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To examine involvement of the disease with periodontal disease development, we established immortalized periodontal ligament cells obtained from a Down\u2019s syndrome patient by use of <jats:italic>SV40T-Ag<\/jats:italic> and <jats:italic>hTERT<\/jats:italic> gene transfection. Expressions of <jats:italic>SV40T-Ag<\/jats:italic> and <jats:italic>hTERT<\/jats:italic> were observed in periodontal ligament cell-derived immortalized cells established from healthy (STPDL) and Down\u2019s syndrome patient (STPDLDS) samples. Primary cultured periodontal ligament cells obtained from a healthy subject (pPDL) had a limited number of population doublings (&lt;\u200940), while STPDL and STPDLDS cells continued to grow with more than 80 population doublings. Primary cultured periodontal ligament cells obtained from the patient showed a chromosome pattern characteristic of Down\u2019s syndrome with trisomy 21, whereas STPDLDS samples showed a large number of abnormal chromosomes in those results. Gene expression analysis revealed that expression of <jats:italic>DSCR-1<\/jats:italic> in STPDLDS is greater than that in STPDL. These results suggest that the newly established STPDLDS cell line may be a useful tool for study of periodontal disease in Down\u2019s syndrome patients.<\/jats:p>","DOI":"10.1007\/s13577-021-00621-0","type":"journal-article","created":{"date-parts":[[2021,9,29]],"date-time":"2021-09-29T18:11:24Z","timestamp":1632939084000},"page":"379-383","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Establishment of Down\u2019s syndrome periodontal ligament cells by transfection with SV40T-Ag and hTERT"],"prefix":"10.1007","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7953-5600","authenticated-orcid":false,"given":"Takeyoshi","family":"Asakawa","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Atsushi","family":"Yamada","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masumi","family":"Kugino","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomokazu","family":"Hasegawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kentaro","family":"Yoshimura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kiyohito","family":"Sasa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mitsuhiro","family":"Kinoshita","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masakazu","family":"Nitta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karin","family":"Nagata","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomomi","family":"Sugiyama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryutaro","family":"Kamijo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takahiro","family":"Funatsu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2021,9,29]]},"reference":[{"key":"621_CR1","doi-asserted-by":"publisher","first-page":"3358","DOI":"10.1111\/cas.14160","volume":"110","author":"Y Kubota","year":"2019","unstructured":"Kubota Y, Uryu K, Ito T, et al. 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The study protocol was approved by the ethics committee of Showa University (No2013-007).","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval"}}]}}