{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T22:58:01Z","timestamp":1792709881111,"version":"4.5.0"},"reference-count":30,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2016,11,24]],"date-time":"2016-11-24T00:00:00Z","timestamp":1479945600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2016,11,24]],"date-time":"2016-11-24T00:00:00Z","timestamp":1479945600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/100006545","name":"National Institute on Minority Health and Health Disparities","doi-asserted-by":"publisher","award":["5P20MD006988"],"award-info":[{"award-number":["5P20MD006988"]}],"id":[{"id":"10.13039\/100006545","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Universidad de Guadalajara","award":["UDG-CA-703"],"award-info":[{"award-number":["UDG-CA-703"]}]},{"name":"Universidad de Guadalajara","award":["UDG-CA-701"],"award-info":[{"award-number":["UDG-CA-701"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Autoimmun Highlights"],"published-print":{"date-parts":[[2017,12]]},"DOI":"10.1007\/s13317-016-0089-7","type":"journal-article","created":{"date-parts":[[2016,11,24]],"date-time":"2016-11-24T07:22:12Z","timestamp":1479972132000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Detection of autoantibodies to DSF70\/LEDGFp75 in Mexican Hispanics using multiple complementary assay platforms"],"prefix":"10.1007","volume":"8","author":[{"given":"M\u00f3nica","family":"V\u00e1zquez-Del Mercado","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eduardo","family":"G\u00f3mez-Ba\u00f1uelos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosa Elena","family":"Navarro-Hern\u00e1ndez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oscar","family":"Pizano-Martinez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adan","family":"Salda\u00f1a-Mill\u00e1n","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Efrain","family":"Chavarria-Avila","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lorena","family":"Gonzalez-Rosas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lilia","family":"Andrade-Ortega","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miguel Angel","family":"Saavedra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olga Lidia","family":"Vera-Lastra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis Javier","family":"Jara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel","family":"Medrano-Ram\u00edrez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"Cruz-Reyes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ignacio","family":"Garc\u00eda-De la Torre","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Escarra-Senmarti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisiane Maria Enriconi-Dos","family":"Anjos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anamika","family":"Basu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roger","family":"Albesa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Mahler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos A.","family":"Casiano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2016,11,24]]},"reference":[{"key":"89_CR1","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1111\/j.1749-6632.1997.tb52040.x","volume":"815","author":"EM Tan","year":"1997","unstructured":"Tan EM (1997) Autoantibodies and autoimmunity: a three-decade perspective. A tribute to Henry G. Kunkel. Ann N Y Acad Sci 815:1\u201314","journal-title":"Ann N Y Acad Sci"},{"key":"89_CR2","first-page":"2144","volume":"32","author":"A Dellavance","year":"2005","unstructured":"Dellavance A, Viana VS, Leon EP et al (2005) The clinical spectrum of antinuclear antibodies associated with the nuclear dense fine speckled immunofluorescence pattern. J Rheumatol 32:2144\u20132149","journal-title":"J Rheumatol"},{"key":"89_CR3","doi-asserted-by":"publisher","first-page":"703759","DOI":"10.1155\/2013\/703759","volume":"2013","author":"M Miyara","year":"2013","unstructured":"Miyara M, Albesa R, Charuel JL et al (2013) Clinical phenotypes of patients with anti-DFS70\/LEDGF antibodies in a routine ANA referral cohort. Clin Dev Immunol 2013:703759","journal-title":"Clin Dev Immunol"},{"key":"89_CR4","doi-asserted-by":"publisher","DOI":"10.1007\/s12016-016-8564-5","author":"K Conrad","year":"2016","unstructured":"Conrad K, Rober