{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T17:22:23Z","timestamp":1776100943026,"version":"3.50.1"},"reference-count":26,"publisher":"Springer Science and Business Media LLC","issue":"4","license":[{"start":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T00:00:00Z","timestamp":1776038400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T00:00:00Z","timestamp":1776038400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Human Cell"],"DOI":"10.1007\/s13577-026-01371-7","type":"journal-article","created":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T16:27:55Z","timestamp":1776097675000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Extracellular vesicle-derived CDCP1 promotes chemoresistance and macrophage polarization in breast cancer"],"prefix":"10.1007","volume":"39","author":[{"given":"Yibing","family":"Liu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Ma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ting","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Di","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yonglei","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2026,4,13]]},"reference":[{"issue":"1","key":"1371_CR1","doi-asserted-by":"publisher","first-page":"1722433","DOI":"10.1080\/20013078.2020.1722433","volume":"9","author":"R Crescitelli","year":"2020","unstructured":"Crescitelli R, L\u00e4sser C, Jang SC, Cvjetkovic A, Malmh\u00e4ll C, Karimi N, et al. Subpopulations of extracellular vesicles from human metastatic melanoma tissue identified by quantitative proteomics after optimized isolation. J Extracell Vesicles. 2020;9(1):1722433.","journal-title":"J Extracell Vesicles"},{"issue":"3","key":"1371_CR2","doi-asserted-by":"publisher","first-page":"1548","DOI":"10.1038\/s41596-020-00466-1","volume":"16","author":"R Crescitelli","year":"2021","unstructured":"Crescitelli R, L\u00e4sser C, L\u00f6tvall J. Isolation and characterization of extracellular vesicle subpopulations from tissues. Nat Protoc. 2021;16(3):1548\u201380.","journal-title":"Nat Protoc"},{"issue":"14","key":"1371_CR3","doi-asserted-by":"publisher","first-page":"1426","DOI":"10.1161\/CIRCULATIONAHA.120.049254","volume":"143","author":"S Sahoo","year":"2021","unstructured":"Sahoo S, Adamiak M, Mathiyalagan P, Kenneweg F, Kafert-Kasting S, Thum T. Therapeutic and Diagnostic Translation of Extracellular Vesicles in Cardiovascular Diseases: Roadmap to the Clinic. Circulation. 2021;143(14):1426\u201349.","journal-title":"Circulation"},{"key":"1371_CR4","doi-asserted-by":"publisher","first-page":"33","DOI":"10.3389\/fmolb.2020.00033","volume":"7","author":"C Jensen","year":"2020","unstructured":"Jensen C, Teng Y. Is It Time to Start Transitioning From 2D to 3D Cell Culture? Front Mol Biosci. 2020;7:33.","journal-title":"Front Mol Biosci"},{"issue":"1","key":"1371_CR5","doi-asserted-by":"publisher","first-page":"69","DOI":"10.1038\/s41551-019-0485-1","volume":"4","author":"Z Yang","year":"2020","unstructured":"Yang Z, Shi J, Xie J, Wang Y, Sun J, Liu T, et al. Large-scale generation of functional mRNA-encapsulating exosomes via cellular nanoporation. Nat Biomed Eng. 2020;4(1):69\u201383.","journal-title":"Nat Biomed Eng"},{"issue":"3","key":"1371_CR6","doi-asserted-by":"publisher","first-page":"607","DOI":"10.1002\/ijc.31080","volume":"142","author":"K Jingushi","year":"2018","unstructured":"Jingushi K, Uemura M, Ohnishi N, Nakata W, Fujita K, Naito T, et al. Extracellular vesicles isolated from humanrenal cell carcinoma tissues disrupt vascular endothelial cell morphology viaazurocidin. Int J Cancer. 2018;142(3):607\u201317.","journal-title":"Int J Cancer."},{"issue":"24","key":"1371_CR7","doi-asserted-by":"publisher","first-page":"9366","DOI":"10.3390\/ijms21249366","volume":"21","author":"T Camino","year":"2020","unstructured":"Camino T, Lago-Baameiro N, Martis-Sueiro A, Couto I, Santos F, Baltar J, et al. Deciphering Adipose Tissue Extracellular Vesicles Protein Cargo and ItsRole in Obesity. Int J Mol Sci. 2020;21(24):9366.","journal-title":"Int J Mol Sci"},{"issue":"1","key":"1371_CR8","doi-asserted-by":"publisher","first-page":"1635420","DOI":"10.1080\/20013078.2019.1635420","volume":"8","author":"SC Jang","year":"2019","unstructured":"Jang SC, Crescitelli R, Cvjetkovic A, Belgrano V, Olofsson Bagge R, SundfeldtK Ochiya T, et al. Mitochondrial protein enriched extracellularvesicles discovered in human melanoma tissues can be detected in patient plasma. J Extracell Vesicles. 2019;8(1):1635420.","journal-title":"J Extracell Vesicles."