{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T16:04:33Z","timestamp":1786982673839,"version":"build-2736575974"},"reference-count":50,"publisher":"Springer Science and Business Media LLC","issue":"6","license":[{"start":{"date-parts":[[2021,10,14]],"date-time":"2021-10-14T00:00:00Z","timestamp":1634169600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0"},{"start":{"date-parts":[[2021,10,14]],"date-time":"2021-10-14T00:00:00Z","timestamp":1634169600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Dermatol Ther (Heidelb)"],"published-print":{"date-parts":[[2021,12]]},"DOI":"10.1007\/s13555-021-00603-y","type":"journal-article","created":{"date-parts":[[2021,10,14]],"date-time":"2021-10-14T01:02:47Z","timestamp":1634173367000},"page":"2043-2056","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Modulation of Photoaging-Induced Cutaneous Elastin: Evaluation of Gene and Protein Expression of Markers Related to Elastogenesis Under Different Photoexposure Conditions"],"prefix":"10.1007","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8963-4522","authenticated-orcid":false,"given":"Ana Cristina","family":"Weihermann","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camila Miranda","family":"de Carvalho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Desir\u00e9e Cigaran","family":"Schuck","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruna Bastos","family":"Swinka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodrigo Makowiecky","family":"Stuart","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruth Maria","family":"Graf","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00e1rcio","family":"Lorencini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carla Abdo","family":"Brohem","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,10,14]]},"reference":[{"issue":"4","key":"603_CR1","doi-asserted-by":"publisher","first-page":"265","DOI":"10.4236\/aar.2014.34036","volume":"3","author":"A Gragnani","year":"2014","unstructured":"Gragnani A, Cornick SM, Chominski V, et\u00a0al. Review of major theories of skin aging. Adv Aging Res. 2014;3(4):265\u201384.","journal-title":"Adv Aging Res"},{"key":"603_CR2","doi-asserted-by":"publisher","first-page":"236","DOI":"10.4161\/derm.23652","volume":"4","author":"F Debacq-Chainiaux","year":"2012","unstructured":"Debacq-Chainiaux F, Leduc C, Verbeke A, Toussaint O. UV, stress and aging. Dermatoendocrinol. 2012;4:236\u201340.","journal-title":"Dermatoendocrinol."},{"key":"603_CR3","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/860479","volume":"2014","author":"A Godic","year":"2014","unstructured":"Godic A, Polj\u0161ak B, Adamic M, et\u00a0al. The role of antioxidants in skin cancer prevention and treatment. Oxid Med Cell Longev. 2014;2014: 860479. https:\/\/doi.org\/10.1155\/2014\/860479.","journal-title":"Oxid Med Cell Longev"},{"issue":"3","key":"603_CR4","doi-asserted-by":"publisher","first-page":"270","DOI":"10.1111\/srt.12643","volume":"25","author":"A Villaret","year":"2019","unstructured":"Villaret A, Ipinazar C, Satar T, et\u00a0al. Raman characterization of human skin aging. Skin Res Technol. 2019;25(3):270\u20136. https:\/\/doi.org\/10.1111\/srt.12643.","journal-title":"Skin Res Technol"},{"key":"603_CR5","doi-asserted-by":"publisher","first-page":"101","DOI":"10.1016\/j.redox.2015.04.003","volume":"5","author":"S Hibbert","year":"2015","unstructured":"Hibbert S, Watson REB, Gibbs NK, et\u00a0al. A potential role for endogenous proteins as sacrificial sunscreens and antioxidants in human tissues. Redox Biol. 2015;5:101\u201313.","journal-title":"Redox Biol"},{"issue":"12","key":"603_CR6","doi-asserted-by":"publisher","first-page":"2900","DOI":"10.1002\/ijc.29335","volume":"136","author":"M Kvaskoff","year":"2015","unstructured":"Kvaskoff M, Pandeya N, Green AC, et\u00a0al. Solar elastosis and cutaneous melanoma: a site-specific analysis. Int J Cancer. 