{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T21:09:23Z","timestamp":1790975363490,"version":"4.1.0"},"reference-count":29,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2026,5,30]],"date-time":"2026-05-30T00:00:00Z","timestamp":1780099200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T00:00:00Z","timestamp":1785801600000},"content-version":"vor","delay-in-days":66,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Discov Onc"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Thyroid carcinoma (TC) presents a rising global incidence, with a subset of cases progressing aggressively despite standard therapies. The tumor microenvironment (TME), particularly the functional state of CD8\u207a T cells, is crucial in disease progression, yet a systematic, high-resolution understanding of the cellular interactions associated with immune exhaustion and tumor evolution in TC remains limited.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>To address this, we performed an integrated single-cell and spatial transcriptomic analysis of human TC samples. Our approach combined scRNA-seq data processing, cell communication inference, spatial co-localization validation, and machine learning-based prognostic modeling.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Result<\/jats:title>\n                    <jats:p>\n                      We identified a distinct tumor cell subcluster (Trem\/T01) characterized by\n                      <jats:italic>APOE<\/jats:italic>\n                      expression and a CD8\u207a exhausted T cell (Tex) subset. Computational and spatial analyses revealed a potential\n                      <jats:italic>APOE<\/jats:italic>\n                      (from Trem)-\n                      <jats:italic>NCF1<\/jats:italic>\n                      (from CD8\u207a Tex) interaction axis, with these cell types demonstrating significant spatial proximity in tumor tissues. Furthermore, we derived a prognostic gene signature from this network, constructing a robust risk stratification model validated across independent cohorts.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      This study uniquely delineates a specific tumor-immune interactive niche in TC, providing insights into potential mechanisms into immune evasion via the\n                      <jats:italic>APOE-NCF1<\/jats:italic>\n                      axis and delivering a translatable framework for patient prognostication.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1007\/s12672-026-05061-6","type":"journal-article","created":{"date-parts":[[2026,5,30]],"date-time":"2026-05-30T07:33:48Z","timestamp":1780126428000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Spatially resolved single cell analysis suggests an APOE NCF1 associated immunosuppressive niche and its prognostic signature in thyroid cancer"],"prefix":"10.1007","volume":"17","author":[{"given":"Han","family":"Su","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zheng","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nuo","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zimo","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renju","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziyi","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fen","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyue","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuyuan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chao","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,5,30]]},"reference":[{"key":"5061_CR1","doi-asserted-by":"crossref","unstructured":"Hu J et al. 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