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In Brazil, the control of this sand fly species is difficult due to its resistance to chemical insecticides. The present study had the objectives of determining the chemical composition of\n                    <jats:italic>Piper marginatum<\/jats:italic>\n                    essential oil (EO) and to evaluating its ovicidal and larvicidal activity against\n                    <jats:italic>Lu. longipalpis<\/jats:italic>\n                    under laboratory conditions. To achieve this, a gas chromatography-mass spectrometry (GC-MS) was performed, and nine concentrations of the EO were tested on eggs and larvae: 0.25%, 0.5%, 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, and 4%. Probit analysis estimated LC\u2085\u2080 and LC\u2089\u2080 values of 1.18% and 2.41% for eggs, and 0.91% and 1.71% for larvae, respectively. The major constituent of\n                    <jats:italic>P. marginatum<\/jats:italic>\n                    EO was 3',4'-methylenedioxypropiophenone, a phenylpropanoid commonly found in\n                    <jats:italic>Piper<\/jats:italic>\n                    species. All concentrations higher than 1% killed more than 50% of the treated larvae and rendered more than 50% of the eggs unviable. This is the first report of the toxicity of EO from a\n                    <jats:italic>Piper<\/jats:italic>\n                    plant against a sand fly species. This EO may constitute a viable alternative for controlling the immature stages of the primary vector of VL.\n                  <\/jats:p>","DOI":"10.1007\/s13744-026-01422-6","type":"journal-article","created":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T14:39:55Z","timestamp":1787150395000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Ovicidal and Larvicidal Effects of Piper marginatum Essential Oil on Lutzomyia longipalpis (Lutz &amp; Neiva, 1912)"],"prefix":"10.1007","volume":"55","author":[{"given":"Lucas Alexandre Farias","family":"de Souza","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michele Trombin","family":"de Souza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mireli Trombin","family":"de Souza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Damaso","family":"Rubio-Vargas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo\u00e3o Carlos","family":"Minozzo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beatriz Helena Lameiro","family":"de Noronha Sales Maia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0215-2596","authenticated-orcid":false,"given":"Magda Clara Vieira","family":"da Costa-Ribeiro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,8,19]]},"reference":[{"key":"1422_CR1","doi-asserted-by":"publisher","first-page":"1272","DOI":"10.1111\/j.1365-3156.2009.02371.x","volume":"14","author":"B Alexander","year":"2009","unstructured":"Alexander B, Barros VC, de Souza SF et al (2009) Susceptibility to chemical insecticides of two Brazilian populations of the visceral leishmaniasis vector Lutzomyia longipalpis (Diptera: Psychodidae). 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