{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T13:15:52Z","timestamp":1791378952212,"version":"4.3.2"},"reference-count":77,"publisher":"Springer Science and Business Media LLC","issue":"4","license":[{"start":{"date-parts":[[2025,3,18]],"date-time":"2025-03-18T00:00:00Z","timestamp":1742256000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2025,3,18]],"date-time":"2025-03-18T00:00:00Z","timestamp":1742256000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100004281","name":"Narodowe Centrum Nauki","doi-asserted-by":"publisher","award":["SONATA 2015\/19\/D\/NZ1\/03441"],"award-info":[{"award-number":["SONATA 2015\/19\/D\/NZ1\/03441"]}],"id":[{"id":"10.13039\/501100004281","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100015386","name":"Uniwersytet \u015al\u0105ski w Katowicach","doi-asserted-by":"publisher","award":["Research Excellence Initiative"],"award-info":[{"award-number":["Research Excellence Initiative"]}],"id":[{"id":"10.13039\/501100015386","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["J Appl Genetics"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Drought stress can damage crop growth and lead to a decline in yield, thereby affecting food security, especially in regions vulnerable to climate change. SNAC1 (stress-responsive NAC1), the NAC transcription factor family member, plays a crucial role in stomatal movement regulation. Effective regulation of stomatal movement is essential for protecting plants from water loss during adverse conditions. Our hypothesis revolves around altering HvSNAC1 activity by introducing a point mutation in its encoding gene, thereby influencing stomatal dynamics in barley. Two TILLING mutants, each harboring missense mutations in the NAC domain, exhibited higher stomatal density after drought stress compared to the parent cultivar \u2018Sebastian\u2019. These mutants also demonstrated distinct patterns of ABA-induced stomatal movement compared to the wild-type (WT). To delve deeper, we conducted a comprehensive analysis of the transcriptomes of these mutants and the parent cultivar \u2018Sebastian\u2019 under both optimal watering conditions and 10\u00a0days of drought stress treatment. We identified differentially expressed genes (DEGs) between the mutants and WT plants under control and drought conditions. Furthermore, we pinpointed DEGs specifically expressed in both mutants under drought conditions. Our experiments revealed that the\n                    <jats:italic>cis<\/jats:italic>\n                    -regulatory motif CACG, previously identified in Arabidopsis and rice, is recognized by HvSNAC1 in vitro. Enrichment analysis led to the identification of the cell wall organization category and potential target genes, such as\n                    <jats:italic>HvEXPA8<\/jats:italic>\n                    (\n                    <jats:italic>expansin 8<\/jats:italic>\n                    ),\n                    <jats:italic>HvXTH<\/jats:italic>\n                    (\n                    <jats:italic>xyloglucan endotransglucosylase\/hydrolase<\/jats:italic>\n                    ), and\n                    <jats:italic>HvPAE9<\/jats:italic>\n                    (\n                    <jats:italic>pectin acetylesterase 9<\/jats:italic>\n                    ), suggesting their regulation by HvSNAC1. These findings suggest that HvSNAC1 may play a role in regulating genes associated with stomatal density, size and reopening.\n                  <\/jats:p>","DOI":"10.1007\/s13353-025-00956-6","type":"journal-article","created":{"date-parts":[[2025,3,18]],"date-time":"2025-03-18T03:41:31Z","timestamp":1742269291000},"page":"817-840","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Functional analysis of HvSNAC1 in stomatal dynamics and drought adaptation"],"prefix":"10.1007","volume":"66","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9507-6790","authenticated-orcid":false,"given":"Marzena","family":"Kurowska","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agnieszka","family":"Janiak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Krzysztof","family":"Sitko","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Izabela","family":"Potocka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monika","family":"Gajecka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ewa","family":"Sybilska","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomasz","family":"P\u0142ociniczak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabina","family":"Lip","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magdalena","family":"Rynkiewicz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Klaudia","family":"Wiecha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ma\u0142gorzata","family":"Nawrot","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agata","family":"Daszkowska-Golec","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iwona","family":"Szarejko","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,3,18]]},"reference":[{"key":"956_CR1","doi-asserted-by":"publisher","first-page":"401","DOI":"10.1007\/s11032-013-9958-1","volume":"33","author":"AM