2015;136(12):2900\u201311.","journal-title":"Int J Cancer"},{"issue":"3","key":"603_CR7","doi-asserted-by":"publisher","first-page":"249","DOI":"10.1016\/j.maturitas.2011.04.011","volume":"69","author":"EC Naylor","year":"2011","unstructured":"Naylor EC, Watson REB, Sherratt MJ. Molecular aspects of skin ageing. Maturitas. 2011;69(3):249\u201356.","journal-title":"Maturitas"},{"key":"603_CR8","doi-asserted-by":"publisher","first-page":"163","DOI":"10.1016\/j.biochi.2016.08.010","volume":"128\u2013129","author":"AC Mora Huertas","year":"2016","unstructured":"Mora Huertas AC, Schmeizer CEH, Hoehenwarter W, et\u00a0al. Molecular-level insights into aging processes of skin elastin. Biochimie. 2016;128\u2013129:163\u201373.","journal-title":"Biochimie"},{"key":"603_CR9","doi-asserted-by":"publisher","first-page":"1672","DOI":"10.1021\/ol500333t","volume":"16","author":"T Usuki","year":"2014","unstructured":"Usuki T, Sugimura T, Komatsu A, Koseki Y. Biomimetic chichibabin pyridine synthesis of the COPD biomarkers and elastin cross-linkers isodesmosine and desmosine. Org Lett. 2014;16:1672\u20135.","journal-title":"Org Lett"},{"issue":"6","key":"603_CR10","doi-asserted-by":"publisher","first-page":"1198","DOI":"10.1080\/09168451.2017.1290518","volume":"81","author":"E Uehara","year":"2017","unstructured":"Uehara E, Hokazono H, Hida M, et\u00a0al. GABA promotes elastin synthesis and elastin fiber formation in normal human dermal fibroblasts (HDFs). Biosci Biotechnol Biochem. 2017;81(6):1198\u2013205.","journal-title":"Biosci Biotechnol Biochem"},{"issue":"28","key":"603_CR11","doi-asserted-by":"publisher","first-page":"29185","DOI":"10.1074\/jbc.M313672200","volume":"279","author":"E Hanssen","year":"2004","unstructured":"Hanssen E, Hew FH, Moore E, et\u00a0al. MAGP-2 has multiple binding regions on fibrillins and has covalent periodic association with fibrillin-containing microfibrils. J Biol Chem. 2004;279(28):29185\u201394.","journal-title":"J Biol Chem"},{"issue":"14","key":"603_CR12","doi-asserted-by":"publisher","first-page":"2817","DOI":"10.1242\/jcs.115.14.2817","volume":"115","author":"CM Kielty","year":"2002","unstructured":"Kielty CM, Sherratt MJ, Shuttleworth AC. Elastic fibres. J Cell Sci. 2002;115(14):2817\u201328.","journal-title":"J Cell Sci"},{"issue":"11","key":"603_CR13","doi-asserted-by":"publisher","first-page":"2832","DOI":"10.1021\/acsbiomaterials.6b00564","volume":"3","author":"GC Yeo","year":"2017","unstructured":"Yeo GC, Baldock C, Wise SG, et\u00a0al. Targeted modulation of tropoelastin structure and assembly. ACS Biomater Sci Eng. 2017;3(11):2832\u201344. https:\/\/doi.org\/10.1021\/acsbiomaterials.6b00564.","journal-title":"ACS Biomater Sci Eng"},{"key":"603_CR14","doi-asserted-by":"publisher","first-page":"378","DOI":"10.1111\/j.1600-0625.2008.00799.x","volume":"18","author":"Z Chen","year":"2009","unstructured":"Chen Z, Shin MH, Moon YJ, et\u00a0al. Modulation of elastin exon 26A mRNA and protein expression in human skin in vivo. Exp Dermatol. 