{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T20:37:54Z","timestamp":1778877474592,"version":"3.51.4"},"reference-count":49,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2013,11,13]],"date-time":"2013-11-13T00:00:00Z","timestamp":1384300800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J cachexia sarcopenia muscle"],"published-print":{"date-parts":[[2014,6]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Appetite is frequently affected in cancer patients leading to anorexia and consequently insufficient food intake. In this study, we report on hypothalamic gene expression profile of a cancer\u2010cachectic mouse model with increased food intake. In this model, mice bearing C26 tumour have an increased food intake subsequently to the loss of body weight. We hypothesise that in this model, appetite\u2010regulating systems in the hypothalamus, which apparently fail in anorexia, are still able to adapt adequately to changes in energy balance. Therefore, studying changes that occur on appetite regulators in the hypothalamus might reveal targets for treatment of cancer\u2010induced eating disorders. By applying transcriptomics, many appetite\u2010regulating systems in the hypothalamus could be taken into account, providing an overview of changes that occur in the hypothalamus during tumour growth.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>C26\u2010colon adenocarcinoma cells were subcutaneously inoculated in 6\u00a0weeks old male CDF1 mice. Body weight and food intake were measured three times a week. On day 20, hypothalamus was dissected and used for transcriptomics using Affymetrix chips.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Food intake increased significantly in cachectic tumour\u2010bearing mice (TB), synchronously to the loss of body weight. Hypothalamic gene expression of orexigenic neuropeptides NPY and AgRP was higher, whereas expression of anorexigenic genes CCK and POMC were lower in TB compared to controls.<\/jats:p><jats:p>In addition, serotonin and dopamine signalling pathways were found to be significantly altered in TB mice. Serotonin levels in brain showed to be lower in TB mice compared to control mice, while dopamine levels did not change. Moreover, serotonin levels inversely correlated with food intake.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Transcriptomic analysis of the hypothalamus of cachectic TB mice with an increased food intake showed changes in NPY, AgRP and serotonin signalling. Serotonin levels in the brain showed to correlate with changes in food intake. Further research has to reveal whether targeting these systems will be a good strategy to avoid the development of cancer\u2010induced eating disorders.<\/jats:p><\/jats:sec><jats:sec><jats:title>Electronic supplementary material<\/jats:title><jats:p>The online version of this article (doi:10.1007\/s13539\u2010013\u20100121\u2010y) contains supplementary material.<\/jats:p><\/jats:sec>","DOI":"10.1007\/s13539-013-0121-y","type":"journal-article","created":{"date-parts":[[2013,11,12]],"date-time":"2013-11-12T15:38:52Z","timestamp":1384270732000},"page":"159-169","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Hypothalamic food intake regulation in a cancer\u2010cachectic mouse model"],"prefix":"10.1002","volume":"5","author":[{"given":"Jvalini T.","family":"Dwarkasing","sequence":"first","affiliation":[{"name":"Nutrition and Pharmacology Group, Division of Human Nutrition Wageningen University  Bomenweg 2 6703 Wageningen"},{"name":"Nutricia Advanced Medical Nutrition Nutricia Research for Specialised Nutrition  Uppsalalaan 12 3584 Utrecht"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miriam","family":"van Dijk","sequence":"additional","affiliation":[{"name":"Nutricia Advanced Medical Nutrition Nutricia Research for Specialised Nutrition  Uppsalalaan 12 3584 Utrecht"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francina J.","family":"Dijk","sequence":"additional","affiliation":[{"name":"Nutricia Advanced Medical Nutrition Nutricia Research for Specialised Nutrition  Uppsalalaan 12 3584 Utrecht"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark V.","family":"Boekschoten","sequence":"additional","affiliation":[{"name":"Nutrition, Metabolism and Genomics Group, Division of Human Nutrition Wageningen University  Bomenweg 2 6703 Wageningen"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joyce","family":"Faber","sequence":"additional","affiliation":[{"name":"Nutricia Advanced Medical Nutrition Nutricia Research for Specialised Nutrition  Uppsalalaan 12 3584 Utrecht"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Josep M.","family":"Argil\u00e8s","sequence":"additional","affiliation":[{"name":"Cancer Research Group, Departament de Bioqu\u00edmica i Biologia Molecular University of Barcelona  Diagonal, 645 08028 Barcelona"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alessandro","family":"Laviano","sequence":"additional","affiliation":[{"name":"Department of Clinical Medicine Sapienza University  Viale dell'Universita' 37 00185 Rome"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"M\u00fcller","sequence":"additional","affiliation":[{"name":"Nutrition, Metabolism and Genomics Group, Division of Human Nutrition Wageningen University  Bomenweg 2 6703 Wageningen"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Renger F.","family":"Witkamp","sequence":"additional","affiliation":[{"name":"Nutrition and Pharmacology