Al Abdallat","year":"2014","unstructured":"Al Abdallat AM, Ayad JY, Abu Elenein JM, Al Ajlouni Z, Harwood WA (2014) Overexpression of the transcription factor HvSNAC1 improves drought tolerance in barley (Hordeum vulgare L.). Mol Breeding 33:401\u2013414. https:\/\/doi.org\/10.1007\/s11032-013-9958-1","journal-title":"Mol Breeding"},{"key":"956_CR2","doi-asserted-by":"publisher","first-page":"10296","DOI":"10.1073\/pnas.1004788107","volume":"107","author":"AGL Assun\u00e7\u00e3o","year":"2010","unstructured":"Assun\u00e7\u00e3o AGL, Herrero E, Lin Y-F, Huettel B, Talukdar S, Smaczniak C, Immink RGH, Van Eldik M, Fiers M, Schat H, Aarts MGM (2010) Arabidopsis thaliana transcription factors bZIP19 and bZIP23 regulate the adaptation to zinc deficiency. Proc Natl Acad Sci USA 107:10296\u201310301. https:\/\/doi.org\/10.1073\/pnas.1004788107","journal-title":"Proc Natl Acad Sci USA"},{"key":"956_CR3","doi-asserted-by":"publisher","first-page":"3523","DOI":"10.1093\/jxb\/ers100","volume":"63","author":"NJ Atkinson","year":"2012","unstructured":"Atkinson NJ, Urwin PE (2012) The interaction of plant biotic and abiotic stresses: from genes to the field. J Exp Bot 63:3523\u20133543. https:\/\/doi.org\/10.1093\/jxb\/ers100","journal-title":"J Exp Bot"},{"key":"956_CR4","doi-asserted-by":"publisher","first-page":"346","DOI":"10.1093\/mp\/ssq080","volume":"4","author":"S Balazadeh","year":"2011","unstructured":"Balazadeh S, Kwasniewski M, Caldana C, Mehrnia M, Zanor MI, Xue G-P, Mueller-Roeber B (2011) ORS1, an H\u2082O\u2082-responsive NAC transcription factor, controls senescence in Arabidopsis thaliana. Mol Plant 4:346\u2013360. https:\/\/doi.org\/10.1093\/mp\/ssq080","journal-title":"Mol Plant"},{"key":"956_CR5","doi-asserted-by":"publisher","unstructured":"Barrs H, Weatherley P (1962) A re-examination of the relative turgidity technique for estimating water deficits in leaves. Aust Jnl of Bio Sci 15:413. https:\/\/doi.org\/10.1071\/BI9620413","DOI":"10.1071\/BI9620413"},{"key":"956_CR6","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.fcr.2007.07.004","volume":"105","author":"L Cattivelli","year":"2008","unstructured":"Cattivelli L, Rizza F, Badeck F-W, Mazzucotelli E, Mastrangelo AM, Francia E, Mar\u00e8 C, Tondelli A, Stanca AM (2008) Drought tolerance improvement in crop plants: an integrated view from breeding to genomics. Field Crop Res 105:1\u201314. https:\/\/doi.org\/10.1016\/j.fcr.2007.07.004","journal-title":"Field Crop Res"},{"key":"956_CR7","doi-asserted-by":"publisher","first-page":"55","DOI":"10.1007\/s13238-011-1010-9","volume":"2","author":"Q Chen","year":"2011","unstructured":"Chen Q, Wang Q, Xiong L, Lou Z (2011) A structural view of the conserved domain of rice stress-responsive NAC1. Protein Cell 2:55\u201363. https:\/\/doi.org\/10.1007\/s13238-011-1010-9","journal-title":"Protein Cell"},{"key":"956_CR8","doi-asserted-by":"publisher","first-page":"4009","DOI":"10.1093\/jxb\/eru046","volume":"65","author":"MW Christiansen","year":"2014","unstructured":"Christiansen MW, Gregersen PL (2014) Members of the barley NAC transcription factor gene family show differential co-regulation with senescence-associated genes during senescence of flag leaves. J Exp Bot 65:4009\u20134022. https:\/\/doi.org\/10.1093\/jxb\/eru046","journal-title":"J Exp Bot"},{"key":"956_CR9","doi-asserted-by":"publisher","unstructured":"Chua LC, Lau OS (2024) Stomatal development in the changing climate. Development 15;151(20):dev202681. https:\/\/doi.org\/10.1242\/dev.202681","DOI":"10.1242\/dev.202681"},{"key":"956_CR10","doi-asserted-by":"publisher","unstructured":"Collin A, Daszkowska-Golec A, Kurowska M, Szarejko I (2020) Barley ABI5 (Abscisic Acid INSENSITIVE 5) is involved in abscisic acid-dependent drought response. Front Plant Sci 11:1138. https:\/\/doi.org\/10.3389\/fpls.2020.01138","DOI":"10.3389\/fpls.2020.01138"},{"key":"956_CR11","doi-asserted-by":"publisher","first-page":"e01563620","DOI":"10.1371\/journalpone0156362","volume":"11","author":"S Daryanto","year":"2016","unstructured":"Daryanto S, Wang L, Jacinthe P-A (2016) Global synthesis of drought effects on maize and wheat production. PLoS One 11:e01563620. https:\/\/doi.org\/10.1371\/journalpone0156362","journal-title":"PLoS One"},{"key":"956_CR12","doi-asserted-by":"publisher","first-page":"4","DOI":"10.3389\/fpls.2013.00138","volume":"13","author":"A Daszkowska-Golec","year":"2013","unstructured":"Daszkowska-Golec A, Szarejko I (2013) Open or close the gate - stomata action under the control of phytohormones in drought stress conditions Front Plant Sci. Frontiers Plant Sci 13:4\u2013138. https:\/\/doi.org\/10.3389\/fpls.2013.00138","journal-title":"Frontiers Plant Sci"},{"key":"956_CR13","doi-asserted-by":"publisher","first-page":"942","DOI":"10.3389\/fpls201700942","volume":"8","author":"A