{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T03:05:12Z","timestamp":1783393512736,"version":"3.54.6"},"reference-count":21,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2022,1,13]],"date-time":"2022-01-13T00:00:00Z","timestamp":1642032000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2022,1,13]],"date-time":"2022-01-13T00:00:00Z","timestamp":1642032000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"}],"funder":[{"DOI":"10.13039\/501100004809","name":"Financiadora de Estudos e Projetos","doi-asserted-by":"publisher","award":["1387\/10"],"award-info":[{"award-number":["1387\/10"]}],"id":[{"id":"10.13039\/501100004809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2013\/08135-2"],"award-info":[{"award-number":["2013\/08135-2"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["465539\/2014-9"],"award-info":[{"award-number":["465539\/2014-9"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["J. Neurovirol."],"published-print":{"date-parts":[[2022,2]]},"DOI":"10.1007\/s13365-020-00924-2","type":"journal-article","created":{"date-parts":[[2022,1,13]],"date-time":"2022-01-13T15:02:46Z","timestamp":1642086166000},"page":"27-34","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Monitoring of HTLV-1-associated diseases by proviral load quantification using multiplex real-time PCR"],"prefix":"10.1007","volume":"28","author":[{"given":"Evandra Strazza","family":"Rodrigues","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suellen","family":"Salustiano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elaine Vieira","family":"Santos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Svetoslav Nanev","family":"Slavov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Virg\u00ednia","family":"Pican\u00e7o-Castro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliana Matos","family":"Ma\u00e7onetto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tissiana Marques","family":"de Haes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Osvaldo Massaiti","family":"Takayanagui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dimas Tadeu","family":"Covas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1487-0141","authenticated-orcid":false,"given":"Simone","family":"Kashima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,1,13]]},"reference":[{"issue":"1-2","key":"924_CR1","doi-asserted-by":"publisher","first-page":"37","DOI":"10.1016\/S0166-0934(01)00445-1","volume":"102","author":"A Deh\u00e9e","year":"2002","unstructured":"Deh\u00e9e A, C\u00e9saire R, D\u00e9sir\u00e9 N, L\u00e9zin A, Bourdonn\u00e9 O, B\u00e9ra O, Plumelle Y, Smadja D, Nicolas JC (2002) Quantitation of HTLV-I proviral load by a TaqMan real-time PCR assay. J Virol Methods 102(1-2):37\u201351","journal-title":"J Virol Methods"},{"issue":"2","key":"924_CR2","doi-asserted-by":"publisher","first-page":"359","DOI":"10.1089\/AID.2012.0132","volume":"29","author":"MA Demontis","year":"2013","unstructured":"Demontis MA, Hilburn S, Taylor GP (2013) Human T cell lymphotropic virus type 1 viral load variability and long-term trends in asymptomatic carriers and in patients with human T cell lymphotropic virus type 1-related diseases. AIDS Res Hum Retroviruses 29(2):359\u2013364. https:\/\/doi.org\/10.1089\/AID.2012.0132","journal-title":"AIDS Res Hum Retroviruses"},{"key":"924_CR3","doi-asserted-by":"publisher","first-page":"559","DOI":"10.1128\/CMR.13.4.559","volume":"13","author":"EM Elnifro","year":"2000","unstructured":"Elnifro EM, Ashshi AM, Cooper RJ, Klapper PE (2000) Multiplex PCR: optimization and application in diagnostic virology. Clin