N, Andrade LE et al (2016) The clinical relevance of anti-DFS70 autoantibodies. Clin Rev Allergy Immunol. doi:\n                    10.1007\/s12016-016-8564-5","journal-title":"Clin Rev Allergy Immunol"},{"key":"89_CR5","doi-asserted-by":"publisher","first-page":"198","DOI":"10.1016\/j.autrev.2015.11.006","volume":"15","author":"M Mahler","year":"2016","unstructured":"Mahler M, Meroni PL, Andrade LE et al (2016) Towards a better understanding of the clinical association of anti-DFS70 autoantibodies. Autoimmun Rev 15:198\u2013201","journal-title":"Autoimmun Rev"},{"key":"89_CR6","doi-asserted-by":"publisher","first-page":"1211","DOI":"10.1067\/mai.2000.107039","volume":"105","author":"RL Ochs","year":"2000","unstructured":"Ochs RL, Muro Y, Si Y et al (2000) Autoantibodies to DFS70 kd\/transcription coactivator p75 in atopic dermatitis and other conditions. J Allergy Clin Immunol 105:1211\u20131220","journal-title":"J Allergy Clin Immunol"},{"key":"89_CR7","doi-asserted-by":"publisher","first-page":"241","DOI":"10.1016\/j.clim.2015.07.014","volume":"161","author":"A Basu","year":"2015","unstructured":"Basu A, Woods-Burnham L, Ortiz G et al (2015) Specificity of antinuclear autoantibodies recognizing the dense fine speckled nuclear pattern: preferential targeting of DFS70\/LEDGFp75 over its interacting partner MeCP2. Clin Immunol 161:241\u2013250","journal-title":"Clin Immunol"},{"key":"89_CR8","doi-asserted-by":"publisher","first-page":"273","DOI":"10.1007\/s10238-015-0367-0","volume":"16","author":"RL Ochs","year":"2016","unstructured":"Ochs RL, Mahler M, Basu A et al (2016) The significance of autoantibodies to DFS70\/LEDGFp75 in health and disease: integrating basic science with clinical understanding. Clin Exp Med 16:273\u2013293","journal-title":"Clin Exp Med"},{"key":"89_CR9","doi-asserted-by":"publisher","first-page":"116","DOI":"10.3389\/fimmu.2015.00116","volume":"6","author":"A Basu","year":"2015","unstructured":"Basu A, Sanchez TW, Casiano CA (2015) DFS70\/LEDGFp75: an enigmatic autoantigen at the interface between autoimmunity, AIDS, and cancer. Front Immunol 6:116","journal-title":"Front Immunol"},{"key":"89_CR10","doi-asserted-by":"publisher","first-page":"108","DOI":"10.1016\/j.tibs.2014.12.001","volume":"40","author":"Z Debyser","year":"2015","unstructured":"Debyser Z, Christ F, De Rijck J et al (2015) Host factors for retroviral integration site selection. Trends Biochem Sci 40:108\u2013116","journal-title":"Trends Biochem Sci"},{"key":"89_CR11","doi-asserted-by":"publisher","first-page":"92","DOI":"10.3899\/jrheum.121168","volume":"40","author":"Y Muro","year":"2013","unstructured":"Muro Y, Sugiura K, Nakashima R et al (2013) Low prevalence of anti-DFS70\/LEDGF antibodies in patients with dermatomyositis and other systemic autoimmune rheumatic diseases. J Rheumatol 40:92\u201393","journal-title":"J Rheumatol"},{"key":"89_CR12","doi-asserted-by":"publisher","first-page":"191","DOI":"10.1002\/art.30084","volume":"63","author":"HA Mariz","year":"2011","unstructured":"Mariz HA, Sato EI, Barbosa SH et al (2011) Pattern on the antinuclear antibody-HEp-2 test is a critical parameter for discriminating antinuclear antibody-positive healthy individuals and patients with autoimmune rheumatic diseases. Arthritis Rheum 63:191\u2013200","journal-title":"Arthritis Rheum"},{"key":"89_CR13","doi-asserted-by":"publisher","first-page":"892","DOI":"10.1002\/art.20096","volume":"50","author":"A Watanabe","year":"2004","unstructured":"Watanabe A, Kodera M, Sugiura K et al (2004) Anti-DFS70 antibodies in 597 healthy hospital workers. Arthritis Rheum 50:892\u2013900","journal-title":"Arthritis Rheum"},{"key":"89_CR14","doi-asserted-by":"publisher","first-page":"2104","DOI":"10.3899\/jrheum.120598","volume":"39","author":"M Mahler","year":"2012","unstructured":"Mahler M, Parker T, Peebles CL et al (2012) Anti-DFS70\/LEDGF antibodies are more prevalent in healthy individuals compared to patients with systemic autoimmune rheumatic diseases. J Rheumatol 39:2104\u20132110","journal-title":"J Rheumatol"},{"key":"89_CR15","doi-asserted-by":"publisher","first-page":"499","DOI":"10.7754\/Clin.Lab.2015.150905","volume":"62","author":"CA Seelig","year":"2016","unstructured":"Seelig CA, Bauer O, Seelig HP (2016) Autoantibodies against DFS70\/LEDGF