{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,9]],"date-time":"2026-05-09T10:39:16Z","timestamp":1778323156829,"version":"3.51.4"},"reference-count":36,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,12,24]],"date-time":"2025-12-24T00:00:00Z","timestamp":1766534400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2025,12,24]],"date-time":"2025-12-24T00:00:00Z","timestamp":1766534400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2017\/08215-7"],"award-info":[{"award-number":["2017\/08215-7"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2017\/17943-6"],"award-info":[{"award-number":["2017\/17943-6"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Instituto Pasteur\/S\u00e3o Paulo\/Brazil","award":["Process No. IP02\/17"],"award-info":[{"award-number":["Process No. IP02\/17"]}]},{"name":"Draper & Dash","award":["JRA20190301"],"award-info":[{"award-number":["JRA20190301"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["J. Neurovirol."],"published-print":{"date-parts":[[2026,2]]},"DOI":"10.1007\/s13365-025-01294-3","type":"journal-article","created":{"date-parts":[[2025,12,24]],"date-time":"2025-12-24T14:33:10Z","timestamp":1766586790000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Proteomic and machine learning signatures of rabies virus infection reveal stage-specific biomarkers"],"prefix":"10.1007","volume":"32","author":[{"given":"Iana Suly Santos","family":"Katz","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elaine Raniero","family":"Fernandes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernanda","family":"Guedes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandriana","family":"Ramos Silva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alfonso Portabales","family":"Souza","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Koushik","family":"Vuttha","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leo Kei","family":"Iwai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,12,24]]},"reference":[{"key":"1294_CR1","doi-asserted-by":"publisher","first-page":"347","DOI":"10.1038\/nature19949","volume":"537","author":"R Aebersold","year":"2016","unstructured":"Aebersold R, Mann M (2016) Mass-spectrometric exploration of proteome structure and function. Nature 537:347\u2013355. https:\/\/doi.org\/10.1038\/nature19949","journal-title":"Nature"},{"key":"1294_CR2","doi-asserted-by":"publisher","first-page":"207","DOI":"10.1007\/s12250-017-3968-9","volume":"32","author":"W Ahmad","year":"2017","unstructured":"Ahmad W, Li Y, Guo Y, Wang X, Duan M, Guan Z, Liu Z, Zhang M (2017) Rabies virus co-localizes with early (Rab5) and late (Rab7) endosomal proteins in neuronal and SH-SY5Y cells. Virol Sin 32:207\u2013215. https:\/\/doi.org\/10.1007\/s12250-017-3968-9","journal-title":"Virol Sin"},{"key":"1294_CR3","doi-asserted-by":"publisher","first-page":"2757","DOI":"10.3390\/v14122757","volume":"14","author":"S Ahmed","year":"2022","unstructured":"Ahmed S, van Zalm P, Rudmann EA, Leone M, Keller K, Branda JA, Steen J, Mukerji SS, Steen H (2022) Using CSF proteomics to investigate herpesvirus infections of the central nervous system. Viruses 14:2757. https:\/\/doi.org\/10.3390\/v14122757","journal-title":"Viruses"},{"key":"1294_CR4","doi-asserted-by":"publisher","first-page":"916","DOI":"10.3390\/v16060916","volume":"16","author":"HN Ashraf","year":"2024","unstructured":"Ashraf HN, Uversky VN (2024) Intrinsic disorder in the host proteins entrapped in rabies virus particles. Viruses 