Daszkowska-Golec","year":"2017","unstructured":"Daszkowska-Golec A, Skubacz A, Marzec M, Slota M, Kurowska M, Gajecka M, Gajewska P, P\u0142ociniczak T, Sitko K, Pacak A, Szweykowska-Kulinska Z, Szarejko I (2017) Mutation in HvCBP20 (Cap Binding Protein 20) adapts barley to drought stress at phenotypic and transcriptomic levels. Front Plant Sci 8:942. https:\/\/doi.org\/10.3389\/fpls201700942","journal-title":"Front Plant Sci"},{"key":"956_CR14","doi-asserted-by":"publisher","first-page":"6341","DOI":"10.3390\/ijms20246341","volume":"20","author":"A Daszkowska-Golec","year":"2019","unstructured":"Daszkowska-Golec A, Collin A, Sitko K, Janiak A, Kalaji HM, Szarejko I (2019) Genetic and physiological dissection of photosynthesis in barley exposed to drought stress. Int J Mol Sci 20:6341. https:\/\/doi.org\/10.3390\/ijms20246341","journal-title":"Int J Mol Sci"},{"key":"956_CR15","doi-asserted-by":"publisher","first-page":"237","DOI":"10.1023\/a:1016028530943","volume":"50","author":"M Duval","year":"2002","unstructured":"Duval M, Hsieh T-F, Kim SY, Thomas TL (2002) Molecular characterization of AtNAM: a member of the Arabidopsis NAC domain superfamily. Plant Mol Biol 50:237\u2013248. https:\/\/doi.org\/10.1023\/a:1016028530943","journal-title":"Plant Mol Biol"},{"key":"956_CR16","doi-asserted-by":"publisher","unstructured":"Duvaud S, Gabella C, Lisacek F, Stockinger H, Ioannidis V, Durinx C (2021) Expasy, the Swiss Bioinformatics Resource Portal, as designed by its users. Nucleic Acids Res 2;49(W1):W216-W227. https:\/\/doi.org\/10.1093\/nar\/gkab225","DOI":"10.1093\/nar\/gkab225"},{"issue":"8","key":"956_CR17","doi-asserted-by":"publisher","first-page":"2628","DOI":"10.1093\/bioinformatics\/btz931","volume":"36","author":"SX Ge","year":"2020","unstructured":"Ge SX, Jung D, Yao R (2020) ShinyGO: a graphical gene-set enrichment tool for animals and plants. Bioinformatics 36(8):2628\u20132629. https:\/\/doi.org\/10.1093\/bioinformatics\/btz931","journal-title":"Bioinformatics"},{"key":"956_CR18","doi-asserted-by":"publisher","first-page":"151","DOI":"10.3389\/fpls201400151","volume":"5","author":"D Golldack","year":"2014","unstructured":"Golldack D, Li C, Mohan H, Probst N (2014) Tolerance to drought and salt stress in plants: unraveling the signaling networks. Front Plant Sci 5:151. https:\/\/doi.org\/10.3389\/fpls201400151","journal-title":"Front Plant Sci"},{"key":"956_CR19","doi-asserted-by":"publisher","unstructured":"Gou JY, Miller LM, Hou G, Yu XH, Chen XY, Liu CJ (2012) Acetylesterase-mediated deacetylation of pectin impairs cell elongation, pollen germination, and plant reproduction. Plant Cell 24(1):50\u201365. https:\/\/doi.org\/10.1105\/tpc.111.092411","DOI":"10.1105\/tpc.111.092411"},{"key":"956_CR20","doi-asserted-by":"publisher","first-page":"537","DOI":"10.1016\/jtplants202301005","volume":"28","author":"H Hirt","year":"2023","unstructured":"Hirt H, Al-Babili S, Almeida-Trapp M, Martin A, Aranda M, Bartels D, Bennett M, Blilou I, Boer D, Boulouis A et al (2023) PlantACT! - how to tackle the climate crisis. Trends Plant Sci 28:537\u2013543. https:\/\/doi.org\/10.1016\/jtplants202301005","journal-title":"Trends Plant Sci"},{"key":"956_CR21","doi-asserted-by":"publisher","unstructured":"Hsieh YF, Suslov D, Espen L, Schiavone M, Rautengarten C, Griess-Osowski A, Voiniciuc C, Poirier Y (2023) Cis-Golgi phosphate transporters harboring an EXS domain are essential for plant growth and development. Plant Physiol 31;192(2):1000\u20131015. https:\/\/doi.org\/10.1093\/plphys\/kiad123","DOI":"10.1093\/plphys\/kiad123"},{"key":"956_CR22","doi-asserted-by":"publisher","first-page":"12987","DOI":"10.1073\/pnas0604882103","volume":"103","author":"H Hu","year":"2006","unstructured":"Hu H, Dai M, Yao J, Xiao B, Li X, Zhang Q, Xiong L (2006) Overexpressing a NAM, ATAF, and CUC (NAC) transcription factor enhances drought resistance and salt tolerance in rice. Proc Natl Acad Sci U S A 103:12987\u201312992. https:\/\/doi.org\/10.1073\/pnas0604882103","journal-title":"Proc Natl Acad Sci U S A"},{"key":"956_CR23","doi-asserted-by":"crossref","unstructured":"Intergovernmental Panel on Climate Change\u00a0(2022)\u00a0Climate change 2022: Impacts, adaptation and vulnerability. Contribution of working group II to the sixth assessment report of the intergovernmental panel on climate change. Cambridge University Press. https:\/\/www.ipcc.ch\/report\/ar6\/wg2\/","DOI":"10.1017\/9781009325844"},{"key":"956_CR24","doi-asserted-by":"publisher","first-page":"145","DOI":"10.1007\/s10265-021-01361-w","volume":"135","author":"K Ishida","year":"2022","unstructured":"Ishida K, Yokoyama R (2022) Reconsidering the function of the xyloglucan endotransglucosylase\/hydrolase family. J Plant Res 135:145\u2013156. https:\/\/doi.org\/10.1007\/s10265-021-01361-w","journal-title":"J Plant Res"},{"key":"956_CR25","doi-asserted-by":"publisher","first-page":"100317","DOI":"10.1016\/jxplc2022100317","volume":"3","author":"C