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The present research work aimed to investigate the modulatory impacts of <jats:italic>in ovo<\/jats:italic> delivery of prebiotic and probiotic on oxidative stress, the intestinal transcriptome, and various plasma metabolites in chickens. Fertilized Ross 308 eggs were administered <jats:italic>in ovo<\/jats:italic> either with galactooligosaccharide (GOS) (3.5\u00a0mg\/egg or <jats:italic>Lactiplantibacillus plantarum<\/jats:italic> (LP) 1\u2009\u00d7\u200910<jats:sup>6<\/jats:sup>\/egg on the 12th day of egg incubation. Three hundred viable Ross 308 broiler hatching eggs in total were randomly assigned to four groups, namely, the negative control not injected group, the group receiving physiological saline injections as the positive control, GOS, and LP. The analysis of genes associated with immune functions, antioxidants, barrier functions, and free fatty acid receptors were determined via qPCR. The analysis of the selected plasma blood metabolites was performed automatically with Pentra C 400. The antioxidant capacity of the chickens\u2019 liver, breast muscle, and spleen was enhanced by the <jats:italic>in ovo<\/jats:italic> injection of GOS and LP. The immune-related gene expression levels were upregulated after <jats:italic>in ovo<\/jats:italic> stimulation with either GOS or LP which improved the gut health of broiler chickens. In addition, several genes related to gut barrier functions were upregulated, thus ensuring epithelial integrity. As for blood plasma metabolites, no adverse effects were observed. In summary, we report that <jats:italic>in ovo<\/jats:italic> stimulation with either GOS or LP stimulates the immune system and improves the antioxidant status and gut health of chickens with no negative impact on plasma blood metabolite indices.<\/jats:p>","DOI":"10.1007\/s13353-024-00931-7","type":"journal-article","created":{"date-parts":[[2024,12,12]],"date-time":"2024-12-12T09:18:50Z","timestamp":1733995130000},"page":"421-434","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Modulatory effects of in ovo delivery of galactooligosaccharide and Lactiplantibacillus plantarum on antioxidant capacity, gene expression, and selected plasma metabolite parameters of broiler chickens"],"prefix":"10.1007","volume":"66","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7601-3444","authenticated-orcid":false,"given":"Modou","family":"Mangan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katarzyna","family":"Po\u0142towicz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cornelia C.","family":"Metges","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Siwek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,12,12]]},"reference":[{"key":"931_CR1","doi-asserted-by":"publisher","first-page":"433","DOI":"10.2478\/aoas-2018-0059","volume":"19","author":"K Abramowicz","year":"2019","unstructured":"Abramowicz K, Krauze M, Ognik K (2019) The effect of a probiotic preparation containing Bacillus subtilis PB6 in the diet of chickens on redox and biochemical parameters in their blood. 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