16:916. https:\/\/doi.org\/10.3390\/v16060916","journal-title":"Viruses"},{"key":"1294_CR5","doi-asserted-by":"publisher","first-page":"e02140","DOI":"10.1128\/JVI.02140-16","volume":"91","author":"EL Beatman","year":"2017","unstructured":"Beatman EL, Massey A, Shives KD et al (2017) Alpha-synuclein expression restricts RNA viral infections in the brain. J Virol 91:e02140\u2013e02116. https:\/\/doi.org\/10.1128\/JVI.02140-16","journal-title":"J Virol"},{"key":"1294_CR6","doi-asserted-by":"publisher","first-page":"469","DOI":"10.1038\/s41586-020-2332-7","volume":"583","author":"D Bojkova","year":"2020","unstructured":"Bojkova D, Klann K, Koch B, Widera M, Krause D, Ciesek S, Cinatl J, M\u00fcnch C (2020) Proteomics of SARS-CoV-2-infected host cells reveals therapy targets. Nature 583:469\u2013472. https:\/\/doi.org\/10.1038\/s41586-020-2332-7","journal-title":"Nature"},{"key":"1294_CR7","doi-asserted-by":"publisher","first-page":"bbad382","DOI":"10.1093\/bib\/bbad382","volume":"24","author":"S Budhraja","year":"2023","unstructured":"Budhraja S, Doborjeh M, Singh B, Tan S, Doborjeh Z, Lai E, Merkin A, Lee J, Goh W, Kasabov N (2023) Filter and wrapper stacking ensemble (FWSE): a robust approach for reliable biomarker discovery in high-dimensional omics data. Brief Bioinform 24:bbad382. https:\/\/doi.org\/10.1093\/bib\/bbad382","journal-title":"Brief Bioinform"},{"key":"1294_CR8","doi-asserted-by":"publisher","first-page":"512","DOI":"10.1080\/13550280500384982","volume":"11","author":"MCJ Cheeran","year":"2005","unstructured":"Cheeran MCJ, Hu SX, Sheng WS et al (2005) Differential responses of human brain cells to West nile virus infection. J Neurovirol 11:512\u2013524. https:\/\/doi.org\/10.1080\/13550280500384982","journal-title":"J Neurovirol"},{"key":"1294_CR9","doi-asserted-by":"publisher","first-page":"e0323931","DOI":"10.1371\/journal.pone.0323931","volume":"20","author":"P Chienwichai","year":"2025","unstructured":"Chienwichai P, Ketsuwan K, Lumlertdacha B et al (2025) Proteomic and phosphoproteomic analysis of rabies pathogenesis in the clinical canine brain and identification of a kinase inhibitor as a potential repurposed antiviral agent. PLoS ONE 20:e0323931. https:\/\/doi.org\/10.1371\/journal.pone.0323931","journal-title":"PLoS ONE"},{"key":"1294_CR10","doi-asserted-by":"publisher","first-page":"943","DOI":"10.3390\/v17070943","volume":"17","author":"KE Dooley","year":"2025","unstructured":"Dooley KE, Morimoto M, Kaszuba P et al (2025) Evidence generation for a host-response biosignature of respiratory disease. Viruses 17:943. https:\/\/doi.org\/10.3390\/v17070943","journal-title":"Viruses"},{"key":"1294_CR11","doi-asserted-by":"publisher","first-page":"82","DOI":"10.1186\/s12974-020-01755-8","volume":"17","author":"MI Fonseca","year":"2020","unstructured":"Fonseca MI, Chu SH, Hernandez MX et al (2020) Contribution of complement activation pathways to viral clearance and neuroinflammation in viral encephalitis. J Neuroinflammation 17:82. https:\/\/doi.org\/10.1186\/s12974-020-01755-8","journal-title":"J Neuroinflammation"},{"key":"1294_CR12","doi-asserted-by":"publisher","first-page":"122","DOI":"10.1111\/imr.13120","volume":"309","author":"LR Freeman","year":"2022","unstructured":"Freeman LR, Ting JP (2022) The NLRP3 inflammasome: an overview of mechanisms of activation and regulation during viral infection. Immunol Rev 309:122\u2013132. https:\/\/doi.org\/10.1111\/imr.13120","journal-title":"Immunol Rev"},{"key":"1294_CR13","doi-asserted-by":"publisher","DOI":"10.1021\/acschemneuro.5c00478","author":"JF