{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T17:50:11Z","timestamp":1777917011412,"version":"3.51.4"},"reference-count":165,"publisher":"Springer Science and Business Media LLC","license":[{"start":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T00:00:00Z","timestamp":1775174400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T00:00:00Z","timestamp":1775174400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Acta Neurol Belg"],"DOI":"10.1007\/s13760-026-03033-9","type":"journal-article","created":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T05:26:17Z","timestamp":1775193977000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["CRISPR-Cas9 and next-generation gene editing strategies for therapeutic intervention of neurodegenerative pathways in Alzheimer\u2019s disease: a state-of-the-art review"],"prefix":"10.1007","author":[{"given":"Muhammad Sohail","family":"Khan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9246-0850","authenticated-orcid":false,"given":"Imran","family":"Zafar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8724-5566","authenticated-orcid":false,"given":"Adil","family":"Jamal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6618-9422","authenticated-orcid":false,"given":"Fayez Saeed","family":"Bahwerth","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samiullah","family":"Khan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muhammad","family":"Faizan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hanif","family":"Khan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-2595-9992","authenticated-orcid":false,"given":"Muhammad","family":"Noman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kendrix","family":"Okeibunor","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ashfaq","family":"Ahmad","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muhammad","family":"Zain Ul Abedin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2026,4,3]]},"reference":[{"key":"3033_CR1","doi-asserted-by":"crossref","unstructured":"Chaudhary RK, Mateti UV, Khanal P, Rawal KB, Jain P, Patil VS et al (2024) Alzheimer\u2019s Disease: Epidemiology, Neuropathology, and Neurochemistry. Computational and Experimental Studies in Alzheimer\u2019s disease. ed: CRC, pp 1\u201314","DOI":"10.1201\/9781003412069-1"},{"key":"3033_CR2","doi-asserted-by":"publisher","first-page":"503","DOI":"10.1186\/s12888-025-06661-2","volume":"25","author":"D-T Yu","year":"2025","unstructured":"Yu D-T, Li R-X, Sun J-R, Rong X-W, Guo X-G, Zhu G-D (2025) Global mortality, prevalence and disability-adjusted life years of Alzheimer\u2019s disease and other dementias in adults aged 60 years or older, and the impact of the COVID-19 pandemic: a comprehensive analysis for the global burden of disease 2021. BMC Psychiatry 25:503","journal-title":"BMC Psychiatry"},{"key":"3033_CR3","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2023.e22204","author":"Y Arfat","year":"2023","unstructured":"Arfat Y, Zafar I, Sehgal SA, Ayaz M, Sajid M, Khan JM et al (2023) In silico designing of multiepitope-based-peptide (MBP) vaccine against MAPK protein express for Alzheimer\u2019s disease in Zebrafish. Heliyon. https:\/\/doi.org\/10.1016\/j.heliyon.2023.e22204","journal-title":"Heliyon"},{"key":"3033_CR4","doi-asserted-by":"publisher","first-page":"5169","DOI":"10.3390\/ijms25105169","volume":"25","author":"J Li","year":"2024","unstructured":"Li J, Haj Ebrahimi A, Ali AB (2024) Advances in therapeutics to alleviate cognitive decline and neuropsychiatric symptoms of Alzheimer\u2019s disease. Int J Mol Sci 25:5169","journal-title":"Int J Mol Sci"},{"key":"3033_CR5","doi-asserted-by":"publisher","first-page":"37","DOI":"10.1186\/s41983-025-00968-3","volume":"61","author":"Tb Ameen","year":"2025","unstructured":"Ameen Tb, Ali U, Salma O, Abdul Samee M, Iraj Abbas SM, Naveera Kashif S et al (2025) Amyloid solutions: lecanemab, gantenerumab, and donanemab in the treatment of Alzheimer\u2019s disease. Egypt J Neurol Psychiatry Neurosurg 61:37","journal-title":"Egypt J Neurol Psychiatry Neurosurg"},{"key":"3033_CR6","doi-asserted-by":"publisher","first-page":"34","DOI":"10.1212\/CON.0000000000000687","volume":"25","author":"MF Mendez","year":"2019","unstructured":"Mendez MF (2019) Early-onset Alzheimer disease and its variants. Continuum Lifelong Learn Neurol 25:34\u201351","journal-title":"Continuum Lifelong Learn Neurol"},{"key":"3033_CR7","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/alzrt59","volume":"3","author":"RJ Bateman","year":"2011","unstructured":"Bateman RJ, Aisen PS, De Strooper B, Fox NC, Lemere CA, Ringman JM et al (2011) Autosomal-dominant Alzheimer\u2019s disease: a review and proposal for the prevention of Alzheimer\u2019s disease. Alzheimers Res Ther 3:1","journal-title":"Alzheimers Res Ther"},{"key":"3033_CR8","unstructured":"Li T (2024) Description and investigation of genetics, metabolism, and diet habit on Alzheimer\u2019s Disease-related cognitive impairment. Iowa State University"},{"key":"3033_CR9","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2023.104511","author":"SJ Andrews","year":"2023","unstructured":"Andrews SJ, Renton AE, Fulton-Howard B, Podlesny-Drabiniok A, Marcora E, Goate AM (2023) The complex genetic architecture of Alzheimer\u2019s disease: novel insights and future directions. EBioMedicine. https:\/\/doi.org\/10.1016\/j.ebiom.2023.104511","journal-title":"EBioMedicine"},{"key":"3033_CR10","doi-asserted-by":"publisher","first-page":"103","DOI":"10.1152\/physrev.00030.2022","volume":"104","author":"M Perluigi","year":"2024","unstructured":"Perluigi M, Di Domenico F, Butterfield DA (2024) Oxidative damage in neurodegeneration: roles in the pathogenesis and progression of Alzheimer disease. Physiol Rev 104:103\u2013197","journal-title":"Physiol Rev"},{"key":"3033_CR11","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2025.1578138","volume":"19","author":"MS Khan","year":"2025","unstructured":"Khan MS, Qureshi N, Khan R, Son Y-O, Maqbool T (2025) CRISPR\/Cas9-based therapeutics as a promising strategy for management of Alzheimer\u2019s disease: progress and prospects. Front Cell Neurosci 19:1578138","journal-title":"Front Cell Neurosci"},{"key":"3033_CR12","doi-asserted-by":"publisher","first-page":"767","DOI":"10.1016\/j.stemcr.2024.05.004","volume":"19","author":"MF Pereira","year":"2024","unstructured":"Pereira MF, Shyti R, Testa G (2024) In and out: benchmarking in vitro, in vivo, ex vivo, and xenografting approaches for an integrative brain disease modeling pipeline. Stem Cell Rep 19:767\u2013795","journal-title":"Stem Cell Rep"},{"key":"3033_CR13","doi-asserted-by":"publisher","DOI":"10.1016\/j.omtn.2025.102636","author":"N Kalter","year":"2025","unstructured":"Kalter N, Fuster-Garc\u00eda C, Silva A, Ronco-D\u00edaz V, Roncelli S, Turchiano G et al (2025) Off-target effects in CRISPR-Cas genome editing for human therapeutics: progress and challenges. Mol Ther Nucleic Acids. https:\/\/doi.org\/10.1016\/j.omtn.2025.102636","journal-title":"Mol Ther Nucleic Acids"},{"key":"3033_CR14","doi-asserted-by":"publisher","DOI":"10.1186\/s12885-025-14113-z","volume":"25","author":"S Ahmad","year":"2025","unstructured":"Ahmad S, Zafar I, Shafiq S, Sehar L, Khalil H, Matloob N et al (2025) Deep learning-based computational approach for predicting ncRNAs-disease associations in metaplastic breast cancer diagnosis. BMC Cancer 25:830","journal-title":"BMC Cancer"},{"key":"3033_CR15","doi-asserted-by":"publisher","DOI":"10.3390\/biomedicines11051238","volume":"11","author":"DM Sapozhnikov","year":"2023","unstructured":"Sapozhnikov DM, Szyf M (2023) Increasing