},{"issue":"1","key":"1371_CR9","doi-asserted-by":"publisher","first-page":"3854","DOI":"10.1038\/s41467-019-11486-1","volume":"10","author":"D Wu","year":"2019","unstructured":"Wu D, Yan J, Shen X, Sun Y, Thulin M, Cai Y, et al. Profilingsurface proteins on individual exosomes using a proximity barcoding assay. Nat Commun. 2019;10(1):3854.","journal-title":"Nat Commun."},{"issue":"34","key":"1371_CR10","doi-asserted-by":"publisher","first-page":"22410","DOI":"10.1021\/acsomega.1c03248","volume":"6","author":"D Yang","year":"2021","unstructured":"Yang D, Li Z, Gao G, Li X, Liao Z, Wang Y, et al. Combined Analysis of Surface Protein Profile and microRNA Expression Profile of Exosomes Derived from Brain Microvascular Endothelial Cells in Early Cerebral Ischemia. ACS Omega. 2021;6(34):22410\u201321.","journal-title":"ACS Omega"},{"issue":"8","key":"1371_CR11","doi-asserted-by":"publisher","DOI":"10.1002\/jev2.12085","volume":"10","author":"M Kang","year":"2021","unstructured":"Kang M, Jordan V, Blenkiron C, Chamley LW. Biodistribution of extracellular vesicles following administration into animals: A systematic review. J Extracell Vesicles. 2021;10(8):e12085.","journal-title":"J Extracell Vesicles"},{"issue":"1","key":"1371_CR12","doi-asserted-by":"publisher","first-page":"504","DOI":"10.1186\/s12885-018-4418-2","volume":"18","author":"M Barok","year":"2018","unstructured":"Barok M, Puhka M, Vereb G, Szollosi J, Isola J, Joensuu H. Cancer-derived exosomes from HER2-positive cancer cells carry trastuzumab-emtansine into cancer cells leading to growth inhibition and caspase activation. BMC Cancer. 2018;18(1):504.","journal-title":"BMC Cancer"},{"issue":"9","key":"1371_CR13","doi-asserted-by":"publisher","first-page":"2259","DOI":"10.1158\/0008-5472.CAN-20-2978","volume":"81","author":"T Khan","year":"2021","unstructured":"Khan T, Kryza T, Lyons NJ, He Y, Hooper JD. The CDCP1 Signaling Hub: A Target for Cancer Detection and Therapeutic Intervention. Cancer Res. 2021;81(9):2259\u201369.","journal-title":"Cancer Res"},{"issue":"7","key":"1371_CR14","doi-asserted-by":"publisher","first-page":"723","DOI":"10.1002\/iub.198","volume":"61","author":"A Wortmann","year":"2009","unstructured":"Wortmann A, He Y, Deryugina EI, Quigley JP, Hooper JD. The cell surface glycoprotein CDCP1 in cancer\u2013insights, opportunities, and challenges. IUBMB Life. 2009;61(7):723\u201330.","journal-title":"IUBMB Life"},{"key":"1371_CR15","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2022.120600","volume":"301","author":"X Qi","year":"2022","unstructured":"Qi X, Gao J, Li Z, Zhang G, Li J, Fu Y, et al. CDCP1: Apromising diagnostic biomarker and therapeutic target for human cancer. LifeSci. 2022;301:120600.","journal-title":"LifeSci"},{"issue":"7","key":"1371_CR16","doi-asserted-by":"publisher","first-page":"776","DOI":"10.1038\/s41589-021-00783-w","volume":"17","author":"T Kryza","year":"2021","unstructured":"Kryza T, Khan T, Lovell S, Harrington BS, Yin J, Porazinski S, et al. Substrate-biased activity-based probes identify proteases that cleave receptor CDCP1. Nat Chem Biol. 2021;17(7):776\u201383.","journal-title":"Nat Chem Biol"},{"issue":"4","key":"1371_CR17","doi-asserted-by":"publisher","DOI":"10.26508\/lsa.202000832","volume":"4","author":"K Kajiwara","year":"2021","unstructured":"Kajiwara K, Yamano S, Aoki K, Okuzaki D, Matsumoto K, Okada M. CDCP1 promotes compensatory renal growth by integrating Src and Met signaling. Life Sci Alliance. 2021;4(4):e202000832.","journal-title":"Life Sci Alliance"},{"issue":"1","key":"1371_CR18","doi-asserted-by":"publisher","first-page":"8983","DOI":"10.1038\/s41598-022-12995-8","volume":"12","author":"Y Murakami","year":"2022","unstructured":"Murakami Y, Kusakabe D, Watari K, Kawahara A, Azuma K, Akiba J, et al. AXL\/CDCP1\/SRC axis confers acquired resistance to osimertinibin lung cancer. Sci Rep. 2022;12(1):8983.","journal-title":"Sci Rep"},{"issue":"1","key":"1371_CR19","doi-asserted-by":"publisher","first-page":"219","DOI":"10.1038\/s41388-019-0983-3","volume":"39","author":"Y He","year":"2020","unstructured":"He Y, Davies CM, Harrington BS, Hellmers L, Sheng Y, Broomfield A, et al. CDCP1 enhances Wnt signaling in colorectal cancer promoting nuclear localization of \u03b2-catenin and E-cadherin. Oncogene. 