{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T06:07:53Z","timestamp":1777010873661,"version":"3.51.4"},"reference-count":40,"publisher":"Springer Science and Business Media LLC","issue":"12","license":[{"start":{"date-parts":[[2014,9,16]],"date-time":"2014-09-16T00:00:00Z","timestamp":1410825600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Tumor Biol."],"published-print":{"date-parts":[[2014,12]]},"DOI":"10.1007\/s13277-014-2566-9","type":"journal-article","created":{"date-parts":[[2014,9,15]],"date-time":"2014-09-15T10:59:57Z","timestamp":1410778797000},"page":"12473-12487","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Identification of a suitable qPCR reference gene in metastatic clear cell renal cell carcinoma"],"prefix":"10.1007","volume":"35","author":[{"given":"Piotr M.","family":"Wierzbicki","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jakub","family":"Klacz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Agnieszka","family":"Rybarczyk","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomasz","family":"Slebioda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcin","family":"Stanislawowski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Agata","family":"Wronska","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Kowalczyk","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcin","family":"Matuszewski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zbigniew","family":"Kmiec","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2014,9,16]]},"reference":[{"issue":"5","key":"2566_CR1","doi-asserted-by":"crossref","first-page":"1687","DOI":"10.1016\/j.juro.2007.01.065","volume":"177","author":"MF Dall\u2019Oglio","year":"2007","unstructured":"Dall\u2019Oglio MF, Arap MA, Antunes AA, Cury J, Leite KR, Srougi M. Impact of clinicopathological parameters in patients treated for renal cell carcinoma. J Urol. 2007;177(5):1687\u201391.","journal-title":"J Urol"},{"issue":"2","key":"2566_CR2","doi-asserted-by":"crossref","first-page":"430","DOI":"10.1016\/j.juro.2006.09.034","volume":"177","author":"G Novara","year":"2007","unstructured":"Novara G, Martignoni G, Artibani W, Ficarra V. Grading systems in renal cell carcinoma. J Urol. 2007;177(2):430\u20136.","journal-title":"J Urol"},{"issue":"4","key":"2566_CR3","doi-asserted-by":"crossref","first-page":"e60483","DOI":"10.1371\/journal.pone.0060483","volume":"8","author":"A Laird","year":"2013","unstructured":"Laird A, O\u2019Mahony FC, Nanda J, Riddick AC, O\u2019Donnell M, Harrison DJ, et al. Differential expression of prognostic proteomic markers in primary tumour, venous tumour thrombus and metastatic renal cell cancer tissue and correlation with patient outcome. PLoS One. 2013;8(4):e60483.","journal-title":"PLoS One"},{"issue":"5","key":"2566_CR4","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1007\/s40265-013-0043-1","volume":"73","author":"B Escudier","year":"2013","unstructured":"Escudier B, Albiges L, Sonpavde G. Optimal management of metastatic renal cell carcinoma: current status. Drugs. 2013;73(5):427\u201338.","journal-title":"Drugs"},{"issue":"11","key":"2566_CR5","doi-asserted-by":"crossref","first-page":"1063","DOI":"10.1038\/nmeth.2697","volume":"10","author":"SA Bustin","year":"2013","unstructured":"Bustin SA, Benes V, Garson J, Hellemans J, Huggett J, Kubista M, et al. The need for transparency and good practices in the qPCR literature. Nat Methods. 2013;10(11):1063\u20137.","journal-title":"Nat Methods"},{"key":"2566_CR6","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1186\/1471-2199-8-47","volume":"8","author":"M Jung","year":"2007","unstructured":"Jung M, Ramankulov A, Roigas J, Johannsen M, Ringsdorf M, Kristiansen G, et al. In search of suitable reference genes for gene expression studies of human renal cell carcinoma by real-time PCR. BMC Mol Biol. 2007;8:47.","journal-title":"BMC Mol Biol"},{"key":"2566_CR7","doi-asserted-by":"crossref","unstructured":"Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A et al. Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol. 2002; 3(7):RESEARCH0034.","DOI":"10.1186\/gb-2002-3-7-research0034"},{"issue":"9","key":"2566_CR8","doi-asserted-by":"crossref","first-page":"1475","DOI":"10.1038\/sj.bjc.6605851","volume":"103","author":"J Caradec","year":"2010","unstructured":"Caradec J, Sirab N, Revaud D, Keumeugni C, Loric S. Is GAPDH a relevant housekeeping gene for normalisation in colorectal cancer experiments? Br J Cancer. 