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Stroke Res."],"published-print":{"date-parts":[[2020,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Platelet microvesicles (PMV) have previously been found elevated in acute ischemic stroke (IS) and could be biomarkers for risk of recurrence. PMV surface antigens such as P-selectin and phosphatidylserine (PS) reflect platelet activation and procoagulance. Tissue factor-positive microvesicles (TF<jats:sup>+<\/jats:sup>MV) are considered procoagulant, in particular if co-expressing PS. We enumerated MV subpopulations with these surface antigens in a cohort of 211 patients with primarily non-cardioembolic IS or transient ischemic attack (TIA) and investigated their association with long-term outcome. MV concentrations were determined by flow cytometry in the acute and convalescent phase. Primary outcome was a composite of fatal and non-fatal recurrent IS or myocardial infarction. Secondary outcomes were recurrent IS and all-cause mortality. Outcome events were obtained from Swedish registers during a follow-up of 1100 patient years. Concentrations of PS-positive and PS-negative MV populations were elevated in patients compared with healthy controls in both the acute and convalescent phase. PS<jats:sup>+<\/jats:sup>TF<jats:sup>+<\/jats:sup>PMV displayed pronounced elevations, median fold change 77 in the acute phase (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.0001) but were not associated with outcome, neither were PS<jats:sup>+<\/jats:sup>P-selectin<jats:sup>+<\/jats:sup>PMV. The only subpopulation positively associated with primary outcome was PS<jats:sup>\u2212<\/jats:sup>TF<jats:sup>+<\/jats:sup>PMV, with adjusted hazard ratio of 1.86 (1.04\u20133.31, <jats:italic>p<\/jats:italic>\u2009=\u20090.036) by Cox regression. Unexpectedly, several MV subpopulations tended to be associated with reduced risk of poor long-term outcome. Our results suggest that PS<jats:sup>+<\/jats:sup>TF<jats:sup>+<\/jats:sup>PMV may be a promising marker for cerebral ischemia, and that the in vivo generation of PS<jats:sup>\u2212<\/jats:sup>MV after IS\/TIA warrants further study. Future MV studies should ideally enumerate PS<jats:sup>+<\/jats:sup> and PS<jats:sup>\u2212<\/jats:sup>MV subpopulations separately.<\/jats:p>","DOI":"10.1007\/s12975-019-00777-w","type":"journal-article","created":{"date-parts":[[2020,2,4]],"date-time":"2020-02-04T10:53:53Z","timestamp":1580813633000},"page":"708-719","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Prognostic Value of Circulating Microvesicle Subpopulations in Ischemic Stroke and TIA"],"prefix":"10.1007","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1671-0827","authenticated-orcid":false,"given":"Annika","family":"Lundstr\u00f6m","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fariborz","family":"Mobarrez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisabeth","family":"Rooth","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charlotte","family":"Th\u00e5lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magnus","family":"von Arbin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Henriksson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruna","family":"Gigante","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ann-Charlotte","family":"Laska","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H\u00e5kan","family":"Wall\u00e9n","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,1,25]]},"reference":[{"issue":"10159","key":"777_CR1","doi-asserted-by":"crossref","first-page":"1859","DOI":"10.1016\/S0140-6736(18)32335-3","volume":"392","author":"DALYs GBD, Collaborators H","year":"2018","unstructured":"DALYs GBD, Collaborators H. 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