{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T17:01:59Z","timestamp":1781629319740,"version":"3.54.5"},"reference-count":24,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T00:00:00Z","timestamp":1767830400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T00:00:00Z","timestamp":1770163200000},"content-version":"vor","delay-in-days":27,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"Research Incubation Startup Fund of Jiangsu Province Geriatric Hospital","award":["FHQD202508"],"award-info":[{"award-number":["FHQD202508"]}]},{"name":"Natural Science Foundation of Jiangsu Health Vocational College","award":["jKCY 202413"],"award-info":[{"award-number":["jKCY 202413"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Discov Onc"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Purpose<\/jats:title>\n                    <jats:p>To elucidate the functional role of MCF2L antisense RNA1 (MCF2L-AS1) in colorectal cancer (CRC) progression and its potential molecular mechanism.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The expression of MCF2L-AS1\/MCF2L in CRC cell lines was detected by qRT-PCR. The biological function of MCF2L-AS1 was investigated in vitro and in vivo (colony formation, CCK8, Apoptosis assays, wound healing and transwell assays, and mouse xenograft models). Mechanistic investigations involved bioinformatics analysis followed by experimental validation using Fluorescence in situ hybridization, RNA pull-down, and RNA immunoprecipitation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>MCF2L-AS1 was notably upregulated in CRC tissues and cells. Functionally, MCF2L-AS1 suppression markedly attenuated the viability and colony capacity of CRC cells, and the number of migratory and invasive cells was markedly reduced both in vivo and in vitro. Mechanism studies have shown that MCF2L-AS1 binds to the heterogeneous nuclear ribonucleoprotein AUF1, facilitating MCF2L protein synthesis through a post-transcriptional regulatory mechanism independent of mRNA stability.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>MCF2L-AS1 promotes CRC progression through AUF1-dependent translational regulation of MCF2L expression. MCF2L-AS1 may represents a potential therapeutic target for clinical intervention in CRC.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1007\/s12672-026-04394-6","type":"journal-article","created":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T18:22:52Z","timestamp":1767896572000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["LncRNA MCF2L-AS1 promotes malignant progression of colorectal cancer by post-transcriptional activation of MCF2L"],"prefix":"10.1007","volume":"17","author":[{"given":"Hengchuan","family":"Shi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang","family":"Qiu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pei","family":"Tan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,1,8]]},"reference":[{"issue":"1","key":"4394_CR1","first-page":"7","volume":"72","author":"RL Siegel","year":"2022","unstructured":"Siegel RL, Miller KD, Fuchs HE, Jemal A. Cancer statistics, 2022. CA Cancer J Clin. 2022;72(1):7\u201333.","journal-title":"CA Cancer J Clin"},{"issue":"2\u20133","key":"4394_CR2","doi-asserted-by":"publisher","first-page":"135","DOI":"10.1007\/s10142-016-0524-x","volume":"17","author":"E Deniz","year":"2017","unstructured":"Deniz E, Erman B. Long noncoding RNA (lincRNA), a new paradigm in gene expression control. Funct Integr Genomics. 2017;17(2\u20133):135\u201343.","journal-title":"Funct Integr Genomics"},{"key":"4394_CR3","doi-asserted-by":"publisher","DOI":"10.1186\/s13045-014-0090-4","volume":"7","author":"G Loewen","year":"2014","unstructured":"Loewen G, Jayawickramarajah J, Zhuo Y, Shan B. Functions of lncRNA HOTAIR in lung cancer. J Hematol Oncol. 2014;7:90.","journal-title":"J Hematol Oncol"},{"issue":"2","key":"4394_CR4","doi-asserted-by":"publisher","first-page":"476","DOI":"10.1016\/j.canlet.2016.07.015","volume":"380","author":"J Kong","year":"2016","unstructured":"Kong J, Sun W, Li C, Wan L, Wang S, Wu Y, et al. Long non-coding RNA LINC01133 inhibits epithelial-mesenchymal transition and metastasis in colorectal cancer by interacting with SRSF6. Cancer Lett. 2016;380(2):476\u201384.","journal-title":"Cancer Lett"},{"issue":"6113","key":"4394_CR5","doi-asserted-by":"publisher","first-page":"1435","DOI":"10.1126\/science.1231776","volume":"338","author":"JT Lee","year":"2012","unstructured":"Lee JT. Epigenetic regulation by long noncoding RNAs. Science. 