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We aimed to characterize, in vitro, the contractile responses induced by Ang II, in jejunum from young (3\u20136 weeks old) and old rats (\u2265 1 year old), to evaluate possible functional differences associated to changes in receptor expression. Mechanical responses to Ang II were examined in vitro as changes in isometric tension. ATR expression was assessed by qRT-PCR. Ang II induced a contractile effect, antagonized by losartan, AT1R antagonist, and increased by PD123319, AT2R antagonist, as well by neural blocker \u03c9-conotoxin and by nitric oxide (NO) synthase inhibitor. No difference in the response was observed between young and old groups. AT1 receptor-mediated contractile response was decreased by U-73122, phospholipase C (PLC) inhibitor; or 2-aminoethoxy-diphenylborate (2-APB), inositol triphosphate (IP<jats:sub>3<\/jats:sub>) receptor inhibitor; or nifedipine, <jats:sc>l<\/jats:sc>-type calcium channel blocker. Age-related changes in the expression of both AT1 receptor subtypes, AT1a and AT1b, and of AT2 receptors were detected. In conclusion, Ang II modulates the spontaneous contractility of rat jejunum via postjunctional AT1 receptors, involving Ca<jats:sup>2+<\/jats:sup> mobilization from intracellular stores, via PLC\/IP<jats:sub>3<\/jats:sub> pathway, and Ca<jats:sup>2+<\/jats:sup> influx from extracellular space, via <jats:sc>l<\/jats:sc>-type channels. Prejunctional AT2 receptors would counteract AT1 receptor effects, via NO synthesis. The observed age-related differences in the expression of all AT receptor subtypes are not reflected in the muscular contractile response to Ang II.<\/jats:p>","DOI":"10.1007\/s13105-022-00892-7","type":"journal-article","created":{"date-parts":[[2022,4,8]],"date-time":"2022-04-08T09:07:55Z","timestamp":1649408875000},"page":"753-762","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Aging modifies receptor expression but not muscular contractile response to angiotensin II in rat jejunum"],"prefix":"10.1007","volume":"78","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1996-6529","authenticated-orcid":false,"given":"Maria Grazia","family":"Zizzo","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adele","family":"Cicio","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Federica","family":"Corrao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura","family":"Lentini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosa","family":"Serio","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2022,4,8]]},"reference":[{"key":"892_CR1","doi-asserted-by":"publisher","first-page":"600","DOI":"10.1161\/01.HYP.0000090323.58122.5C","volume":"42","author":"PM Abadir","year":"2003","unstructured":"Abadir PM, Carey RM, Siragy HM (2003) Angiotensin AT2 receptors directly stimulate renal NO in Bradykinin B2-receptor-null mice. 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