Microbiol Rev 13:559\u2013570","journal-title":"Clin Microbiol Rev"},{"issue":"4","key":"924_CR4","doi-asserted-by":"publisher","first-page":"664","DOI":"10.1002\/jmv.23227","volume":"84","author":"S Furtado Mdos","year":"2012","unstructured":"Furtado Mdos S, Andrade RG, Romanelli LC, Ribeiro MA, Ribas JG, Torres EB, Barbosa-Stancioli EF, Proietti AB, Martins ML (2012) Monitoring the HTLV-1 proviral load in the peripheral blood of asymptomatic carriers and patients with HTLV-associatedmyelopathy\/tropical spastic paraparesis from a Brazilian cohort: ROC curve analysis to establish the threshold for risk disease. J Med Virol 84(4):664\u2013671. https:\/\/doi.org\/10.1002\/jmv.23227","journal-title":"J Med Virol"},{"issue":"3","key":"924_CR5","doi-asserted-by":"publisher","first-page":"584","DOI":"10.1016\/j.jcv.2013.09.003","volume":"58","author":"MF Grassi","year":"2013","unstructured":"Grassi MF, Olavarria VN, Kruschewsky Rde A, Yamano Y, Jacobson S, Taylor GP, Martin F, Galv\u00e3o-Castro B (2013) Utility of HTLV proviral load quantification in diagnosis of HTLV-1-associated myelopathy requires international standardization. J Clin Virol 58(3):584\u2013586. https:\/\/doi.org\/10.1016\/j.jcv.2013.09.003","journal-title":"J Clin Virol"},{"issue":"5","key":"924_CR6","doi-asserted-by":"publisher","first-page":"e0006967","DOI":"10.1371\/journal.pntd.0006967","volume":"1;13","author":"ME Haziot","year":"2019","unstructured":"Haziot ME, Gascon MR, Assone T, Fonseca LAM, Luiz ODC, Smid J, Paiva AM, Marcusso RMDN, de Oliveira ACP, Casseb J (2019) Detection of clinical and neurological signs in apparently asymptomatic HTLV-1 infected carriers: association with high proviral load. PLoS Negl Trop Dis 1;13(5):e0006967. https:\/\/doi.org\/10.1371\/journal.pntd.0006967","journal-title":"PLoS Negl Trop Dis"},{"issue":"8","key":"924_CR7","doi-asserted-by":"publisher","first-page":"1211","DOI":"10.1182\/blood-2009-12-257410","volume":"26;116","author":"M Iwanaga","year":"2010","unstructured":"Iwanaga M, Watanabe T, Utsunomiya A, Okayama A, Uchimaru K, Koh KR, Ogata M, Kikuchi H, Sagara Y, Uozumi K, Mochizuki M, Tsukasaki K, Saburi Y, Yamamura M, Tanaka J, Moriuchi Y, Hino S, Kamihira S, Yamaguchi K (2010) Joint study on predisposing factors of ATL development investigators. Human T-cell leukemia virus type I (HTLV-1) proviral load and disease progression in asymptomatic HTLV-1 carriers: a nationwide prospective study in Japan. Blood 26;116(8):1211\u20131219. https:\/\/doi.org\/10.1182\/blood-2009-12-257410","journal-title":"Blood"},{"issue":"2","key":"924_CR8","doi-asserted-by":"publisher","first-page":"147","DOI":"10.1016\/0165-5728(93)90004-I","volume":"42","author":"R Kubota","year":"1993","unstructured":"Kubota R, Fujiyoshi T, Izumo S, Yashiki S, Maruyama I, Osame M, Sonoda S (1993) Fluctuation of HTLV-I proviral DNA in peripheral blood mononuclear cells of HTLV-I-associated myelopathy. J Neuroimmunol 42(2):147\u2013154","journal-title":"J Neuroimmunol"},{"issue":"11","key":"924_CR9","doi-asserted-by":"publisher","first-page":"3485","DOI":"10.1128\/JCM.01628-15","volume":"53","author":"M Kuramitsu","year":"2015","unstructured":"Kuramitsu M, Okuma K, Yamochi T, Sato T, Sasaki D, Hasegawa H, Umeki K, Kubota R, Sobata R, Matsumoto C, Kaneko N, Naruse I, Yamagishi M, Nakashima M, Momose H, Araki K, Mizukami T, Mizusawa S, Okada Y, Ochiai M, Utsunomiya A, Koh KR, Ogata M, Nosaka K, Uchimaru K, Iwanaga M, Sagara Y, Yamano Y, Satake M, Okayama A, Mochizuki M, Izumo S, Saito S, Itabashi K, Kamihira S, Yamaguchi K, Watanabe T, Hamaguchi I (2015) Standardization