2012;338(6113):1435\u20139.","journal-title":"Science"},{"issue":"1","key":"4394_CR6","doi-asserted-by":"publisher","first-page":"7","DOI":"10.1038\/nrg3606","volume":"15","author":"A Fatica","year":"2014","unstructured":"Fatica A, Bozzoni I. Long non-coding RNAs: new players in cell differentiation and development. Nat Rev Genet. 2014;15(1):7\u201321.","journal-title":"Nat Rev Genet"},{"issue":"3","key":"4394_CR7","doi-asserted-by":"publisher","first-page":"215","DOI":"10.1016\/j.bbagrm.2013.10.009","volume":"1839","author":"S Nakagawa","year":"2014","unstructured":"Nakagawa S, Kageyama Y. Nuclear lncRNAs as epigenetic regulators-beyond skepticism. Biochim Biophys Acta. 2014;1839(3):215\u201322.","journal-title":"Biochim Biophys Acta"},{"key":"4394_CR8","doi-asserted-by":"publisher","DOI":"10.1186\/1741-7007-11-59","volume":"11","author":"AE Kornienko","year":"2013","unstructured":"Kornienko AE, Guenzl PM, Barlow DP, Pauler FM. Gene regulation by the act of long non-coding RNA transcription. BMC Biol. 2013;11:59.","journal-title":"BMC Biol"},{"issue":"1","key":"4394_CR9","doi-asserted-by":"publisher","DOI":"10.1186\/s13045-017-0449-4","volume":"10","author":"W Ni","year":"2017","unstructured":"Ni W, Zhang Y, Zhan Z, Ye F, Liang Y, Huang J, et al. A novel lncRNA uc.134 represses hepatocellular carcinoma progression by inhibiting CUL4A-mediated ubiquitination of LATS1. J Hematol Oncol. 2017;10(1):91.","journal-title":"J Hematol Oncol"},{"issue":"8","key":"4394_CR10","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-019-1804-x","volume":"10","author":"Y Zhao","year":"2019","unstructured":"Zhao Y, Du T, Du L, Li P, Li J, Duan W, et al. Long noncoding RNA LINC02418 regulates MELK expression by acting as a ceRNA and may serve as a diagnostic marker for colorectal cancer. Cell Death Dis. 2019;10(8):568.","journal-title":"Cell Death Dis"},{"issue":"1","key":"4394_CR11","doi-asserted-by":"publisher","first-page":"429","DOI":"10.1159\/000495239","volume":"51","author":"C Zhu","year":"2018","unstructured":"Zhu C, Cheng D, Qiu X, Zhuang M, Liu Z. Long noncoding RNA SNHG16 promotes cell proliferation by sponging microRNA-205 and upregulating ZEB1 expression in osteosarcoma. Cell Physiol Biochem. 2018;51(1):429\u201340.","journal-title":"Cell Physiol Biochem"},{"issue":"1","key":"4394_CR12","doi-asserted-by":"publisher","DOI":"10.1186\/s12943-018-0894-x","volume":"17","author":"M Xu","year":"2018","unstructured":"Xu M, Chen X, Lin K, Zeng K, Liu X, Pan B, et al. The long noncoding RNA SNHG1 regulates colorectal cancer cell growth through interactions with EZH2 and miR-154-5p. Mol Cancer. 2018;17(1):141.","journal-title":"Mol Cancer"},{"issue":"19","key":"4394_CR13","doi-asserted-by":"publisher","first-page":"4836","DOI":"10.7150\/thno.20942","volume":"7","author":"DL Chen","year":"2017","unstructured":"Chen DL, Lu YX, Zhang JX, Wei XL, Wang F, Zeng ZL, et al. Long non-coding RNA UICLM promotes colorectal cancer liver metastasis by acting as a ceRNA for microRNA-215 to regulate ZEB2 expression. Theranostics. 2017;7(19):4836\u201349.","journal-title":"Theranostics"},{"issue":"2","key":"4394_CR14","doi-asserted-by":"publisher","first-page":"263","DOI":"10.1093\/carcin\/bgaa093","volume":"42","author":"Z Zhang","year":"2021","unstructured":"Zhang Z, Yang W, Li N, Chen X, Ma F, Yang J, et al. LncRNA MCF2L-AS1 aggravates proliferation, invasion and glycolysis of colorectal cancer cells via the crosstalk with miR-874-3p\/FOXM1 signaling axis. Carcinogenesis. 2021;42(2):263\u201371.","journal-title":"Carcinogenesis"},{"key":"4394_CR15","doi-asserted-by":"publisher","first-page":"8685","DOI":"10.2147\/CMAR.S313905","volume":"13","author":"M Cai","year":"2021","unstructured":"Cai M, Hu W, Huang C, Zhou C, Li J, Chen Y, et al. lncRNA MCF2L-AS1\/miR-105\/ IL-1\u03b2 Axis Regulates Colorectal Cancer Cell Oxaliplatin Resistance. Cancer Manag Res. 2021;13:8685\u201394.","journal-title":"Cancer Manag Res"},{"issue":"3","key":"4394_CR16","doi-asserted-by":"publisher","first-page":"1527","DOI":"10.1111\/jcmm.13420","volume":"22","author":"QC Lu","year":"2018","unstructured":"Lu QC, Rui ZH, Guo ZL, Xie W, Shan S, Ren T. LncRNA-DANCR contributes to lung adenocarcinoma progression by sponging miR-496 to modulate mTOR expression. J Cell Mol Med. 2018;22(3):1527\u201337.","journal-title":"J Cell Mol Med"},{"issue":"4","key":"4394_CR17","doi-asserted-by":"publisher","first-page":"549","DOI":"10.1002\/wrna.1230","volume":"5","author":"AE Moore","year":"2014","unstructured":"Moore AE, Chenette DM, Larkin LC, Schneider RJ. Physiological networks and disease functions of RNA-binding protein AUF1. WIREs RNA. 2014;5(4):549\u201364.","journal-title":"WIREs RNA"},{"key":"4394_CR18","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms6248","volume":"5","author":"JH Yoon","year":"2014","unstructured":"Yoon JH, De S, Srikantan S, Abdelmohsen K, Grammatikakis I, Kim J, et al. PAR-CLIP analysis uncovers AUF1 impact on target RNA fate and genome integrity. Nat Commun. 