specificity of targeted DNA methylation editing by non-enzymatic CRISPR\/dCas9-based steric hindrance. Biomedicines 11:1238","journal-title":"Biomedicines"},{"key":"3033_CR16","doi-asserted-by":"crossref","unstructured":"Shareena G, Viswalingam V, Rajput MS, Kumar D (2025) CRISPR\/Cas9 gene editing: A new hope for Alzheimer\u2019s disease. Targeted Therapy for the Central Nervous System. ed: Elsevier, pp 301\u2013323","DOI":"10.1016\/B978-0-443-23841-3.00014-5"},{"key":"3033_CR17","first-page":"19","volume":"20","author":"SG Yilmaz","year":"2021","unstructured":"Yilmaz SG (2021) Genome editing technologies: CRISPR, leaper, restore, arcut, sati, and rescue. EXCLI J 20:19","journal-title":"EXCLI J"},{"key":"3033_CR18","unstructured":"Perrotta N (2025) Real-world clinical outcomes in HER2 positive and negative metastatic breast cancer patients treated with Abemaciclib, Ribociclib and Trastuzumab deruxtecan"},{"key":"3033_CR19","doi-asserted-by":"publisher","DOI":"10.3390\/biomedicines12071523","volume":"12","author":"\u017b S\u0142yk","year":"2024","unstructured":"S\u0142yk \u017b, Stachowiak N, Ma\u0142ecki M (2024) Recombinant adeno-associated virus vectors for gene therapy of the central nervous system: delivery routes and clinical aspects. Biomedicines 12:1523","journal-title":"Biomedicines"},{"key":"3033_CR20","doi-asserted-by":"publisher","first-page":"7364","DOI":"10.3390\/ijms26157364","volume":"26","author":"M \u0218erban","year":"2025","unstructured":"\u0218erban M, Toader C, Covache-Busuioc R-A (2025) Precision neuro-oncology in glioblastoma: AI-guided CRISPR editing and real-time multi-omics for genomic brain surgery. Int J Mol Sci 26:7364","journal-title":"Int J Mol Sci"},{"key":"3033_CR21","doi-asserted-by":"publisher","first-page":"877","DOI":"10.1038\/s41587-023-01915-4","volume":"42","author":"M Fiumara","year":"2024","unstructured":"Fiumara M, Ferrari S, Omer-Javed A, Beretta S, Albano L, Canarutto D et al (2024) Genotoxic effects of base and prime editing in human hematopoietic stem cells. Nat Biotechnol 42:877\u2013891","journal-title":"Nat Biotechnol"},{"key":"3033_CR22","unstructured":"Barritt J (2025) N-terminally Truncated A\u03b2 Assembly in Alzheimer\u2019s Disease. Queen Mary University of London"},{"key":"3033_CR23","doi-asserted-by":"publisher","DOI":"10.3390\/genes16030345","volume":"16","author":"P Poniah","year":"2025","unstructured":"Poniah P, Abdul Rashed A, Abdul Jalil J, Ali EZ (2025) Clinical significance of early-onset Alzheimer\u2019s mutations in Asian and Western populations: a scoping review. Genes 16:345","journal-title":"Genes"},{"key":"3033_CR24","doi-asserted-by":"publisher","first-page":"261","DOI":"10.1038\/s41582-023-00789-z","volume":"19","author":"C Reitz","year":"2023","unstructured":"Reitz C, Pericak-Vance MA, Foroud T, Mayeux R (2023) A global view of the genetic basis of Alzheimer disease. Nat Rev Neurol 19:261\u2013277","journal-title":"Nat Rev Neurol"},{"key":"3033_CR25","doi-asserted-by":"crossref","unstructured":"Dubey PR, Kaur G, Shukla R (2025) Nano-mediated management of metal toxicity-induced neurodegeneration: a critical review. Mol Neurobiol, pp 1\u201320","DOI":"10.1007\/s12035-025-04782-z"},{"key":"3033_CR26","doi-asserted-by":"publisher","first-page":"259","DOI":"10.3390\/cimb47040259","volume":"47","author":"N Brezic","year":"2025","unstructured":"Brezic N, Gligorevic S, Sic A, Knezevic NN (2025) Protein misfolding and aggregation as a mechanistic link between chronic pain and neurodegenerative diseases. Curr Issues Mol Biol 47:259","journal-title":"Curr Issues Mol Biol"},{"key":"3033_CR27","first-page":"245","volume":"8","author":"AK Hodges","year":"2021","unstructured":"Hodges AK, Piers TM, Collier D, Cousins O, Pocock JM (2021) Pathways linking Alzheimer\u2019s disease risk genes expressed highly in microglia. Neuroimmunol Neuroinflammation 8:245\u2013268","journal-title":"Neuroimmunol Neuroinflammation"},{"key":"3033_CR28","doi-asserted-by":"publisher","first-page":"215","DOI":"10.1186\/s12974-022-02580-1","volume":"19","author":"C Gao","year":"2022","unstructured":"Gao C, Shen X, Tan Y, Chen S (2022) Pathogenesis, therapeutic strategies and biomarker development based on omics analysis related to microglia in Alzheimer\u2019s disease. J Neuroinflamm 19:215","journal-title":"J Neuroinflamm"},{"key":"3033_CR29","doi-asserted-by":"publisher","first-page":"EMBR201846171","DOI":"10.15252\/embr.201846171","volume":"19","author":"C Sousa","year":"2018","unstructured":"Sousa C, Golebiewska A, Poovathingal SK, Kaoma T, Pires-Afonso Y, Martina S et al (2018) Single\u2010cell transcriptomics reveals distinct inflammation\u2010induced microglia signatures. EMBO Rep 19:EMBR201846171","journal-title":"EMBO Rep"},{"key":"3033_CR30","doi-asserted-by":"publisher","first-page":"1381","DOI":"10.1038\/s41593-025-01931-4","volume":"28","author":"L Fumagalli","year":"2025","unstructured":"Fumagalli L, Nazlie Mohebiany A, Premereur J, Polanco Miquel P, Bijnens B, Van de Walle P et al (2025) Microglia heterogeneity, modeling and cell-state annotation in development and neurodegeneration. Nat Neurosci 28:1381\u20131392","journal-title":"Nat Neurosci"},{"key":"3033_CR31","doi-asserted-by":"publisher","DOI":"10.3390\/biomedicines13020279","volume":"13","author":"D Dias","year":"2025","unstructured":"Dias D, Socodato R (2025) Beyond amyloid and tau: the critical role of microglia in Alzheimer\u2019s disease therapeutics. Biomedicines 13:279","journal-title":"Biomedicines"},{"key":"3033_CR32","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2023.1305933","volume":"14","author":"L Fornari Laurindo","year":"2024","unstructured":"Fornari Laurindo L, Aparecido Dias J, Cressoni Araujo A, Torres Pomini K, Machado Galhardi C, Rucco Penteado Detregiachi C et al (2024) Immunological dimensions of neuroinflammation and microglial activation: exploring innovative immunomodulatory approaches to mitigate neuroinflammatory progression. Front Immunol 14:1305933","journal-title":"Front Immunol"},{"key":"3033_CR33","doi-asserted-by":"publisher","DOI":"10.3390\/cells15010078","volume":"15","author":"M Tanaka","year":"2026","unstructured":"Tanaka M (2026) Neurogenesis and neuroinflammation in dialogue: mapping gaps, modulating microglia, rewiring aging. Cells 15:78","journal-title":"Cells"},{"key":"3033_CR34","doi-asserted-by":"publisher","first-page":"1428244","DOI":"10.3389\/fnagi.2024.1428244","volume":"16","author":"S Navakkode","year":"2024","unstructured":"Navakkode S, Kennedy BK (2024) Neural ageing and synaptic plasticity: prioritizing brain health in healthy longevity. Front Aging Neurosci 16:1428244","journal-title":"Front Aging Neurosci"},{"key":"3033_CR35","doi-asserted-by":"publisher","first-page":"751","DOI":"10.3233\/JAD-221286","volume":"92","author":"RH Swerdlow","year":"2023","unstructured":"Swerdlow RH (2023) The Alzheimer\u2019s disease mitochondrial cascade hypothesis: a current overview. J Alzheimers Dis 92:751\u2013768","journal-title":"J Alzheimers Dis"},{"key":"3033_CR36","doi-asserted-by":"publisher","first-page":"9","DOI":"10.32598\/rmm.12.1.1322.3","volume":"12","author":"M Zangi Darestani","year":"2024","unstructured":"Zangi Darestani M, Ziaastani Z, Abbasnejad M, Kalantari-Khandani B, Kazemipour A (2024) Identification of critical genes and underlying diseases in early-onset Alzheimer\u2019s disease by bioinformatics approach. Res Mol Med 12:9\u201322","journal-title":"Res Mol Med"},{"key":"3033_CR37","doi-asserted-by":"publisher","DOI":"10.3390\/nu16152399","volume":"16","author":"P Bontempo","year":"2024","unstructured":"Bontempo P, Capasso L, De Masi L, Nebbioso A, Rigano D (2024) Therapeutic potential of natural compounds acting through epigenetic mechanisms in cardiovascular diseases: current findings and future directions. Nutrients 16:2399","journal-title":"Nutrients"},{"key":"3033_CR38","doi-asserted-by":"publisher","first-page":"69","DOI":"10.1038\/s41392-023-01341-7","volume":"8","author":"N Wang","year":"2023","unstructured":"Wang N, Ma T, Yu B (2023) Targeting epigenetic regulators to overcome drug resistance in cancers. Signal Transduct Target Ther 8:69","journal-title":"Signal Transduct Target Ther"},{"key":"3033_CR39","doi-asserted-by":"publisher","first-page":"26","DOI":"10.1177\/0271678X221135061","volume":"43","author":"TS McDonald","year":"2023","unstructured":"McDonald TS, Lerskiatiphanich T, Woodruff TM, McCombe PA, Lee JD (2023) Potential mechanisms to modify impaired glucose metabolism in neurodegenerative disorders. J Cereb Blood Flow Metab 43:26\u201343","journal-title":"J Cereb Blood Flow Metab"},{"key":"3033_CR40","doi-asserted-by":"publisher","DOI":"10.3389\/fnagi.2023.1281338","volume":"15","author":"H Barmaki","year":"2023","unstructured":"Barmaki H, Nourazarian A, Khaki-Khatibi F (2023) Proteostasis and neurodegeneration: a closer look at autophagy in Alzheimer\u2019s disease. Front Aging Neurosci 15:1281338","journal-title":"Front Aging Neurosci"},{"key":"3033_CR41","doi-asserted-by":"publisher","DOI":"10.1038\/s41576-024-00808-9","author":"RL Collins","year":"2025","unstructured":"Collins RL, Talkowski ME (2025) Diversity and consequences of structural variation in the human genome. Nat Rev Genet. https:\/\/doi.org\/10.1038\/s41576-024-00808-9","journal-title":"Nat Rev Genet"},{"key":"3033_CR42","doi-asserted-by":"publisher","first-page":"3143","DOI":"10.1111\/epi.17780","volume":"64","author":"KM Johannesen","year":"2023","unstructured":"Johannesen KM, T\u00fcmer Z, Weckhuysen S, Barakat TS, Bayat A (2023) Solving the unsolved genetic epilepsies: current and future perspectives. Epilepsia 64:3143\u20133154","journal-title":"Epilepsia"},{"key":"3033_CR43","doi-asserted-by":"publisher","first-page":"4067","DOI":"10.3390\/ijms26094067","volume":"26","author":"S Haider","year":"2025","unstructured":"Haider S, Mussolino C (2025) Fine-tuning homology-directed repair (HDR) for precision genome editing: current strategies and future directions. Int J Mol Sci 26:4067","journal-title":"Int J Mol Sci"},{"key":"3033_CR44","doi-asserted-by":"crossref","unstructured":"Zhao Y, Li X, Liu C, Jiang C, Guo X, Xu Q et al (2024) Improving the Efficiency of CRISPR Ribonucleoprotein-Mediated Precise Gene Editing by Small Molecules in Porcine Fibroblasts, Animals, 14:719","DOI":"10.3390\/ani14050719"},{"key":"3033_CR45","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-024-01750-2","volume":"9","author":"Y Zheng","year":"2024","unstructured":"Zheng Y, Li Y, Zhou K, Li T, VanDusen NJ, Hua Y (2024) Precise genome-editing in human diseases: mechanisms, strategies and applications. Signal Transduct Target Ther 9:47","journal-title":"Signal Transduct Target Ther"},{"key":"3033_CR46","doi-asserted-by":"publisher","first-page":"1367","DOI":"10.1093\/neuonc\/noae072","volume":"26","author":"R Rahman","year":"2024","unstructured":"Rahman R, Shi DD, Reitman ZJ, Hamerlik P, De Groot JF, Haas-Kogan DA et al (2024) DNA damage response in brain tumors: a Society for Neuro-Oncology consensus review on mechanisms and translational efforts in neuro-oncology. Neuro Oncol 26:1367\u20131387","journal-title":"Neuro Oncol"},{"key":"3033_CR47","doi-asserted-by":"crossref","unstructured":"Chauhan P, Tiwari I, Sharma NK (2025) Challenges and Opportunities Used in RNA-Based Therapeutics,. RNA-Based Cancer Therap: Techn, Appl Chall Prog,\u00a0pp 231\u2013264","DOI":"10.1007\/978-981-96-3456-9_10"},{"key":"3033_CR48","doi-asserted-by":"publisher","first-page":"663","DOI":"10.1038\/s41587-022-01532-7","volume":"41","author":"L Chen","year":"2023","unstructured":"Chen L, Zhu B, Ru G, Meng H, Yan Y, Hong M et al (2023) Re-engineering the adenine deaminase TadA-8e for efficient and specific CRISPR-based cytosine base editing. Nat Biotechnol 41:663\u2013672","journal-title":"Nat Biotechnol"},{"key":"3033_CR49","doi-asserted-by":"crossref","unstructured":"Wu Y, Xiao Y-L, Tang W (2025) High-precision cytosine base editors by evolving nucleic-acid-recognition hotspots in deaminase. Nat Biotechnol pp 1\u201315","DOI":"10.1038\/s41587-025-02678-w"},{"key":"3033_CR50","doi-asserted-by":"publisher","first-page":"108515","DOI":"10.1016\/j.mrrev.2024.108515","volume":"794","author":"D Wang","year":"2024","unstructured":"Wang D, Zhang Y, Zhang J, Zhao J (2024) Advances in base editing: A focus on base transversions. Mutat Research-Reviews Mutat Res 794:108515","journal-title":"Mutat Research-Reviews Mutat Res"},{"key":"3033_CR51","doi-asserted-by":"publisher","DOI":"10.3389\/fgeed.2023.1068637","volume":"5","author":"A Shakirova","year":"2023","unstructured":"Shakirova A, Karpov T, Komarova Y, Lepik K (2023) In search of an ideal template for therapeutic genome editing: a review of current developments for structure optimization. Frontiers in genome editing 5:1068637","journal-title":"Frontiers in genome editing"},{"key":"3033_CR52","doi-asserted-by":"publisher","first-page":"649","DOI":"10.1089\/hum.2024.043","volume":"35","author":"SY Hosseini","year":"2024","unstructured":"Hosseini SY, Mallick R, M\u00e4kinen P, Yl\u00e4-Herttuala S (2024) Insights into prime editing technology: a deep dive into fundamentals, potentials, and challenges. Hum Gene Ther 35:649\u2013668","journal-title":"Hum Gene Ther"},{"key":"3033_CR53","doi-asserted-by":"publisher","first-page":"161","DOI":"10.1038\/s41576-022-00541-1","volume":"24","author":"PJ Chen","year":"2023","unstructured":"Chen PJ, Liu DR (2023) Prime editing for precise and highly versatile genome manipulation. Nat Rev Genet 24:161\u2013177","journal-title":"Nat Rev Genet"},{"key":"3033_CR54","doi-asserted-by":"publisher","first-page":"3009","DOI":"10.1039\/D3NA01125A","volume":"6","author":"Z Gao","year":"2024","unstructured":"Gao Z (2024) Strategies for enhanced gene delivery to the central nervous system. Nanoscale Adv 6:3009\u20133028","journal-title":"Nanoscale Adv"},{"key":"3033_CR55","doi-asserted-by":"crossref","unstructured":"Sahu B, Behera B, Kumar U (2025) Transgene delivery system: viral, nonviral, and other methods for central nervous system. Genome Editing for Neurodegenerative Diseases. ed: Elsevier, pp 135\u2013155","DOI":"10.1016\/B978-0-443-23826-0.00011-8"},{"key":"3033_CR56","doi-asserted-by":"publisher","DOI":"10.1111\/cpr.13808","volume":"58","author":"M Li","year":"2025","unstructured":"Li M, Lin Y, Cheng Q, Wei T (2025) Prime Editing: A Revolutionary Technology for Precise Treatment of Genetic Disorders. Cell Prolif 58:e13808","journal-title":"Cell Prolif"},{"key":"3033_CR57","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-025-56655-7","volume":"16","author":"L Hu","year":"2025","unstructured":"Hu L, Han J, Wang H-D, Cheng Z-H, Lv C-C, Liu D-F et al (2025) A universal and wide-range cytosine base editor via domain-inlaid and fidelity-optimized CRISPR-FrCas9. Nat Commun 16:1260","journal-title":"Nat Commun"},{"key":"3033_CR58","doi-asserted-by":"publisher","DOI":"10.1016\/j.omtm.2025.101461","author":"S-H Lee","year":"2025","unstructured":"Lee S-H, Wu J, Im D, Hwang G-h, Jeong YK, Jiang H et al (2025) Bystander editing by adenine base editors impairs vision restoration in a mouse model of Leber congenital amaurosis. Mol Ther Methods Clin Dev. https:\/\/doi.org\/10.1016\/j.omtm.2025.101461","journal-title":"Mol Ther Methods Clin