Gemignani","year":"2025","unstructured":"Gemignani JF, Netz PA, Izecksohn D et al (2025) An amyloidogenic fragment of the Spike protein from SARS-CoV-2 virus stimulates the aggregation and toxicity of parkinson\u2019s disease protein alpha-synuclein. ACS Chem Neurosci. https:\/\/doi.org\/10.1021\/acschemneuro.5c00478","journal-title":"ACS Chem Neurosci"},{"key":"1294_CR14","doi-asserted-by":"publisher","first-page":"e0003709","DOI":"10.1371\/journal.pntd.0003709","volume":"9","author":"K Hampson","year":"2015","unstructured":"Hampson K, Coudeville L, Lembo T et al (2015) Estimating the global burden of endemic canine rabies. PLoS Negl Trop Dis 9:e0003709. https:\/\/doi.org\/10.1371\/journal.pntd.0003709","journal-title":"PLoS Negl Trop Dis"},{"key":"1294_CR15","first-page":"39","volume-title":"Virology methods manual","author":"JC Hierholzer","year":"1996","unstructured":"Hierholzer JC, Killington RA (1996) Suspension assay method. In: Mahy BWJ, Kangro HO (eds) Virology methods manual. Academic, San Diego, pp 39\u201340"},{"key":"1294_CR16","doi-asserted-by":"publisher","first-page":"e70579","DOI":"10.1002\/alz.70579","volume":"21","author":"YN Huang","year":"2025","unstructured":"Huang YN, Liu S, Park T et al (2025) Plasma proteomic analysis identifies proteins and pathways related to alzheimer\u2019s risk. Alzheimers Dement 21:e70579. https:\/\/doi.org\/10.1002\/alz.70579","journal-title":"Alzheimers Dement"},{"key":"1294_CR17","doi-asserted-by":"publisher","first-page":"38","DOI":"10.1007\/s11908-016-0540-y","volume":"18","author":"AC Jackson","year":"2016","unstructured":"Jackson AC (2016) Human rabies: a 2016 update. Curr Infect Dis Rep 18:38. https:\/\/doi.org\/10.1007\/s11908-016-0540-y","journal-title":"Curr Infect Dis Rep"},{"key":"1294_CR18","doi-asserted-by":"publisher","first-page":"4173","DOI":"10.1111\/jcmm.16490","volume":"25","author":"L Jiaqi","year":"2021","unstructured":"Jiaqi L, Shengliang C, Guofei D et al (2021) The role of 14-3-3 proteins in cell signalling pathways and virus infection. J Cell Mol Med 25:4173\u20134182. https:\/\/doi.org\/10.1111\/jcmm.16490","journal-title":"J Cell Mol Med"},{"key":"1294_CR19","doi-asserted-by":"publisher","first-page":"3251","DOI":"10.1007\/s00705-017-3484-0","volume":"162","author":"ISS Katz","year":"2017","unstructured":"Katz ISS, Guedes F, Fernandes ER, Dos Ramos Silva S (2017) Immunological aspects of rabies: a literature review. Arch Virol 162:3251\u20133268. https:\/\/doi.org\/10.1007\/s00705-017-3484-0","journal-title":"Arch Virol"},{"key":"1294_CR20","doi-asserted-by":"publisher","first-page":"147","DOI":"10.1111\/j.1471-4159.2012.07859.x","volume":"123","author":"CM Kopil","year":"2012","unstructured":"Kopil CM, Siebert AP, Foskett JK, Neumar RW (2012) Calpain-cleaved type 1 inositol 1,4,5-trisphosphate receptor impairs ER Ca\u00b2\u207a buffering and causes neurodegeneration in primary cortical neurons. J Neurochem 123:147\u2013158. https:\/\/doi.org\/10.1111\/j.1471-4159.2012.07859.x","journal-title":"J Neurochem"},{"key":"1294_CR21","doi-asserted-by":"publisher","first-page":"e65762","DOI":"10.7554\/eLife.65762","volume":"10","author":"T Lebratti","year":"2021","unstructured":"Lebratti T, Lim YS, Cofie A et al (2021) A sustained type I IFN\u2013neutrophil\u2013IL-18 axis drives pathology during mucosal viral infection. Elife 10:e65762. https:\/\/doi.org\/10.7554\/eLife.65762","journal-title":"Elife"},{"key":"1294_CR22","doi-asserted-by":"publisher","first-page":"21336","DOI":"10.18632\/oncotarget.15184","volume":"8","author":"L