of quantitative PCR for human T-cell leukemia virus type 1 in Japan: a collaborative study. J Clin Microbiol 53(11):3485\u20133491. https:\/\/doi.org\/10.1128\/JCM.01628-15","journal-title":"J Clin Microbiol"},{"key":"924_CR10","doi-asserted-by":"publisher","first-page":"275","DOI":"10.1016\/j.jcv.2004.05.016","volume":"31","author":"TH Lee","year":"2004","unstructured":"Lee TH, Chafets DM, Busch MP, Murphy EL (2004) Quantitation of HTLV-I and II proviral load using real-time quantitative PCR with SYBR green chemistry. J Clin Virol 31:275\u2013282","journal-title":"J Clin Virol"},{"issue":"3","key":"924_CR11","doi-asserted-by":"publisher","first-page":"228","DOI":"10.1080\/13550280152403272","volume":"7","author":"T Matsuzaki","year":"2001","unstructured":"Matsuzaki T, Nakagawa M, Nagai M, Usuku K, Higuchi I, Arimura K, Kubota H, Izumo S, Akiba S, Osame M (2001)HTLV-I proviral load correlates with progression of motor disability in HAM\/TSP: analysis of 239 HAM\/TSP patients including 64 patients followed up for 10 years. J Neurovirol 7(3):228\u2013234","journal-title":"J Neurovirol"},{"issue":"7","key":"924_CR12","doi-asserted-by":"publisher","first-page":"665","DOI":"10.1089\/088922200308891","volume":"1;16","author":"WJ Miley","year":"2000","unstructured":"Miley WJ, Suryanarayana K, Manns A, Kubota R, Jacobson S, Lifson JD, Waters D (2000)Real-time polymerase chain reaction assay for cell-associated HTLV type I DNA viral load. AIDS Res Hum Retroviruses 1;16(7):665\u2013675","journal-title":"AIDS Res Hum Retroviruses"},{"key":"924_CR13","doi-asserted-by":"publisher","first-page":"3682","DOI":"10.1128\/JCM.00781-09","volume":"47","author":"B Moens","year":"2009","unstructured":"Moens B, Lopez G, Adaui V, Gonzalez E, Kerremans L, Clark D, Verdonck K, Gotuzzo E, Vanham G, Cassar O, Gessain A, Vandamme AM, Van Dooren S (2009) Development and validation of a multiplex real-time PCR assay for simultaneous genotyping and human T-lymphotropic virus type 1, 2, and 3 proviral load determination. J Clin Microbiol 47:3682\u20133691","journal-title":"J Clin Microbiol"},{"issue":"4","key":"924_CR14","doi-asserted-by":"publisher","first-page":"302","DOI":"10.1016\/j.jcv.2011.12.033","volume":"53","author":"M Naderi","year":"2012","unstructured":"Naderi M, Paryan M, Azadmanesh K, Rafatpanah H, Rezvan H, Mirab Samiee S (2012) Design and development of a quantitative real time PCR assay for monitoring of HTLV-1 provirus in whole blood. J Clin Virol 53(4):302\u2013307. https:\/\/doi.org\/10.1016\/j.jcv.2011.12.033","journal-title":"J Clin Virol"},{"issue":"2","key":"924_CR15","doi-asserted-by":"publisher","first-page":"228","DOI":"10.1080\/13550280390194028","volume":"9","author":"M Nagai","year":"2003","unstructured":"Nagai M, Osame M (2003) Human T-cell lymphotropic virus type I and neurological diseases. J Neurovirol 9(2):228\u2013325 Review","journal-title":"J Neurovirol"},{"issue":"6","key":"924_CR16","doi-asserted-by":"publisher","first-page":"586","DOI":"10.3109\/13550289809114225","volume":"4","author":"M Nagai","year":"1998","unstructured":"Nagai M, Usuku K, Matsumoto W, Kodama D, Takenouchi N, Moritoyo T, Hashiguchi S, Ichinose M, Bangham CR, Izumo S, Osame M (1998) Analysis of HTLV-I proviral load in 202 HAM\/TSP patients and 243 asymptomatic HTLV-I carriers: high proviral load strongly predisposes to HAM\/TSP. J Neurovirol 4(6):586\u2013593","journal-title":"J Neurovirol"},{"issue":"3","key":"924_CR17","doi-asserted-by":"publisher","first-page":"123","DOI":"10.1590\/S0036-46652012000300002","volume":"54","author":"MT Pinto","year":"2012","unstructured":"Pinto