Dev"},{"key":"3033_CR59","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2024.1364135","volume":"15","author":"F Xu","year":"2024","unstructured":"Xu F, Zheng C, Xu W, Zhang S, Liu S, Chen X et al (2024) Breaking genetic shackles: the advance of base editing in genetic disorder treatment. Front Pharmacol 15:1364135","journal-title":"Front Pharmacol"},{"key":"3033_CR60","doi-asserted-by":"crossref","unstructured":"Sujatha R, Sarlinraj M, Mohanraj S, Subash T (2025) Integration of metaverse and AoT into the healthcare system. Leveraging Metaverse and Analytics of Things (AoT) in Medical Systems. ed: Elsevier, pp 15\u201339","DOI":"10.1016\/B978-0-443-24049-2.00011-X"},{"key":"3033_CR61","doi-asserted-by":"publisher","DOI":"10.1038\/s12276-025-01514-0","author":"GK Varshney","year":"2025","unstructured":"Varshney GK, Burgess SM (2025) CRISPR-based functional genomics tools in vertebrate models. Exp Mol Med. https:\/\/doi.org\/10.1038\/s12276-025-01514-0","journal-title":"Exp Mol Med"},{"key":"3033_CR62","doi-asserted-by":"publisher","first-page":"333","DOI":"10.1038\/s41589-023-01427-x","volume":"20","author":"L Schmidheini","year":"2024","unstructured":"Schmidheini L, Mathis N, Marquart KF, Rothgangl T, Kissling L, B\u00f6ck D et al (2024) Continuous directed evolution of a compact Cj Cas9 variant with broad PAM compatibility. Nat Chem Biol 20:333\u2013343","journal-title":"Nat Chem Biol"},{"key":"3033_CR63","doi-asserted-by":"publisher","DOI":"10.1093\/plphys\/kiaf197","volume":"198","author":"J Forner","year":"2025","unstructured":"Forner J (2025) Genome modification in plant mitochondria. Plant Physiol 198:kiaf197","journal-title":"Plant Physiol"},{"key":"3033_CR64","doi-asserted-by":"crossref","unstructured":"Song B, Bae S (2023) Genome Editing Using CRISPR. Handbook of Chemical Biology of Nucleic Acids. ed: Springer, pp 2511\u20132536","DOI":"10.1007\/978-981-19-9776-1_85"},{"key":"3033_CR65","unstructured":"Laoharawee K (2022) Engineered Immune Cells to Treat Enzymopathies and Cancers. University of Minnesota"},{"key":"3033_CR66","unstructured":"Kurt IC (2022) Engineering CRISPR-Based DNA Transversion Editing Technologies. Harvard University"},{"key":"3033_CR67","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-025-09021-y","author":"RA Silverstein","year":"2025","unstructured":"Silverstein RA, Kim N, Kroell A-S, Walton RT, Delano J, Butcher RM et al (2025) Custom CRISPR\u2014Cas9 PAM variants via scalable engineering and machine learning. Nature. https:\/\/doi.org\/10.1038\/s41586-025-09021-y","journal-title":"Nature"},{"key":"3033_CR68","doi-asserted-by":"publisher","first-page":"5471","DOI":"10.1038\/s41467-024-49233-w","volume":"15","author":"S Acharya","year":"2024","unstructured":"Acharya S, Ansari AH, Kumar Das P, Hirano S, Aich M, Rauthan R et al (2024) PAM-flexible Engineered FnCas9 variants for robust and ultra-precise genome editing and diagnostics. Nat Commun 15:5471","journal-title":"Nat Commun"},{"key":"3033_CR69","doi-asserted-by":"publisher","first-page":"1466","DOI":"10.1016\/j.ymthe.2025.01.042","volume":"33","author":"Y Tachida","year":"2025","unstructured":"Tachida Y, Manian KV, Butcher R, Levy JM, Pendse N, Hennessey E et al (2025) Systematic empirical evaluation of individual base editing targets: validating therapeutic targets in USH2A and comparison of methods. Mol Ther 33:1466\u20131484","journal-title":"Mol Ther"},{"key":"3033_CR70","unstructured":"Liu S (2023) Evaluation of DNA Minicircles for Delivery of Adenine Base Editors Using Activatable Reporter Imaging Systems. The University of Western Ontario (Canada)"},{"key":"3033_CR71","doi-asserted-by":"publisher","first-page":"83","DOI":"10.1038\/s41434-024-00499-1","volume":"32","author":"JB Murray","year":"2025","unstructured":"Murray JB, Harrison PT, Scholefield J (2025) Prime editing: therapeutic advances and mechanistic insights. Gene Ther 32:83\u201392","journal-title":"Gene Ther"},{"key":"3033_CR72","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-33669-z","volume":"13","author":"S Levesque","year":"2022","unstructured":"Levesque S, Mayorga D, Fiset J-P, Goupil C, Duringer A, Loiselle A et al (2022) Marker-free co-selection for successive rounds of prime editing in human cells. Nat Commun 13:5909","journal-title":"Nat Commun"},{"key":"3033_CR73","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2025.05.003","author":"S Zou","year":"2025","unstructured":"Zou S, Sun Y, Tang W (2025) Charting the development and engineering of CRISPR base editors: lessons and inspirations. Cell Chem Biol. https:\/\/doi.org\/10.1016\/j.chembiol.2025.05.003","journal-title":"Cell Chem Biol"},{"key":"3033_CR74","doi-asserted-by":"publisher","first-page":"1635","DOI":"10.1042\/BST20221508","volume":"51","author":"X Chen","year":"2023","unstructured":"Chen X, McAndrew MJ, Lapinaite A (2023) Unlocking the secrets of ABEs: the molecular mechanism behind their specificity. Biochem Soc Trans 51:1635\u20131646","journal-title":"Biochem Soc Trans"},{"key":"3033_CR75","doi-asserted-by":"publisher","DOI":"10.1063\/5.0228874","author":"Y Lee","year":"2025","unstructured":"Lee Y, Kim B, Lee D, Cheon SY, Lim SG, Kim Y et al (2025) In vivo delivery systems for CRISPR genome editing: viral and non-viral carriers. Appl Phys Rev. https:\/\/doi.org\/10.1063\/5.0228874","journal-title":"Appl Phys Rev"},{"key":"3033_CR76","unstructured":"Davis JR (2023) In Vivo Delivery of Precision Genome Editing Agents. Harvard University"},{"key":"3033_CR77","doi-asserted-by":"publisher","DOI":"10.1186\/s12967-024-05570-4","volume":"22","author":"T Li","year":"2024","unstructured":"Li T, Li S, Kang Y, Zhou J, Yi M (2024) Harnessing the evolving CRISPR\/Cas9 for precision oncology. J Transl Med 22:749","journal-title":"J Transl Med"},{"key":"3033_CR78","unstructured":"Ma K (2023) Improving Genetic Diagnostics and Developing Gene Therapies in Rare Muscle Diseases. Yale University"},{"key":"3033_CR79","doi-asserted-by":"crossref","unstructured":"Fiumara M (2024) UNCOVERING UPSIDES AND PITFALLS OF CAS, BASE AND PRIME EDITING IN HEMATOPOIETIC STEM\/PROGENITOR CELLS","DOI":"10.1097\/01.HS9.0000972380.62376.53"},{"key":"3033_CR80","unstructured":"Konishi CT (2025) Neuroediting: Modeling and Correction of Neurological Diseases Using Personalized. CRISPR\/Cas Genome Editing, New York University"},{"key":"3033_CR81","doi-asserted-by":"publisher","DOI":"10.3390\/pharmaceutics15041100","volume":"15","author":"AH Meyer","year":"2023","unstructured":"Meyer AH, Feldsien TM, Mezler M, Untucht C, Venugopalan R, Lefebvre DR (2023) Novel developments to enable treatment of CNS diseases with targeted drug delivery. Pharmaceutics 15:1100","journal-title":"Pharmaceutics"},{"key":"3033_CR82","doi-asserted-by":"publisher","DOI":"10.1039\/D4CS00483C","author":"A Ressnerova","year":"2025","unstructured":"Ressnerova A, Heger Z, Pumera M (2025) Translational nanorobotics breaking through biological membranes. Chem Soc Rev. https:\/\/doi.org\/10.1039\/D4CS00483C","journal-title":"Chem Soc Rev"},{"key":"3033_CR83","doi-asserted-by":"crossref","unstructured":"Gharat SA, Momin MM, Khan T (2024) Clinical Applications of Pharmacokinetic and Pharmacodynamic Studies of Targeted Novel Drug Delivery Systems. Pharmacokinetics and Pharmacodynamics of Novel Drug Delivery Systems: From Basic Concepts to Applications: A Machine-Generated Literature Overview. ed: Springer, pp 275\u2013342","DOI":"10.1007\/978-981-99-7858-8_5"},{"key":"3033_CR84","doi-asserted-by":"publisher","first-page":"fuae020","DOI":"10.1093\/femsre\/fuae020","volume":"48","author":"S Vercauteren","year":"2024","unstructured":"Vercauteren S, Fiesack S, Maroc L, Verstraeten N, Dewachter L, Michiels J et al (2024) The rise and future of CRISPR-based approaches for high-throughput genomics. FEMS Microbiol Rev 48:fuae020","journal-title":"FEMS Microbiol Rev"},{"key":"3033_CR85","doi-asserted-by":"crossref","unstructured":"Stettler M, Hoby S, Wenker C, Wyss F, Heiderich E, Butticaz L et al (2025) Immunization with a novel RNA replicon vaccine confers long-lasting protection against H5N1 avian influenza virus in 24 bird species, bioRxiv, p 2025.01. 