2009;18:378\u201386.","journal-title":"Exp Dermatol"},{"issue":"3","key":"603_CR15","doi-asserted-by":"publisher","first-page":"241","DOI":"10.1111\/ics.12372","volume":"39","author":"AC Weihermann","year":"2017","unstructured":"Weihermann AC, Lorencini M, Brohem CA, et\u00a0al. Elastin structure and its involvement in skin photoageing. Int J Cosmet Sci. 2017;39(3):241\u20137. https:\/\/doi.org\/10.1111\/ics.12372.","journal-title":"Int J Cosmet Sci"},{"issue":"5","key":"603_CR16","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1002\/bip.23007","volume":"107","author":"M Miao","year":"2017","unstructured":"Miao M, Reichheld SE, Muiznieks LD, et\u00a0al. Single nucleotide polymorphisms and domain\/splice variants modulate assembly and elastomeric properties of human elastin. Implications for tissue specificity and durability of elastic tissue. Biopolymers. 2017;107(5):1\u201320.","journal-title":"Biopolymers"},{"key":"603_CR17","first-page":"54","volume-title":"Soft tissue augmentation","author":"J Carruthers","year":"2013","unstructured":"Carruthers J, Carruthers A, Alam M. Soft tissue augmentation. 3rd ed. Edinburgh: Elsevier; 2013. p. 54.","edition":"3"},{"issue":"31","key":"603_CR18","doi-asserted-by":"publisher","first-page":"11128","DOI":"10.1021\/bi9802566","volume":"37","author":"F Bisaccia","year":"1998","unstructured":"Bisaccia F, Castiglion-Morelli MA, Spisani S, et\u00a0al. The amino-acid-sequence coded by the rarely expressed exon 26A of human elastin contains a stable beta-turn with chemotactic activity for monocytes. Biochemistry. 1998;37(31):11128\u201335.","journal-title":"Biochemistry"},{"issue":"2","key":"603_CR19","doi-asserted-by":"publisher","first-page":"130","DOI":"10.1016\/S1357-2725(01)00115-7","volume":"34","author":"A Ostuni","year":"2002","unstructured":"Ostuni A, Lograno MD, Gasbarro AR, et\u00a0al. Novel properties of peptides derived from the sequence coded by exon 26A of human elastin. Int J Biochem Cell Biol. 2002;34(2):130\u20135.","journal-title":"Int J Biochem Cell Biol"},{"key":"603_CR20","doi-asserted-by":"publisher","first-page":"1218","DOI":"10.1046\/j.1432-1327.1998.2580001.x","volume":"258","author":"B Vrhovski","year":"1998","unstructured":"Vrhovski B, Weiss AS. Review: biochemistry of tropoelastin. Eur J Biochem. 1998;258:1218.","journal-title":"Eur J Biochem"},{"issue":"7","key":"603_CR21","doi-asserted-by":"publisher","first-page":"1063","DOI":"10.1089\/ars.2013.5653","volume":"21","author":"REB Watson","year":"2014","unstructured":"Watson REB, Gibbs NK, Griffiths CEM, Sherratt MJ. Damage to skin extracellular matrix induced by UV exposure. Antioxid Redox Signal. 2014;21(7):1063\u201377. https:\/\/doi.org\/10.1089\/ars.2013.5653.","journal-title":"Antioxid Redox Signal"},{"key":"603_CR22","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.arr.2014.03.006","volume":"16","author":"MY Akhalaya","year":"2014","unstructured":"Akhalaya MY, Maksimova GV, Rubina AB, et\u00a0al. Molecular action mechanisms of solar infrared radiation and heat on human skin. Ageing Res Rev. 2014;16:1\u201311.","journal-title":"Ageing Res Rev"},{"key":"603_CR23","doi-asserted-by":"publisher","first-page":"6202","DOI":"10.3390\/molecules19056202","volume":"19","author":"A Amaro-Ortiz","year":"2014","unstructured":"Amaro-Ortiz A, Yan B, Dorazio JA. Ultraviolet radiation, aging and the skin: prevention of damage by topical cAMP manipulation. Molecules. 2014;19:6202\u201319.","journal-title":"Molecules"},{"key":"603_CR24","doi-asserted-by":"publisher","first-page":"915","DOI":"10.1046\/j.1523-1747.2001.01358.x","volume":"116","author":"JY Seo","year":"2001","unstructured":"Seo JY, Lee SH, Youn CS, et\u00a0al. Ultraviolet radiation increases tropoelastin mRNA expression in the epidermis of human skin in vivo. J Invest Dermatol. 2001;116:915\u20139.","journal-title":"J Invest Dermatol"},{"issue":"2","key":"603_CR25","doi-asserted-by":"publisher","first-page":"182","DOI":"10.1111\/1523-1747.ep12392693","volume":"103","author":"EF Bernstein","year":"1994","unstructured":"Bernstein EF, Chen YQ, Tamai K, et\u00a0al. Enhanced elastin and fibrillin gene expression in chronically photodamaged skin. J Invest Dermatol. 