Jiang","year":"2022","unstructured":"Jiang C, Lei M, Guo Y, Gao G, Shi L, Jin Y, Cai Y, Himmelbach A, Zhou S, He Q, Yao X, Kan J, Haberer G, Duan F, Li L, Liu J, Zhang J, Spannagl M, Liu C, Stein N, Feng Z, Mascher M, Yang P (2022) A reference-guided TILLING by amplicon-sequencing platform supports forward and reverse genetics in barley Plant. Communications 3:100317. https:\/\/doi.org\/10.1016\/jxplc2022100317","journal-title":"Communications"},{"key":"956_CR26","doi-asserted-by":"publisher","first-page":"3548","DOI":"10.3390\/plants12203548","volume":"12","author":"K-H Kim","year":"2023","unstructured":"Kim K-H, Lee B-M (2023) Effects of climate change and drought tolerance on maize growth. Plants 12:3548. https:\/\/doi.org\/10.3390\/plants12203548","journal-title":"Plants"},{"key":"956_CR27","doi-asserted-by":"publisher","first-page":"3132","DOI":"10.1105\/tpc106043018","volume":"18","author":"Y-S Kim","year":"2006","unstructured":"Kim Y-S, Kim S-G, Park J-E, Park H-Y, Lim M-H, Chua N-H, Park C-M (2006) A membrane-bound NAC transcription factor regulates cell division in. Arabidopsis Plant Cell 18:3132\u20133144. https:\/\/doi.org\/10.1105\/tpc106043018","journal-title":"Arabidopsis Plant Cell"},{"key":"956_CR28","doi-asserted-by":"publisher","first-page":"11","DOI":"10.1093\/protein\/12111","volume":"12","author":"K Kinoshita","year":"1999","unstructured":"Kinoshita K, Sadanami K, Kidera A, Go N (1999) Structural motif of phosphate-binding site common to various protein superfamilies: all-against-all structural comparison of protein-mononucleotide complexes Protein Eng. Protein Eng, Design Select 12:11\u201314. https:\/\/doi.org\/10.1093\/protein\/12111","journal-title":"Protein Eng, Design Select"},{"key":"956_CR29","doi-asserted-by":"publisher","first-page":"111894","DOI":"10.1016\/jplantsci2023111894","volume":"337","author":"M Kurowska","year":"2023","unstructured":"Kurowska M, Daszkowska-Golec A (2023) Molecular mechanisms of SNAC1 (stress-responsive NAC1) in conferring the abiotic stress tolerance. Plant Sci. 337:111894. https:\/\/doi.org\/10.1016\/jplantsci2023111894","journal-title":"Plant Sci."},{"key":"956_CR30","doi-asserted-by":"publisher","first-page":"371","DOI":"10.1007\/s13353-011-0061-1","volume":"52","author":"M Kurowska","year":"2011","unstructured":"Kurowska M, Daszkowska-Golec A, Gruszka D, Marzec M, Szurman M, Szarejko I, Maluszynski M (2011a) TILLING: a shortcut in functional genomics. J Appl Genet 52:371\u2013390. https:\/\/doi.org\/10.1007\/s13353-011-0061-1","journal-title":"J Appl Genet"},{"key":"956_CR31","unstructured":"Kurowska M, Goraus S, Daszkowska-Golec A, Maluszynski M, Szarejko I (2011b)\u00a0Hordeum vulgare subsp vulgare cultivar Sebastian stress-induced transcription factor SNAC1 gene, NCBI GenBank, acc num: JF796130"},{"key":"956_CR32","doi-asserted-by":"publisher","first-page":"325","DOI":"10.1093\/nar\/301325","volume":"30","author":"M Lescot","year":"2002","unstructured":"Lescot M, D\u00e9hais P, Thijs G, Marchal K, Moreau Y, Van de Peer Y, Rouz\u00e9 P, Rombauts S (2002) PlantCARE, a database of plant cis-acting regulatory elements and a portal to tools for in silico analysis of promoter sequences. Nucleic Acids Res 30:325\u2013327. https:\/\/doi.org\/10.1093\/nar\/301325","journal-title":"Nucleic Acids Res"},{"key":"956_CR33","doi-asserted-by":"publisher","first-page":"982","DOI":"10.3389\/fpls201900982","volume":"10","author":"X Li","year":"2019","unstructured":"Li X, Chang Y, Ma S, Shen J, Hu H, Xiong L (2019) Genome-wide identification of SNAC1-targeted genes involved in drought response in rice. Front Plant Sci 10:982. https:\/\/doi.org\/10.3389\/fpls201900982","journal-title":"Front Plant Sci"},{"key":"956_CR34","doi-asserted-by":"publisher","first-page":"130","DOI":"10.1007\/s00425-021-03770-6","volume":"254","author":"X-D Liang","year":"2021","unstructured":"Liang X-D, Shalapy M, Zhao S-F, Liu J-H, Wang J-Y (2021) A stress-responsive transcription factor PeNAC1 regulating beta-d-glucan biosynthetic genes enhances salt tolerance in oat. Planta 254:130. https:\/\/doi.org\/10.1007\/s00425-021-03770-6","journal-title":"Planta"},{"key":"956_CR35","doi-asserted-by":"publisher","first-page":"e86895","DOI":"10.1371\/journalpone0086895","volume":"9","author":"G Liu","year":"2014","unstructured":"Liu G, Li X, Jin S, Liu X, Zhu L, Nie Y, Zhang X (2014a) Overexpression of rice NAC gene SNAC1 improves drought and salt tolerance by enhancing root development and reducing transpiration rate in transgenic cotton. PLoS One 9:e86895. https:\/\/doi.org\/10.1371\/journalpone0086895","journal-title":"PLoS One"},{"key":"956_CR36","doi-asserted-by":"publisher","first-page":"164","DOI":"10.1105\/tpc113118687","volume":"26","author":"S Liu","year":"2014","unstructured":"Liu S, Wang LS, Mei Wei T, Meng C, Meng W, Xia G (2014b) A wheat SIMILAR TO RCD-ONE gene enhances seedling growth and abiotic stress resistance by modulating redox homeostasis