15.633174","DOI":"10.1101\/2025.01.15.633174"},{"key":"3033_CR86","doi-asserted-by":"crossref","unstructured":"Yu J, Jin J, Kwon E, Jang H, Choi S-k, Kim D et al (2025) Programmable RNA acetylation with CRISPR\u2013Cas13. Nat Chem Biol, pp 1\u201314","DOI":"10.1038\/s41589-025-01922-3"},{"key":"3033_CR87","doi-asserted-by":"publisher","DOI":"10.1155\/2023\/5525165","volume":"2023","author":"S Bafandeh","year":"2023","unstructured":"Bafandeh S, Khodadadi E, Ganbarov K, Asgharzadeh M, K\u00f6se \u015e, Samadi Kafil H (2023) Natural products as a potential source of promising therapeutics for COVID-19 and viral diseases. Evid Based Complement Alternat Med 2023:5525165","journal-title":"Evid Based Complement Alternat Med"},{"key":"3033_CR88","doi-asserted-by":"publisher","DOI":"10.1016\/j.omtn.2024.102440","author":"S Naeem","year":"2025","unstructured":"Naeem S, Zhang J, Zhang Y, Wang Y (2025) Nucleic acid therapeutics: past, present, and future. Mol Ther Nucleic Acids. https:\/\/doi.org\/10.1016\/j.omtn.2024.102440","journal-title":"Mol Ther Nucleic Acids"},{"key":"3033_CR89","doi-asserted-by":"publisher","first-page":"115","DOI":"10.1038\/s41392-025-02140-y","volume":"10","author":"Y Cai","year":"2025","unstructured":"Cai Y, Chai T, Nguyen W, Liu J, Xiao E, Ran X et al (2025) Phototherapy in cancer treatment: strategies and challenges. Signal Transduct Target Therapy 10:115","journal-title":"Signal Transduct Target Therapy"},{"key":"3033_CR90","doi-asserted-by":"publisher","DOI":"10.3892\/ijmm.2025.5531","volume":"55","author":"AA Aloliqi","year":"2025","unstructured":"Aloliqi AA, Alnuqaydan AM, Albutti A, Alharbi BF, Rahmani AH, Khan AA (2025) Current updates regarding biogenesis, functions and dysregulation of microRNAs in cancer: innovative approaches for detection using CRISPR\/Cas13-based platforms. Int J Mol Med 55:90","journal-title":"Int J Mol Med"},{"key":"3033_CR91","doi-asserted-by":"publisher","first-page":"R47","DOI":"10.1093\/hmg\/ddac193","volume":"31","author":"IR Jones","year":"2022","unstructured":"Jones IR, Ren X, Shen Y (2022) High-throughput CRISPRi and CRISPRa technologies in 3D genome regulation for neuropsychiatric diseases. Hum Mol Genet 31:R47\u2013R53","journal-title":"Hum Mol Genet"},{"key":"3033_CR92","doi-asserted-by":"crossref","unstructured":"Laub S, Tulina N, Hoffman M, Faryean J, Ramachandran S, Trang K et al (2025) Integration of Alzheimer\u2019s GWAS, 3D genomics, and single-cell CRISPRi non-coding screen implicates causal variants in a microglial enhancer regulating TSPAN14, bioRxiv, p. 2025.04. 01.646442","DOI":"10.1101\/2025.04.01.646442"},{"key":"3033_CR93","doi-asserted-by":"publisher","first-page":"73","DOI":"10.1089\/crispr.2023.0078","volume":"7","author":"NN Mohamad Zamberi","year":"2024","unstructured":"Mohamad Zamberi NN, Abuhamad AY, Low TY, Mohtar MA, Syafruddin SE (2024) dCas9 tells tales: probing gene function and transcription regulation in cancer. CRISPR J 7:73\u201387","journal-title":"CRISPR J"},{"key":"3033_CR94","unstructured":"Taifour S (2024) Locus-selective targeting of the oncogenic fusion EWSR1-FLI1 in Ewing sarcoma by CRISPR\/dCas9 epigenetic silencers"},{"key":"3033_CR95","doi-asserted-by":"publisher","first-page":"190","DOI":"10.1038\/s41594-023-01171-9","volume":"31","author":"Y Zhu","year":"2024","unstructured":"Zhu Y, Vvedenskaya IO, Sze S-H, Nickels BE, Kaplan CD (2024) Quantitative analysis of transcription start site selection reveals control by DNA sequence, RNA polymerase II activity and NTP levels. Nat Struct Mol Biol 31:190\u2013202","journal-title":"Nat Struct Mol Biol"},{"key":"3033_CR96","unstructured":"Zhu Y (2022) Determination of DNA sequence, Pol II catalytic activity and TFIIH subunit Tfb3 functions in transcription start site selection in Saccharomyces cerevisiae. University of Pittsburgh"},{"key":"3033_CR97","doi-asserted-by":"publisher","DOI":"10.1016\/j.xplc.2025.101427","author":"J Liu","year":"2025","unstructured":"Liu J, Zhang R, Chai N, Su L, Zheng Z, Liu T et al (2025) Programmable genome engineering and gene modifications for plant biodesign. Plant Commun. https:\/\/doi.org\/10.1016\/j.xplc.2025.101427","journal-title":"Plant Commun"},{"key":"3033_CR98","doi-asserted-by":"crossref","unstructured":"Blum K, Bowirrat A, Baron D, Elman I, Makale MT, Cadet JL et al (2024) Identification of stress-induced epigenetic methylation onto dopamine D2 gene and neurological and behavioral consequences, Gene & protein in disease\u00a029;3(1):10.36922\/gpd. 1966","DOI":"10.36922\/gpd.1966"},{"key":"3033_CR99","doi-asserted-by":"publisher","first-page":"1375","DOI":"10.1111\/pbi.14533","volume":"23","author":"N Gondalia","year":"2025","unstructured":"Gondalia N, Quiroz LF, Lai L, Singh AK, Khan M, Brychkova G et al (2025) Harnessing promoter elements to enhance gene editing in plants: perspectives and advances. Plant Biotechnol J 23:1375\u20131395","journal-title":"Plant Biotechnol J"},{"key":"3033_CR100","doi-asserted-by":"crossref","unstructured":"Shafi N, Manzoor T, Hussain I, Nazir J, Ali A, Gani U (2026) Current Vectors for In Vivo Delivery of CRISPR-Cas9 System. Gene Editing by CRISPR-Cas. pp 258\u2013282","DOI":"10.1201\/9781003537151-11"},{"key":"3033_CR101","doi-asserted-by":"publisher","first-page":"324","DOI":"10.3390\/jfb15110324","volume":"15","author":"B Kaupbayeva","year":"2024","unstructured":"Kaupbayeva B, Tsoy A, Safarova Y, Nurmagambetova A, Murata H, Matyjaszewski K et al (2024) Unlocking genome editing: Advances and obstacles in CRISPR\/Cas delivery technologies. J Funct Biomaterials 15:324","journal-title":"J Funct Biomaterials"},{"key":"3033_CR102","doi-asserted-by":"publisher","DOI":"10.3390\/ijms26136371","volume":"26","author":"MA Kovalev","year":"2025","unstructured":"Kovalev MA, Mamaeva NY, Kristovskiy NV, Feskin PG, Vinnikov RS, Oleinikov PD et al (2025) Epigenome engineering using dCas systems for biomedical applications and biotechnology: current achievements, opportunities and challenges. Int J Mol Sci 26:6371","journal-title":"Int J Mol Sci"},{"key":"3033_CR103","unstructured":"Genga RM (2019) Towards Understanding the Molecular Basis of Human Endoderm Development Using CRISPR-Effector and Single-Cell Technologies"},{"key":"3033_CR104","unstructured":"Aulston BD, Gimse K, Bazick HO, Kramar EA, Pizzo DP, Parra-Rivas LA et al (2025) Long term rescue of Alzheimer\u2019s deficits in vivo by one-time gene-editing of App C-terminus, bioRxiv, p 2024.06.08.598099"},{"key":"3033_CR105","doi-asserted-by":"publisher","DOI":"10.1186\/s40035-024-00415-y","volume":"13","author":"MS Gee","year":"2024","unstructured":"Gee MS, Kwon E, Song M-H, Jeon SH, Kim N, Lee JK et al (2024) CRISPR base editing-mediated correction of a tau mutation rescues cognitive decline in a mouse model of tauopathy. Transl Neurodegener 13:21","journal-title":"Transl Neurodegener"},{"key":"3033_CR106","doi-asserted-by":"publisher","first-page":"7028","DOI":"10.1038\/s41467-024-51163-6","volume":"15","author":"ND Schartz","year":"2024","unstructured":"Schartz ND, Liang HY, Carvalho K, Chu S-H, Mendoza-Arvilla A, Petrisko TJ et al (2024) C5aR1 antagonism suppresses inflammatory glial responses and alters