2010;103(9):1475\u20136.","journal-title":"Br J Cancer"},{"issue":"3","key":"2566_CR9","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1007\/s12094-012-0924-x","volume":"15","author":"CM Guo","year":"2013","unstructured":"Guo CM, Liu SQ, Sun MZ. Novel insight into the role of GAPDH playing in tumor. Clin Transl Oncol. 2013;15(3):167\u201372.","journal-title":"Clin Transl Oncol"},{"issue":"4","key":"2566_CR10","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1097\/PDM.0b013e318212e0a9","volume":"20","author":"H Bjerregaard","year":"2011","unstructured":"Bjerregaard H, Pedersen S, Kristensen SR, Marcussen N. Reference genes for gene expression analysis by real-time reverse transcription polymerase chain reaction of renal cell carcinoma. Diagn Mol Pathol. 2011;20(4):212\u20137.","journal-title":"Diagn Mol Pathol"},{"issue":"1","key":"2566_CR11","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/j.ab.2010.09.010","volume":"408","author":"SM Gresner","year":"2011","unstructured":"Gresner SM, Golanska E, Kulczycka-Wojdala D, Jaskolski DJ, Papierz W, Liberski PP. Selection of reference genes for gene expression studies in astrocytomas. Anal Biochem. 2011;408(1):163\u20135.","journal-title":"Anal Biochem"},{"key":"2566_CR12","unstructured":"K\u0131l\u0131\u00e7 Y, \u00c7elebiler A\u00c7, Sak\u0131zl\u0131 M. Selecting housekeeping genes as references for the normalization of quantitative PCR data in breast cancer. Clin Transl Oncol. 2013;16(2):184\u2013190. doi:10.1007\/s12094-013-1058-57."},{"issue":"2","key":"2566_CR13","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1134\/S0026893311020129","volume":"45","author":"GS Krasnov","year":"2011","unstructured":"Krasnov GS, Oparina NY, Dmitriev AA, Kudryavtseva AV, Anedchenko EA, Kondrat\u2019eva TT. RPN1, a new reference gene for quantitative data normalization in lung and kidney cancer. Mol Biol. 2011;45(2):211\u201320.","journal-title":"Mol Biol"},{"issue":"5","key":"2566_CR14","doi-asserted-by":"crossref","first-page":"1915","DOI":"10.1016\/S0022-5347(05)00919-5","volume":"175","author":"F Ohl","year":"2006","unstructured":"Ohl F, Jung M, Radonic A, Sachs M, Loening SA, Jung K. Identification and validation of suitable endogenous reference genes for gene expression studies of human bladder cancer. J Urol. 2006;175(5):1915\u201320.","journal-title":"J Urol"},{"key":"2566_CR15","unstructured":"RefFinder. http:\/\/www.leonxie.com\/referencegene.php?type=reference . Accessed 20 Aug 2014."},{"issue":"6","key":"2566_CR16","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1023\/B:BILE.0000019559.84305.47","volume":"26","author":"MW Pfaffl","year":"2004","unstructured":"Pfaffl MW, Tichopad A, Prgomet C, Neuvians TP. Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper\u2014Excel-based tool using pair-wise correlations. Biotechnol Lett. 2004;26(6):509\u201315.","journal-title":"Biotechnol Lett"},{"issue":"15","key":"2566_CR17","doi-asserted-by":"crossref","first-page":"5245","DOI":"10.1158\/0008-5472.CAN-04-0496","volume":"64","author":"CL Andersen","year":"2004","unstructured":"Andersen CL, Jensen JL, Orntoft TF. Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets. Cancer Res. 2004;64(15):5245\u201350.","journal-title":"Cancer Res"},{"key":"2566_CR18","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1186\/1471-2199-7-33","volume":"7","author":"N Silver","year":"2006","unstructured":"Silver N, Best S, Jiang J, Thein SL. Selection of housekeeping genes for gene expression studies in human reticulocytes using real-time PCR. BMC Mol Biol. 2006;7:33.","journal-title":"BMC Mol Biol"},{"key":"2566_CR19","unstructured":"(CHMP) CfMPfHU. Guideline on bioanalytical method validation. In: Agency EM, editor. London: European Medicines Agency; 2011. p. 1\u201322."},{"issue":"4","key":"2566_CR20","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1373\/clinchem.2008.112797","volume":"55","author":"SA Bustin","year":"2009","unstructured":"Bustin SA, Benes V, Garson JA, Hellemans J, Huggett J, Kubista M, et al. The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin Chem. 2009;55(4):611\u201322.","journal-title":"Clin Chem"},{"key":"2566_CR21","author":"JR Dijkstra","year":"2014","unstructured":"Dijkstra JR, van Kempen LC, Nagtegaal ID, Bustin SA. Critical appraisal of quantitative PCR results in colorectal cancer research: can we rely on published qPCR results? Mol Oncol. 