2020;39(1):219\u201333.","journal-title":"Oncogene"},{"issue":"10","key":"1371_CR20","doi-asserted-by":"publisher","first-page":"1449","DOI":"10.1158\/1541-7786.MCR-13-0587","volume":"12","author":"T Uekita","year":"2014","unstructured":"Uekita T, Fujii S, Miyazawa Y, Iwakawa R, Narisawa-Saito M, Nakashima K, et al. Oncogenic Ras\/ERK signalingactivates CDCP1 to promote tumor invasion and metastasis. Mol Cancer Res. 2014;12(10):1449\u201359.","journal-title":"Mol Cancer Res"},{"key":"1371_CR21","doi-asserted-by":"publisher","first-page":"1469354","DOI":"10.1155\/2022\/1469354","volume":"2022","author":"J Zhao","year":"2022","unstructured":"Zhao J, Mei J, Wang F, Zhao X, Ren Y, Zhao X, et al. Overexpression of CDCP1 is Associated with Poor Prognosis and Enhanced Immune Checkpoints Expressions in Breast Cancer. J Oncol. 2022;2022:1469354.","journal-title":"J Oncol"},{"issue":"1","key":"1371_CR22","doi-asserted-by":"publisher","first-page":"1453","DOI":"10.1038\/s41467-021-21774-4","volume":"12","author":"D Sch\u00e4fer","year":"2021","unstructured":"Sch\u00e4fer D, Tomiuk S, K\u00fcster LN, Rawashdeh WA, Henze J, Tischler-H\u00f6hleG Agorku DJ, et al. Identification of CD318, TSPAN8 and CD66c as target candidates for CAR T cell based immunotherapy of pancreatic adenocarcinoma. Nat Commun. 2021;12(1):1453.","journal-title":"Nat Commun."},{"issue":"12","key":"1371_CR23","doi-asserted-by":"publisher","first-page":"2819","DOI":"10.4049\/jimmunol.2001406","volume":"206","author":"Y Lun","year":"2021","unstructured":"Lun Y, Borjini N, Miura NN, Ohno N, Singer NG, Lin F. CDCP1 on Dendritic Cells Contributes to the Development of a Model of Kawasaki Disease. J Immunol. 2021;206(12):2819\u201327.","journal-title":"J Immunol"},{"issue":"11","key":"1371_CR24","doi-asserted-by":"publisher","first-page":"4871","DOI":"10.7150\/thno.43539","volume":"10","author":"W Lin","year":"2020","unstructured":"Lin W, Xu Y, Chen X, Liu J, Weng Y, Zhuang Q, et al. Radiation-induced small extracellular vesicles as \u201ccarriages\u201d promote tumor antigen release and trigger antitumor immunity. Theranostics. 2020;10(11):4871\u201384.","journal-title":"Theranostics"},{"issue":"12","key":"1371_CR25","doi-asserted-by":"publisher","first-page":"1783","DOI":"10.1038\/sj.onc.1206220","volume":"22","author":"JD Hooper","year":"2003","unstructured":"Hooper JD, Zijlstra A, Aimes RT, Liang H, Claassen GF, Tarin D, et al. Subtractive immunization using highly metastatic human tumor cells identifies SIMA135\/CDCP1, a 135 kDa cell surface phosphorylated glycoprotein antigen. Oncogene. 2003;22(12):1783\u201394.","journal-title":"Oncogene"},{"issue":"8","key":"1371_CR26","doi-asserted-by":"publisher","first-page":"1197","DOI":"10.1158\/1541-7786.MCR-09-0100","volume":"7","author":"EI Deryugina","year":"2009","unstructured":"Deryugina EI, Conn EM, Wortmann A, Partridge JJ, Kupriyanova TA, Ardi VC, et al. Functional role of cell surface CUB domain-containing protein 1 in tumor cell dissemination. Mol Cancer Res. 2009;7(8):1197\u2013211.","journal-title":"Mol Cancer Res"}],"container-title":["Human Cell"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s13577-026-01371-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s13577-026-01371-7","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s13577-026-01371-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T16:27:55Z","timestamp":1776097675000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s13577-026-01371-7"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,13]]},"references-count":26,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2026,4]]}},"alternative-id":["1371"],"URL":"https:\/\/doi.org\/10.1007\/s13577-026-01371-7","relation":{},"ISSN":["1749-0774"],"issn-type":[{"value":"1749-0774","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,13]]},"assertion":[{"value":"2 November 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"18 March 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"13 April 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare that they have no conflict of interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"The study was approved by the local Ethic Committee.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval and consent to participate"}},{"value":"All the listed authors have participated in the study and approved the submitted manuscript.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The human breast cancer cell lines MDA-MB-231 as well as the human monocytic cell line THP-1, used in this study were obtained from Cell Bank of Chinese Academy of Sciences. All cell lines were authenticated by short tandem repeat (STR) profiling within the last three years and tested negative for mycoplasma contamination.","order":5,"name":"Ethics","group":{"name":"EthicsHeading","label":"Cell Line Authentication"}}],"article-number":"65"}}