cellular signaling in an Alzheimer\u2019s disease mouse model. Nat Commun 15:7028","journal-title":"Nat Commun"},{"key":"3033_CR107","doi-asserted-by":"publisher","first-page":"66","DOI":"10.1177\/0271678X231159597","volume":"43","author":"JM Basak","year":"2023","unstructured":"Basak JM, Falk M, Mitchell DN, Coakley KA, Quillinan N, Orfila JE et al (2023) Targeting BACE1-mediated production of amyloid beta improves hippocampal synaptic function in an experimental model of ischemic stroke. J Cereb Blood Flow Metab 43:66\u201377","journal-title":"J Cereb Blood Flow Metab"},{"key":"3033_CR108","doi-asserted-by":"publisher","first-page":"2470","DOI":"10.1007\/s12035-023-03217-x","volume":"60","author":"X Peng","year":"2023","unstructured":"Peng X, Guo H, Zhang X, Yang Z, Ruganzu JB, Yang Z et al (2023) TREM2 inhibits tau hyperphosphorylation and neuronal apoptosis via the PI3K\/Akt\/GSK-3\u03b2 signaling pathway in vivo and in vitro. Mol Neurobiol 60:2470\u20132485","journal-title":"Mol Neurobiol"},{"key":"3033_CR109","doi-asserted-by":"crossref","unstructured":"Koya PC, Kolla SC, Madala V, Sayana SB (2025) Potential of Nerve Growth Factor (NGF)-and Brain-Derived Neurotrophic Factor (BDNF)-Targeted Gene Therapy for Alzheimer\u2019s Disease: A Narrative Review, Cureus, vol 17","DOI":"10.7759\/cureus.85814"},{"key":"3033_CR110","doi-asserted-by":"publisher","first-page":"962769","DOI":"10.3389\/fphys.2022.962769","volume":"13","author":"M Watroba","year":"2022","unstructured":"Watroba M, Szukiewicz D (2022) Sirtuins promote brain homeostasis, preventing Alzheimer\u2019s disease through targeting neuroinflammation. Front Physiol 13:962769","journal-title":"Front Physiol"},{"key":"3033_CR111","doi-asserted-by":"publisher","first-page":"117172","DOI":"10.1016\/j.jep.2023.117172","volume":"319","author":"Z Zhao","year":"2024","unstructured":"Zhao Z, Xie L, Shi J, Liu T, Wang S, Huang J et al (2024) Neuroprotective effect of Zishen Huoxue decoction treatment on vascular dementia by activating PINK1\/Parkin mediated Mitophagy in the hippocampal CA1 region. J Ethnopharmacol 319:117172","journal-title":"J Ethnopharmacol"},{"key":"3033_CR112","doi-asserted-by":"publisher","first-page":"429","DOI":"10.1016\/j.omtn.2018.03.007","volume":"11","author":"B Gy\u00f6rgy","year":"2018","unstructured":"Gy\u00f6rgy B, L\u00f6\u00f6v C, Zaborowski MP, Takeda S, Kleinstiver BP, Commins C et al (2018) CRISPR\/Cas9 mediated disruption of the Swedish APP allele as a therapeutic approach for early-onset Alzheimer\u2019s disease. Mol Ther Nucleic Acids 11:429\u2013440","journal-title":"Mol Ther Nucleic Acids"},{"key":"3033_CR113","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-07971-8","volume":"10","author":"J Sun","year":"2019","unstructured":"Sun J, Carlson-Stevermer J, Das U, Shen M, Delenclos M, Snead AM et al (2019) CRISPR\/Cas9 editing of APP C-terminus attenuates \u03b2-cleavage and promotes \u03b1-cleavage. Nat Commun 10:53","journal-title":"Nat Commun"},{"key":"3033_CR114","unstructured":"Kwart D (2019) Modeling Alzheimer\u2019s Disease Using CRISPR\/CAS9 Gene Editing and Induced. Pluripotent Stem Cells Reveals Conserved Cellular Mechanisms"},{"key":"3033_CR115","doi-asserted-by":"publisher","DOI":"10.3390\/antiox14070781","volume":"14","author":"I Raffaele","year":"2025","unstructured":"Raffaele I, Cipriano GL, Anchesi I, Oddo S, Silvestro S (2025) CRISPR\/Cas9 and iPSC-based therapeutic approaches in Alzheimer\u2019s disease. Antioxidants 14:781","journal-title":"Antioxidants"},{"key":"3033_CR116","doi-asserted-by":"publisher","first-page":"4147","DOI":"10.3390\/cimb46050255","volume":"46","author":"E Deneault","year":"2024","unstructured":"Deneault E (2024) Recent therapeutic gene editing applications to genetic disorders. Curr Issues Mol Biol 46:4147\u20134185","journal-title":"Curr Issues Mol Biol"},{"key":"3033_CR117","doi-asserted-by":"crossref","unstructured":"Gonz\u00e1lez Molina LA, Dolga AM, Rots MG, Sarno F (2025) The promise of epigenetic editing for treating brain disorders. Neuroepigenetics Mechanisms in Health and Disease. pp 111\u2013190","DOI":"10.1007\/978-3-031-75980-2_4"},{"key":"3033_CR118","doi-asserted-by":"publisher","first-page":"24","DOI":"10.1089\/humc.2017.231","volume":"29","author":"JB Rosenberg","year":"2018","unstructured":"Rosenberg JB, Kaplitt MG, De BP, Chen A, Flagiello T, Salami C et al (2018) AAVrh. 10-mediated APOE2 central nervous system gene therapy for APOE4-associated Alzheimer\u2019s disease. Hum Gene Ther Clin Dev 29:24\u201347","journal-title":"Hum Gene Ther Clin Dev"},{"key":"3033_CR119","doi-asserted-by":"crossref","unstructured":"Carvalho K, Schartz ND, Balderrama-Gutierrez G, Liang HY, Chu S-H, Selvan P et al (2022) Modulation of C5a\u2013C5aR1 signaling alters the dynamics of AD progression. J Neuroinflammation 19:178","DOI":"10.1186\/s12974-022-02539-2"},{"key":"3033_CR120","doi-asserted-by":"crossref","unstructured":"Yang X, Jones IR, Chen PB, Yang H, Ren X, Zheng L et al (2023) Functional characterization of gene regulatory elements and neuropsychiatric disease-associated risk loci in iPSCs and iPSC-derived neurons, bioRxiv, p 2023.08.30.555359","DOI":"10.1101\/2023.08.30.555359"},{"key":"3033_CR121","unstructured":"Martino RA (2022) Characterization of the AAV-PHP. B Capsid and Its Interaction with the Ly6a Receptor. Implications for Receptor-Targeted Vector Engineering, University of Pennsylvania"},{"key":"3033_CR122","doi-asserted-by":"publisher","first-page":"3439","DOI":"10.1021\/acsptsci.4c00068","volume":"7","author":"S Risen","year":"2024","unstructured":"Risen S, Sharma S, Gilberto VS, Brindley S, Aguilar M, Brown JM et al (2024) Large-and small-animal studies of safety, pharmacokinetics, and biodistribution of inflammasome-targeting nanoligomer in the brain and other target organs. ACS Pharmacol Translational Sci 7:3439\u20133451","journal-title":"ACS Pharmacol Translational Sci"},{"key":"3033_CR123","doi-asserted-by":"publisher","DOI":"10.3389\/fnmol.2023.1248271","volume":"16","author":"EA Salegio","year":"2023","unstructured":"Salegio EA, Hancock K, Korszen S (2023) Pre-clinical delivery of gene therapy products to the cerebrospinal fluid: challenges and considerations for clinical translation. Front Mol Neurosci 16:1248271","journal-title":"Front Mol Neurosci"},{"key":"3033_CR124","unstructured":"Lawrence J (2024) Role of Glia in Diurnal Rhythms in Neuroinflammation and Blood-Brain Barrier Breakdown. Washington University in St. Louis"},{"key":"3033_CR125","doi-asserted-by":"publisher","first-page":"578","DOI":"10.3390\/ijms26020578","volume":"26","author":"N Bara-Ledesma","year":"2025","unstructured":"Bara-Ledesma N, Viteri-Noel A, Lopez Rodriguez M, Stamatakis K, Fabregate M, Vazquez-Santos A et al (2025) Advances in gene therapy for rare diseases: targeting functional haploinsufficiency through AAV and mRNA approaches. Int J Mol Sci 26:578","journal-title":"Int J Mol Sci"},{"key":"3033_CR126","doi-asserted-by":"publisher","DOI":"10.3390\/v16030442","volume":"16","author":"E Lopez-Gordo","year":"2024","unstructured":"Lopez-Gordo E, Chamberlain K, Riyad JM, Kohlbrenner E, Weber T (2024) Natural adeno-associated virus serotypes and engineered adeno-associated virus capsid variants: tropism differences and mechanistic insights. Viruses 16:442","journal-title":"Viruses"},{"key":"3033_CR127","doi-asserted-by":"publisher","first-page":"425","DOI":"10.1007\/s12033-024-01070-4","volume":"67","author":"O Volodina","year":"2025","unstructured":"Volodina O, Smirnikhina S (2025) The future of gene therapy: a review of in vivo and ex vivo delivery methods for genome editing-based