2014. doi: 10.1016\/j.molonc.2013.12.016 .","journal-title":"Mol Oncol"},{"issue":"6","key":"2566_CR22","doi-asserted-by":"crossref","first-page":"645","DOI":"10.4155\/bio.13.19","volume":"5","author":"P Amsterdam van","year":"2013","unstructured":"van Amsterdam P, Companjen A, Brudny-Kloeppel M, Golob M, Luedtke S, Timmerman P. The European Bioanalysis Forum community\u2019s evaluation, interpretation and implementation of the European Medicines Agency guideline on Bioanalytical Method Validation. Bioanalysis. 2013;5(6):645\u201359.","journal-title":"Bioanalysis"},{"issue":"6","key":"2566_CR23","doi-asserted-by":"crossref","first-page":"1037","DOI":"10.1038\/sj.bjc.6605573","volume":"102","author":"J Caradec","year":"2010","unstructured":"Caradec J, Sirab N, Keumeugni C, Moutereau S, Chimingqi M, Matar C, et al. \u2018Desperate house genes\u2019: the dramatic example of hypoxia. Br J Cancer. 2010;102(6):1037\u201343.","journal-title":"Br J Cancer"},{"issue":"4","key":"2566_CR24","doi-asserted-by":"crossref","first-page":"780","DOI":"10.1002\/cncr.24841","volume":"116","author":"AP Noon","year":"2010","unstructured":"Noon AP, Vlatkovic N, Polanski R, Maguire M, Shawki H, Parsons K, et al. p53 and MDM2 in renal cell carcinoma: biomarkers for disease progression and future therapeutic targets? Cancer. 2010;116(4):780\u201390.","journal-title":"Cancer"},{"issue":"15","key":"2566_CR25","doi-asserted-by":"crossref","first-page":"6498","DOI":"10.1158\/0008-5472.CAN-05-0017","volume":"65","author":"HE Warburton","year":"2005","unstructured":"Warburton HE, Brady M, Vlatkovic N, Linehan WM, Parsons K, Boyd MT. p53 regulation and function in renal cell carcinoma. Cancer Res. 2005;65(15):6498\u2013503.","journal-title":"Cancer Res"},{"key":"2566_CR26","unstructured":"Glenn ST, Jones CA, Liang P, Kaushik D, Gross KW, Kim HL. Expression profiling of archival renal tumors by quantitative PCR to validate prognostic markers. Biotechniques. 2007; 43(5):639\u201340, 42\u20133, 47."},{"key":"2566_CR27","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1186\/1471-2199-15-9","volume":"15","author":"S Dupasquier","year":"2014","unstructured":"Dupasquier S, Delmarcelle AS, Marbaix E, Cosyns JP, Courtoy PJ, Pierreux CE. Validation of housekeeping gene and impact on normalized gene expression in clear cell renal cell carcinoma: critical reassessment of YBX3\/ZONAB\/CSDA expression. BMC Mol Biol. 2014;15:9.","journal-title":"BMC Mol Biol"},{"issue":"2","key":"2566_CR28","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1016\/j.ab.2011.06.009","volume":"417","author":"Z Wotschofsky","year":"2011","unstructured":"Wotschofsky Z, Meyer HA, Jung M, Fendler A, Wagner I, Stephan C, et al. Reference genes for the relative quantification of microRNAs in renal cell carcinomas and their metastases. Anal Biochem. 2011;417(2):233\u201341.","journal-title":"Anal Biochem"},{"issue":"7","key":"2566_CR29","doi-asserted-by":"crossref","first-page":"e66047","DOI":"10.1371\/journal.pone.0066047","volume":"8","author":"Y Gokmen-Polar","year":"2013","unstructured":"Gokmen-Polar Y, Cook RW, Goswami CP, Wilkinson J, Maetzold D, Stone JF, et al. A gene signature to determine metastatic behavior in thymomas. PLoS One. 2013;8(7):e66047.","journal-title":"PLoS One"},{"issue":"Suppl 4","key":"2566_CR30","first-page":"63","volume":"60","author":"PM Wierzbicki","year":"2009","unstructured":"Wierzbicki PM, Adrych K, Kartanowicz D, Dobrowolski S, Stanislawowski M, Chybicki J, et al. Fragile histidine triad (FHIT) gene is overexpressed in colorectal cancer. J Physiol Pharmacol. 2009;60 Suppl 4:63\u201370.","journal-title":"J Physiol Pharmacol"},{"issue":"27","key":"2566_CR31","doi-asserted-by":"crossref","first-page":"4363","DOI":"10.3748\/wjg.v19.i27.4363","volume":"19","author":"PM Wierzbicki","year":"2013","unstructured":"Wierzbicki PM, Adrych K, Kartanowicz D, Stanislawowski M, Kowalczyk A, Godlewski J, et al. Underexpression of LATS1 TSG in colorectal cancer is associated with promoter hypermethylation. World J Gastroenterol. 