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It is an adaptor protein for the binding between Nedd4 of ubiquitin ligase (E3) and target substrate protein for ubiquitination. Northern blot analysis confirmed drastic increases in Ndfip1 mRNA in the striatum after METH injections, and in situ hybridization histochemistry showed that the mRNA expression was increased in the hippocampus and cerebellum at 2\u00a0h-2 days, in the cerebral cortex and striatum at 18\u00a0h-2 days after single METH administration. The knockdown of Ndfip1 expression with Ndfip1 siRNA significantly aggravated METH-induced neurotoxicity in the cultured monoaminergic neuronal cells. These results suggest that drastic increases in Ndfip1 mRNA is compensatory reaction to protect neurons against METH-induced neurotoxicity.<\/jats:p>","DOI":"10.1007\/s12640-024-00725-z","type":"journal-article","created":{"date-parts":[[2025,1,14]],"date-time":"2025-01-14T08:31:58Z","timestamp":1736843518000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Differentially Expressed Nedd4-binding Protein Ndfip1 Protects Neurons Against Methamphetamine-induced Neurotoxicity"],"prefix":"10.1007","volume":"43","author":[{"given":"Masato","family":"Asanuma","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ikuko","family":"Miyazaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean Lud","family":"Cadet","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,1,14]]},"reference":[{"issue":"5","key":"725_CR1","doi-asserted-by":"publisher","first-page":"667","DOI":"10.1172\/JCI5713","volume":"103","author":"H Abriel","year":"1999","unstructured":"Abriel H, Loffing J, Rebhun JF, Pratt JH, Schild L, Horisberger JD, Rotin D, Staub O (1999) Defective regulation of the epithelial Na\u2009+\u2009channel by Nedd4 in Liddle\u2019s syndrome. 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