{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T21:05:14Z","timestamp":1793394314020,"version":"4.5.0"},"reference-count":42,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2012,3,29]],"date-time":"2012-03-29T00:00:00Z","timestamp":1332979200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J cachexia sarcopenia muscle"],"published-print":{"date-parts":[[2012,9]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Burn injury results in a chronic inflammatory, hypermetabolic, and hypercatabolic state persisting long after initial injury and wound healing. Burn survivors experience a profound and prolonged loss of lean body mass, fat mass, and bone mineral density, associated with significant morbidity and reduced quality of life. Understanding the mechanisms responsible is essential for developing therapies. A complete characterization of the pathophysiology of burn cachexia in a reproducible mouse model was lacking.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Young adult (12\u201316\u00a0weeks of age) male C57BL\/6J mice were given full thickness burns using heated brass plates or sham injury. Food and water intake, organ and muscle weights, and muscle fiber diameters were measured. Body composition was determined by Piximus. Plasma analyte levels were determined by bead array assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Survival and weight loss were dependent upon burn size. The body weight nadir in burned mice was 14\u00a0days, at which time we observed reductions in total body mass, lean carcass mass, individual muscle weights, and muscle fiber cross\u2010sectional area. Muscle loss was associated with increased expression of the muscle ubiquitin ligase, MuRF1. Burned mice also exhibited reduced fat mass and bone mineral density, concomitant with increased liver, spleen, and heart mass. Recovery of initial body weight occurred at 35\u00a0days; however, burned mice exhibited hyperphagia and polydipsia out to 80\u00a0days. Burned mice had significant increases in serum cytokine, chemokine, and acute phase proteins, consistent with findings in human burn subjects.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This study describes a mouse model that largely mimics human pathophysiology following severe burn injury. These baseline data provide a framework for mouse\u2010based pharmacological and genetic investigation of burn\u2010injury\u2010associated cachexia.<\/jats:p><\/jats:sec>","DOI":"10.1007\/s13539-012-0062-x","type":"journal-article","created":{"date-parts":[[2012,3,28]],"date-time":"2012-03-28T12:52:05Z","timestamp":1332939125000},"page":"199-211","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":65,"title":["Inflammation, organomegaly, and muscle wasting despite hyperphagia in a mouse model of burn cachexia"],"prefix":"10.1002","volume":"3","author":[{"given":"Felipe E.","family":"Pedroso","sequence":"first","affiliation":[{"name":"Department of Cancer Biology, Kimmel Cancer Center Thomas Jefferson University  233 South 10th. 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Street BLSB 306 19107 Philadelphia PA"},{"name":"DeWitt Daughtry Family Department of Surgery University of Miami Miller School of Medicine  Miami FL"},{"name":"Division of Burns University of Miami Miller School of Medicine  Miami FL"},{"name":"Graduate Program in Cancer Biology, Sylvester Comprehensive Cancer Center University of Miami Miller School of Medicine  Miami FL"},{"name":"Department of Surgery Thomas Jefferson University  Philadelphia PA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,3,29]]},"reference":[{"key":"e_1_2_6_2_1","unstructured":"ABA. Burn incidence and treatment in the US: 2011 fact sheet. Chicago: American Burn Association; 2011. Accessed 23 Aug 2011."},{"key":"e_1_2_6_3_1","unstructured":"ABA. American Burn Association National Burn Repository. 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