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Neurons in the mouse V1 are tuned to light stimuli. Although the cellular information of the retina and the LGN has been widely studied, the transcriptome profiles of single light-stimulated neuron in V1 remain unknown. In our study, in vivo calcium imaging and whole-cell electrophysiological patch-clamp recording were utilized to identify 53 individual cells from layer 2\/3 of V1 as light-sensitive (LS) or non-light-sensitive (NS) by single-cell light-evoked calcium evaluation and action potential spiking. The contents of each cell after functional tests were aspirated\n                    <jats:italic>in vivo<\/jats:italic>\n                    through a patch-clamp pipette for mRNA sequencing. Moreover, the three-dimensional (3-D) morphological characterizations of the neurons were reconstructed in a live mouse after the whole-cell recordings. Our sequencing results indicated that V1 neurons with a high expression of genes related to transmission regulation, such as\n                    <jats:italic>Rtn4r<\/jats:italic>\n                    and\n                    <jats:italic>Rgs7,<\/jats:italic>\n                    and genes involved in membrane transport, such as Na\n                    <jats:sup>+<\/jats:sup>\n                    \/K\n                    <jats:sup>+<\/jats:sup>\n                    ATPase and NMDA-type glutamatergic receptors, preferentially responded to light stimulation. Furthermore, an antagonist that blocks\n                    <jats:italic>Rtn4r<\/jats:italic>\n                    signals could inactivate the neuronal responses to light stimulation in live mice. In conclusion, our findings of the\n                    <jats:italic>vivo<\/jats:italic>\n                    -seq analysis indicate the key role of the strength of synaptic transmission possesses neurons in V1 of light sensory.\n                  <\/jats:p>","DOI":"10.1007\/s13238-020-00720-y","type":"journal-article","created":{"date-parts":[[2020,4,29]],"date-time":"2020-04-29T06:03:00Z","timestamp":1588140180000},"page":"417-432","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Integrative analysis of in vivo recording with single-cell RNA-seq data reveals molecular properties of light-sensitive neurons in mouse V1"],"prefix":"10.1093","volume":"11","author":[{"given":"Jianwei","family":"Liu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengdi","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Le","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Na Clara","family":"Pan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changjiang","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junjing","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhentao","family":"Zuo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheng","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoqun","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2020,4,29]]},"reference":[{"key":"720_CR1","doi-asserted-by":"crossref","first-page":"C491","DOI":"10.1152\/ajpcell.00359.2015","volume":"310","author":"A Aperia","year":"2016","unstructured":"Aperia A, Akkuratov EE, Fontana JM, Brismar H (2016) Na+-K+-ATPase, a new class of plasma membrane receptors. 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