2013;19(27):4363\u201373.","journal-title":"World J Gastroenterol"},{"key":"2566_CR32","doi-asserted-by":"crossref","unstructured":"Nag S, Zhang X, Srivenugopal KS, Wang MH, Wang W, Zhang R. Targeting MDM2-p53 interaction for cancer therapy: are we there yet? Curr Med Chem. 2013;21(5):553\u2013574.","DOI":"10.2174\/09298673113206660325"},{"issue":"4","key":"2566_CR33","doi-asserted-by":"crossref","first-page":"644","DOI":"10.1016\/j.eururo.2011.06.041","volume":"60","author":"M Sun","year":"2011","unstructured":"Sun M, Shariat SF, Cheng C, Ficarra V, Murai M, Oudard S, et al. Prognostic factors and predictive models in renal cell carcinoma: a contemporary review. Eur Urol. 2011;60(4):644\u201361.","journal-title":"Eur Urol"},{"issue":"6","key":"2566_CR34","doi-asserted-by":"crossref","first-page":"711","DOI":"10.1586\/era.13.57","volume":"13","author":"B Shuch","year":"2013","unstructured":"Shuch B, Linehan WM, Srinivasan R. Aerobic glycolysis: a novel target in kidney cancer. Expert Rev Anticancer Ther. 2013;13(6):711\u20139.","journal-title":"Expert Rev Anticancer Ther"},{"issue":"4","key":"2566_CR35","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1016\/j.urology.2003.11.011","volume":"63","author":"R Zigeuner","year":"2004","unstructured":"Zigeuner R, Ratschek M, Rehak P, Schips L, Langner C. Value of p53 as a prognostic marker in histologic subtypes of renal cell carcinoma: a systematic analysis of primary and metastatic tumor tissue. Urology. 2004;63(4):651\u20135.","journal-title":"Urology"},{"issue":"1","key":"2566_CR36","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1006\/abio.1993.1558","volume":"215","author":"M Block De","year":"1993","unstructured":"De Block M, Debrouwer D. RNA-RNA in situ hybridization using digoxigenin-labeled probes: the use of high-molecular-weight polyvinyl alcohol in the alkaline phosphatase indoxyl-nitroblue tetrazolium reaction. Anal Biochem. 1993;215(1):86\u20139.","journal-title":"Anal Biochem"},{"issue":"1","key":"2566_CR37","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1038\/aps.2009.178","volume":"31","author":"F Zhang","year":"2010","unstructured":"Zhang F, Wang ZM, Liu HY, Bai Y, Wei S, Li Y, et al. Application of RT-PCR in formalin-fixed and paraffin-embedded lung cancer tissues. Acta Pharmacol Sin. 2010;31(1):111\u20137.","journal-title":"Acta Pharmacol Sin"},{"issue":"1","key":"2566_CR38","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1016\/j.ab.2009.07.022","volume":"394","author":"YL Li","year":"2009","unstructured":"Li YL, Ye F, Hu Y, Lu WG, Xie X. Identification of suitable reference genes for gene expression studies of human serous ovarian cancer by real-time polymerase chain reaction. Anal Biochem. 2009;394(1):110\u20136.","journal-title":"Anal Biochem"},{"issue":"6","key":"2566_CR39","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1016\/j.jalz.2009.11.002","volume":"6","author":"FM Gebhardt","year":"2010","unstructured":"Gebhardt FM, Scott HA, Dodd PR. Housekeepers for accurate transcript expression analysis in Alzheimer\u2019s disease autopsy brain tissue. Alzheimers Dement. 2010;6(6):465\u201374.","journal-title":"Alzheimers Dement"},{"key":"2566_CR40","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1186\/1471-2199-11-61","volume":"11","author":"KM Curtis","year":"2010","unstructured":"Curtis KM, Gomez LA, Rios C, Garbayo E, Raval AP, Perez-Pinzon MA, et al. EF1alpha and RPL13a represent normalization genes suitable for RT-qPCR analysis of bone marrow derived mesenchymal stem cells. BMC Mol Biol. 2010;11:61.","journal-title":"BMC Mol Biol"}],"container-title":["Tumor Biology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s13277-014-2566-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s13277-014-2566-9\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s13277-014-2566-9","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,8,15]],"date-time":"2019-08-15T00:11:38Z","timestamp":1565827898000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s13277-014-2566-9"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,9,16]]},"references-count":40,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2014,12]]}},"alternative-id":["2566"],"URL":"https:\/\/doi.org\/10.1007\/s13277-014-2566-9","relation":{},"ISSN":["1010-4283","1423-0380"],"issn-type":[{"value":"1010-4283","type":"print"},{"value":"1423-0380","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,9,16]]}}}