exclusion markers for systemic autoimmune rheumatic diseases (SARD). Clin Lab 62:499\u2013517","journal-title":"Clin Lab"},{"key":"89_CR16","doi-asserted-by":"publisher","first-page":"e1706","DOI":"10.1542\/peds.2013-3914","volume":"134","author":"M Fabris","year":"2014","unstructured":"Fabris M, Zago S, Tosolini R et al (2014) Anti-DFS70 antibodies: a useful biomarker in a pediatric case with suspected autoimmune disease. Pediatrics 134:e1706\u2013e1708","journal-title":"Pediatrics"},{"key":"89_CR17","doi-asserted-by":"publisher","DOI":"10.1007\/s12026-016-8836-4","author":"M Infantino","year":"2016","unstructured":"Infantino M, Meacci F, Grossi V et al (2016) The clinical impact of Anti-DFS70 antibodies in undifferentiated connective tissue disease: case reports and a review of the literature. Immunol Res. doi:\n                    10.1007\/s12026-016-8836-4","journal-title":"Immunol Res"},{"key":"89_CR18","doi-asserted-by":"publisher","first-page":"797","DOI":"10.1177\/0961203316640920","volume":"25","author":"EK Chan","year":"2016","unstructured":"Chan EK, Damoiseaux J, de Melo Cruvinel W et al (2016) Report on the second International Consensus on ANA Pattern (ICAP) workshop in Dresden 2015. Lupus 25:797\u2013804","journal-title":"Lupus"},{"key":"89_CR19","doi-asserted-by":"publisher","first-page":"915","DOI":"10.1038\/sj.cdd.4401063","volume":"9","author":"X Wu","year":"2002","unstructured":"Wu X, Daniels T, Molinaro C et al (2002) Caspase cleavage of the nuclear autoantigen LEDGF\/p75 abrogates its pro-survival function: implications for autoimmunity in atopic disorders. Cell Death Differ 9:915\u2013925","journal-title":"Cell Death Differ"},{"key":"89_CR20","doi-asserted-by":"publisher","first-page":"1293","DOI":"10.1158\/1541-7786.MCR-08-0125","volume":"6","author":"TA Brown-Bryan","year":"2008","unstructured":"Brown-Bryan TA, Leoh LS, Ganapathy V et al (2008) Alternative splicing and caspase-mediated cleavage generate antagonistic variants of the stress oncoprotein LEDGF\/p75. Mol Cancer Res 6:1293\u20131307","journal-title":"Mol Cancer Res"},{"key":"89_CR21","doi-asserted-by":"publisher","first-page":"8","DOI":"10.1007\/s13317-016-0081-2","volume":"7","author":"C Bentow","year":"2016","unstructured":"Bentow C, Fritzler MJ, Mummert E et al (2016) Recognition of the dense fine speckled (DFS) pattern remains challenging: results from an international internet-based survey. Auto Immun Highlights 7:8","journal-title":"Auto Immun Highlights"},{"key":"89_CR22","doi-asserted-by":"publisher","first-page":"50","DOI":"10.1016\/j.jim.2016.06.008","volume":"436","author":"N Bizzaro","year":"2016","unstructured":"Bizzaro N, Pesente F, Cucchiaro F et al (2016) Anti-DFS70 antibodies detected by immunoblot methods: a reliable tool to confirm the dense fine speckles ANA pattern. J Immunol Methods 436:50\u201353","journal-title":"J Immunol Methods"},{"key":"89_CR23","doi-asserted-by":"publisher","first-page":"897","DOI":"10.1177\/0961203316641773","volume":"25","author":"C Bentow","year":"2016","unstructured":"Bentow C, Rosenblum R, Correia P et al (2016) Development and multi-center evaluation of a novel immunoadsorption method for anti-DFS70 antibodies. Lupus 25:897\u2013904","journal-title":"Lupus"},{"key":"89_CR24","doi-asserted-by":"publisher","first-page":"6","DOI":"10.5114\/ceji.2016.58812","volume":"41","author":"E Mutlu","year":"2016","unstructured":"Mutlu E, Eyig\u00f6r M, Mutlu D et al (2016) Confirmation of anti-DFS70 antibodies is needed in routine clinical samples with DFS staining pattern. Cent Eur J Immunol 41:6\u201311","journal-title":"Cent Eur J Immunol"},{"key":"89_CR25","doi-asserted-by":"publisher","first-page":"271","DOI":"10.1016\/j.cca.2015.10.008","volume":"451","author":"N Bizzaro","year":"2015","unstructured":"Bizzaro N, Tonutti E, Tampoia M et al (2015) Specific chemoluminescence and immunoadsorption tests for anti-DFS70 antibodies avoid false positive results by indirect immunofluorescence. Clin Chim Acta 451:271\u2013277","journal-title":"Clin Chim Acta"},{"key":"89_CR26","doi-asserted-by":"publisher","first-page":"787","DOI":"10.1177\/0961203316644337","volume":"25","author":"K Conrad","year":"2016","unstructured":"Conrad K, Andrade LE, Chan EK et al (2016) From autoantibody research to standardized diagnostic assays