therapies. Mol Biotechnol 67:425\u2013437","journal-title":"Mol Biotechnol"},{"key":"3033_CR128","unstructured":"Berdeckaa D, De Smedta SC, De Vosb WH, Braeckmansa K (2023) Non-viral delivery of RNA for therapeutic T cell engineering ex vivo, Towards minimally invasive delivery of large molecules into human T cells by photoporation with polydopamine nanosensitizers and photothermal nanofibers, vol 9"},{"key":"3033_CR129","doi-asserted-by":"publisher","DOI":"10.1016\/j.cjprs.2025.05.001","author":"H Chen","year":"2025","unstructured":"Chen H, Li Q (2025) Recent advances in scalable exosome production: challenges and innovations. Zhonghua Zheng Xing Wai Ke Za Zhi. https:\/\/doi.org\/10.1016\/j.cjprs.2025.05.001","journal-title":"Zhonghua Zheng Xing Wai Ke Za Zhi"},{"key":"3033_CR130","doi-asserted-by":"publisher","DOI":"10.1016\/j.bas.2025.104193","volume":"5","author":"G Carone","year":"2025","unstructured":"Carone G, Bonada M, Belotti EG, D\u2019Angeli E, Piccardi A, Doniselli FM et al (2025) Post-craniotomy infections: a point-by-point approach. Brain Spine 5:104193","journal-title":"Brain Spine"},{"key":"3033_CR131","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24043866","volume":"24","author":"Y El Ouaamari","year":"2023","unstructured":"El Ouaamari Y, den Van Bos J, Willekens B, Cools N, Wens I (2023) Neurotrophic factors as regenerative therapy for neurodegenerative diseases: current status, challenges and future perspectives. Int J Mol Sci 24:3866","journal-title":"Int J Mol Sci"},{"key":"3033_CR132","doi-asserted-by":"publisher","first-page":"898","DOI":"10.1038\/s41573-024-01051-x","volume":"23","author":"PJ Carter","year":"2024","unstructured":"Carter PJ, Quarmby V (2024) Immunogenicity risk assessment and mitigation for engineered antibody and protein therapeutics. Nat Rev Drug Discov 23:898\u2013913","journal-title":"Nat Rev Drug Discov"},{"key":"3033_CR133","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2025.04.006","author":"P Prasanna","year":"2025","unstructured":"Prasanna P, Herrera B, Schlesinger LS, Paiardini M, Sharan R (2025) Advances in host-directed therapy for tuberculosis and HIV coinfection: enhancing immune responses. Trends Microbiol. https:\/\/doi.org\/10.1016\/j.tim.2025.04.006","journal-title":"Trends Microbiol"},{"key":"3033_CR134","doi-asserted-by":"publisher","DOI":"10.3390\/cancers16010236","volume":"16","author":"M Pinkiewicz","year":"2024","unstructured":"Pinkiewicz M, Pinkiewicz M, Walecki J, Zaczy\u0144ski A, Zawadzki M (2024) Breaking barriers in neuro-oncology: a scoping literature review on invasive and non-invasive techniques for blood\u2013brain barrier disruption. Cancers (Basel) 16:236","journal-title":"Cancers (Basel)"},{"key":"3033_CR135","doi-asserted-by":"publisher","first-page":"1541872","DOI":"10.3389\/fphar.2025.1541872","volume":"16","author":"P Buchwald","year":"2025","unstructured":"Buchwald P (2025) Quantification of signal amplification for receptors: the K d\/EC50 ratio of full agonists as a gain parameter. Front Pharmacol 16:1541872","journal-title":"Front Pharmacol"},{"key":"3033_CR136","doi-asserted-by":"publisher","first-page":"1022","DOI":"10.1016\/j.gendis.2023.02.051","volume":"11","author":"Y Zhang","year":"2024","unstructured":"Zhang Y, Xie X, Chen B, Pan L, Li J, Wang W et al (2024) E674Q (Shanghai APP mutant), a novel amyloid precursor protein mutation, in familial late-onset Alzheimer\u2019s disease. Genes Dis 11:1022\u20131034","journal-title":"Genes Dis"},{"key":"3033_CR137","doi-asserted-by":"publisher","first-page":"48","DOI":"10.1186\/s13024-025-00832-1","volume":"20","author":"SG Fern\u00e1ndez","year":"2025","unstructured":"Fern\u00e1ndez SG, Oria CG, Petit D, Annaert W, Ringman JM, Fox NC et al (2025) Spectrum of \u03b3-Secretase dysfunction as a unifying predictor of ADAD age at onset across PSEN1, PSEN2 and APP causal genes. Mol Neurodegeneration 20:48","journal-title":"Mol Neurodegeneration"},{"key":"3033_CR138","doi-asserted-by":"publisher","first-page":"2009","DOI":"10.1038\/s41380-024-02463-2","volume":"29","author":"B Ng","year":"2024","unstructured":"Ng B, Vowles J, Bertherat F, Abey A, Kilfeather P, Beccano-Kelly D et al (2024) Tau depletion in human neurons mitigates A\u03b2-driven toxicity. Mol Psychiatry 29:2009\u20132020","journal-title":"Mol Psychiatry"},{"key":"3033_CR139","doi-asserted-by":"publisher","first-page":"1004111","DOI":"10.37349\/ent.2025.1004111","volume":"5","author":"S Pal","year":"2025","unstructured":"Pal S, Mandal S (2025) Therapeutic potential of microRNAs in neurological disorders: mechanisms, biomarkers, and emerging therapeutic strategies. Exploration of Neuroprotective Therapy 5:1004111","journal-title":"Exploration of Neuroprotective Therapy"},{"key":"3033_CR140","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1007\/s40291-024-00738-6","volume":"29","author":"MC Tartaglia","year":"2025","unstructured":"Tartaglia MC, Ingelsson M (2025) Molecular therapeutics in development to treat Alzheimer\u2019s disease. Mol Diagn Ther 29:9\u201324","journal-title":"Mol Diagn Ther"},{"key":"3033_CR141","doi-asserted-by":"crossref","unstructured":"Hagena H, Manahan-Vaughan D (2024) Interplay of hippocampal long-term potentiation and long-term depression in enabling memory representations. Philos Trans R Soc Lond B Biol Sci 379:20230229","DOI":"10.1098\/rstb.2023.0229"},{"key":"3033_CR142","doi-asserted-by":"publisher","first-page":"126","DOI":"10.1038\/s41398-025-03358-8","volume":"15","author":"Y Zou","year":"2025","unstructured":"Zou Y, Yang L, Zhu J, Fan J, Zheng H, Liao X et al (2025) Pitolisant alleviates brain network dysfunction and cognitive deficits in a mouse model of Alzheimer\u2019s disease. Translational Psychiatry 15:126","journal-title":"Translational Psychiatry"},{"key":"3033_CR143","doi-asserted-by":"publisher","first-page":"4960","DOI":"10.3390\/ijms24054960","volume":"24","author":"C Yin","year":"2023","unstructured":"Yin C, Harms AC, Hankemeier T, Kindt A, de Lange EC (2023) Status of metabolomic measurement for insights in Alzheimer\u2019s disease progression\u2014what is missing? Int J Mol Sci 24:4960","journal-title":"Int J Mol Sci"},{"key":"3033_CR144","doi-asserted-by":"publisher","first-page":"613","DOI":"10.1038\/s44318-024-00352-6","volume":"44","author":"Y Li","year":"2025","unstructured":"Li Y, Sun S (2025) RNA dysregulation in neurodegenerative diseases. EMBO J 44:613\u2013638","journal-title":"EMBO J"},{"key":"3033_CR145","doi-asserted-by":"publisher","DOI":"10.1002\/alz.70025","volume":"21","author":"B Aguzzoli Heberle","year":"2025","unstructured":"Aguzzoli Heberle B, Fox KL, Lobraico Libermann L, Ronchetti Martins Xavier S, Tarnowski Dallarosa G, Carolina Santos R et al (2025) Systematic review and meta-analysis of bulk RNAseq studies in human Alzheimer\u2019s disease brain tissue. Alzheimers Dement 21:e70025","journal-title":"Alzheimers Dement"},{"key":"3033_CR146","doi-asserted-by":"publisher","DOI":"10.3390\/ijms26157271","volume":"26","author":"Y Yu","year":"2025","unstructured":"Yu Y, Wang C, Wang B, Wang X, Zhao Q, Yan Y et al (2025) Clusterin regulates the mechanisms of neuroinflammation and neuronal circuit impairment in Alzheimer\u2019s Disease. Int J Mol Sci 26:7271","journal-title":"Int J Mol Sci"},{"key":"3033_CR147","doi-asserted-by":"publisher","first-page":"2","DOI":"10.1007\/s12017-024-08815-z","volume":"27","author":"X Zhuang","year":"2024","unstructured":"Zhuang X, Lin J, Song Y, Ban R, Zhao X, Xia Z et al (2024) The interplay between accumulation of Amyloid-Beta and Tau proteins, PANoptosis, and inflammation in Alzheimer\u2019s disease. Neuromol Med 