MT, Rodrigues ES, Malta TM, Azevedo R, Takayanagui OM, Valente VB, Ubiali EM, Covas DT, Kashima S (2012) HTLV-1\/2 seroprevalence and coinfection rate in Brazilian first-time blood donors: an 11-year follow-up. Rev Inst Med Trop Sao Paulo 54(3):123\u2013129","journal-title":"Rev Inst Med Trop Sao Paulo"},{"key":"924_CR18","unstructured":"Rocha-Junior MC (2014) Desenvolvimento de uma plataforma molecular in house para o diagnostico confirmat\u00f3rio da infec\u00e7\u00e3o pelo HTLV, tese doutorado (link: https:\/\/www.teses.usp.br\/teses\/disponiveis\/60\/60135\/tde-17042015-143833\/pt-br.php) Faculty of Pharmaceutical Sciences of Ribeir\u00e3o Preto, University of S\u00e3o Paulo (USP), Ribeir\u00e3o Preto, S\u00e3o Paulo, Brazil."},{"issue":"2","key":"924_CR19","doi-asserted-by":"publisher","first-page":"536","DOI":"10.1016\/j.jviromet.2013.07.040","volume":"193","author":"C Rosadas","year":"2013","unstructured":"Rosadas C, Cabral-Castro MJ, Vicente AC, Peralta JM, Puccioni-Sohler M (2013) Validation of a quantitative real-time PCR assay for HTLV-1 proviral load in peripheral blood mononuclear cells. J Virol Methods 193(2):536\u2013541. https:\/\/doi.org\/10.1016\/j.jviromet.2013.07.040","journal-title":"J Virol Methods"},{"issue":"1","key":"924_CR20","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002480","volume":"8","author":"S Tattermusch","year":"2012","unstructured":"Tattermusch S, Skinner JA, Chaussabel D, Banchereau J, Berry MP, McNab FW, O'Garra A, Taylor GP, Bangham CR (2012) Systems biology approaches reveal a specific interferon-inducible signature in HTLV-1 associated myelopathy. PLoS Pathog 8(1):e1002480. https:\/\/doi.org\/10.1371\/journal.ppat.1002480","journal-title":"PLoS Pathog"},{"issue":"1","key":"924_CR21","doi-asserted-by":"publisher","first-page":"38","DOI":"10.1016\/j.jcv.2011.05.022","volume":"52","author":"A Waters","year":"2011","unstructured":"Waters A, Oliveira AL, Coughlan S, de Venecia C, Schor D, Leite AC, Ara\u00fajo AQ, Hall WW (2011) Multiplex real-time PCR for the detection and quantitation of HTLV-1 and HTLV-2 proviral load: addressing the issue of indeterminate HTLV results. J Clin Virol 52(1):38\u201344. https:\/\/doi.org\/10.1016\/j.jcv.2011.05.022","journal-title":"J Clin Virol"}],"container-title":["Journal of NeuroVirology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s13365-020-00924-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s13365-020-00924-2\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s13365-020-00924-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,5,6]],"date-time":"2022-05-06T17:33:23Z","timestamp":1651858403000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s13365-020-00924-2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,1,13]]},"references-count":21,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2022,2]]}},"alternative-id":["924"],"URL":"https:\/\/doi.org\/10.1007\/s13365-020-00924-2","relation":{},"ISSN":["1355-0284","1538-2443"],"issn-type":[{"value":"1355-0284","type":"print"},{"value":"1538-2443","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,1,13]]},"assertion":[{"value":"1 April 2020","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"14 October 2020","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"27 October 2020","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"13 January 2022","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Compliance with ethical standards"}},{"value":"The authors declare that they have no conflict of interest.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}}]}}