1994;103(2):182\u20136.","journal-title":"J Invest Dermatol"},{"issue":"9","key":"603_CR26","doi-asserted-by":"publisher","first-page":"735","DOI":"10.1002\/jbio.201300171","volume":"7","author":"ME Darvin","year":"2014","unstructured":"Darvin ME, Richter H, Ahlberg S, et\u00a0al. Influence of sun exposure on the cutaneous collagen\/elastin fibers and carotenoids: negative effects can be reduced by application of sunscreen. J Biophoton. 2014;7(9):735\u201343. https:\/\/doi.org\/10.1002\/jbio.201300171.","journal-title":"J Biophoton"},{"key":"603_CR27","doi-asserted-by":"publisher","first-page":"430","DOI":"10.1007\/s004030100247","volume":"293","author":"E Hirano","year":"2001","unstructured":"Hirano E, Okamoto K, Matsubara MT, et\u00a0al. Elastin expression in cultured human keratinocytes: exon 26A of elastin primary transcript is always included in terminally differentiated keratinocytes. Arch Dermatol Re. 2001;293:430\u20133.","journal-title":"Arch Dermatol Re"},{"key":"603_CR28","doi-asserted-by":"publisher","first-page":"641","DOI":"10.1111\/1523-1747.ep12337639","volume":"109","author":"H Kajiya","year":"1997","unstructured":"Kajiya H, Tanaka N, Inazumi T, et\u00a0al. Cultured human keratinocytes express tropoelastin. J Invest Dermatol. 1997;109:641\u20134.","journal-title":"J Invest Dermatol"},{"issue":"7","key":"603_CR29","doi-asserted-by":"publisher","first-page":"1862","DOI":"10.1038\/jid.2014.90","volume":"134","author":"VF Achterberg","year":"2014","unstructured":"Achterberg VF, Buscemi L, Diekmann H, et\u00a0al. The nano-scale mechanical properties of the extracellular matrix regulate dermal fibroblast function. J Invest Dermatol. 2014;134(7):1862\u201372. https:\/\/doi.org\/10.1038\/jid.2014.90.","journal-title":"J Invest Dermatol"},{"issue":"3","key":"603_CR30","first-page":"270","volume":"58","author":"MJ Fazio","year":"1988","unstructured":"Fazio MJ, Olsen DR, Kuivaniemi H, et\u00a0al. Isolation and characterization of human elastic DNAs, and age-associated variation in elastin gene expression in cultured skin fibroblasts. Lab Invest. 1988;58(3):270\u20137.","journal-title":"Lab Invest"},{"issue":"1","key":"603_CR31","doi-asserted-by":"publisher","first-page":"295","DOI":"10.1002\/term.1913","volume":"11","author":"MJ Merrilees","year":"2017","unstructured":"Merrilees MJ, Falk BA, Zuo N, et\u00a0al. Use of versican variant V3 and versican antisense expression to engineer cultured human skin containing increased content of insoluble elastin. J Tissue Eng Regen Med. 2017;11(1):295\u2013305. https:\/\/doi.org\/10.1002\/term.1913.","journal-title":"J Tissue Eng Regen Med"},{"key":"603_CR32","doi-asserted-by":"publisher","first-page":"507","DOI":"10.1046\/j.1523-1747.2002.01844.x","volume":"119","author":"JH Chung","year":"2002","unstructured":"Chung JH, Seo JY, Lee MK, et\u00a0al. Ultraviolet modulation of human macrophage metalloelastase in human skin in vivo. J Invest Dermatol. 2002;119:507\u201312.","journal-title":"J Invest Dermatol"},{"key":"603_CR33","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/930164","volume":"2013","author":"R Pandel","year":"2013","unstructured":"Pandel R, Poljsak B, Godic A, Dahmane R. Skin photoaging and the role of antioxidants in its prevention. ISRN Dermatol. 