/toxins11090528"},{"key":"337_CR27","doi-asserted-by":"publisher","unstructured":"Rossetto O, Montecucco C (2019) Tables of Toxicity of Botulinum and Tetanus Neurotoxins Toxins 11 https:\/\/doi.org\/10.3390\/toxins11120686","DOI":"10.3390\/toxins11120686"},{"key":"337_CR28","doi-asserted-by":"publisher","first-page":"155","DOI":"10.1016\/j.vaccine.2017.11.008","volume":"36","author":"B Sahay","year":"2018","unstructured":"Sahay B, Colliou N, Zadeh M, Ge Y, Gong M, Owen JL, Valletti M, Jobin C, Mohamadzadeh M (2018) Dual-route targeted vaccine protects efficiently against botulinum neurotoxin A complex. Vaccine 36:155\u2013164. https:\/\/doi.org\/10.1016\/j.vaccine.2017.11.008","journal-title":"Vaccine"},{"key":"337_CR29","doi-asserted-by":"publisher","first-page":"1917","DOI":"10.1016\/j.vaccine.2012.01.035","volume":"30","author":"JD Shearer","year":"2012","unstructured":"Shearer JD, Manetz TS, House RV (2012) Preclinical safety assessment of recombinant botulinum vaccine A\/B (rBV A\/B). Vaccine 30:1917\u20131926. https:\/\/doi.org\/10.1016\/j.vaccine.2012.01.035","journal-title":"Vaccine"},{"key":"337_CR30","doi-asserted-by":"publisher","first-page":"7313","DOI":"10.1016\/j.vaccine.2010.08.076","volume":"28","author":"JD Shearer","year":"2010","unstructured":"Shearer JD, Vassar ML, Swiderski W, Metcalfe K, Niemuth N, Henderson I (2010) Botulinum neurotoxin neutralizing activity of immune globulin (IG) purified from clinical volunteers vaccinated with recombinant botulinum vaccine (rBV A\/B). Vaccine 28:7313\u20137318. https:\/\/doi.org\/10.1016\/j.vaccine.2010.08.076","journal-title":"Vaccine"},{"key":"337_CR31","doi-asserted-by":"publisher","first-page":"755","DOI":"10.1080\/21645515.2018.1547613","volume":"15","author":"DY Shi","year":"2019","unstructured":"Shi DY, Chen BY, Mao YY, Zhou G, Lu JS, Yu YZ, Zhou XW, Sun ZW (2019) Development and evaluation of candidate subunit vaccine against botulinum neurotoxin serotype B. Hum Vaccin Immunother 15:755\u2013760. https:\/\/doi.org\/10.1080\/21645515.2018.1547613","journal-title":"Hum Vaccin Immunother"},{"key":"337_CR32","doi-asserted-by":"publisher","first-page":"2795","DOI":"10.1128\/IAI.01252-08","volume":"77","author":"C Shone","year":"2009","unstructured":"Shone C, Agostini H, Clancy J, Gu M, Yang HH, Chu Y, Johnson V, Taal M, McGlashan J, Brehm J, Tong X (2009) Bivalent recombinant vaccine for botulinum neurotoxin types A and B based on a polypeptide comprising their effector and translocation domains that is protective against the predominant A and B subtypes. Infect Immun 77:2795\u20132801. https:\/\/doi.org\/10.1128\/IAI.01252-08","journal-title":"Infect Immun"},{"key":"337_CR33","doi-asserted-by":"publisher","first-page":"2351","DOI":"10.1128\/JCM.26.11.2351-2356.1988","volume":"26","author":"LS Siegel","year":"1988","unstructured":"Siegel LS (1988) Human immune response to botulinum pentavalent (ABCDE) toxoid determined by a neutralization test and by an enzyme-linked immunosorbent assay. J Clin Microbiol 26:2351\u20132356. https:\/\/doi.org\/10.1128\/JCM.26.11.2351-2356.1988","journal-title":"J Clin Microbiol"},{"key":"337_CR34","doi-asserted-by":"publisher","unstructured":"Sundeen G, Barbieri JT (2017) Vaccines against Botulism Toxins 9 https:\/\/doi.org\/10.3390\/toxins9090268","DOI":"10.3390\/toxins9090268"},{"key":"337_CR35","doi-asserted-by":"publisher","unstructured":"Tehran DA, Pirazzini M (2018) Novel Botulinum Neurotoxins: Exploring Underneath the Iceberg Tip Toxins 10 https:\/\/doi.org\/10.3390\/toxins10050190","DOI":"10.3390\/toxins10050190"},{"key":"337_CR36","doi-asserted-by":"publisher","unstructured":"Tomic MT, Espinoza Y, Martinez Z, Pham K, Cobb RR, Snow DM, Earnhart CG, Pals T, Syar ES, Niemuth N, Kobs DJ, Farr-Jones S, Marks JD (2019) Monoclonal Antibody Combinations Prevent Serotype A and Serotype B Inhalational Botulism in a Guinea Pig Model Toxins 11 https:\/\/doi.org\/10.3390\/toxins11040208","DOI":"10.3390\/toxins11040208"},{"key":"337_CR37","doi-asserted-by":"publisher","first-page":"329","DOI":"10.1080\/21645515.2017.1405201","volume":"14","author":"CH Yu","year":"2018","unstructured":"Yu CH, Song DH, Choi JY, Joe HE, Jeong WH, Hur GH, Shin YK, Jeong ST (2018) A mutated recombinant subunit vaccine protects mice and guinea pigs against botulinum type A intoxication. Hum Vaccin Immunother 14:329\u2013336. https:\/\/doi.org\/10.1080\/21645515.2017.1405201","journal-title":"Hum Vaccin Immunother"},{"key":"337_CR38","doi-asserted-by":"publisher","first-page":"14130","DOI":"10.1038\/ncomms14130","volume":"8","author":"S Zhang","year":"2017","unstructured":"Zhang S, Masuyer G, Zhang J, Shen Y, Lundin D, Henriksson L, Miyashita SI, Mart\u00ednez-Carranza M, Dong M, Stenmark P (2017) Identification and characterization of a novel botulinum neurotoxin. Nat Commun 8:14130. https:\/\/doi.org\/10.1038\/ncomms14130","journal-title":"Nat Commun"}],"container-title":["Neurotoxicity Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s12640-021-00337-x.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s12640-021-00337-x\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s12640-021-00337-x.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,12]],"date-time":"2021-07-12T04:21:15Z","timestamp":1626063675000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s12640-021-00337-x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,2,22]]},"references-count":38,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2021,8]]}},"alternative-id":["337"],"URL":"https:\/\/doi.org\/10.1007\/s12640-021-00337-x","relation":{},"ISSN":["1029-8428","1476-3524"],"issn-type":[{"value":"1029-8428","type":"print"},{"value":"1476-3524","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,2,22]]},"assertion":[{"value":"31 October 2020","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"25 January 2021","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"27 January 2021","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"22 February 2021","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare that they have no conflict of interest.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of Interest"}},{"value":"All animal experiments were approved by the Institution of Animal Care and Use Committee of the Beijing Institute of Biotechnology and performed in accordance with the ethical guidelines of our institution and the National Institutes of Health guide for the Care and Use of Laboratory Animals.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical Approval"}}]}}