and maintaining genomic integrity. Plant Cell 26:164\u2013180. https:\/\/doi.org\/10.1105\/tpc113118687","journal-title":"Plant Cell"},{"key":"956_CR37","doi-asserted-by":"publisher","unstructured":"Liu Z-J, Shao F-X, Tang G-Y, Shan L, Bi Y-P (2009) Cloning and characterization of a transcription factor ZmNAC1 in maize (Zea mays) Yi Chuan 31:199\u2013205. https:\/\/doi.org\/10.3724\/spj1005200900199","DOI":"10.3724\/spj1005200900199"},{"key":"956_CR38","doi-asserted-by":"publisher","first-page":"1701","DOI":"10.1007\/s00299-012-1284-2","volume":"31","author":"M Lu","year":"2012","unstructured":"Lu M, Ying S, Zhang D-F, Shi Y-S, Song Y-C, Wang T-Y, Li Y (2012) A maize stress-responsive NAC transcription factor, ZmSNAC1, confers enhanced tolerance to dehydration in transgenic. Arabidopsis Plant Cell Rep 31:1701\u20131711. https:\/\/doi.org\/10.1007\/s00299-012-1284-2","journal-title":"Arabidopsis Plant Cell Rep"},{"key":"956_CR39","doi-asserted-by":"publisher","first-page":"443","DOI":"10.1007\/s11240-013-0375-2","volume":"115","author":"M Lu","year":"2013","unstructured":"Lu M, Zhang D-F, Shi Y-S, Song Y-C, Wang T-Y, Li Y (2013) Expression of SbSNAC1, a NAC transcription factor from sorghum, confers drought tolerance to transgenic. Arabidopsis Plant Cell Tiss Organ Cult 115:443\u2013455. https:\/\/doi.org\/10.1007\/s11240-013-0375-2","journal-title":"Arabidopsis Plant Cell Tiss Organ Cult"},{"key":"956_CR40","doi-asserted-by":"publisher","first-page":"1547","DOI":"10.1007\/s00425-013-1867-3","volume":"237","author":"P L\u00fc","year":"2013","unstructured":"L\u00fc P, Kang M, Jiang X, Dai F, Gao J, Zhang C (2013) RhEXPA4, a rose expansin gene, modulates leaf growth and confers drought and salt tolerance to. Arabidopsis Planta 237:1547\u20131559. https:\/\/doi.org\/10.1007\/s00425-013-1867-3","journal-title":"Arabidopsis Planta"},{"issue":"7651","key":"956_CR41","doi-asserted-by":"publisher","first-page":"427","DOI":"10.1038\/nature22043","volume":"544","author":"M Mascher","year":"2017","unstructured":"Mascher M, Gundlach H, Himmelbach A, Beier S, Twardziok SO, Wicker T, Radchuk V, Dockter C, Hedley PE, Russell J, Bayer M et al (2017) A chromosome conformation capture ordered sequence of the barley genome. Nature 544(7651):427\u2013433. https:\/\/doi.org\/10.1038\/nature22043","journal-title":"Nature"},{"key":"956_CR42","doi-asserted-by":"publisher","first-page":"1270180","DOI":"10.3389\/fpls20231270180","volume":"14","author":"H Matkowski","year":"2023","unstructured":"Matkowski H, Daszkowska-Golec A (2023) Update on stomata development and action under abiotic stress. Front Plant Sci 14:1270180. https:\/\/doi.org\/10.3389\/fpls20231270180","journal-title":"Front Plant Sci"},{"key":"956_CR43","doi-asserted-by":"publisher","first-page":"201","DOI":"10.1111\/mpp12173","volume":"16","author":"GRD McGrann","year":"2015","unstructured":"McGrann GRD, Steed A, Burt C, Goddard R, Lachaux C, Bansal A, Corbitt M, Gorniak K, Nicholson P, Brown JKM (2015) Contribution of the drought tolerance-related stress-responsive NAC1 transcription factor to resistance of barley to Ramularia leaf spot. Mol Plant Pathol 16:201\u2013209. https:\/\/doi.org\/10.1111\/mpp12173","journal-title":"Mol Plant Pathol"},{"issue":"11","key":"956_CR44","doi-asserted-by":"publisher","first-page":"e4792","DOI":"10.1002\/pro.4792","volume":"32","author":"EC Meng","year":"2023","unstructured":"Meng EC, Goddard TD, Pettersen EF, Couch GS, Pearson ZJ, Morris JH, Ferrin TE (2023) UCSF ChimeraX: tools for structure building and analysis. Protein Sci 32(11):e4792. https:\/\/doi.org\/10.1002\/pro.4792","journal-title":"Protein Sci"},{"key":"956_CR45","doi-asserted-by":"publisher","first-page":"26","DOI":"10.1016\/s0959-440x(00)00163-9","volume":"11","author":"CW M\u00fcller","year":"2001","unstructured":"M\u00fcller CW (2001) Transcription factors: global and detailed views. Curr Opin Struct Biol 11:26\u201332. https:\/\/doi.org\/10.1016\/s0959-440x(00)00163-9","journal-title":"Curr Opin Struct Biol"},{"key":"956_CR46","doi-asserted-by":"publisher","first-page":"97","DOI":"10.1016\/jbbagrm201110005","volume":"1819","author":"K Nakashima","year":"2012","unstructured":"Nakashima K, Takasaki H, Mizoi J, Shinozaki K, Yamaguchi-Shinozaki K (2012) NAC transcription factors in plant abiotic stress responses. Biochim Biophys Acta 1819:97\u2013103. https:\/\/doi.org\/10.1016\/jbbagrm201110005","journal-title":"Biochim Biophys Acta"},{"key":"956_CR47","doi-asserted-by":"publisher","first-page":"188","DOI":"10.1007\/s13205-021-02744-5","volume":"11","author":"S Negi","year":"2021","unstructured":"Negi S, Tak H, Ganapathi TR (2021) Overexpression of MusaSNAC1 improves shoot proliferation in transgenic banana lines. 