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'Mix D', Fishers base medium containing 2% v\/v Ultroser G serum alternative, showed the best combination of cell number increase and volumetric media cost reduction: 33% lower than 20% v\/v FBS DMEM. Further Nunc\u2122 EasYFlask\u2122 cell culture flasks\/CELLBIND\u00ae 6-well plates experiments showed that Mix D supported levels of Sol8 cell growth comparable to 20% v\/v FBS DMEM, but with lower levels of metabolic activity. Comparable growth in Mix D was observed for Sol8 cells that had undergone a period of adaptation to the serum-free medium, and clonally-derived Sol8 sub-populations. Mix D also matched serum-containing media performance in supporting growth of the porcine muscle cell line, pMYOSC, the porcine pre-adipocyte fat cell line, pADSC and the mouse pre-adipocyte cell line, 3T3 L1 in Nunc\u2122 EasYFlask\u2122 cell culture flasks\/CELLBIND\u00ae 6-well plates. 3T3 L1 cells cultivated in Mix D retained their capacity to differentiate into adipocytes, a cell type important for animal fat cultivation. The observation that Mix D supported growth of four different animal cell types at levels comparable to serum-containing medium recommends this formulation, and this repurposing screen approach, to researchers seeking serum alternatives.<\/jats:p>","DOI":"10.1007\/s13205-026-05091-5","type":"journal-article","created":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T04:08:14Z","timestamp":1792123694000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A repurposing screen of 29 media mixtures derived from 15 commercial serum-free media products identifies potential cost reductions for industrial animal cell cultivation"],"prefix":"10.1007","volume":"16","author":[{"given":"Matthew A. 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