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Retinal imaging including optical coherence tomography (OCT) angiography (OCTA) and\n                    <jats:italic>en face<\/jats:italic>\n                    null OCT was performed in most dogs at 5- and 9-weeks PI. Following termination, eyes were examined histologically. Ex vivo and in vivo SRI could be performed in 7 adult eyes including in that of a dog with end stage retinal degeneration. SRI of AAV2\/5-CBA-tdTomato-WPRE was also successful in all 13 eyes including those of juvenile animals, yet this frequently required multiple attempts and\/or using custom-modified devices that were adjusted to the smaller curvature of the ocular globes. Localized tdTomato expression was found in 11\/13 eyes along the cannula path in the suprachoroidal space (SCS) in the sclera and choroid. Clinical and cellular signs of inflammation were seen in 7\/13 eyes. The Orbit\n                    <jats:sup>\u00ae<\/jats:sup>\n                    SDS device allows for controlled subretinal injections via a posterior approach in adult and juvenile dogs. There was variable vector reflux from the subretinal bleb and\/or leakage from the device into the SCS. A cellular immune reaction to the AAV capsid\/transgene may have contributed to retinal and choroidal inflammation.\n                  <\/jats:p>","DOI":"10.1007\/s13346-025-01929-2","type":"journal-article","created":{"date-parts":[[2025,8,2]],"date-time":"2025-08-02T17:24:34Z","timestamp":1754155474000},"page":"1285-1300","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Assessment of the Orbit\u00ae subretinal delivery system (Orbit\u00ae SDS device) and prototypes in adult and juvenile canine eyes"],"prefix":"10.1007","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1995-0095","authenticated-orcid":false,"given":"Jennifer C.","family":"Kwok","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1858-1491","authenticated-orcid":false,"given":"Alexa P.","family":"Gray","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5940-1349","authenticated-orcid":false,"given":"Yu","family":"Sato","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Svetlana","family":"Savina","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia","family":"Dolgova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7839-5178","authenticated-orcid":false,"given":"Raghavi","family":"Sudharsan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8803-9369","authenticated-orcid":false,"given":"Sanford L.","family":"Boye","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7312-3197","authenticated-orcid":false,"given":"Shannon E.","family":"Boye","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sam","family":"Reichenbacker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tom","family":"Meyer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kirsten Stoner","family":"Cummiskey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1127-4774","authenticated-orcid":false,"given":"William A.","family":"Beltran","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,8,2]]},"reference":[{"key":"1929_CR1","doi-asserted-by":"publisher","unstructured":"Tripepi D, Jalil A, Ally N, et al. 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