3 Biotech 11:188. https:\/\/doi.org\/10.1007\/s13205-021-02744-5","journal-title":"3 Biotech"},{"key":"956_CR48","doi-asserted-by":"publisher","first-page":"79","DOI":"10.1016\/jtplants200412010","volume":"10","author":"AN Olsen","year":"2005","unstructured":"Olsen AN, Ernst HA, Leggio LL, Skriver K (2005) NAC transcription factors: structurally distinct, functionally diverse. Trends Plant Sci 10:79\u201387. https:\/\/doi.org\/10.1016\/jtplants200412010","journal-title":"Trends Plant Sci"},{"key":"956_CR49","doi-asserted-by":"publisher","first-page":"239","DOI":"10.1093\/dnares\/106239","volume":"10","author":"H Ooka","year":"2003","unstructured":"Ooka H, Satoh K, Doi K, Nagata T, Otomo Y, Murakami K, Matsubara K, Osato N, Kawai J, Carninci P, Hayashizaki Y, Suzuki K, Kojima K, Takahara Y, Yamamoto K, Kikuchi S (2003) Comprehensive analysis of NAC family genes in Oryza sativa and Arabidopsis thaliana. DNA Res 10:239\u2013247. https:\/\/doi.org\/10.1093\/dnares\/106239","journal-title":"DNA Res"},{"key":"956_CR50","doi-asserted-by":"publisher","first-page":"86","DOI":"10.3389\/fpls201400086","volume":"5","author":"Y Osakabe","year":"2014","unstructured":"Osakabe Y, Osakabe K, Shinozaki K, Tran L-SP (2014) Response of plants to water stress. Front Plant Sci 5:86. https:\/\/doi.org\/10.3389\/fpls201400086","journal-title":"Front Plant Sci"},{"key":"956_CR51","doi-asserted-by":"publisher","unstructured":"Puranik S, Sahu PP, Mandal SN, B VS, Parida SK, Prasad M (2013) Comprehensive genome-wide survey, genomic constitution and expression profiling of the NAC transcription factor family in foxtail millet (Setaria italica L) PLoS One. 8:e64594https:\/\/doi.org\/10.1371\/journalpone0064594","DOI":"10.1371\/journalpone0064594"},{"key":"956_CR52","doi-asserted-by":"publisher","unstructured":"Qi \u675e\u91d1\u82b3 J, Yu \u90c1\u9732 L, Ding \u4e01\u9759\u4e3d J, Ji \u59ec\u6668\u6668 C, Wang \u6c6a\u793e\u4eae S, Wang \u738b\u521b C, Ding \u4e01\u5e7f\u5927 G, Shi \u77f3\u78ca L, Xu \u5f90\u82b3\u68ee F, Cai \u8521\u7ea2\u6885 H (2023) Transcription factor OsSNAC1 positively regulates nitrate transporter gene expression in rice. Plant Physiol. 192:2923\u20132942. https:\/\/doi.org\/10.1093\/plphys\/kiad290","DOI":"10.1093\/plphys\/kiad290"},{"key":"956_CR53","doi-asserted-by":"publisher","first-page":"e40397","DOI":"10.1371\/journalpone0040397","volume":"7","author":"V Ramegowda","year":"2012","unstructured":"Ramegowda V, Senthil-Kumar M, Nataraja KN, Reddy MK, Mysore KS, Udayakumar M (2012) Expression of a finger millet transcription factor, EcNAC1, in tobacco confers abiotic stress-tolerance. PLoS ONE 7:e40397. https:\/\/doi.org\/10.1371\/journalpone0040397","journal-title":"PLoS ONE"},{"key":"956_CR54","doi-asserted-by":"publisher","first-page":"1723","DOI":"10.1007\/s11738-012-0967-1","volume":"34","author":"M Rapacz","year":"2012","unstructured":"Rapacz M, Stepien A, Skorupa K (2012) Internal standards for quantitative RT-PCR studies of gene expression under drought treatment in barley (Hordeum vulgare L): the effects of developmental stage and leaf age. Acta Physiol Plant 34:1723\u20131733","journal-title":"Acta Physiol Plant"},{"key":"956_CR55","doi-asserted-by":"publisher","unstructured":"Saad ASI, Li X, Li H-P, Huang T, Gao C-S, Guo M-W, Cheng W, Zhao G-Y, Liao Y-C (2013) A rice stress-responsive NAC gene enhances tolerance of transgenic wheat to drought and salt stresses. Plant Sci. 203\u2013204, 33\u201340. https:\/\/doi.org\/10.1016\/jplantsci201212016","DOI":"10.1016\/jplantsci201212016"},{"key":"956_CR56","doi-asserted-by":"publisher","first-page":"99","DOI":"10.1186\/s13007-019-0486-9","volume":"15","author":"M Schreiber","year":"2019","unstructured":"Schreiber M, Barakate A, Uzrek N, Macaulay M, Sourdille A, Morris J, Hedley PE, Ramsay L, Waugh R (2019) A highly mutagenised barley (cv Golden Promise) TILLING population coupled with strategies for screening-by-sequencing. Plant Methods 15:99. https:\/\/doi.org\/10.1186\/s13007-019-0486-9","journal-title":"Plant Methods"},{"key":"956_CR57","doi-asserted-by":"publisher","first-page":"100099","DOI":"10.1016\/jtcsw2023100099","volume":"9","author":"L Shahin","year":"2023","unstructured":"Shahin L, Zhang L, Mohnen D, Urbanowicz BR (2023) Insights into pectin O-acetylation in the plant cell wall: structure, synthesis, and modification. Cell Surf 9:100099. https:\/\/doi.org\/10.1016\/jtcsw2023100099","journal-title":"Cell Surf"},{"key":"956_CR58","doi-asserted-by":"publisher","unstructured":"Sim NL, Kumar P, Hu J, Henikoff S, Schneider G, Ng PC (2012)\u00a0SIFT web server: predicting effects of amino acid substitutions on proteins. Nucleic Acids Res 40:W452\u20137. https:\/\/doi.org\/10.1093\/nar\/gks539","DOI":"10.1093\/nar\/gks539"},{"key":"956_CR59","doi-asserted-by":"publisher","first-page":"772","DOI":"10.1111\/tpj14067","volume":"96","author":"M Stranne","year":"2018","unstructured":"Stranne M, Ren Y, Fimognari L, Birdseye D, Yan J, Bardor M, Mollet J-C, Komatsu T, Kikuchi J, Scheller HV, Sakuragi Y (2018) TBL10 is required for O-acetylation of pectic rhamnogalacturonan-I in Arabidopsis thaliana. Plant J 96:772\u2013785. https:\/\/doi.org\/10.1111\/tpj14067","journal-title":"Plant