27:2","journal-title":"Neuromol Med"},{"key":"3033_CR148","doi-asserted-by":"publisher","DOI":"10.3390\/diagnostics15020211","volume":"15","author":"M Sarma","year":"2025","unstructured":"Sarma M, Chatterjee S (2025) Machine learning-based Alzheimer\u2019s disease stage diagnosis utilizing blood gene expression and clinical data: a comparative investigation. Diagnostics (Basel) 15:211","journal-title":"Diagnostics (Basel)"},{"key":"3033_CR149","doi-asserted-by":"publisher","first-page":"59","DOI":"10.1146\/annurev-biodatasci-102523-103838","volume":"7","author":"EM McDonagh","year":"2024","unstructured":"McDonagh EM, Trynka G, McCarthy M, Holzinger ER, Khader S, Nakic N et al (2024) Human genetics and genomics for drug target identification and prioritization: open Targets\u2019 perspective. Annu Rev Biomed Data Sci 7:59\u201381","journal-title":"Annu Rev Biomed Data Sci"},{"key":"3033_CR150","doi-asserted-by":"publisher","first-page":"1553667","DOI":"10.3389\/fmolb.2025.1553667","volume":"12","author":"AA Kattuparambil","year":"2025","unstructured":"Kattuparambil AA, Chaurasia DK, Shekhar S, Srinivasan A, Mondal S, Aduri R et al (2025) Exploring chemical space for druglike small molecules in the age of AI. Front Mol Biosci 12:1553667","journal-title":"Front Mol Biosci"},{"key":"3033_CR151","doi-asserted-by":"publisher","DOI":"10.1098\/rsob.210280","volume":"12","author":"LA Jonas","year":"2022","unstructured":"Jonas LA, Jain T, Li Y-M (2022) Functional insight into LOAD-associated microglial response genes. Open Biol 12:210280","journal-title":"Open Biol"},{"key":"3033_CR152","doi-asserted-by":"publisher","DOI":"10.2147\/IJN.S455574","author":"KS Allemailem","year":"2024","unstructured":"Allemailem KS, Almatroudi A, Rahmani AH, Alrumaihi F, Alradhi AE, Alsubaiyel AM (2024) Recent updates of the CRISPR\/Cas9 genome editing system: novel approaches to regulate its spatiotemporal control by genetic and physicochemical strategies. Int J Nanomedicine. https:\/\/doi.org\/10.2147\/IJN.S455574","journal-title":"Int J Nanomedicine"},{"key":"3033_CR153","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2023.1335901","volume":"11","author":"S Dixit","year":"2024","unstructured":"Dixit S, Kumar A, Srinivasan K, Vincent PDR, Krishnan NR (2024) Advancing genome editing with artificial intelligence: opportunities, challenges, and future directions. Frontiers in bioengineering and biotechnology 11:1335901","journal-title":"Frontiers in bioengineering and biotechnology"},{"key":"3033_CR154","doi-asserted-by":"crossref","unstructured":"Zafar I, Rafique A, Fazal J, Manzoor M, Ain QU, Rayan RA (2021) Genome and gene editing by artificial intelligence programs. Advanced AI techniques and applications in bioinformatics. ed: CRC, pp 165\u2013188","DOI":"10.1201\/9781003126164-8"},{"key":"3033_CR155","doi-asserted-by":"crossref","unstructured":"Rayan RA, Zafar I, Tsagkaris C (2021) Deep learning for health and medicine. Deep learning for personalized healthcare services, vol 1","DOI":"10.1515\/9783110708127-001"},{"key":"3033_CR156","doi-asserted-by":"publisher","first-page":"140","DOI":"10.1038\/s41588-024-02039-4","volume":"57","author":"JS Funk","year":"2025","unstructured":"Funk JS, Klimovich M, Drangenstein D, Pielhoop O, Hunold P, Borowek A et al (2025) Deep CRISPR mutagenesis characterizes the functional diversity of TP53 mutations. Nat Genet 57:140\u2013153","journal-title":"Nat Genet"},{"key":"3033_CR157","doi-asserted-by":"crossref","unstructured":"Khammampalli S, Vindal V (2025) Artificial Intelligence in CRISPR-Cas Systems: A Review of Tool Applications. Artificial Intelligence (AI) in Cell and Genetic Engineering. pp 243\u2013257","DOI":"10.1007\/978-1-0716-4690-8_14"},{"key":"3033_CR158","doi-asserted-by":"publisher","DOI":"10.1002\/jgm.70061","volume":"27","author":"J Patel","year":"2025","unstructured":"Patel J, Patel D, Raval A (2025) Artificial intelligence for predictive modeling in CRISPR\/Cas9 gene editing: a survey of methods and design strategies. J Gene Med 27:e70061","journal-title":"J Gene Med"},{"key":"3033_CR159","doi-asserted-by":"publisher","DOI":"10.3389\/fgeed.2025.1612868","volume":"7","author":"C He","year":"2025","unstructured":"He C, Chen B, Yan C, Zhou X (2025) Stem cell and CRISPR\/Cas9 gene editing technology in Alzheimer\u2019s disease therapy: from basic research to clinical innovation. Frontiers in genome editing 7:1612868","journal-title":"Frontiers in genome editing"},{"key":"3033_CR160","doi-asserted-by":"publisher","DOI":"10.3390\/ijms252212311","volume":"25","author":"MG Sighencea","year":"2024","unstructured":"Sighencea MG, Popescu R\u0218, Trifu SC (2024) From fundamentals to innovation in Alzheimer\u2019s disease: molecular findings and revolutionary therapies. Int J Mol Sci 25:12311","journal-title":"Int J Mol Sci"},{"key":"3033_CR161","doi-asserted-by":"publisher","first-page":"340","DOI":"10.1111\/bph.17302","volume":"182","author":"Z Bereczki","year":"2025","unstructured":"Bereczki Z, Benczik B, Balogh OM, Marton S, Puhl E, P\u00e9terv\u00e1ri M et al (2025) Mitigating off-target effects of small RNAs: conventional approaches, network theory and artificial intelligence. Br J Pharmacol 182:340\u2013379","journal-title":"Br J Pharmacol"},{"key":"3033_CR162","doi-asserted-by":"publisher","DOI":"10.1002\/wrna.1694","volume":"13","author":"M Kordy\u015b","year":"2022","unstructured":"Kordy\u015b M, Sen R, Warkocki Z (2022) Applications of the versatile CRISPR-Cas13 RNA targeting system. Wiley Interdisciplinary Reviews: RNA 13:e1694","journal-title":"Wiley Interdisciplinary Reviews: RNA"},{"key":"3033_CR163","doi-asserted-by":"publisher","DOI":"10.3390\/genes16101168","volume":"16","author":"MA Boti","year":"2025","unstructured":"Boti MA, Diamantopoulos MA, Scorilas A (2025) RNA-targeting techniques: a comparative analysis of modern approaches for RNA manipulation in cancer research and therapeutics. Genes 16:1168","journal-title":"Genes"},{"key":"3033_CR164","doi-asserted-by":"crossref","unstructured":"Johansson C (2025) The Alzheimer disease continuum: findings from monogenic Alzheimer disease. Karolinska Institutet","DOI":"10.69622\/28639856.v1"},{"key":"3033_CR165","doi-asserted-by":"publisher","first-page":"169","DOI":"10.1080\/1061186X.2025.2544786","volume":"34","author":"J Dong","year":"2026","unstructured":"Dong J, Sun M, Li Y, Xie Z (2026) Multi-omics approaches in gene therapy for vascular diseases: bridging genomics, transcriptomics, and epigenetics. J Drug Target 34:169\u2013184","journal-title":"J Drug Target"}],"container-title":["Acta Neurologica Belgica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s13760-026-03033-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s13760-026-03033-9","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s13760-026-03033-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T18:02:26Z","timestamp":1777658546000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s13760-026-03033-9"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,3]]},"references-count":165,"alternative-id":["3033"],"URL":"https:\/\/doi.org\/10.1007\/s13760-026-03033-9","relation":{},"ISSN":["0300-9009","2240-2993"],"issn-type":[{"value":"0300-9009","type":"print"},{"value":"2240-2993","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,3]]},"assertion":[{"value":"7 January 2026","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"10 March 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"3 April 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not Applicable.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"<!--Emphasis Type='Bold' removed-->Consent for publication"}}]}}