Group, Division of Human Nutrition Wageningen University  Bomenweg 2 6703 Wageningen"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Klaske","family":"van Norren","sequence":"additional","affiliation":[{"name":"Nutrition and Pharmacology Group, Division of Human Nutrition Wageningen University  Bomenweg 2 6703 Wageningen"},{"name":"Nutricia Advanced Medical Nutrition Nutricia Research for Specialised Nutrition  Uppsalalaan 12 3584 Utrecht"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,11,13]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.2147\/CMAR.S7101"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(10)70218-7"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/381415a0"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.22.14.5027-5035.2002"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.23.22.7973-7981.2003"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nut.2008.06.005"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjc.6604905"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-4347(93)80062-9"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1515\/jib-2011-160"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gni179"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gng015"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/19.2.185"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-7-538"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.4161\/cc.8.15.9224"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.18632\/aging.100069"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2199-9-5"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1152\/physiolgenomics.00005.2005"},{"key":"e_1_2_6_19_1","first-page":"2290","article-title":"Experimental cancer cachexia induced by transplantable colon 26 adenocarcinoma in mice","volume":"50","author":"Tanaka Y","year":"1990","journal-title":"Cancer Res"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.10338"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910620317"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjc.6690123"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1242\/dmm.008839"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2407-10-363"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00052417"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjc.6604785"},{"key":"e_1_2_6_27_1","first-page":"R1394","article-title":"Anorexia induced by cytokine interactions at pathophysiological concentrations","volume":"270","author":"Sonti G","year":"1996","journal-title":"Am J Physiol"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/syn.10266"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1097540"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.peptides.2004.01.012"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopsych.2012.09.027"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/0361-9230(95)92128-3"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.2001.281.6.R1907"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1191\/0269216302pm612xx"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2008.164152"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/oby.2012.75"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1550-8528.1995.tb00215.x"},{"key":"e_1_2_6_38_1","first-page":"143","article-title":"Elevation of serum free tryptophan, but not brain tryptophan, by serum nonesterified fatty acids","volume":"11","author":"Madras BK","year":"1974","journal-title":"Adv Biochem Psychopharmacol"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbb.2007.10.019"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1054\/clnu.2002.0609"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.jrn.2008.11.008"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1159\/000103323"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2009.02.002"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1359-6446(03)02826-5"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.24784"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.peptides.2006.11.021"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.peptides.2006.10.018"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1080\/01635581.2001.9680621"},{"key":"e_1_2_6_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(96)00329-0"},{"key":"e_1_2_6_50_1","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.31.2250"}],"container-title":["Journal of Cachexia, Sarcopenia and Muscle"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1007%2Fs13539-013-0121-y","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1007\/s13539-013-0121-y","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,28]],"date-time":"2023-10-28T02:45:09Z","timestamp":1698461109000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1007\/s13539-013-0121-y"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,11,13]]},"references-count":49,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2014,6]]}},"alternative-id":["10.1007\/s13539-013-0121-y"],"URL":"https:\/\/doi.org\/10.1007\/s13539-013-0121-y","archive":["Portico"],"relation":{},"ISSN":["2190-5991","2190-6009"],"issn-type":[{"value":"2190-5991","type":"print"},{"value":"2190-6009","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,11,13]]},"assertion":[{"value":"2013-05-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-09-03","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-11-13","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}