{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T18:58:02Z","timestamp":1778698682546,"version":"3.51.4"},"reference-count":16,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,5,30]],"date-time":"2025-05-30T00:00:00Z","timestamp":1748563200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2025,5,30]],"date-time":"2025-05-30T00:00:00Z","timestamp":1748563200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Discov Onc"],"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:sec>\n            <jats:title>Objective<\/jats:title>\n            <jats:p>To examine the expression of non-SMC condensin II complex subunit D3 (NCAPD3) in papillary thyroid carcinoma (PTC) tissues, assess its impact on the growth and metastatic potential of PTC cells, and investigate its role in regulating glycolysis to uncover the underlying mechanisms involved.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods<\/jats:title>\n            <jats:p>NCAPD3 levels in PTC tissues were detected using immunohistochemistry. siRNA transfection was used to silence NCAPD3 in K1 and TPC-1 cells. Cell proliferation rates were detected using the Cell Counting Kit-8 assay, migration rates were evaluated using wound healing and Transwell cell migration assays, and invasion rates were assessed using the Transwell-Matrigel cell invasion assay. Moreover, the aerobic glycolysis-related factors lactate, lactate dehydrogenase A (LDHA), and pyruvate kinase M2 (PKM2) were detected using kits.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>NCAPD3 was highly expressed in PTC tissues. Its expression showed no significant association with patient age, gender, or lymphocytic thyroiditis but was significantly correlated with larger tumor size and lymphovascular invasion. NCAPD3 expression significantly decreased in K1 and TPC-1 cells after transfection with siRNA. Low NCAPD3 expression reduced the proliferation rate of K1 and TPC-1 cells and inhibited cell migration and invasion. Moreover, NCAPD3 silencing decreased LDHA, PKM2, and lactate levels.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>NCAPD3 was highly expressed in PTC tissues, and correlated with aggressive features (tumor size and lymphovascular invasion). NCAPD3 silencing inhibited proliferation, migration, invasion, and aerobic glycolysis of PTC cells. Therefore, NCAPD3 may serve as a potential therapeutic target for PTC.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1007\/s12672-025-02767-x","type":"journal-article","created":{"date-parts":[[2025,5,30]],"date-time":"2025-05-30T06:22:05Z","timestamp":1748586125000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["NCAPD3 is involved in papillary thyroid carcinoma proliferation, metastasis, and aerobic glycolytic pathway"],"prefix":"10.1007","volume":"16","author":[{"given":"Liubing","family":"Zhang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aiping","family":"Peng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yue","family":"Qin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,5,30]]},"reference":[{"key":"2767_CR1","doi-asserted-by":"publisher","first-page":"1252389","DOI":"10.3389\/fendo.2023.1252389","volume":"14","author":"B Song","year":"2023","unstructured":"Song B, et al. Global research landscape and trends of papillary thyroid cancer therapy: a bibliometric analysis. Front Endocrinol (Lausanne). 2023;14:1252389.","journal-title":"Front Endocrinol (Lausanne)."},{"key":"2767_CR2","doi-asserted-by":"crossref","unstructured":"Thewjitcharoen Y, et al. Impacts of the american joint committee on cancer (ajcc) 8th edition tumor, node, metastasis (tnm) staging system on outcomes of differentiated thyroid cancer in thai patients. Heliyon. 2021;7(3).","DOI":"10.1016\/j.heliyon.2021.e06624"},{"key":"2767_CR3","doi-asserted-by":"publisher","first-page":"22","DOI":"10.3390\/cancers15225414","volume":"15","author":"H Kim","year":"2023","unstructured":"Kim H, et al. Bilaterality as a risk factor for recurrence in papillary thyroid carcinoma. Cancers. 2023;15:22.","journal-title":"Cancers."},{"key":"2767_CR4","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s13046-022-02412-3","volume":"41","author":"Z Jing","year":"2022","unstructured":"Jing Z, et al. Ncapd3 enhances warburg effect through c-myc and e2f1 and promotes the occurrence and progression of colorectal cancer. J Exp Clin Cancer Res. 2022;41:1.","journal-title":"J Exp Clin Cancer Res"},{"key":"2767_CR5","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41420-023-01786-3","volume":"10","author":"Y Zhang","year":"2024","unstructured":"Zhang Y, et al. Ncapd3 exerts tumor-promoting effects in prostatic cancer via dual impact on mir-30a-5p by stat3-malat1 and myc. Cell Death Disc. 2024;10:1.","journal-title":"Cell Death Disc."},{"key":"2767_CR6","doi-asserted-by":"publisher","first-page":"110265","DOI":"10.1016\/j.cellsig.2022.110265","volume":"92","author":"Z Jing","year":"2022","unstructured":"Jing Z, et al. Ncapd3 promotes prostate cancer progression by up-regulating ezh2 and malat1 through stat3 and e2f1. 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Aerobic glycolysis promotes tumor immune evasion by hexokinase2-mediated phosphorylation of i\u03bab\u03b1. Cell Metab. 2022;34(9):1312-24.e6.","journal-title":"Cell Metab"},{"issue":"3","key":"2767_CR12","doi-asserted-by":"publisher","first-page":"221","DOI":"10.1631\/jzus.B2200221","volume":"24","author":"G Pan","year":"2023","unstructured":"Pan G, et al. Aerobic glycolysis in colon cancer is repressed by naringin via the hif1\u03b1 pathway. J Zhejiang Univ Sci B. 2023;24(3):221\u201331.","journal-title":"J Zhejiang Univ Sci B"},{"key":"2767_CR13","doi-asserted-by":"publisher","first-page":"21","DOI":"10.3390\/ijms242115787","volume":"24","author":"L Alberghina","year":"2023","unstructured":"Alberghina L. The warburg effect explained: Integration of enhanced glycolysis with heterogeneous mitochondria to promote cancer cell proliferation. 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