J"},{"key":"956_CR60","doi-asserted-by":"publisher","first-page":"1160695","DOI":"10.3389\/fpls20231160695","volume":"14","author":"M Szurman-Zubrzycka","year":"2023","unstructured":"Szurman-Zubrzycka M, Kurowska M, Till BJ, Szarejko I (2023) Is it the end of TILLING era in plant science? Front Plant Sci 14:1160695. https:\/\/doi.org\/10.3389\/fpls20231160695","journal-title":"Front Plant Sci"},{"key":"956_CR61","doi-asserted-by":"publisher","unstructured":"Szurman-Zubrzycka ME, Zbieszczyk J, Marzec M, Jelonek J, Chmielewska B, Kurowska MM, Krok M, Daszkowska-Golec A, Guzy-Wrobelska J, Gruszka D, Gajecka M, Gajewska P, Stolarek M, Tylec P, Sega P, Lip S, Kude\u0142ko M, Lorek M, Gorniak-Walas M, Malolepszy A, Podsiadlo N, Szyrajew KP, Keisa A, Mbambo Z, Todorowska E, Gaj M, Nita Z, Orlowska-Job W, Maluszynski M, Szarejko I (2018) HorTILLUS-a rich and renewable source of induced mutations for forward\/reverse genetics and pre-breeding programs in barley. (Hordeum vulgare L) Front Plant Sci. 9:216. https:\/\/doi.org\/10.3389\/fpls201800216","DOI":"10.3389\/fpls201800216"},{"issue":"13","key":"956_CR62","doi-asserted-by":"publisher","first-page":"3808","DOI":"10.1093\/nar\/gkg574","volume":"31","author":"NE Taylor","year":"2003","unstructured":"Taylor NE, Greene EA (2003) PARSESNP: A tool for the analysis of nucleotide polymorphisms. Nucleic Acids Res 31(13):3808\u201311. https:\/\/doi.org\/10.1093\/nar\/gkg574","journal-title":"Nucleic Acids Res"},{"key":"956_CR63","doi-asserted-by":"publisher","first-page":"32","DOI":"10.4161\/gmcr1110569","volume":"1","author":"L-SP Tran","year":"2010","unstructured":"Tran L-SP, Nishiyama R, Yamaguchi-Shinozaki K, Shinozaki K (2010) Potential utilization of NAC transcription factors to enhance abiotic stress tolerance in plants by biotechnological approach. GM Crops. 1:32\u201339. https:\/\/doi.org\/10.4161\/gmcr1110569","journal-title":"GM Crops."},{"issue":"9","key":"956_CR64","doi-asserted-by":"publisher","first-page":"2481","DOI":"10.1105\/tpc.104.022699","volume":"16","author":"LS Tran","year":"2004","unstructured":"Tran LS, Nakashima K, Sakuma Y, Simpson SD, Fujita Y, Maruyama K, Fujita M, Seki M, Shinozaki K, Yamaguchi-Shinozaki K (2004) Isolation and functional analysis of Arabidopsis stress-inducible NAC transcription factors thatbind to a drought-responsive cis-element in the early responsive to dehydration stress 1 promoter. Plant Cell. 16(9):2481\u20132498. https:\/\/doi.org\/10.1105\/tpc.104.022699","journal-title":"Plant Cell."},{"key":"956_CR65","doi-asserted-by":"publisher","first-page":"597","DOI":"10.1111\/j1365-313X200502477x","volume":"43","author":"RAM Vreeburg","year":"2005","unstructured":"Vreeburg RAM, Benschop JJ, Peeters AJM, Colmer TD, Ammerlaan AHM, Staal M, Elzenga TM, Staals RHJ, Darley CP, McQueen-Mason SJ, Voesenek LACJ (2005) Ethylene regulates fast apoplastic acidification and expansin A transcription during submergence-induced petiole elongation in Rumex palustris. Plant J 43:597\u2013610. https:\/\/doi.org\/10.1111\/j1365-313X200502477x","journal-title":"Plant J"},{"key":"956_CR66","doi-asserted-by":"publisher","unstructured":"Wang M, Zhao M, Wang M, Liu S, Tian Y, Moon B, Liang C, Li C, Shi W, Bai M-Y, Liu S, Zhang W, Hwang I, Xia G (2022) TaSRO1 plays a dual role in suppressing TaSIP1 to fine tune mitochondrial retrograde signalling and enhance salinity stress tolerance. New Phytol. 236:495\u2013511https:\/\/doi.org\/10.1111\/nph18340","DOI":"10.1111\/nph18340"},{"key":"956_CR67","doi-asserted-by":"publisher","first-page":"395","DOI":"10.1042\/BJ20111742","volume":"444","author":"DH Welner","year":"2012","unstructured":"Welner DH, Lindemose S, Grossmann JG, M\u00f8llegaard NE, Olsen AN, Helgstrand C, Skriver K, Lo Leggio L (2012) DNA binding by the plant-specific NAC transcription factors in crystal and solution: a firm link to WRKY and GCM transcription factors. Biochem J 444:395\u2013404. https:\/\/doi.org\/10.1042\/BJ20111742","journal-title":"Biochem J"},{"key":"956_CR68","doi-asserted-by":"publisher","unstructured":"Wei PC, Zhang XQ, Zhao P, Wang XC (2011) Regulation of stomatal opening by the guard cell expansin AtEXPA1. Plant Signal Behav 6(5):740\u20132. https:\/\/doi.org\/10.4161\/psb.6.5.15144","DOI":"10.4161\/psb.6.5.15144"},{"key":"956_CR69","doi-asserted-by":"publisher","first-page":"482","DOI":"10.1105\/tpc111090894","volume":"24","author":"A Wu","year":"2012","unstructured":"Wu A, Allu AD, Garapat P, Siddiqui H, Dortay H, Zanor M-I, Asensi-Fabado MA, Munn\u00e9-Bosch S, Antonio C, Tohge T, Fernie AR, Kaufmann K, Xue G-P, Mueller-Roeber B, Balazadeh S (2012) JUNGBRUNNEN1, a reactive oxygen species-responsive NAC transcription factor, regulates longevity in. Arabidopsis Plant Cell 24:482\u2013506. https:\/\/doi.org\/10.1105\/tpc111090894","journal-title":"Arabidopsis Plant Cell"},{"key":"956_CR70","doi-asserted-by":"publisher","first-page":"43","DOI":"10.1071\/FP05161","volume":"33","author":"G-P