2014;5:5248.","journal-title":"Nat Commun"},{"key":"4394_CR19","doi-asserted-by":"publisher","DOI":"10.1002\/wrna.1393","author":"EJ White","year":"2017","unstructured":"White EJ, Matsangos AE, Wilson GM. AUF1 regulation of coding and noncoding RNA. Wiley Interdiscip Rev RNA. 2017. https:\/\/doi.org\/10.1002\/wrna.1393.","journal-title":"Wiley Interdiscip Rev RNA"},{"key":"4394_CR20","doi-asserted-by":"publisher","DOI":"10.1186\/s12967-015-0637-3","volume":"13","author":"M Kumar","year":"2015","unstructured":"Kumar M, Matta A, Masui O, Srivastava G, Kaur J, Thakar A, et al. Nuclear heterogeneous nuclear ribonucleoprotein D is associated with poor prognosis and interactome analysis reveals its novel binding partners in oral cancer. J Transl Med. 2015;13:285.","journal-title":"J Transl Med"},{"issue":"5","key":"4394_CR21","doi-asserted-by":"publisher","first-page":"13437","DOI":"10.1080\/21655979.2022.2074108","volume":"13","author":"Q She","year":"2022","unstructured":"She Q, Chen Y, Liu H, Tan J, Li Y. A high level of the long non-coding RNA MCF2L-AS1 is associated with poor prognosis in breast cancer and MCF2L-AS1 activates YAP transcriptional activity to enhance breast cancer proliferation and metastasis. Bioengineered. 2022;13(5):13437\u201351.","journal-title":"Bioengineered"},{"issue":"6","key":"4394_CR22","doi-asserted-by":"publisher","DOI":"10.3802\/jgo.2022.33.e75","volume":"33","author":"Y Zhu","year":"2022","unstructured":"Zhu Y, Yang L, Wang J, Li Y, Chen Y. SP1-induced lncRNA MCF2L-AS1 promotes cisplatin resistance in ovarian cancer by regulating IGF2BP1\/IGF2\/MEK\/ERK axis. J Gynecol Oncol. 2022;33(6):e75.","journal-title":"J Gynecol Oncol"},{"issue":"13","key":"4394_CR23","doi-asserted-by":"publisher","first-page":"6100","DOI":"10.18632\/aging.204795","volume":"15","author":"H Ou","year":"2023","unstructured":"Ou H, Qian Y, Ma L. MCF2L-AS1 promotes the biological behaviors of hepatocellular carcinoma cells by regulating the miR-33a-5p\/FGF2 axis. Aging Albany NY. 2023;15(13):6100\u201316.","journal-title":"Aging Albany NY"},{"issue":"1","key":"4394_CR24","doi-asserted-by":"publisher","DOI":"10.1186\/s12885-021-08668-w","volume":"21","author":"W Kong","year":"2021","unstructured":"Kong W, Li H, Xie L, Cui G, Gu W, Zhang H, et al. LncRNA MCF2L-AS1 aggravates the malignant development of colorectal cancer via targeting miR-105-5p\/RAB22A axis. BMC Cancer. 2021;21(1):1069.","journal-title":"BMC Cancer"}],"container-title":["Discover Oncology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s12672-026-04394-6","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s12672-026-04394-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s12672-026-04394-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T16:20:33Z","timestamp":1781626833000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s12672-026-04394-6"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1,8]]},"references-count":24,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2026,12]]}},"alternative-id":["4394"],"URL":"https:\/\/doi.org\/10.1007\/s12672-026-04394-6","relation":{},"ISSN":["2730-6011"],"issn-type":[{"value":"2730-6011","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1,8]]},"assertion":[{"value":"13 March 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"5 January 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"8 January 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"All animal experiments were performed in compliance with a protocol approved by the Animal Care Committee of Nanjing Medical University. According to the ethical guidelines of our institution concerning animal research, the maximum allowable tumor diameter for mouse subcutaneous tumor models is set at 20\u00a0mm. Throughout the animal experiments, the maximum tumor diameter was not exceeded. The animal experiments were conducted following the ethical standards set by both institutional and national research committees, as well as adhering to the principles outlined in the 1964 Helsinki Declaration and its subsequent revisions. This research was conducted in accordance with the ARRIVE (Animal Research: Reporting of In Vivo Experiments) guidelines for reporting on animal studies.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"241"}}