Li","year":"2017","unstructured":"Li L, Jin H, Wang H et al (2017) Autophagy is highly targeted among host comparative proteomes during infection with different virulent RABV strains. Oncotarget 8:21336\u201321350. https:\/\/doi.org\/10.18632\/oncotarget.15184","journal-title":"Oncotarget"},{"key":"1294_CR23","doi-asserted-by":"publisher","first-page":"90","DOI":"10.1016\/j.coi.2020.06.002","volume":"66","author":"M Li","year":"2020","unstructured":"Li M, Ramage H, Cherry S (2020) Deciphering flavivirus-host interactions using quantitative proteomics. Curr Opin Immunol 66:90\u201397. https:\/\/doi.org\/10.1016\/j.coi.2020.06.002","journal-title":"Curr Opin Immunol"},{"key":"1294_CR24","doi-asserted-by":"publisher","first-page":"154","DOI":"10.1111\/jnc.13526","volume":"137","author":"X Mao","year":"2016","unstructured":"Mao X, Phanavanh B, Hamdan H, Moerman-Herzog AM, Barger SW (2016) NF\u03baB-inducing kinase inhibits NF\u03baB activity specifically in neurons of the CNS. J Neurochem 137:154\u2013163. https:\/\/doi.org\/10.1111\/jnc.13526","journal-title":"J Neurochem"},{"key":"1294_CR25","doi-asserted-by":"publisher","first-page":"97","DOI":"10.1089\/omi.2015.0137","volume":"20","author":"S Mehta","year":"2015","unstructured":"Mehta S, Sreenivasamurthy S, Banerjee S, Mukherjee S, Prasad K, Chowdhary A (2015) Pathway analysis of proteomics profiles in rabies infection: towards future biomarkers? OMICS 20:97\u2013109. https:\/\/doi.org\/10.1089\/omi.2015.0137","journal-title":"OMICS"},{"key":"1294_CR26","doi-asserted-by":"publisher","first-page":"345","DOI":"10.1038\/nrn3961","volume":"16","author":"FM Menzies","year":"2015","unstructured":"Menzies FM, Fleming A, Rubinsztein DC (2015) Compromised autophagy and neurodegenerative diseases. Nat Rev Neurosci 16:345\u2013357. https:\/\/doi.org\/10.1038\/nrn3961","journal-title":"Nat Rev Neurosci"},{"key":"1294_CR27","doi-asserted-by":"publisher","first-page":"789","DOI":"10.1038\/nrm2789","volume":"10","author":"DK Morrison","year":"2009","unstructured":"Morrison DK (2009) The 14-3-3 proteins: regulators of signaling proteins at multiple levels. Nat Rev Mol Cell Biol 10:789\u2013802. https:\/\/doi.org\/10.1038\/nrm2789","journal-title":"Nat Rev Mol Cell Biol"},{"key":"1294_CR28","doi-asserted-by":"publisher","first-page":"16","DOI":"10.1016\/j.gde.2014.04.003","volume":"26","author":"JL Schneider","year":"2014","unstructured":"Schneider JL, Cuervo AM (2014) Autophagy and human disease: emerging themes. Curr Opin Genet Dev 26:16\u201323. https:\/\/doi.org\/10.1016\/j.gde.2014.04.003","journal-title":"Curr Opin Genet Dev"},{"key":"1294_CR29","doi-asserted-by":"publisher","first-page":"636623","DOI":"10.3389\/fimmu.2021.636623","volume":"12","author":"C Sullivan","year":"2021","unstructured":"Sullivan C, Soos BL, Millard PJ, Kim CH, King BL (2021) Modeling virus-induced inflammation in zebrafish: a balance between infection control and excessive inflammation. Front Immunol 12:636623. https:\/\/doi.org\/10.3389\/fimmu.2021.636623","journal-title":"Front Immunol"},{"key":"1294_CR30","doi-asserted-by":"publisher","first-page":"165","DOI":"10.1016\/B978-0-12-387040-7.00008-1","volume":"79","author":"G Ugolini","year":"2011","unstructured":"Ugolini G (2011) Rabies virus as a transneuronal tracer of neuronal connections. Adv Virus Res 79:165\u2013202. https:\/\/doi.org\/10.1016\/B978-0-12-387040-7.00008-1","journal-title":"Adv Virus Res"},{"key":"1294_CR31","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1186\/1559-0275-10-3","volume":"10","author":"AK