2013;2013: 930164. https:\/\/doi.org\/10.1155\/2013\/930164.","journal-title":"ISRN Dermatol"},{"key":"603_CR34","doi-asserted-by":"publisher","first-page":"2598","DOI":"10.1038\/jid.2014.173","volume":"134","author":"A Tewari","year":"2014","unstructured":"Tewari A, Grys K, Kollet J, et\u00a0al. Upregulation of MMP12 and its activity by UVA1 in human skin: potential implications for photoaging. J Invest Dermatol. 2014;134:2598\u2013609.","journal-title":"J Invest Dermatol"},{"key":"603_CR35","doi-asserted-by":"publisher","first-page":"70","DOI":"10.1111\/j.0022-202X.2004.23550.x","volume":"124","author":"Z Chen","year":"2005","unstructured":"Chen Z, Seo JY, Kim YK, et\u00a0al. Heat modulation of tropoelastin, fibrillin-1, and matrix metalloproteinase-12 in human skin in vivo. J Invest Dermatol. 2005;124:70\u20138.","journal-title":"J Invest Dermatol"},{"key":"603_CR36","doi-asserted-by":"publisher","first-page":"28869","DOI":"10.1074\/jbc.M113.497727","volume":"288","author":"MD Combs","year":"2013","unstructured":"Combs MD, Knutsen RH, Broekelmann TJ, et\u00a0al. Microfibril-associated glycoprotein 2 (MAGP2) loss of function has pleiotropic effects in vivo. J Biol Chem. 2013;288:28869\u201380.","journal-title":"J Biol Chem"},{"key":"603_CR37","doi-asserted-by":"publisher","first-page":"800","DOI":"10.1074\/jbc.M609692200","volume":"282","author":"R Lemaire","year":"2007","unstructured":"Lemaire R, Bayle J, Mecham RP, Lafyatis R. Microfibril-associated MAGP-2 stimulates elastic fiber assembly. J Biol Chem. 2007;282:800\u20138.","journal-title":"J Biol Chem"},{"key":"603_CR38","doi-asserted-by":"publisher","first-page":"574","DOI":"10.1111\/j.1600-0625.2006.00442.x","volume":"15","author":"V Cenizo","year":"2006","unstructured":"Cenizo V, Andr\u00e9 V, Reymermier C, et\u00a0al. LOXL as a target to increase the elastin content in adult skin: a dill extract induces the LOXL gene expression. Exp Dermatol. 2006;15:574\u201381.","journal-title":"Exp Dermatol"},{"issue":"3","key":"603_CR39","first-page":"175","volume":"69","author":"S Pain","year":"2018","unstructured":"Pain S, Berth\u00e9l\u00e9my N, Naudin C, Degrave V, Andr\u00e9-Frei V. Understanding solar skin elastosis\u2014cause and treatment. J Cosmet Sci. 2018;69(3):175\u201385.","journal-title":"J Cosmet Sci"},{"issue":"2","key":"603_CR40","doi-asserted-by":"publisher","first-page":"85","DOI":"10.1159\/000097655","volume":"20","author":"KR Mewes","year":"2007","unstructured":"Mewes KR, Raus M, Bernd A, et\u00a0al. Elastin expression in a newly developed full-thickness skin equivalent. Skin Pharmacol Physiol. 2007;20(2):85\u201395.","journal-title":"Skin Pharmacol Physiol"},{"key":"603_CR41","doi-asserted-by":"publisher","first-page":"81","DOI":"10.1016\/j.addr.2013.12.006","volume":"69\u201370","author":"SH Mathes","year":"2014","unstructured":"Mathes SH, Ruffner H, Graf-Hausner U. The use of skin models in drug development. Adv Drug Deliv Rev. 2014;69\u201370:81\u2013102.","journal-title":"Adv Drug Deliv Rev"},{"issue":"9","key":"603_CR42","first-page":"E53","volume":"13","author":"T Gambichler","year":"2020","unstructured":"Gambichler T, Mamali K, Scheel C. A brief literature update on mid-dermal elastolysis with an emphasis on pathogenetic and therapeutic aspects. J Clin Aesthet Dermatol. 2020;13(9):E53\u20138.","journal-title":"J Clin Aesthet Dermatol"},{"issue":"2","key":"603_CR43","doi-asserted-by":"publisher","first-page":"526","DOI":"10.1111\/jocd.13865","volume":"20","author":"AD Widgerow","year":"2021","unstructured":"Widgerow AD, Napekoski K. New approaches to skin photodamage histology\u2014differentiating \u201cgood\u201d versus \u201cbad\u201d Elastin. J Cosmet Dermatol. 