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It was found that BLE with proanthocyanidins (60\u00a0% of the total polyphenol content) increased the CD39-positive endothelial cell fraction (up to 10\u00a0% for 2.5\u00a0\u03bcg\/ml, and up to 33\u00a0% for 15\u00a0\u03bcg\/ml, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05 or less) in a concentration-dependent manner, and enhanced endothelial nitric oxide synthase (eNOS) activation (T495 phosphorylation decreased by 31\u2009\u00b1\u20096\u00a0% for 2.5\u00a0\u03bcg\/ml and 48\u2009\u00b1\u20096\u00a0% for 15\u00a0\u03bcg\/ml; S1177 phosphorylation increased by 13\u2009\u00b1\u20093\u00a0% for 2.5\u00a0\u03bcg\/ml and 18\u2009\u00b1\u20097\u00a0% for 15\u00a0\u03bcg\/ml, compared to untreated cells, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05 or less). Additionally, incubation for 24 or 48\u00a0h with BLE at a lower range of polyphenol concentrations, significantly increased cell viability with a maximal effect at 2.5\u00a0\u03bcg\/ml (viability increased by 24.8\u2009\u00b1\u20091.0\u00a0% for 24\u00a0h and by 32.5\u2009\u00b1\u20092.7\u00a0% for 48-h time incubation, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.0001). The increased CD39 expression and the increased eNOS activation in HUVEC can be regarded as the beneficial markers of the improvement of antiplatelet action of endothelial cells. Unexpectedly, these assumptions were not confirmed in the experimental model of platelet-endothelial cell interactions. These observations lead to the conclusion that BLE may improve endothelial cell viability at low physiological concentrations without affecting the antiplatelet action of endothelium.<\/jats:p>","DOI":"10.1007\/s13105-014-0370-z","type":"journal-article","created":{"date-parts":[[2014,11,21]],"date-time":"2014-11-21T11:24:29Z","timestamp":1416569069000},"page":"1007-1019","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Extract from Ribes nigrum leaves in vitro activates nitric oxide synthase (eNOS) and increases CD39 expression in human endothelial cells"],"prefix":"10.1007","volume":"70","author":[{"given":"Boguslawa","family":"Luzak","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magdalena","family":"Boncler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanna","family":"Rywaniak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominika","family":"Dudzinska","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marek","family":"Rozalski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Urszula","family":"Krajewska","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ewa","family":"Balcerczak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Podsedek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Malgorzata","family":"Redzynia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cezary","family":"Watala","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2014,11,19]]},"reference":[{"key":"370_CR1","doi-asserted-by":"publisher","first-page":"46637","DOI":"10.1074\/jbc.M405547200","volume":"279","author":"E Anter","year":"2004","unstructured":"Anter E, Thomas SR, Schulz E, Shapira OM, Vita JA, Keaney JF Jr (2004) Activation of endothelial nitric-oxide synthase by the p38 MAPK in response to black tea polyphenols. 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