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In the present study, we evaluated the recovery and amplification rates of mitochondrial DNA from larvae of the forensically important blow fly\n                    <jats:italic>Chrysomya megacephala<\/jats:italic>\n                    (F.) (Diptera, Calliphoridae), preserved for up to 47\u00a0days in the solutions commonly used in routine forensic investigations. Some larvae (\n                    <jats:italic>N<\/jats:italic>\n                    \u2009=\u200988) were immediately placed in the preservative solution, while another group of larvae (\n                    <jats:italic>N<\/jats:italic>\n                    \u2009=\u200988) was killed by immersion in water heated up to 80\u00a0\u00b0C for 30\u00a0s before being preserved at room temperature. In general, it was possible to recover highly pure DNA from larvae subjected to almost all treatments, with yields varying proportionally across different storage intervals. Among the solutions that yielded the highest DNA and consequently achieved successful amplification rates were 99.3% ethanol (&gt;\u2009100\u00a0ng\/\u00b5L, with rates of 95%) and 70% ethanol (20\u2013100\u00a0ng\/\u00b5L, with rates of 90%), primarily up to the 28-day storage interval. The worst DNA yields and low amplification rates (approximately 50%) were associated with the 47-day storage interval, despite the preservative method used, except for Kahle\u2019s solution, which presents no amplification at all. Our results suggest that ethanol at concentrations of 70\u201399.3% is the most efficient preservative for preserving the integrity and usability of forensically important larvae DNA for diagnostic purposes, particularly within a range of up to 28\u00a0days.\n                  <\/jats:p>","DOI":"10.1007\/s13744-026-01366-x","type":"journal-article","created":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T03:27:15Z","timestamp":1771558035000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["DNA Recovery from Forensically Relevant Blow Fly Larvae (Insecta, Diptera, Calliphoridae) Kept in Different Preservative Solutions"],"prefix":"10.1007","volume":"55","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-0705-3871","authenticated-orcid":false,"given":"Jos\u00e9 Lucas","family":"Dias-Silva","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-3168-4972","authenticated-orcid":false,"given":"Orianna","family":"Tamara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4829-8577","authenticated-orcid":false,"given":"Andr\u00e9s F.","family":"Maya-Duque","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0962-777X","authenticated-orcid":false,"given":"Eduardo","family":"Amat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0826-4492","authenticated-orcid":false,"given":"Luz Miryam","family":"Gomez-Pi\u00f1erez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-8272-0971","authenticated-orcid":false,"given":"Jo\u00e3o Vitor","family":"Almeida-Santos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2500-1774","authenticated-orcid":false,"given":"Carina Mara","family":"Souza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2711-5230","authenticated-orcid":false,"given":"Tais","family":"Madeira-Ott","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3843-4974","authenticated-orcid":false,"given":"Aline Marrara","family":"Prado","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7343-2419","authenticated-orcid":false,"given":"Patricia Jacqueline","family":"Thyssen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,2,20]]},"reference":[{"issue":"1\u20133","key":"1366_CR1","doi-asserted-by":"publisher","first-page":"50","DOI":"10.1016\/j.forsciint.2003.08.010","volume":"138","author":"ZJ Adams","year":"2003","unstructured":"Adams ZJ, Hall MJ (2003) Methods used for the killing and preservation of blowfly larvae, and their effect on post-mortem larval length. 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