2021;20(2):526\u201331. https:\/\/doi.org\/10.1111\/jocd.13865.","journal-title":"J Cosmet Dermatol"},{"issue":"26","key":"603_CR44","doi-asserted-by":"publisher","first-page":"6881","DOI":"10.1007\/s00216-018-1288-z","volume":"410","author":"P Rathod","year":"2018","unstructured":"Rathod P, Kaur M, Ho HP, et\u00a0al. Quantification of desmosine and isodesmosine using MALDI-ion trap tandem mass spectrometry. Anal Bioanal Chem. 2018;410(26):6881\u20139. https:\/\/doi.org\/10.1007\/s00216-018-1288-z.","journal-title":"Anal Bioanal Chem"},{"key":"603_CR45","doi-asserted-by":"publisher","DOI":"10.1039\/D0AN02039G","author":"M Egawa","year":"2021","unstructured":"Egawa M. Raman microscopy for skin evaluation. Analyst. 2021. https:\/\/doi.org\/10.1039\/D0AN02039G.","journal-title":"Analyst"},{"issue":"7","key":"603_CR46","doi-asserted-by":"publisher","first-page":"588","DOI":"10.1089\/ten.TEC.2013.0293","volume":"20","author":"TL Fernandez","year":"2014","unstructured":"Fernandez TL, Lonkhuyzen DRV, Dawson RA, et\u00a0al. Characterization of a human skin equivalent model to study the effects of ultraviolet b radiation on keratinocytes. Tiss Eng. 2014;20(7):588\u201398. https:\/\/doi.org\/10.1089\/ten.TEC.2013.0293.","journal-title":"Tiss Eng."},{"issue":"1","key":"603_CR47","doi-asserted-by":"publisher","first-page":"35","DOI":"10.1111\/j.1755-148X.2010.00786.x","volume":"24","author":"CA Brohem","year":"2011","unstructured":"Brohem CA, Cardeal LB, Tiago M, et\u00a0al. Artificial skin in perspective: concepts and applications. Pigment Cell Melanoma Res. 2011;24(1):35\u201350.","journal-title":"Pigment Cell Melanoma Res"},{"key":"603_CR48","doi-asserted-by":"publisher","first-page":"368","DOI":"10.1046\/j.1523-1747.2003.12364.x","volume":"121","author":"P Koria","year":"2003","unstructured":"Koria P, Brazeau D, Kirkwood K, et\u00a0al. Gene expression profile of tissue engineered skin subjected to acute barrier disruption. J Invest Dermatol. 2003;121:368\u201382.","journal-title":"J Invest Dermatol"},{"issue":"4","key":"603_CR49","doi-asserted-by":"publisher","first-page":"402","DOI":"10.1006\/meth.2001.1262","volume":"25","author":"KJ Livak","year":"2001","unstructured":"Livak KJ, Schimittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2\u2212\u0394\u0394CT method. Methods. 2001;25(4):402\u20138.","journal-title":"Methods"},{"issue":"6","key":"603_CR50","doi-asserted-by":"publisher","first-page":"1101","DOI":"10.1038\/nprot.2008.73","volume":"3","author":"TD Schmittgen","year":"2008","unstructured":"Schmittgen TD, Livak KJ. Analyzing real-time PCR data by the comparative C(T) method. Nat Protoc. 2008;3(6):1101\u20138.","journal-title":"Nat Protoc"}],"container-title":["Dermatology and Therapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s13555-021-00603-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s13555-021-00603-y\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s13555-021-00603-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,23]],"date-time":"2021-11-23T23:07:11Z","timestamp":1637708831000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s13555-021-00603-y"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,10,14]]},"references-count":50,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2021,12]]}},"alternative-id":["603"],"URL":"https:\/\/doi.org\/10.1007\/s13555-021-00603-y","relation":{},"ISSN":["2193-8210","2190-9172"],"issn-type":[{"value":"2193-8210","type":"print"},{"value":"2190-9172","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,10,14]]},"assertion":[{"value":"21 July 2021","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"21 August 2021","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"14 October 2021","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}}]}}