Venugopal","year":"2013","unstructured":"Venugopal AK, Ghantasala SS, Selvan LD et al (2013) Quantitative proteomics for identifying biomarkers for rabies. Clin Proteom 10:3. https:\/\/doi.org\/10.1186\/1559-0275-10-3","journal-title":"Clin Proteom"},{"key":"1294_CR32","unstructured":"WHO (2023) Rabies fact sheet. World Health Organization. https:\/\/www.who.int\/news-room\/fact-sheets\/detail\/rabies . Accessed 2025"},{"key":"1294_CR33","doi-asserted-by":"publisher","first-page":"2508","DOI":"10.1056\/NEJMoa050382","volume":"352","author":"RE Willoughby Jr","year":"2005","unstructured":"Willoughby RE Jr, Tieves KS, Hoffman GM et al (2005) Survival after treatment of rabies with induction of coma. N Engl J Med 352:2508\u20132514. https:\/\/doi.org\/10.1056\/NEJMoa050382","journal-title":"N Engl J Med"},{"key":"1294_CR34","doi-asserted-by":"publisher","first-page":"423","DOI":"10.4149\/av_2019_406","volume":"63","author":"W Yan","year":"2019","unstructured":"Yan W, Xiang R, Chen J et al (2019) Proteomics analysis of suckling mouse brain infected with attenuated rabies virus strain SRV9. Acta Virol 63:423\u2013432. https:\/\/doi.org\/10.4149\/av_2019_406","journal-title":"Acta Virol"},{"key":"1294_CR35","first-page":"526","volume":"20","author":"HJ Yuan","year":"2004","unstructured":"Yuan HJ, Hu RL, Bao SJ, Zhang SF (2004) Cloning and immunological characteristics of canine IL-18 cDNA. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 20:526\u2013529","journal-title":"Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi"},{"key":"1294_CR36","doi-asserted-by":"publisher","first-page":"159","DOI":"10.1186\/1743-422X-9-159","volume":"9","author":"P Zhao","year":"2012","unstructured":"Zhao P, Zhao L, Zhang K et al (2012) Infection with street strain rabies virus induces modulation of the MicroRNA profile of the mouse brain. Virol J 9:159. https:\/\/doi.org\/10.1186\/1743-422X-9-159","journal-title":"Virol J"}],"container-title":["Journal of NeuroVirology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s13365-025-01294-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s13365-025-01294-3","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s13365-025-01294-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T14:28:51Z","timestamp":1772202531000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s13365-025-01294-3"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12,24]]},"references-count":36,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,2]]}},"alternative-id":["1294"],"URL":"https:\/\/doi.org\/10.1007\/s13365-025-01294-3","relation":{"has-preprint":[{"id-type":"doi","id":"10.21203\/rs.3.rs-7652543\/v1","asserted-by":"object"}]},"ISSN":["1355-0284","1538-2443"],"issn-type":[{"value":"1355-0284","type":"print"},{"value":"1538-2443","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,12,24]]},"assertion":[{"value":"18 September 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"4 November 2025","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"13 November 2025","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"24 December 2025","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations Declaration of Competing Interest All authors declare that they have no competing interests."}},{"value":"not applicable.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Clinical trial number"}},{"value":"All authors declare that they have no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"2"}}