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Previous studies have indicated that the downregulation of TGFB2-AS1 can enhance the stem-like properties of tumor cells, thereby promoting TNBC progression. Bioinformatics analysis has revealed the regulatory role of GATA6 in TGFB2-AS1 transcription, providing insights into the transcriptional regulation of TGFB2-AS1 by GATA6 and offering potential prognostic biomarkers and therapeutic strategies for TNBC.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Bioinformatics analysis, Western blot, and qPCR were employed to assess the expression of GATA6 and TGFB2-AS1. Immunohistochemistry (IHC) and RNA in situ hybridization (ISH) were performed on clinical samples to evaluate GATA6 and TGFB2-AS1 expression, respectively, with survival analysis based on follow-up data. Fluorescence in situ hybridization (FISH), chromatin immunoprecipitation (ChIP), and dual-luciferase reporter assays were used to elucidate the regulatory mechanisms of GATA6 on TGFB2-AS1. Functional experiments, Western blotting, qPCR, and tail vein metastasis assays, were conducted to investigate the role of GATA6-regulated TGFB2-AS1 in TNBC.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>GATA6 binds to the TGFB2-AS1 promoter and represses its transcription, and patients with tumors exhibiting high GATA6 and low TGFB2-AS1 expression are associated with poor prognosis. Both in vivo and in vitro functional experiments confirmed that TGFB2-AS1 critically mediates the tumor-promoting effects of GATA6 in TNBC progression.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Our findings reveal that GATA6 drives the progression of TNBC by repressing TGFB2-AS1 transcription.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1007\/s13402-026-01195-5","type":"journal-article","created":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T07:13:24Z","timestamp":1775027604000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Transcriptional repression of TGFB2-AS1 by GATA6 drives triple-negative breast cancer metastasis"],"prefix":"10.1007","volume":"49","author":[{"given":"Chang","family":"Liu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qianru","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Difei","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zheng","family":"Duan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiao","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyu","family":"Qi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiayin","family":"Ye","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianrong","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cixiang","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,4,1]]},"reference":[{"key":"1195_CR1","doi-asserted-by":"publisher","first-page":"10","DOI":"10.3322\/caac.21871","volume":"75","author":"RL Siegel","year":"2025","unstructured":"R.L. 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