Xue","year":"2006","unstructured":"Xue G-P, Bower NI, McIntyre CL, Riding GA, Kazan K, Shorter R (2006) TaNAC69 from the NAC superfamily of transcription factors is up-regulated by abiotic stresses in wheat and recognises two consensus DNA-binding sequences. Funct Plant Biol 33:43\u201357. https:\/\/doi.org\/10.1071\/FP05161","journal-title":"Funct Plant Biol"},{"key":"956_CR71","doi-asserted-by":"publisher","first-page":"137893","DOI":"10.1016\/jscitotenv2020137893","volume":"723","author":"M Yang","year":"2020","unstructured":"Yang M, Wang G, Ahmed KF, Adugna B, Eggen M, Atsbeha E, You L, Koo J, Anagnostou E (2020) The role of climate in the trend and variability of Ethiopia\u2019s cereal crop yields. Sci Total Environ 723:137893. https:\/\/doi.org\/10.1016\/jscitotenv2020137893","journal-title":"Sci Total Environ"},{"key":"956_CR72","doi-asserted-by":"publisher","first-page":"569","DOI":"10.1093\/jxb\/ers349","volume":"64","author":"J You","year":"2013","unstructured":"You J, Zong W, Xiaokai Li, Ning J, Hu H, Li Xianghua Xiao J, Xiong L (2013) The SNAC1-targeted gene OsSRO1c modulates stomatal closure and oxidative stress tolerance by regulating hydrogen peroxide in rice. J Exp Bot 64:569\u2013583. https:\/\/doi.org\/10.1093\/jxb\/ers349","journal-title":"J Exp Bot"},{"key":"956_CR73","doi-asserted-by":"publisher","first-page":"693","DOI":"10.1007\/s11103-013-0163-8","volume":"84","author":"J You","year":"2014","unstructured":"You J, Zong W, Du H, Hu H, Xiong L (2014a) A special member of the rice SRO family, OsSRO1c, mediates responses to multiple abiotic stresses through interaction with various transcription factors. Plant Mol Biol 84:693\u2013705. https:\/\/doi.org\/10.1007\/s11103-013-0163-8","journal-title":"Plant Mol Biol"},{"key":"956_CR74","doi-asserted-by":"publisher","first-page":"2100","DOI":"10.1104\/pp114251116","volume":"166","author":"J You","year":"2014","unstructured":"You J, Zong W, Hu H, Li X, Xiao J, Xiong L (2014b) A STRESS-RESPONSIVE NAC1-regulated protein phosphatase gene rice protein phosphatase18 modulates drought and oxidative stress tolerance through abscisic acid-independent reactive oxygen species scavenging in rice. Plant Physiol 166:2100\u20132114. https:\/\/doi.org\/10.1104\/pp114251116","journal-title":"Plant Physiol"},{"key":"956_CR75","doi-asserted-by":"publisher","first-page":"27","DOI":"10.1007\/s00299-010-0937-2","volume":"30","author":"X-Q Zhang","year":"2011","unstructured":"Zhang X-Q, Wei P-C, Xiong Y-M, Yang Y, Chen J, Wang X-C (2011) Overexpression of the Arabidopsis \u03b1-expansin gene AtEXPA1 accelerates stomatal opening by decreasing the volumetric elastic modulus. Plant Cell Rep 30:27\u201336. https:\/\/doi.org\/10.1007\/s00299-010-0937-2","journal-title":"Plant Cell Rep"},{"key":"956_CR76","doi-asserted-by":"publisher","first-page":"9326","DOI":"10.1073\/pnas1701762114","volume":"114","author":"C Zhao","year":"2017","unstructured":"Zhao C, Liu B, Piao S, Wang X, Lobell DB, Huang Y, Huang M, Yao Y, Bassu S, Ciais P, Durand J-L, Elliott J, Ewert F, Janssens IA, Li T, Lin E, Liu Q, Martre P, M\u00fcller C, Peng S, Pe\u00f1uelas J, Ruane AC, Wallach D, Wang T, Wu D, Liu Z, Zhu Y, Zhu Z, Asseng S (2017) Temperature increase reduces global yields of major crops in four independent estimates. Proc Natl Acad Sci USA 114:9326\u20139331. https:\/\/doi.org\/10.1073\/pnas1701762114","journal-title":"Proc Natl Acad Sci USA"},{"key":"956_CR77","doi-asserted-by":"publisher","first-page":"1087","DOI":"10.1093\/mp\/ssq062","volume":"3","author":"R Zhong","year":"2010","unstructured":"Zhong R, Lee C, Ye Z-H (2010) Global analysis of direct targets of secondary wall NAC master switches in. Arabidopsis Mol Plant 3:1087\u20131103. https:\/\/doi.org\/10.1093\/mp\/ssq062","journal-title":"Arabidopsis Mol Plant"}],"container-title":["Journal of Applied Genetics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s13353-025-00956-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s13353-025-00956-6\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s13353-025-00956-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,12]],"date-time":"2025-11-12T00:20:29Z","timestamp":1762906829000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s13353-025-00956-6"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,18]]},"references-count":77,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2025,12]]}},"alternative-id":["956"],"URL":"https:\/\/doi.org\/10.1007\/s13353-025-00956-6","relation":{},"ISSN":["1234-1983","2190-3883"],"issn-type":[{"value":"1234-1983","type":"print"},{"value":"2190-3883","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,3,18]]},"assertion":[{"value":"24 July 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"26 February 2025","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"3 March 2025","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"18 March 2025","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}]}}