in the management of human diseases\u2014report of the 12th Dresden Symposium on Autoantibodies. Lupus 25:787\u2013796","journal-title":"Lupus"},{"key":"89_CR27","doi-asserted-by":"publisher","first-page":"10","DOI":"10.1007\/s13317-016-0082-1","volume":"7","author":"S Gund\u00edn","year":"2016","unstructured":"Gund\u00edn S, Irure-Ventura J, Asensio E et al (2016) Measurement of anti-DFS70 antibodies in patients with ANA-associated autoimmune rheumatic diseases suspicion is cost-effective. Auto Immun Highlights 7:10","journal-title":"Auto Immun Highlights"},{"key":"89_CR28","doi-asserted-by":"publisher","first-page":"161","DOI":"10.1016\/S0165-2478(01)00243-7","volume":"78","author":"K Yamada","year":"2001","unstructured":"Yamada K, Senju S, Shinohara T et al (2001) Humoral immune response directed against LEDGF in patients with VKH. Immunol Lett 78:161\u2013168","journal-title":"Immunol Lett"},{"key":"89_CR29","doi-asserted-by":"publisher","first-page":"266","DOI":"10.1111\/j.1442-200X.2011.03514.x","volume":"54","author":"Y Itoh","year":"2012","unstructured":"Itoh Y, Shigemori T, Igarashi T et al (2012) Fibromyalgia and chronic fatigue syndrome in children. Pediatr Int 54:266\u2013271","journal-title":"Pediatr Int"},{"key":"89_CR30","doi-asserted-by":"publisher","first-page":"14","DOI":"10.1002\/pros.20112","volume":"62","author":"T Daniels","year":"2005","unstructured":"Daniels T, Zhang J, Gutierrez I et al (2005) Antinuclear autoantibodies in PCa: immunity to LEDGF\/p75, a survival protein highly expressed in prostate tumors and cleaved during apoptosis. Prostate 62:14\u201326","journal-title":"Prostate"}],"container-title":["Autoimmunity Highlights"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s13317-016-0089-7\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s13317-016-0089-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s13317-016-0089-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,14]],"date-time":"2020-05-14T10:31:54Z","timestamp":1589452314000},"score":1,"resource":{"primary":{"URL":"https:\/\/autoimmunhighlights.biomedcentral.com\/articles\/10.1007\/s13317-016-0089-7"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,11,24]]},"references-count":30,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2017,12]]}},"alternative-id":["89"],"URL":"https:\/\/doi.org\/10.1007\/s13317-016-0089-7","relation":{},"ISSN":["2038-0305","2038-3274"],"issn-type":[{"value":"2038-0305","type":"print"},{"value":"2038-3274","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,11,24]]},"assertion":[{"value":"7 November 2016","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"17 November 2016","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"24 November 2016","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Compliance with ethical standards"}},{"value":"CAC received funding from the National Institutes of Health (NIH) through grant 5P20MD006988. MVDM received funding from the University of Guadalajara through grants UDG-CA-703 (Immunology and Rheumatology) and UDG-CA-701 (Aging, Immunometabolism, and Oxidative Stress).","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Funding"}},{"value":"Dr. Ignacio Garcia De La Torre is an employee of Immuno-Mex (Guadalajara, Mexico), a company that distributes reagents and equipment for clinical and diagnostic laboratories. Dr. Michael Mahler and Mr. Roger Albesa are employees of Inova Diagnostics (San Diego, USA), a company that specializes on the development of autoimmune technologies and diagnostic markers used in clinical laboratories and hospitals. For the remaining authors none were declared.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and\/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. The studies with human subjects presented in this article were approved by the Institutional Review Boards of the University of Guadalajara and Loma Linda University. This article does not contain any studies with animals performed by any of the authors.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Research involving human subjects and animals"}},{"value":"Informed consent was obtained from all individual participants included in this study.","order":5,"name":"Ethics","group":{"name":"EthicsHeading","label":"Informed consent"}}],"article-number":"1"}}